Non-Coding RNA最新文献_第9页

Regulation of Macrophage Polarization in Allergy by Noncoding RNAs. 非编码 RNA 对过敏症中巨噬细胞极化的调控

IF 4.3

Non-Coding RNA Pub Date : 2023-12-11 DOI: 10.3390/ncrna9060075

Osamu Ishibashi, Stefan A Muljo, Zohirul Islam

{"title":"Regulation of Macrophage Polarization in Allergy by Noncoding RNAs.","authors":"Osamu Ishibashi, Stefan A Muljo, Zohirul Islam","doi":"10.3390/ncrna9060075","DOIUrl":"10.3390/ncrna9060075","url":null,"abstract":"Allergy is a type 2 immune reaction triggered by antigens known as allergens, including food and environmental substances such as peanuts, plant pollen, fungal spores, and the feces and debris of mites and insects. Macrophages are myeloid immune cells with phagocytic abilities that process exogenous and endogenous antigens. Upon activation, they can produce effector molecules such as cytokines as well as anti-inflammatory molecules. The dysregulation of macrophage function can lead to excessive type 1 inflammation as well as type 2 inflammation, which includes allergic reactions. Thus, it is important to better understand how macrophages are regulated in the pathogenesis of allergies. Emerging evidence highlights the role of noncoding RNAs (ncRNAs) in macrophage polarization, which in turn can modify the pathogenesis of various immune-mediated diseases, including allergies. This review summarizes the current knowledge regarding this topic and considers three classes of ncRNAs: microRNAs, long ncRNAs, and circular ncRNAs. Understanding the roles of these ncRNAs in macrophage polarization will provide new insights into the pathogenesis of allergies and identify potential novel therapeutic targets.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10745746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age and 17β-Estradiol (E2) Facilitate Nuclear Export and Argonaute Loading of microRNAs in the Female Brain 年龄和 17β 雌二醇 (E2) 促进雌性大脑中 microRNA 的核输出和 Argonaute 装载

IF 4.3

Non-Coding RNA Pub Date : 2023-12-06 DOI: 10.3390/ncrna9060074

Megan L. Linscott, Yoldas Yildiz, Sarah Flury, Mikayla L. Newby, Toni R. Pak

{"title":"Age and 17β-Estradiol (E2) Facilitate Nuclear Export and Argonaute Loading of microRNAs in the Female Brain","authors":"Megan L. Linscott, Yoldas Yildiz, Sarah Flury, Mikayla L. Newby, Toni R. Pak","doi":"10.3390/ncrna9060074","DOIUrl":"https://doi.org/10.3390/ncrna9060074","url":null,"abstract":"Aging in women is accompanied by a dramatic change in circulating sex steroid hormones. Specifically, the primary circulating estrogen, 17β-estradiol (E2), is nearly undetectable in post-menopausal women. This decline is associated with a variety of cognitive and mood disorders, yet hormone replacement therapy is only effective within a narrow window of time surrounding the menopausal transition. Our previous work identified microRNAs as a potential molecular substrate underlying the change in E2 efficacy associated with menopause in advanced age. Specifically, we showed that E2 regulated a small subset of mature miRNAs in the aging female brain. In this study, we hypothesized that E2 regulates the stability of mature miRNAs by altering their subcellular localization and their association with argonaute proteins. We also tested the hypothesis that the RNA binding protein, hnRNP A1, was an important regulator of mature miR-9-5p expression in neuronal cells. Our results demonstrated that E2 treatment affected miRNA subcellular localization and its association with argonaute proteins differently, depending on the length of time following E2 deprivation (i.e., ovariectomy). We also provide strong evidence that hnRNP A1 regulates the transcription of pri-miR-9 and likely plays a posttranscriptional role in mature miR-9-5p turnover. Taken together, these data have important implications for considering the optimal timing for hormone replacement therapy, which might be less dependent on age and more related to how long treatment is delayed following menopause.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"43 15","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138597548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis. 龈沟液中的microrna:牙周炎患者差异表达的观察性病例对照研究。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-18 DOI: 10.3390/ncrna9060073

