Neuroscience最新文献_第2页

Spatial and temporal patterns of brain neural activity mediating human thermal sensations 介导人类热感觉的大脑神经活动的空间和时间模式。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-24 DOI: 10.1016/j.neuroscience.2024.11.045

Hironori Watanabe , Satoshi Shibuya , Yuta Masuda , Taisuke Sugi , Kiyoshi Saito , Kei Nagashima

{"title":"Spatial and temporal patterns of brain neural activity mediating human thermal sensations","authors":"Hironori Watanabe , Satoshi Shibuya , Yuta Masuda , Taisuke Sugi , Kiyoshi Saito , Kei Nagashima","doi":"10.1016/j.neuroscience.2024.11.045","DOIUrl":"10.1016/j.neuroscience.2024.11.045","url":null,"abstract":"<div><div>This study aimed to elucidate the spatial and temporal patterns of brain neural activity that are associated with cold and hot sensations. Participants (n = 20) sat in a controlled room with their eyes closed and received local thermal stimuli to the right fingers using a Peltier apparatus. The thermal stimuli were repeated 40 times using a paired-thermal stimulus paradigm, comprising a 15 s-reference stimulus (32 °C), followed by 10 s-conditioned stimuli (24 °C and 40 °C, cold and hot conditions, respectively), for which 15-channel electroencephalography (EEG) signals were continuously monitored. To identify the patterns of brain neural activity, an independent component (IC) analysis was applied to the preprocessed EEG data. The equivalent current dipole locations were estimated, followed by clustering of the ICs with a dipole residual variance of <15 %. Subsequently, event-related spectral perturbations were analyzed in each identified cluster to calculate the power changes across specific frequency ranges. The right precentral gyrus, precuneus, medial frontal gyrus, middle frontal gyrus, superior frontal gyrus, cuneus, cingulate gyrus, left precentral gyrus, middle occipital gyrus, and cingulate gyrus were activated in both cold and hot conditions. In most activated regions, EEG power temporal changes were observed across the frequency ranges and were different between the two conditions. These results may suggest that cold and hot sensations are processed through different temporal brain neural activity patterns in overlapping brain regions.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 260-270"},"PeriodicalIF":2.9,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acting with awareness is positively correlated with dorsal anterior cingulate cortex glutamate concentration but both are impaired in Internet gaming disorder 有意识地行动与背侧前扣带回皮层谷氨酸浓度呈正相关，但两者在网络游戏障碍中都会受损。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-24 DOI: 10.1016/j.neuroscience.2024.11.054

Tiantian Hong , Hui Zhou , Wan Xi , Xiumei Li , Yusang Du , Jiaxin Liu , Fengji Geng , Yuzheng Hu

{"title":"Acting with awareness is positively correlated with dorsal anterior cingulate cortex glutamate concentration but both are impaired in Internet gaming disorder","authors":"Tiantian Hong , Hui Zhou , Wan Xi , Xiumei Li , Yusang Du , Jiaxin Liu , Fengji Geng , Yuzheng Hu","doi":"10.1016/j.neuroscience.2024.11.054","DOIUrl":"10.1016/j.neuroscience.2024.11.054","url":null,"abstract":"<div><div>Internet gaming disorder (IGD) is increasingly recognized as a public concern for its adverse impacts on cognition and mental health. In IGD, the transition from goal-directed actions to habitual and eventually compulsive behaviors is accompanied by altered neural response within the dorsal anterior cingulate cortex (dACC), a critical region involved in conscious actions. However, the neurochemical profile of the dACC in IGD and its relationship with behavioral awareness remain poorly understood. In this study, <sup>1</sup>H-magnetic resonance spectroscopy was employed to quantify dACC glutamate concentration and examine its association with the capacity for ‘acting with awareness’ among 21 participants with IGD and 19 recreational game users. Results indicated that dACC glutamate levels and behavioral awareness were significantly lower in the IGD group compared to recreational game users. Moreover, a significant positive correlation between awareness and dACC glutamate concentration emerged in the recreational game users’ group, a relationship attenuated in those with IGD. In an independent cohort of 107 participants, the positive association between awareness and dACC glutamate concentration was replicated. These findings suggest that reduced dACC glutamate in IGD may underlie diminished awareness of maladaptive habitual behaviors. Enhancing dACC neural excitability through neuromodulation or mindfulness training could represent a potential intervention to restore behavioral awareness.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 226-235"},"PeriodicalIF":2.9,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity Matters. 多样性至关重要。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-23 DOI: 10.1016/j.neuroscience.2024.11.057

