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Nucleosides & nucleotides最新文献

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Solid-supported oligonucleotide systems for special biomedical applications. 特殊生物医学应用的固体支撑寡核苷酸系统。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044698
H Seliger, M Hinz, S Gura, B Nitzan, S Margel
{"title":"Solid-supported oligonucleotide systems for special biomedical applications.","authors":"H Seliger,&nbsp;M Hinz,&nbsp;S Gura,&nbsp;B Nitzan,&nbsp;S Margel","doi":"10.1080/07328319908044698","DOIUrl":"https://doi.org/10.1080/07328319908044698","url":null,"abstract":"<p><p>The use of composite beads consisting of a 6 microns polystyrene core with 30 nm surface-bound silica particles to routine automatic oligodeoxynucleotide (ODN) synthesis is described.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1305-7"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Mechanism and specificity of RNA cleavage by chemical ribonucleases. 化学核糖核酸酶切割RNA的机制和特异性。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044753
N Beloglazova, A Vlassov, D Konevetc, V Sil'nikov, M Zenkova, R Giege, V Vlassov
{"title":"Mechanism and specificity of RNA cleavage by chemical ribonucleases.","authors":"N Beloglazova,&nbsp;A Vlassov,&nbsp;D Konevetc,&nbsp;V Sil'nikov,&nbsp;M Zenkova,&nbsp;R Giege,&nbsp;V Vlassov","doi":"10.1080/07328319908044753","DOIUrl":"https://doi.org/10.1080/07328319908044753","url":null,"abstract":"<p><p>Cleaving of model RNA substrates by chemical ribonucleases constructed by conjugation of 1,4 diazabicyclo[2,2,2]octane with histamine and histidine was investigated. Similarly to RNase A, the chemical RNases produce fragments with 5' hydroxy-group and 3'-cyclophosphate. The cleavage occurs as the catalytic reaction: more than 150 phosphodiester bonds in RNA can be cleaved by one molecule of RNase mimic.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1463-5"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044753","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Thermodynamic studies on hairpin forming antisense-oligodeoxynucleotides directed against hepatitis C virus RNA. 针对丙型肝炎病毒RNA的发夹形成反义寡脱氧核苷酸的热力学研究。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044824
T J Lehmann, S Eisenhardt, J W Engels
{"title":"Thermodynamic studies on hairpin forming antisense-oligodeoxynucleotides directed against hepatitis C virus RNA.","authors":"T J Lehmann,&nbsp;S Eisenhardt,&nbsp;J W Engels","doi":"10.1080/07328319908044824","DOIUrl":"https://doi.org/10.1080/07328319908044824","url":null,"abstract":"<p><p>Phosphorothioate and benzyl-modified antisense-oligodeoxynucleotides directed against nucleotides 334-350 of the Hepatitis C Virus RNA form surprisingly stable hairpins. These data contribute to solve a structural detail information in search for a global secondary structure model of the Non Coding Region (NCR) of HCV.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1689-91"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Using fluorescence resonance energy transfer (FRET) for measuring 2-5A analogues ability to activate RNase L. 利用荧光共振能量转移(FRET)测定2-5A类似物激活RNase L的能力。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044776
H Cramer, D A Geselowitz, P F Torrence
{"title":"Using fluorescence resonance energy transfer (FRET) for measuring 2-5A analogues ability to activate RNase L.","authors":"H Cramer,&nbsp;D A Geselowitz,&nbsp;P F Torrence","doi":"10.1080/07328319908044776","DOIUrl":"https://doi.org/10.1080/07328319908044776","url":null,"abstract":"<p><p>The development of a method for measuring the ability of 2-5A analogues to activate the cleavage of an oligoribonucleotide substrate by RNase L is described. This method is based on fluorescence resonance energy transfer. The method is easily performed with 96-well plates, allowing for quantitative high-throughput analyses of 2-5A analogues under different reaction conditions.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1523-5"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044776","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Tc-99m-labeling of modified RNA. tc -99m标记修饰RNA。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044759
C S Hilger, M C Willis, M Wolters, W A Pieken
{"title":"Tc-99m-labeling of modified RNA.","authors":"C S Hilger,&nbsp;M C Willis,&nbsp;M Wolters,&nbsp;W A Pieken","doi":"10.1080/07328319908044759","DOIUrl":"https://doi.org/10.1080/07328319908044759","url":null,"abstract":"<p><p>The synthesis of Tc-99m-labeled, modified RNA is reported. This new class of radiopharmaceuticals is of potential interest as target specific imaging agents. The preparation of N3S-RNA was achieved by coupling protected MAG2-units to amino modified ON's. The N3S-RNA was Tc-99m-labeled with 90-95% radiochemical yield and specific activities of 37MBq/nmol leading to 1:1-Tc-99m-N3S-aptamers.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1479-81"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044759","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Sequence-specific alkylation of DNA by duocarmycin A and its novel derivatives bearing PY/IM polyamides. 多霉素A及其含有PY/IM聚酰胺的新型衍生物的DNA序列特异性烷基化。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044799
Z F Tao, T Fujiwara, I Saito, H Sugiyama