Pedro J Almiñana-Pastor, Francisco M Alpiste-Illueca, Pablo Micó-Martinez, Jose Luis García-Giménez, Eva García-López, Andrés López-Roldán

{"title":"MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis.","authors":"Pedro J Almiñana-Pastor, Francisco M Alpiste-Illueca, Pablo Micó-Martinez, Jose Luis García-Giménez, Eva García-López, Andrés López-Roldán","doi":"10.3390/ncrna9060073","DOIUrl":"10.3390/ncrna9060073","url":null,"abstract":"Objectives: microRNAs (miRNAs) present in the gingival crevicular fluid (GCF) of patients with chronic periodontitis may serve as biomarkers of periodontal disease. The aim of this study was to perform a miRNA-sequencing study of all miRNAs present in GCF, comparing miRNA expression level profiles between advanced chronic periodontitis (CP) patients and healthy subjects (HS).Materials and methods: GCF samples were collected from the single-rooted teeth of patients with severe CP (n = 11) and of HS (n = 12). miRNAs were isolated from GCF using an miRNeasy Serum/Plasma kit(Qiagen GmbH, Hilden, Germany). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression levels of miRNA candidates involved in periodontal pathogenesis.Results: Of all the sequenced miRNAs, miR-199, miR-146a, miR-30a, and miR-338 were identified as best representing the CP patient samples. The validation study identified miR-199 as the most powerful biomarker used to define periodontitis.Conclusions: Upon sequencing all known miRNAs in GCF for the first time, we uncovered several potential biomarkers to define periodontitis. Identifying miRNAS in the GCF using high-throughput approaches will clarify the role of these molecules in periodontitis and provide biomarkers with potential applications.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons. 乙醇和parp介导的核糖体相关长链非编码RNA (lncRNA)在锥体神经元中的调控。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-17 DOI: 10.3390/ncrna9060072

Hooriyah S Rizavi, Hannah E Gavin, Harish R Krishnan, David P Gavin, Rajiv P Sharma

{"title":"Ethanol- and PARP-Mediated Regulation of Ribosome-Associated Long Non-Coding RNA (lncRNA) in Pyramidal Neurons.","authors":"Hooriyah S Rizavi, Hannah E Gavin, Harish R Krishnan, David P Gavin, Rajiv P Sharma","doi":"10.3390/ncrna9060072","DOIUrl":"10.3390/ncrna9060072","url":null,"abstract":"Although, by definition, long noncoding RNAs (lncRNAs) are not translated, they are sometimes associated with ribosomes. In fact, some estimates suggest the existence of more than 50 K lncRNA molecules that could encode for small peptides. We examined the effects of an ethanol and Poly-ADP Ribose Polymerase (PARP) inhibitor (ABT-888) on ribosome-bound lncRNAs. Mice were administered via intraperitoneal injection (i.p.) either normal saline (CTL) or ethanol (EtOH) twice a day for four consecutive days. On the fourth day, a sub-group of mice administered with ethanol also received ABT-888 (EtOH+ABT). Ribosome-bound lncRNAs in CaMKIIα-expressing pyramidal neurons were measured using the Translating Ribosome Affinity Purification (TRAP) technique. Our findings show that EtOH altered the attachment of 107 lncRNA transcripts, while EtOH+ABT altered 60 lncRNAs. Among these 60 lncRNAs, 49 were altered by both conditions, while EtOH+ABT uniquely altered the attachment of 11 lncRNA transcripts that EtOH alone did not affect. To validate these results, we selected eight lncRNAs (Mir124-2hg, 5430416N02Rik, Snhg17, Snhg12, Snhg1, Mir9-3hg, Gas5, and 1110038B12Rik) for qRT-PCR analysis. The current study demonstrates that ethanol-induced changes in lncRNA attachment to ribosomes can be mitigated by the addition of the PARP inhibitor ABT-888.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LADON, a Natural Antisense Transcript of NODAL, Promotes Tumour Progression and Metastasis in Melanoma. LADON是一种天然的NODAL反义转录物，促进黑色素瘤的肿瘤进展和转移。

