P Laktionov, J E Dazard, J Piette, E Vives, E Rykova, V Vlassov, B Lebleu
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引用次数: 8
摘要
利用偶联罗丹明(Rh)和4-[(n -2-氯乙基- n -甲基)氨基]苄胺的寡核苷酸(ODNs)来研究ODNs在角化细胞中的转运。63和35 kDa两种蛋白的亲和标记，以及在核酸、多阴离子和胰蛋白酶存在下细胞对亲和标记和odn摄取的抑制表明，这两种蛋白参与了角化细胞中核酸的运输。
Oligonucleotides (ODNs) conjugated to rhodamin (Rh) and 4-[(N-2-chloroethyl-N-methyl)amino] benzylamine were used to investigate ODNs transport into keratinocytes. Affinity labeling of two proteins, 63 and 35 kDa, and the inhibition of the affinity labeling and ODNs uptake by the cells in the presence of nucleic acids, polyanions and trypsin suggest, that the proteins are involved in transport of nucleic acids in keratinocytes.
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