Circulating miRNAs in the Plasma of Post-COVID-19 Patients with Typical Recovery and Those with Long-COVID Symptoms: Regulation of Immune Response-Associated Pathways.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anna M Timofeeva, Artem O Nikitin, Georgy A Nevinsky
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引用次数: 0

Abstract

Following the acute phase of SARS-CoV-2 infection, certain individuals experience persistent symptoms referred to as long COVID. This study analyzed the patients categorized into three distinct groups: (1) individuals presenting rheumatological symptoms associated with long COVID, (2) patients who have successfully recovered from COVID-19, and (3) donors who have never contracted COVID-19. A notable decline in the expression of miR-200c-3p, miR-766-3p, and miR-142-3p was identified among patients exhibiting rheumatological symptoms of long COVID. The highest concentration of miR-142-3p was found in healthy donors. One potential way to reduce miRNA concentrations is through antibody-mediated hydrolysis. Not only can antibodies possessing RNA-hydrolyzing activity recognize the miRNA substrate specifically, but they also catalyze its hydrolysis. The analysis of the catalytic activity of plasma antibodies revealed that antibodies from patients with long COVID demonstrated lower hydrolysis activity against five fluorescently labeled oligonucleotide sequences corresponding to the Flu-miR-146b-5p, Flu-miR-766-3p, Flu-miR-4742-3p, and Flu-miR-142-3p miRNAs and increased activity against the Flu-miR-378a-3p miRNA compared to other patient groups. The changes in miRNA concentrations and antibody-mediated hydrolysis of miRNAs are assumed to have a complex regulatory mechanism that is linked to gene pathways associated with the immune system. We demonstrate that all six miRNAs under analysis are associated with a large number of signaling pathways associated with immune response-associated pathways.

COVID-19后典型恢复期和长期COVID症状患者血浆中的循环miRNA:免疫反应相关通路的调控。
在感染 SARS-CoV-2 的急性期后,某些人会出现持续性症状,称为长 COVID。本研究将患者分为三类进行分析:(1)出现与长COVID相关的风湿病症状的人;(2)从COVID-19中成功康复的患者;(3)从未感染过COVID-19的捐献者。研究发现,在出现长期 COVID 风湿症状的患者中,miR-200c-3p、miR-766-3p 和 miR-142-3p 的表达量明显下降。健康供体中的 miR-142-3p 浓度最高。降低 miRNA 浓度的一个潜在方法是通过抗体介导的水解作用。具有 RNA 水解活性的抗体不仅能特异性识别 miRNA 底物,还能催化其水解。对血浆抗体催化活性的分析表明,与其他患者组相比,长COVID患者的抗体对对应于Flu-miR-146b-5p、Flu-miR-766-3p、Flu-miR-4742-3p和Flu-miR-142-3p miRNA的五个荧光标记寡核苷酸序列的水解活性较低,而对Flu-miR-378a-3p miRNA的水解活性较高。miRNA浓度的变化和抗体介导的miRNA水解被认为具有复杂的调节机制,与免疫系统相关的基因通路有关。我们证明,所分析的所有六种 miRNA 都与大量与免疫反应相关的信号通路有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Non-Coding RNA
Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
6.70
自引率
4.70%
发文量
74
审稿时长
10 weeks
期刊介绍: Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.
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