{"title":"Sequence-specific alkylation of DNA by duocarmycin A and its novel derivatives bearing PY/IM polyamides.","authors":"Z F Tao,&nbsp;T Fujiwara,&nbsp;I Saito,&nbsp;H Sugiyama","doi":"10.1080/07328319908044799","DOIUrl":"https://doi.org/10.1080/07328319908044799","url":null,"abstract":"<p><p>A new class of sequence-specific DNA alkylating agents were developed based on the reactivity of duocarmycin A and the DNA-reading ability of pyrrole-imidazole polyamide. The DNA alkylation sequence specificity by duocarmycin A can be modulated by a variety of pyrrole-imidazole triamides in a predictable manner. Novel hybrids of the segment A of duocarmycin A and pyrrole-imidazole polyamides efficiently and highly selectively alkylated the target base possessing match sequences of Dervan's binding code.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1615-6"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Formation of triple helices at irregular poly (R.Y) sites located in critical positions in the human bcr promoter. 在人类bcr启动子关键位置的不规则聚(R.Y)位点形成三螺旋。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044792
L E Xodo, E Del Terra, B Scaggiante, G Manzini, F Quadrifoglio
{"title":"Formation of triple helices at irregular poly (R.Y) sites located in critical positions in the human bcr promoter.","authors":"L E Xodo,&nbsp;E Del Terra,&nbsp;B Scaggiante,&nbsp;G Manzini,&nbsp;F Quadrifoglio","doi":"10.1080/07328319908044792","DOIUrl":"https://doi.org/10.1080/07328319908044792","url":null,"abstract":"<p><p>Two polypurine sequences interrupted respectively by one and two adjacent pyrimidines have been identified in the promoter of the human bcr gene. Although these targets are irregular they are recognised and tightly bound by AG and GT motif triplex-forming oligonucleotides. Thermodynamic and kinetic data are presented.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1587-92"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044792","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specifically designed polymeric nanospheres increase cellular uptake of unmodified antisense ODNs. 特别设计的聚合物纳米球增加了未修饰的反义odn的细胞摄取。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044820
L Tondelli, M Laus, A Ricca, G Citro
{"title":"Specifically designed polymeric nanospheres increase cellular uptake of unmodified antisense ODNs.","authors":"L Tondelli,&nbsp;M Laus,&nbsp;A Ricca,&nbsp;G Citro","doi":"10.1080/07328319908044820","DOIUrl":"https://doi.org/10.1080/07328319908044820","url":null,"abstract":"<p><p>The cellular uptake and the inhibitory effect of c-myb unmodified antisense oligonucleotides reversibly bound to new polymeric nanoparticles in HL-60 cellular system have been found to increase by 50 folds if compared with the free ODN. An initial single dose (320 nM) of the nanoparticle bound unmodified antimyb ODN has been able to specifically inhibit HL-60 leukemia cell proliferation for at least 8 days.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1677-9"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044820","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Uptake and intracellular distribution of oligonucleotides vectorized by a PAMAM dendrimer. 由PAMAM树状大分子矢量化的寡核苷酸的摄取和细胞内分布。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044833
V Hélin, M Gottikh, Z Mishal, F Subra, C Malvy, M Lavignon
{"title":"Uptake and intracellular distribution of oligonucleotides vectorized by a PAMAM dendrimer.","authors":"V Hélin,&nbsp;M Gottikh,&nbsp;Z Mishal,&nbsp;F Subra,&nbsp;C Malvy,&nbsp;M Lavignon","doi":"10.1080/07328319908044833","DOIUrl":"https://doi.org/10.1080/07328319908044833","url":null,"abstract":"<p><p>We studied the uptake and intracellular distribution of an FITC labelled phosphodiester oligodeoxynucleotide (ODN) vectorized by a dendrimeric structure in cell culture.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1721-2"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044833","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21337934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Synthesis and biological activity of 2',5'-oligoadenylate trimers containing 5'-terminal 5'-amino-5'-deoxy- and 5'-amino-3',5'-dideoxyadenosine derivatives. 含5′端5′-氨基-5′-脱氧和5′-氨基-3′，5′-二脱氧腺苷衍生物的2′，5′-低聚腺苷酸三聚体的合成及生物活性
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044760
E I Kvasyuk, I A Mikhailopulo, R J Suhadolnik, E E Henderson, N F Muto, K T Iacono, J Homon, W Pfleiderer
{"title":"Synthesis and biological activity of 2',5'-oligoadenylate trimers containing 5'-terminal 5'-amino-5'-deoxy- and 5'-amino-3',5'-dideoxyadenosine derivatives.","authors":"E I Kvasyuk,&nbsp;I A Mikhailopulo,&nbsp;R J Suhadolnik,&nbsp;E E Henderson,&nbsp;N F Muto,&nbsp;K T Iacono,&nbsp;J Homon,&nbsp;W Pfleiderer","doi":"10.1080/07328319908044760","DOIUrl":"https://doi.org/10.1080/07328319908044760","url":null,"abstract":"<p><p>Some new 2',5'-oligonucleotide trimers containing 5'-terminal 5'-amino-5'-deoxy- and 5'-amino-3',5'-dideoxyadenosine derivatives have been synthesized. Some of the trimers showed biological inhibitions of HIV-1 reverse transcriptase (RT), HIV-1 induced syncytia formation and PCR amplification.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1483-4"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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