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Nucleosides & nucleotides最新文献

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Inhibition of HIV-1 integration by mono- & bi-functionalized triple helix forming oligonucleotides. 单双功能化三螺旋形成寡核苷酸对HIV-1整合的抑制作用。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044831
P Brodin, M Gottikh, C Auclair, J F Mouscadet
{"title":"Inhibition of HIV-1 integration by mono- & bi-functionalized triple helix forming oligonucleotides.","authors":"P Brodin,&nbsp;M Gottikh,&nbsp;C Auclair,&nbsp;J F Mouscadet","doi":"10.1080/07328319908044831","DOIUrl":"https://doi.org/10.1080/07328319908044831","url":null,"abstract":"<p><p>HIV-1 DNA integration is carried out by integrase, a viral protein which binds to specific sequences located on both extremities of the HIV-1 DNA LTR. Inhibition of integration was observed with submicromolar concentrations of mono- or bifunctionalized 11-mer oligonucleotide-intercalators, which were designed to form an alternate strand triple helix with the U5 LTR end containing two adjacent purine tracts on opposite strands 5'-GGAAAATCTCT-3'/3'-CCTTTTAGAGA-5'.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1717-8"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Uptake of oligonucleotides by keratinocytes. 角质形成细胞对寡核苷酸的摄取。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044826
P Laktionov, J E Dazard, J Piette, E Vives, E Rykova, V Vlassov, B Lebleu
{"title":"Uptake of oligonucleotides by keratinocytes.","authors":"P Laktionov,&nbsp;J E Dazard,&nbsp;J Piette,&nbsp;E Vives,&nbsp;E Rykova,&nbsp;V Vlassov,&nbsp;B Lebleu","doi":"10.1080/07328319908044826","DOIUrl":"https://doi.org/10.1080/07328319908044826","url":null,"abstract":"<p><p>Oligonucleotides (ODNs) conjugated to rhodamin (Rh) and 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODNs transport into keratinocytes. Affinity labeling of two proteins, 63 and 35 kDa, and the inhibition of the affinity labeling and ODNs uptake by the cells in the presence of nucleic acids, polyanions and trypsin suggest, that the proteins are involved in transport of nucleic acids in keratinocytes.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1697-9"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Antiviral activity of magnesium and magnesium/poly r(A-U) combinations against two RNA viruses. 镁和镁/聚r(A-U)组合对两种RNA病毒的抗病毒活性。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044668
J Jamison, J Gilloteaux, M R Nassiri, C C Tsai, J Summers
{"title":"Antiviral activity of magnesium and magnesium/poly r(A-U) combinations against two RNA viruses.","authors":"J Jamison,&nbsp;J Gilloteaux,&nbsp;M R Nassiri,&nbsp;C C Tsai,&nbsp;J Summers","doi":"10.1080/07328319908044668","DOIUrl":"https://doi.org/10.1080/07328319908044668","url":null,"abstract":"<p><p>Magnesium (Mg2+) potentiated the anti-vesicular stomatitis virus (VSV) activity of poly r(A-U) or poly r(G-C) and the anti-HIV-1 activity of poly r(A-U). Mg2+ did not affect the anti-VSV activity of poly (rI).poly (rC), poly (dA-dT).poly (dA-dT) or poly (dG-dC).poly (dG-dC). Modulation of one or more nuclear (nucleolar) processes of the host cell may be responsible for the synergistic antiviral activity.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1221-2"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Making drugs out of oligonucleotides: a brief review and perspective. 从寡核苷酸制备药物:简要回顾和展望。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044652
P D Cook
{"title":"Making drugs out of oligonucleotides: a brief review and perspective.","authors":"P D Cook","doi":"10.1080/07328319908044652","DOIUrl":"https://doi.org/10.1080/07328319908044652","url":null,"abstract":"<p><p>I provide a brief review and perspective thoughts concerning the antisense oligonucleotide, drug discovery paradigm.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1141-62"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
High-density nucleoside analog probe arrays for enhanced hybridization. 用于增强杂交的高密度核苷模拟探针阵列。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044695
J A Fidanza, G H McGall
{"title":"High-density nucleoside analog probe arrays for enhanced hybridization.","authors":"J A Fidanza,&nbsp;G H McGall","doi":"10.1080/07328319908044695","DOIUrl":"https://doi.org/10.1080/07328319908044695","url":null,"abstract":"<p><p>DNA probe arrays were synthesized with analogs of 2,6-diaminopurine and 2'-O-methyl-thymidine in place of A and T. AT-rich GeneChip test arrays containing 14-mer or 20-mer analog probes improved hybridization to fluorescently-labeled RNA sequences under stringent conditions.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1293-5"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E. coli. 用于大肠杆菌核苷酸切除修复研究的修饰DNA片段的合成及生物学评价。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044710
V Monaco, K I van de Wetering, N J Meeuwenoord, H A van den Elst, H R Stuivenberg, R Visse, J C van der Kaaden, G F Moolenaar, E E Verhoeven, N Goosen, G A van der Marel, J H van Boom