Francesca Cirulli, Sarah J Spencer, Chen Zhang

引用次数: 0

Neural correlates of empathy in donation decisions: Insights from EEG and machine learning 捐赠决策中移情的神经相关性：脑电图和机器学习的启示

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-23 DOI: 10.1016/j.neuroscience.2024.11.044

Wenhao Mao , Xuejie Shen , Xiaoxu Bai , Ailian Wang

{"title":"Neural correlates of empathy in donation decisions: Insights from EEG and machine learning","authors":"Wenhao Mao , Xuejie Shen , Xiaoxu Bai , Ailian Wang","doi":"10.1016/j.neuroscience.2024.11.044","DOIUrl":"10.1016/j.neuroscience.2024.11.044","url":null,"abstract":"<div><div>Empathy is central to individual and societal well-being. Numerous studies have examined how trait of empathy affects prosocial behavior. However, little studies explored the psychological and neural mechanisms by which different dimensions of trait empathy influence prosocial behavior. Addressing this knowledge gap is important to understand empathy-driven prosocial behavior. We employed an EEG experiment combined with interpretable machine learning methods to probe these questions. We found that empathic concern (EC) played the most pivotal role in donation decision. Behaviorally, EC negatively moderates the effect of perceived closeness and deservedness of charity projects on the willingness to donate. The machine learning results indicate that EC significantly predicts late positive potential (LPP) and beta-band activity during donation information processing. Further regression analysis results indicate that EC, rather than other dimensions of trait empathy, can positively predict LPP amplitude and negatively predict beta-band activity. These results indicated that participants with higher EC scores may experience heightened emotional arousal and the vicarious experience of others’ emotions while processing donation information. Our work adds weight to understanding the relationship between trait empathy and prosocial behavior and provides electrophysiological evidence.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 214-225"},"PeriodicalIF":2.9,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142706112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early changes in intestinal lymphoid and myeloid populations in experimental autoimmune encephalomyelitis 实验性自身免疫性脑脊髓炎肠道淋巴细胞和髓细胞群的早期变化

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-22 DOI: 10.1016/j.neuroscience.2024.11.051

Erwan Baudron , Fabian Docagne , Denis Vivien , Isabelle Bardou

{"title":"Early changes in intestinal lymphoid and myeloid populations in experimental autoimmune encephalomyelitis","authors":"Erwan Baudron , Fabian Docagne , Denis Vivien , Isabelle Bardou","doi":"10.1016/j.neuroscience.2024.11.051","DOIUrl":"10.1016/j.neuroscience.2024.11.051","url":null,"abstract":"<div><div>Intestinal immunity is associated with several autoimmune diseases, such as systemic lupus erythematosus, rheumatoid arthritis, and type 1 diabetes. Recent evidence also suggests its implication in the pathogenesis of autoimmune diseases affecting the central nervous system, such as multiple sclerosis (MS). However, there is ongoing debate regarding which part of the intestinal tract contributes to the development of MS. Therefore, our study aimed to explore the early changes in lymphoid and myeloid immune cells populations in experimental autoimmune encephalomyelitis (EAE), an animal model of MS. We also sought to determine the roles of the colon and/or small intestine in the pathogenesis of EAE.</div><div>By using flow cytometry, we revealed a transient increase in T and B lymphocytes in the ileal lamina propria of EAE mice just before the onset of motor symptoms. Additionally, we highlighted an increase in dendritic cells and monocytes/macrophages in the colonic lamina propria of EAE animals during the presymptomatic phase.</div><div>Altogether, our findings indicate that both small intestine and colon are involved in the pathogenesis of EAE, despite engaging distinct immunological processes. This study provides new insights for understanding the roles of intestinal lymphoid and myeloid immune cells on the pathogenesis of MS and other autoimmune diseases.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 236-242"},"PeriodicalIF":2.9,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreases in metabolic ATP open KATP channels and reduce firing in an auditory brainstem neuron: A dynamic mechanism of firing control during intense activity 代谢 ATP 的减少打开了 KATP 通道并降低了听觉脑干神经元的发射：强烈活动期间发射控制的动态机制。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.052

