J A McCloskey, A B Whitehill, J Rozenski, F Qiu, P F Crain
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New techniques for the rapid characterization of oligonucleotides by mass spectrometry.
Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.
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