IF 4.3

Non-Coding RNA Pub Date : 2023-11-15 DOI: 10.3390/ncrna9060071

Annie Dutriaux, Serena Diazzi, Chiara Bresesti, Sylvie Hardouin, Frédérique Deshayes, Jérôme Collignon, Domenico Flagiello

{"title":"LADON, a Natural Antisense Transcript of NODAL, Promotes Tumour Progression and Metastasis in Melanoma.","authors":"Annie Dutriaux, Serena Diazzi, Chiara Bresesti, Sylvie Hardouin, Frédérique Deshayes, Jérôme Collignon, Domenico Flagiello","doi":"10.3390/ncrna9060071","DOIUrl":"10.3390/ncrna9060071","url":null,"abstract":"The TGFβ family member NODAL, repeatedly required during embryonic development, has also been associated with tumour progression. Our aim was to clarify the controversy surrounding its involvement in melanoma tumour progression. We found that the deletion of the NODAL exon 2 in a metastatic melanoma cell line impairs its ability to form tumours and colonize distant tissues. However, we show that this phenotype does not result from the absence of NODAL, but from a defect in the expression of a natural antisense transcript of NODAL, here called LADON. We show that LADON expression is specifically activated in metastatic melanoma cell lines, that its transcript is packaged in exosomes secreted by melanoma cells, and that, via its differential impact on the expression of oncogenes and tumour suppressors, it promotes the mesenchymal to amoeboid transition that is critical for melanoma cell invasiveness. LADON is, therefore, a new player in the regulatory network governing tumour progression in melanoma and possibly in other types of cancer.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"9 6","pages":""},"PeriodicalIF":4.3,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10661258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138176917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Overview of the Immune Modulatory Properties of Long Non-Coding RNAs and Their Potential Use as Therapeutic Targets in Cancer 长链非编码rna的免疫调节特性及其作为癌症治疗靶点的潜在应用综述

Non-Coding RNA Pub Date : 2023-11-11 DOI: 10.3390/ncrna9060070

Moises Martinez-Castillo, Abdelrahman M. Elsayed, Gabriel López-Berestein, Paola Amero, Cristian Rodríguez-Aguayo

引用次数: 0

The Typical tRNA Co-Expresses Multiple 5′ tRNA Halves Whose Sequences and Abundances Depend on Isodecoder and Isoacceptor and Change with Tissue Type, Cell Type, and Disease 典型的tRNA共表达多个5 ' tRNA半部分，其序列和丰度取决于同位解码器和同位受体，并随组织类型、细胞类型和疾病而变化

Non-Coding RNA Pub Date : 2023-11-06 DOI: 10.3390/ncrna9060069

Robert Brian Akins, Kayleigh Ostberg, Tess Cherlin, Nikolas J. Tsiouplis, Phillipe Loher, Isidore Rigoutsos