Daniela Vanessa F. de Siqueira, Natalia Boaretto, Ricardo Maurício Leão

{"title":"Decreases in metabolic ATP open KATP channels and reduce firing in an auditory brainstem neuron: A dynamic mechanism of firing control during intense activity","authors":"Daniela Vanessa F. de Siqueira, Natalia Boaretto, Ricardo Maurício Leão","doi":"10.1016/j.neuroscience.2024.11.052","DOIUrl":"10.1016/j.neuroscience.2024.11.052","url":null,"abstract":"<div><div>Cartwheel (CW) neurons are glycinergic interneurons in the dorsal cochlear nucleus (DCN) that exhibit spontaneous firing, resulting in potent tonic inhibition of fusiform neurons. CW neurons expressing open ATP-sensitive potassium (K<sub>ATP</sub>) channels do not fire spontaneously, and activation of K<sub>ATP</sub> channels halts spontaneous firing in these neurons. However, the conditions that regulate K<sub>ATP</sub> channel opening in CW neurons remain unknown. Here, we tested the hypothesis that fluctuations in metabolic ATP levels modulate K<sub>ATP</sub> channels in CW neurons. Using whole-cell patch-clamp recordings in CW neurons from young rat brain slices (p17-22) with an ATP-free internal solution, we observed that the mitochondrial uncoupler CCCP hyperpolarized the membrane potential, reduced spontaneous firing, and generated an outward current, which was inhibited by the K<sub>ATP</sub> channel antagonist tolbutamide. Additionally, a glucose-free external solution quickly activated K<sub>ATP</sub> channels and ceased spontaneous firing. We hypothesized that intense membrane ion ATPase activity during strong depolarization would deplete intracellular ATP, leading to K<sub>ATP</sub> channel opening. Consistent with this, depolarizing CW neurons with a 250 pA DC did not increase spontaneous firing because the depolarization activated K<sub>ATP</sub> channels; however, the same depolarization after tolbutamide administration increased firing, suggesting that ATP depletion triggered K<sub>ATP</sub> channel opening to limit action potential firing. These results indicate that K<sub>ATP</sub> channels in the DCN provide dynamic control over action potential firing, preventing excessive excitation during high-firing activity.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 171-178"},"PeriodicalIF":2.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impacts of maternal separation stress on ethanol intake and endocannabinoid system in adolescent mice. 母体分离应激对青春期小鼠乙醇摄入量和内源性大麻素系统的影响

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.037

C A Favoretto, N B Bertagna, A Anjos-Santos, C M Loss, B T Rodolpho, T Righi, F R Bezerra, P C Bianchi, F C Cruz

