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Nucleosides & nucleotides最新文献

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Protein and RNA of human telomerase as targets for modified oligonucleotides. 人类端粒酶的蛋白和RNA作为修饰寡核苷酸的靶标。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044832
M von Janta-Lipinski, K Gaertner, C Lehmann, H Scheer, J Schildt, E Matthes
{"title":"Protein and RNA of human telomerase as targets for modified oligonucleotides.","authors":"M von Janta-Lipinski,&nbsp;K Gaertner,&nbsp;C Lehmann,&nbsp;H Scheer,&nbsp;J Schildt,&nbsp;E Matthes","doi":"10.1080/07328319908044832","DOIUrl":"https://doi.org/10.1080/07328319908044832","url":null,"abstract":"<p><p>Chimeric oligodeoxynucleotides containing phosphorothioate and N3'-->P5' phosphoramidate linkages were synthesized. These oligomers show a high inhibitory activity against human telomerase.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1719-20"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cytarabine-induced destabilization and bending of a model Okazaki fragment. 阿糖胞苷诱导的模型冈崎片段的不稳定和弯曲。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044789
W H Gmeiner, A Skradis, R T Pon, J Liu
{"title":"Cytarabine-induced destabilization and bending of a model Okazaki fragment.","authors":"W H Gmeiner,&nbsp;A Skradis,&nbsp;R T Pon,&nbsp;J Liu","doi":"10.1080/07328319908044789","DOIUrl":"https://doi.org/10.1080/07328319908044789","url":null,"abstract":"<p><p>The effects of cytarabine on the structural and thermodynamic properties of an Okazaki fragment were investigated using UV hyperchromicity and 2D 1H NMR. Cytarabine significantly decreased the stability of this model Okazaki fragment, decreasing the melting temperature from 46.8 degrees C to 42.4 degrees C at 1.33 x 10(-5) M. Cytarabine also markedly increased the bend angle of the Okazaki fragment duplex from 20 degrees to 42 degrees. Changes to the structures and stabilities of Okazaki fragments may cause the biological effects of cytarabine.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1577-8"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044789","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The 9-fluorenylmethoxycarbonyl (Fmoc) group and its use in oligonucleotide synthesis. 9-芴基甲氧羰基(Fmoc)基团及其在寡核苷酸合成中的应用。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044667
H Schirmeister-Tichy, G G Alvarado, W Pfleiderer
{"title":"The 9-fluorenylmethoxycarbonyl (Fmoc) group and its use in oligonucleotide synthesis.","authors":"H Schirmeister-Tichy,&nbsp;G G Alvarado,&nbsp;W Pfleiderer","doi":"10.1080/07328319908044667","DOIUrl":"https://doi.org/10.1080/07328319908044667","url":null,"abstract":"<p><p>The introduction of the base-labile 9-fluorenylmethoxycarbonyl (Fmoc) group into the exocyclic amino function of 2'-deoxynucleosides and their dimethoxytritylation and phosphitylation is described. The resulting key intermediates were investigated in the built-up of different oligodeoxyribonucleoside phosphate and thiophosphate chains which were deprotected under mild basic conditions leading to crude oligomers of high purity.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1219-20"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044667","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Antisense effects of oligonucleotides complementary to the hairpin of the Leishmania mini-exon RNA. 利什曼原虫迷你外显子RNA发夹互补寡核苷酸的反义作用。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044827
D Compagno, J J Toulmé
{"title":"Antisense effects of oligonucleotides complementary to the hairpin of the Leishmania mini-exon RNA.","authors":"D Compagno,&nbsp;J J Toulmé","doi":"10.1080/07328319908044827","DOIUrl":"https://doi.org/10.1080/07328319908044827","url":null,"abstract":"<p><p>We investigated the binding and the translation inhibitory properties of hexadecamers complementary to the mini-exon sequence of the protozoan parasite Leishmania amazonensis. This targeted RNA region folds into a hairpin. Large differences were observed in the antisense properties of the different oligomers although their binding to RNA always requires the disruption of the stem region.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1701-4"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Boranophosphate nucleic acids--a versatile DNA backbone. 硼磷酸核酸——一种多功能的DNA骨架。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044721
V Rait, D Sergueev, J Summers, K He, F Huang, B Krzyzanowska, B R Shaw
{"title":"Boranophosphate nucleic acids--a versatile DNA backbone.","authors":"V Rait,&nbsp;D Sergueev,&nbsp;J Summers,&nbsp;K He,&nbsp;F Huang,&nbsp;B Krzyzanowska,&nbsp;B R Shaw","doi":"10.1080/07328319908044721","DOIUrl":"https://doi.org/10.1080/07328319908044721","url":null,"abstract":"<p><p>Important chemical and biochemical properties of boranophosphate DNA and RNA oligonucleotides are reviewed. Stereoregular boranophosphate oligomers can be synthesized enzymatically and form stable duplexes with DNA. Fully boronated, non-stereoregular oligothymidylates, synthesized chemically, form hybrids with poly(A) that have lower melting points than oligothymidylate:poly(A), yet they nevertheless can support the RNase H mediated cleavage of RNA.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1379-80"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044721","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Binding of chemically-modified oligonucleotides to the double-stranded stem of an RNA hairpin. 化学修饰的寡核苷酸与RNA发夹的双链茎的结合。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044810