{"title":"The Typical tRNA Co-Expresses Multiple 5′ tRNA Halves Whose Sequences and Abundances Depend on Isodecoder and Isoacceptor and Change with Tissue Type, Cell Type, and Disease","authors":"Robert Brian Akins, Kayleigh Ostberg, Tess Cherlin, Nikolas J. Tsiouplis, Phillipe Loher, Isidore Rigoutsos","doi":"10.3390/ncrna9060069","DOIUrl":"https://doi.org/10.3390/ncrna9060069","url":null,"abstract":"Transfer RNA-derived fragments (tRFs) are noncoding RNAs that arise from either mature transfer RNAs (tRNAs) or their precursors. One important category of tRFs comprises the tRNA halves, which are generated through cleavage at the anticodon. A given tRNA typically gives rise to several co-expressed 5’-tRNA halves (5′-tRHs) that differ in the location of their 3′ ends. These 5′-tRHs, even though distinct, have traditionally been treated as indistinguishable from one another due to their near-identical sequences and lengths. We focused on co-expressed 5′-tRHs that arise from the same tRNA and systematically examined their exact sequences and abundances across 10 different human tissues. To this end, we manually curated and analyzed several hundred human RNA-seq datasets from NCBI’s Sequence Run Archive (SRA). We grouped datasets from the same tissue into their own collection and examined each group separately. We found that a given tRNA produces different groups of co-expressed 5′-tRHs in different tissues, different cell lines, and different diseases. Importantly, the co-expressed 5′-tRHs differ in their sequences, absolute abundances, and relative abundances, even among tRNAs with near-identical sequences from the same isodecoder or isoacceptor group. The findings suggest that co-expressed 5′-tRHs that are produced from the same tRNA or closely related tRNAs have distinct, context-dependent roles. Moreover, our analyses show that cell lines modeling the same tissue type and disease may not be interchangeable when it comes to experimenting with tRFs.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"7 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135589756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulation of Non-Coding RNAs: Roles of miRNAs and lncRNAs in the Pathogenesis of Multiple Myeloma 非编码rna的失调:mirna和lncrna在多发性骨髓瘤发病中的作用

Non-Coding RNA Pub Date : 2023-11-03 DOI: 10.3390/ncrna9060068

Nor Hayati Ismail, Ali Mussa, Mutaz Jamal Al-Khreisat, Shafini Mohamed Yusoff, Azlan Husin, Hamid Ali Nagi Al-Jamal, Muhammad Farid Johan, Md Asiful Islam

{"title":"Dysregulation of Non-Coding RNAs: Roles of miRNAs and lncRNAs in the Pathogenesis of Multiple Myeloma","authors":"Nor Hayati Ismail, Ali Mussa, Mutaz Jamal Al-Khreisat, Shafini Mohamed Yusoff, Azlan Husin, Hamid Ali Nagi Al-Jamal, Muhammad Farid Johan, Md Asiful Islam","doi":"10.3390/ncrna9060068","DOIUrl":"https://doi.org/10.3390/ncrna9060068","url":null,"abstract":"The dysregulation of non-coding RNAs (ncRNAs), specifically microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), leads to the development and advancement of multiple myeloma (MM). miRNAs, in particular, are paramount in post-transcriptional gene regulation, promoting mRNA degradation and translational inhibition. As a result, miRNAs can serve as oncogenes or tumor suppressors depending on the target genes. In MM, miRNA disruption could result in abnormal gene expression responsible for cell growth, apoptosis, and other biological processes pertinent to cancer development. The dysregulated miRNAs inhibit the activity of tumor suppressor genes, contributing to disease progression. Nonetheless, several miRNAs are downregulated in MM and have been identified as gene regulators implicated in extracellular matrix remodeling and cell adhesion. miRNA depletion potentially facilitates the tumor advancement and resistance of therapeutic drugs. Additionally, lncRNAs are key regulators of numerous cellular processes, such as gene expression, chromatin remodeling, protein trafficking, and recently linked MM development. The lncRNAs are uniquely expressed and influence gene expression that supports MM growth, in addition to facilitating cellular proliferation and viability via multiple molecular pathways. miRNA and lncRNA alterations potentially result in anomalous gene expression and interfere with the regular functioning of MM. Thus, this review aims to highlight the dysregulation of these ncRNAs, which engender novel therapeutic modalities for the treatment of MM.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"22 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135873491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus 肥胖症外泌体hsa-miR-551b-3p的下调及其与2型糖尿病的关系

Non-Coding RNA Pub Date : 2023-11-02 DOI: 10.3390/ncrna9060067

Kseniia V. Dracheva, Irina A. Pobozheva, Kristina A. Anisimova, Stanislav G. Balandov, Maria N. Grunina, Zarina M. Hamid, Dmitriy I. Vasilevsky, Sofya N. Pchelina, Valentina V. Miroshnikova