{"title":"Impacts of maternal separation stress on ethanol intake and endocannabinoid system in adolescent mice.","authors":"C A Favoretto, N B Bertagna, A Anjos-Santos, C M Loss, B T Rodolpho, T Righi, F R Bezerra, P C Bianchi, F C Cruz","doi":"10.1016/j.neuroscience.2024.11.037","DOIUrl":"https://doi.org/10.1016/j.neuroscience.2024.11.037","url":null,"abstract":"<p><p>Clinical and preclinical studies suggest that early-life stress can increase the risk of developing ethanol use disorder later in life. Although the endocannabinoid (eCB) system plays a role in stress-related behaviors and ethanol consumption, it remains unclear whether the eCB system is affected in response to a combination of both factors. By using male and female adolescent C57BL/6J mice subjected to a maternal separation (MS) stress paradigm from postnatal day (PND) 1 to 14, we explored (1) the consequences of early-life stress experiences on ethanol consumption in adolescent mice and (2) how these events affect the eCB system and neuronal activation in brain regions associated with the reward system. In Experiment 1, we found that MS increased involuntary ethanol consumption specifically during the first exposure to the drug (during a 24 h-long trial on PND 28) and decreased the active/inactive nose poke ratio (discrimination index) specifically when mice were subjected to 1 h-sessions (PND 82-86) in an operant ethanol self-administration paradigm. In Experiment 2, during a two-bottle free choice paradigm, we found that MS increased mice preference for high ethanol concentrations (15 % and 20 %) but not lower ethanol concentrations (5 % and 10 %). Except for Mgll gene expression in the dorsal striatum (DS) in Experiment 2, no statistically significant effects of MS were observed regarding neuronal activation on the prefrontal cortex, DS, globus pallidus, and substantia nigra following a binge operant ethanol self-administration session (Experiment 1) or the eCB system molecules (Cnr1 and Faah gene expression) in the DS (Experiment 2).</p>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microglial upregulation of CD109 expression in spinal cord of amyotrophic lateral sclerosis mouse model and its role in modulating inflammation and TGFβ/SMAD pathway 肌萎缩性脊髓侧索硬化症小鼠脊髓小胶质细胞CD109表达上调及其在调节炎症和TGFβ/SMAD通路中的作用

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.053

Zhongzhong Li , Yingzhen Zhang , Dongxiao Li , Xinyan Du , Lin Chen , Yansu Guo

{"title":"Microglial upregulation of CD109 expression in spinal cord of amyotrophic lateral sclerosis mouse model and its role in modulating inflammation and TGFβ/SMAD pathway","authors":"Zhongzhong Li , Yingzhen Zhang , Dongxiao Li , Xinyan Du , Lin Chen , Yansu Guo","doi":"10.1016/j.neuroscience.2024.11.053","DOIUrl":"10.1016/j.neuroscience.2024.11.053","url":null,"abstract":"<div><div>CD109 is a multifunctional coreceptor, whose function has been widely studied in tumor progression and metastasis. One of the reported primary roles of CD109 involves down-regulating TGFβ signaling. However, the role of CD109 in central nervous system, especially neurodegenerative disease, is barely known. Here, we examined the expression changes and cellular location of CD109 and TGFβ/SMAD pathway molecules in lumbar spinal cord of SOD1-G93A mice, and explored the role and mechanism of CD109 on LPS-treated BV2 microglia and primary microglia derived from SOD1-G93A mice. Our results showed an increased expression of CD109 and TGFβ/SMAD pathway molecules in lumbar spinal cord of SOD1-G93A mice. Further cellular localization analysis demonstrated that proliferating microglia contributed mainly to the upregulation of CD109 and TGFβ1. Moreover, CD109 intervention in vitro partially reduced inflammatory response and TGFβ/SMAD pathway activation in both LPS-treated BV2 microglia and primary SOD1-G93A microglia. Thus, CD109 was involved in pathogenesis of ALS mice, and interventions targeting on CD109 modulation could be a potential therapeutic strategy for ALS.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 202-213"},"PeriodicalIF":2.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aberrant intra-network resting-state functional connectivity in chronic insomnia with or without cognitive impairment. 伴有或不伴有认知障碍的慢性失眠症患者网络内静息态功能连接异常。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-21 DOI: 10.1016/j.neuroscience.2024.11.046