{"title":"Synthesis and biological evaluation of modified DNA fragments for the study of nucleotide excision repair in E. coli.","authors":"V Monaco,&nbsp;K I van de Wetering,&nbsp;N J Meeuwenoord,&nbsp;H A van den Elst,&nbsp;H R Stuivenberg,&nbsp;R Visse,&nbsp;J C van der Kaaden,&nbsp;G F Moolenaar,&nbsp;E E Verhoeven,&nbsp;N Goosen,&nbsp;G A van der Marel,&nbsp;J H van Boom","doi":"10.1080/07328319908044710","DOIUrl":"https://doi.org/10.1080/07328319908044710","url":null,"abstract":"<p><p>Three new cholesterol-containing phosphoramidites where synthesized and used in automated synthesis of modified DNA fragments. These cholesterol lesions are good substrates for the E. coli UvrABC endonuclease. In vitro they are incised from damaged DNA with higher efficiency in respect with the cholesterol lesions previously published.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1339-41"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044710","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
The cytotoxicity of anti-PAI-1 oligonucleotides and their conjugates. 抗pai -1寡核苷酸及其偶联物的细胞毒性。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044829
A Kobylańska, E Pluskota, Z Pawłowska, A Okruszek, C S Cierniewski, W J Stec
{"title":"The cytotoxicity of anti-PAI-1 oligonucleotides and their conjugates.","authors":"A Kobylańska,&nbsp;E Pluskota,&nbsp;Z Pawłowska,&nbsp;A Okruszek,&nbsp;C S Cierniewski,&nbsp;W J Stec","doi":"10.1080/07328319908044829","DOIUrl":"https://doi.org/10.1080/07328319908044829","url":null,"abstract":"<p><p>The cytotoxicity of anti-PAI-5 hexadecanucleotides (phosphodiesters and phosphorothioates) and their conjugates with lipophilic alcohols was tested in EA.hy 926 hybrid endothelial cells. Some cytotoxicity was found for cholesteryl and bornyl conjugates at concentrations higher than those used for antisense inhibition experiments.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1709-10"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044829","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
In vitro and in vivo anti-influenza A virus activity of antisense oligonucleotides. 体外和体内抗甲型流感病毒的反义寡核苷酸活性。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044823
T Abe, T Mizuta, S Suzuki, T Hatta, K Takai, T Yokota, H Takaku
{"title":"In vitro and in vivo anti-influenza A virus activity of antisense oligonucleotides.","authors":"T Abe,&nbsp;T Mizuta,&nbsp;S Suzuki,&nbsp;T Hatta,&nbsp;K Takai,&nbsp;T Yokota,&nbsp;H Takaku","doi":"10.1080/07328319908044823","DOIUrl":"https://doi.org/10.1080/07328319908044823","url":null,"abstract":"<p><p>We have demonstrated that antisense phosphorothioate oligonucleotides (S-ODNs) inhibit influenza virus A replication in MDCK cells. The liposomally encapsulated and the free antisense phosphorothioate oligonucleotides with four target sites (PB1, PB2, PA, and NP) were tested for their abilities to inhibit virus-induced cytopathogenic effects by a MTT assay using MDCK cells. The liposomally encapsulated S-ODN complementary to the sites of the PB2-AUG initiation codon showed highly inhibitory effects. Therefore, the antiviral effects of S-ODN-PB2-AUG and PA-AUG were examined in a mouse model of influenza virus A infection. PB2-AUG oligomer treated i.v. significantly prolonged the mean survival time in day (MDS) and increased the survival rates with does dependent manner.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1685-8"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Nucleoside modifications affect the structure and stability of the anticodon of tRNA(Lys,3). 核苷修饰影响tRNA反密码子的结构和稳定性(Lys,3)。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044790
D R Davis, P C Durant
{"title":"Nucleoside modifications affect the structure and stability of the anticodon of tRNA(Lys,3).","authors":"D R Davis,&nbsp;P C Durant","doi":"10.1080/07328319908044790","DOIUrl":"https://doi.org/10.1080/07328319908044790","url":null,"abstract":"<p><p>NMR spectroscopy was used to determine the solution structures of RNA oligonucleotides comprising the anticodon domain of tRNA(Lys,3). The structural effects of the pseudouridine modification at position 39 were investigated and are well correlated with changes in thermodynamic parameters. The loop conformation differs from that seen in tRNA(Phe) and provides an explanation of the critical role of modification in this tRNA.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1579-81"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044790","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Synthesis of modified thiopurine nucleosides for structural characterization of human thiopurine S-methyltransferase. 人硫嘌呤s -甲基转移酶结构表征的修饰硫嘌呤核苷的合成。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044839
G A Korshunova, E Y Krynetski, M T Mtchedlidze, D V Agapkin, W E Evans, N F Krynetskaia
{"title":"Synthesis of modified thiopurine nucleosides for structural characterization of human thiopurine S-methyltransferase.","authors":"G A Korshunova,&nbsp;E Y Krynetski,&nbsp;M T Mtchedlidze,&nbsp;D V Agapkin,&nbsp;W E Evans,&nbsp;N F Krynetskaia","doi":"10.1080/07328319908044839","DOIUrl":"https://doi.org/10.1080/07328319908044839","url":null,"abstract":"<p><p>Synthesis of a number of photoactive thiopurine-containing nucleosides was described. S-methylation of the synthesized compounds in the course of the reaction catalyzed by recombinant human thiopurine S-methyltransferase was studied by UV-spectroscopy.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1747-8"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044839","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21337935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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