{"title":"Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus","authors":"Kseniia V. Dracheva, Irina A. Pobozheva, Kristina A. Anisimova, Stanislav G. Balandov, Maria N. Grunina, Zarina M. Hamid, Dmitriy I. Vasilevsky, Sofya N. Pchelina, Valentina V. Miroshnikova","doi":"10.3390/ncrna9060067","DOIUrl":"https://doi.org/10.3390/ncrna9060067","url":null,"abstract":"Obesity is a significant risk factor for the development of type 2 diabetes mellitus (T2DM). Adipose tissue dysfunction can affect the pool of circulating exosomal miRNAs, driving concomitant disease in obesity. These exosomal miRNAs can reflect adipose tissue functionality, thus serving as prognostic biomarkers for disease monitoring in case of T2DM. In the present study, we conducted NanoString microRNA profiling of extracellular vesicles (EVs) secreted by adipose tissue of obese patients (body mass index (BMI) > 35) without T2DM and nonobese individuals (BMI < 30) as a control group. Functional and pathway enrichment analysis showed that miRNAs associated with obesity in this study were implicated in insulin signaling and insulin resistance biological pathways. Further, these microRNAs were screened in serum EVs in the following groups: (1) obese patients with T2DM, (2) obese patients without T2DM, and (3) nonobese individuals as a control group. has-miR-551b-3p was shown to be downregulated in adipose tissue EVs, as well as in serum EVs, of patients with obesity without T2DM. At the same time, the serum exosomal hsa-miR-551b-3p content was significantly higher in obese patients with T2DM when compared with obese patients without T2DM and may be a potential biomarker of T2DM development in obesity.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"2 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135974074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of Novel miRNAs in Colorectal Cancer: Potential Biological Roles and Clinical Utility 结直肠癌中新型mirna的发现:潜在的生物学作用和临床应用

Non-Coding RNA Pub Date : 2023-10-26 DOI: 10.3390/ncrna9060065

Iael Weissberg Minutentag, Ana Laura Seneda, Mateus C. Barros-Filhos, Márcio de Carvalho, Vanessa G. P. Souza, Claudia N. Hasimoto, Marcelo P. T. Moraes, Fabio A. Marchi, Wan L. Lam, Patricia P. Reis, Sandra A. Drigo

{"title":"Discovery of Novel miRNAs in Colorectal Cancer: Potential Biological Roles and Clinical Utility","authors":"Iael Weissberg Minutentag, Ana Laura Seneda, Mateus C. Barros-Filhos, Márcio de Carvalho, Vanessa G. P. Souza, Claudia N. Hasimoto, Marcelo P. T. Moraes, Fabio A. Marchi, Wan L. Lam, Patricia P. Reis, Sandra A. Drigo","doi":"10.3390/ncrna9060065","DOIUrl":"https://doi.org/10.3390/ncrna9060065","url":null,"abstract":"Deregulated miRNAs are associated with colorectal cancer (CRC), with alterations depending on the tumor location. Novel tissue-specific miRNAs have been identified in different tumors and are associated with cancer. We used miRMaster to identify novel miRNAs in CRC from the TCGA and GEO data (discovery and validation groups). We used TCGA data from five tissues to analyze miRNA tissue specificity. miRDB was used to predict miRNA targets, and the UCSC Xena Browser was used to evaluate target expression. After successive analyses, we identified 15 novel miRNAs with the same expression patterns in CRC in both the discovery and validation groups. Four molecules (nov-miR-13844-5p, nov-miR-7154-5p, nov-miR-5035-3p, and nov-miR-590-5p) were differentially expressed in proximal and distal CRC. The nov-miR-3345-5p and nov-miR-13172-3p, which are upregulated in tumors, are only expressed in colorectal tissues. These molecules have been linked to a worse prognosis in right-sided colon and rectal carcinomas. An analysis revealed an association between eight novel miRNAs and 81 targets, mostly cancer-related genes, with varying expression based on tumor location. These findings provide new miRNAs with potential biological relevance, molecular biomarkers, and therapeutic targets for CRC treatment.","PeriodicalId":19271,"journal":{"name":"Non-Coding RNA","volume":"35 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134908660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0