O V Morozova, D M Kolpashchikov, T M Ivanova, T S Godovikova
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引用次数: 1
摘要
合成了一种新的光交联5- c -碱取代UTP类似物，其含有4-叠氮基过氟苯甲酰和4-叠氮基苯胺残基。两种黄病毒蛋白NS5和NS3在类似物存在、辐照(λ > 300 nm)和随后的[α - 32p]NTP掺入下进行RNA合成后被标记。
Synthesis of new photocross-linking 5-C-base-substituted UTP analogs and their application in highly selective affinity labelling of the tick-borne encephalitis virus RNA replicase proteins.
A new photocross-linking 5-C-base-substituted UTP analogs, carrying 4-azidoperfluorobenzoyl and 4-azidoaniline residues were synthesized. Two flavivirus proteins NS5 and NS3 are shown to be labelled after RNA synthesis in the presence of the analogs, irradiation (lambda > 300 nm) and subsequent [alpha-32P]NTP incorporation.
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