K Aupeix, J J Toulmé
{"title":"Binding of chemically-modified oligonucleotides to the double-stranded stem of an RNA hairpin.","authors":"K Aupeix,&nbsp;J J Toulmé","doi":"10.1080/07328319908044810","DOIUrl":"https://doi.org/10.1080/07328319908044810","url":null,"abstract":"<p><p>We monitored the binding of triplex-forming oligopyrimidines to the double-stranded stem of the RNA hairpin responsible for the gag-pol frameshift in HIV-1. Whereas the substitution of 5, propynyl-C for C had a limited effect, the use of a Peptide Nucleic Acid 12mer led to a drastic reduction in the stability of the oligomer/RNA complex.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1647-50"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044810","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Chemical synthesis of oligonucleotides containing the (6-4) photoproduct at the thymine-cytosine site and its repair by (6-4) photolyase. 胸腺嘧啶-胞嘧啶位点上含有(6-4)光产物的寡核苷酸的化学合成及其(6-4)光解酶的修复。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044706
S Iwai, T Mizukoshi, K Hitomi, T Todo
{"title":"Chemical synthesis of oligonucleotides containing the (6-4) photoproduct at the thymine-cytosine site and its repair by (6-4) photolyase.","authors":"S Iwai,&nbsp;T Mizukoshi,&nbsp;K Hitomi,&nbsp;T Todo","doi":"10.1080/07328319908044706","DOIUrl":"https://doi.org/10.1080/07328319908044706","url":null,"abstract":"<p><p>A phosphoramidite building block of the T(6-4)C photoproduct was synthesized. One of the differences from T(6-4)T was formation of cytosine hydrates by UV irradiation, and the other was acylation of the amino function with the capping reagent. The capping step was omitted to improve the yield of the desired oligonucleotides. Characterization of the (6-4) photolyase using one of the oligonucleotides revealed that this enzyme restores the pyrimidines in T(6-4)C to their original structures.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1325-7"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
New techniques for the rapid characterization of oligonucleotides by mass spectrometry. 质谱法快速表征寡核苷酸的新技术。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044782
J A McCloskey, A B Whitehill, J Rozenski, F Qiu, P F Crain
{"title":"New techniques for the rapid characterization of oligonucleotides by mass spectrometry.","authors":"J A McCloskey,&nbsp;A B Whitehill,&nbsp;J Rozenski,&nbsp;F Qiu,&nbsp;P F Crain","doi":"10.1080/07328319908044782","DOIUrl":"https://doi.org/10.1080/07328319908044782","url":null,"abstract":"<p><p>Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1549-53"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
2-5A-PNA complexes: a novel class of antisense compounds. 2-5A-PNA复合物:一类新的反义化合物。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044761
J C Verheijen, S F Bayly, M R Player, P F Torrence, G A van der Marel, J H van Boom
{"title":"2-5A-PNA complexes: a novel class of antisense compounds.","authors":"J C Verheijen,&nbsp;S F Bayly,&nbsp;M R Player,&nbsp;P F Torrence,&nbsp;G A van der Marel,&nbsp;J H van Boom","doi":"10.1080/07328319908044761","DOIUrl":"https://doi.org/10.1080/07328319908044761","url":null,"abstract":"<p><p>This paper presents the fully automated solid phase synthesis of 2-5A-PNA hybrids. These stable antisense probes cause RNase L mediated hydrolysis of target RNA sequences.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1485-6"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Relative effects of graft copolymer and polyamines on triplex stabilization under physiological conditions. 生理条件下接枝共聚物和多胺对三聚体稳定的相对影响。
Nucleosides & nucleotides Pub Date : 1999-06-01 DOI: 10.1080/07328319908044811
A Ferdous, H Watanabe, T Akaike, A Maruyama
{"title":"Relative effects of graft copolymer and polyamines on triplex stabilization under physiological conditions.","authors":"A Ferdous,&nbsp;H Watanabe,&nbsp;T Akaike,&nbsp;A Maruyama","doi":"10.1080/07328319908044811","DOIUrl":"https://doi.org/10.1080/07328319908044811","url":null,"abstract":"<p><p>Triplex-stabilizing effect of a graft copolymer under physiologically relevant conditions has been evaluated and compared with other polyamines. Here we show that the graft copolymer significantly stabilizes triplex DNAs with amazingly higher efficacy than that of physiological concentrations of spermine and spermidine.</p>","PeriodicalId":19222,"journal":{"name":"Nucleosides & nucleotides","volume":"18 6-7","pages":"1651-3"},"PeriodicalIF":0.0,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07328319908044811","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21338151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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