Jurong Ding, Mengjie Yu, Lihong Li, Mei Yang, Pan Yang, Bo Hua, Xin Ding

{"title":"Aberrant intra-network resting-state functional connectivity in chronic insomnia with or without cognitive impairment.","authors":"Jurong Ding, Mengjie Yu, Lihong Li, Mei Yang, Pan Yang, Bo Hua, Xin Ding","doi":"10.1016/j.neuroscience.2024.11.046","DOIUrl":"https://doi.org/10.1016/j.neuroscience.2024.11.046","url":null,"abstract":"<p><p>Chronic insomnia (CI) is a common sleep disorder in middle-aged and elderly individuals. Long-term sleep deprivation can lead to physical, mental, and cognitive damage. Resting-state networks (RSNs) in the brain are closely linked to cognition and behavior. Therefore, we investigated changes in RSNs to explore behavioral and cognitive abnormalities in middle-aged and elderly CI patients. Resting state functional magnetic resonance imaging (rs-fMRI) and independent component analysis were used to study the intrinsic functional connectivity (FC) of the RSNs in 36 CI patients (20 CI with cognitive impairment (CI-I) patients and 16 CI without cognitive impairment (CI-N) patients) and 20 healthy controls (HC). Two-sample t-tests were used to compare RSNs differences between CI and HC groups and the RSNs differences between CI-I and CI-N groups. Partial correlation analysis was used to explore the relationship between the significant abnormal brain regions in RSN and clinical scales. Compared with HCs, CI patients showed significant differences in multiple RSNs, and FC values in two brain regions within RSNs were correlated with clinical scales. Furthermore, compared with CI-N group, CI-I group also showed significantly altered FC in multiple RSNs. Moreover, FC values in the right middle frontal gyrus within right frontal parietal network of CI-I patients were negatively correlated with the Mini-Mental State Examination scores. These results may explain hyperarousal, decreased attention and motor function impairments in CI patients. Furthermore, the aberrant alterations of RSNs in CI-I patients may play a crucial role in the onset and progression of cognitive impairment in CI patients.</p>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiated and mature neurons are more responsive to neurotoxicant exposure at both transcriptional and translational levels 分化和成熟的神经元在转录和翻译水平上对暴露于神经毒素的反应更为敏感。

IF 2.9 3区医学

Neuroscience Pub Date : 2024-11-20 DOI: 10.1016/j.neuroscience.2024.11.017

Sana Sarkar , Anuj Pandey , Sanjeev Kumar Yadav , Mohammed Haris Siddiqui , A.B. Pant , Sanjay Yadav

{"title":"Differentiated and mature neurons are more responsive to neurotoxicant exposure at both transcriptional and translational levels","authors":"Sana Sarkar , Anuj Pandey , Sanjeev Kumar Yadav , Mohammed Haris Siddiqui , A.B. Pant , Sanjay Yadav","doi":"10.1016/j.neuroscience.2024.11.017","DOIUrl":"10.1016/j.neuroscience.2024.11.017","url":null,"abstract":"<div><div>SH-SY5Y human neuroblastoma cells have been extensively used as an <em>in vitro</em> model system in a diverse range of studies involving neurodevelopment, neurotoxicity, neurodegeneration, and neuronal ageing. Both naïve and differentiated phenotypes of SH-SY5Y cells are utilized to model human neurons under <em>in vitro</em> conditions. The process of differentiation causes extensive remodeling of neuronal cells at multiple omic levels, including the epigenome and proteome. In the present investigation, the miRNAome and proteome profiles of arsenic-treated naïve and differentiated SH-SY5Y cells were generated using the miRNA OpenArray technology and high-resolution mass spectrometry. Our findings demonstrated that differentiation dramatically affected the response of SH-SY5Y cells to toxicant exposure, as indicated by increased tolerance of differentiated cells against arsenic exposure compared to naïve cells in cell viability assay. Arsenic-exposed naïve and differentiated SH-SY5Y cells possess distinct miRNA and protein profiles with few similarities. Compared to naïve cells, differentiated cells have undergone higher deregulation in the expression of brain-enriched miRNAs and proteins and have shown a more drastic decrease in oxygen consumption rate, which is a measure of mitochondrial respiration after exposure to arsenic. Proteins identified in arsenic-treated differentiated SH-SY5Y cells were more enriched in pathways underlying multifactorial neurotoxic events. Additionally, more functional regulatory modules have been identified between the miRNAs and proteins differentially expressed in arsenic-treated differentiated SH-SY5Y cells relative to naïve cells. Collectively, our studies have shown that differentiated SH-SY5Y cells displayed alterations in the expression of a greater number of miRNAs and proteins following neurotoxicant exposure, indicating their higher responsivity.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"564 ","pages":"Pages 110-125"},"PeriodicalIF":2.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0