Nucleosides & nucleotides最新文献_第6页

Chemical synthesis of oligonucleotides containing the (6-4) photoproduct at the thymine-cytosine site and its repair by (6-4) photolyase. 胸腺嘧啶-胞嘧啶位点上含有(6-4)光产物的寡核苷酸的化学合成及其(6-4)光解酶的修复。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044706

S Iwai, T Mizukoshi, K Hitomi, T Todo

引用次数: 2

New techniques for the rapid characterization of oligonucleotides by mass spectrometry. 质谱法快速表征寡核苷酸的新技术。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044782

J A McCloskey, A B Whitehill, J Rozenski, F Qiu, P F Crain

引用次数: 22

Detection of nucleic acids by cycling probe technology on magnetic particles: high sensitivity and ease of separation. 磁颗粒循环探针技术检测核酸:灵敏度高，易于分离。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044696

R Bhatt, B Scott, S Whitney, R N Bryan, L Cloney, A Lebedev

{"title":"Detection of nucleic acids by cycling probe technology on magnetic particles: high sensitivity and ease of separation.","authors":"R Bhatt, B Scott, S Whitney, R N Bryan, L Cloney, A Lebedev","doi":"10.1080/07328319908044696","DOIUrl":"https://doi.org/10.1080/07328319908044696","url":null,"abstract":"Cycling Probe Technology (CPT) is a signal amplification system that allows detection of nucleic acid target sequences without target amplification. CPT employs a sequence specific chimeric probe, typically DNA-RNA-DNA, which hybridizes to a complementary target DNA sequence and becomes a substrate for RNase H. Cleavage occurs at the RNA internucleotide linkages and results in dissociation of the probe from the target, thereby making it available for the next probe molecule. This communication describes the use of oligonucleotides attached to solid supports for target capture and release followed by solution and solid phase cycling. Through the attachment of chimeric probes to Sera-Mag magnetic particles (SMP) a simple and effective method of separating the cleaved probe from non-cycled probe has been developed. By capturing the target DNA on particles and separating it from the extraneous non-specific DNA we are able to dramatically reduce background and thus discriminate between samples of Methicillin Resistant (MRSA) and Methicillin Sensitive (MSSA) Staphylococcus Aureus. We conjugated oligonucleotide probes to SMPs (approximately 1 um) and Nylon beads (NB) which were coated with ID Biomedical's proprietary coating materials (R, patent pending). The general structure of the constructs is shown below: [table: see text]","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1297-9"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044696","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

MMI linkage modification increases potency and stability of H-ras antisense oligonucleotides. MMI链修饰提高了H-ras反义寡核苷酸的效价和稳定性。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044723

L M Cowsert, C T Ohashi, B Bhat, D Peoc'h, A M Symons, P D Cook, M Manoharan

引用次数: 1

Cytotoxicity and in-vivo tolerance of FdUMP[10]: a novel pro-drug of the TS inhibitory nucleotide FdUMP. FdUMP的细胞毒性和体内耐受性[10]:一种新的TS抑制核苷酸FdUMP前药。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044836

W H Gmeiner, A Skradis, R T Pon, J Liu

引用次数: 12

A 38 kDa nuclear protein is involved in the retention of an antisense oligonucleotide directed against cytosolic phospholipase A2. 一个38kda的核蛋白参与了针对胞质磷脂酶A2的反义寡核苷酸的保留。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044819

C Griffoni, E Spisni, M Orlandi, S Santi, M Riccio, V Tomasi

引用次数: 5

Antisense oligodeoxynucleotide complementary to CXCR4 mRNA block replication of HIV-1 in COS cells. 与CXCR4 mRNA互补的反义寡脱氧核苷酸可阻断COS细胞中HIV-1的复制。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044828

A Kusunoki, A Wada, N Kurosaki, T Kimura, K Takai, N Yamamoto, H Takaku

{"title":"Antisense oligodeoxynucleotide complementary to CXCR4 mRNA block replication of HIV-1 in COS cells.","authors":"A Kusunoki, A Wada, N Kurosaki, T Kimura, K Takai, N Yamamoto, H Takaku","doi":"10.1080/07328319908044828","DOIUrl":"https://doi.org/10.1080/07328319908044828","url":null,"abstract":"CXCR4 is both a chemokine receptor and an entry co-receptor for the T-cell line-adapted human immunodeficiency virus type 1 (HIV-1). To find a more efficacious therapeutic treatment of acquired immunodeficiency syndrome, we examined the effects of antisense oligonucleotides on CXCR4 production. COS cells, stably expressing CXCR4 and CD4, were incubated with several kinds of oligonucleotides. Total human p24 antigen production was determined using an enzyme-linked immunosorbent assay system. An antisense phosphorothioate-modified oligonucleotide, complementary to the translation initiation region of the CXCR4 mRNA, showed minimal inhibition of p24 antigen production at the high concentration of 2 microM. On the other hand, the antisense phosphorothioate oligonucleotide, when used with transfection reagents, showed high efficiency at low concentrations, and confirmed the sequence-specific action. Interestingly, the oligonucleotide with the natural phosphodiester backbone, when used with the transfection reagents, also had high functional effects, comparable to the modified oligonucleotide. This study defines the prerequisite criteria necessary for the design and the application of antisense oligonucleotides against HIV-1 in vivo.","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1705-8"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044828","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Enzymatic hydrolysis and biological activity of oligonucleotides containing 5-substituted pyrimidine bases. 含5-取代嘧啶基寡核苷酸的酶解和生物活性。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044816

L Otvös, J Sági, G Sági, A Szemzõ, F D Tóth, A Jeney

引用次数: 6

Thermodynamics of site-specific variant tRNA(Ala) acceptor stem microhairpins. 位点特异性变异tRNA(Ala)受体茎微发夹的热力学。

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044788

E Biala, W McClain, P Strazewski

引用次数: 1

Synthesis of new photocross-linking 5-C-base-substituted UTP analogs and their application in highly selective affinity labelling of the tick-borne encephalitis virus RNA replicase proteins. 新的光交联5- c碱基取代UTP类似物的合成及其在蜱传脑炎病毒RNA复制酶蛋白高选择性亲和标记中的应用

Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044771

O V Morozova, D M Kolpashchikov, T M Ivanova, T S Godovikova

引用次数: 1