Neuroscience最新文献_第2页

Neuroproteomics applied to the study of visual cortex plasticity 神经蛋白质组学在视觉皮层可塑性研究中的应用

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-19 DOI: 10.1016/j.neuroscience.2025.04.021

Francesco Mattia Rossi , Tommaso Pizzorusso

{"title":"Neuroproteomics applied to the study of visual cortex plasticity","authors":"Francesco Mattia Rossi , Tommaso Pizzorusso","doi":"10.1016/j.neuroscience.2025.04.021","DOIUrl":"10.1016/j.neuroscience.2025.04.021","url":null,"abstract":"<div><div>The huge complexity of neuronal circuits arises from a temporarily overlapped influence of genetic and environmental factors (Nature and Nurture). During specific temporal windows of postnatal development, the so-called critical or sensitive periods of plasticity, the brain is particularly susceptible to the effects of experience, though this sensitivity declines with age. The most widely used experimental paradigm for studying critical periods of plasticity is the ocular dominance model in the mammalian visual cortex. Recent advancements in large-scale methodological approaches have enabled the analysis of the cellular and molecular factors regulating plasticity, highlighting the complex interaction among various metabolic and regulatory pathways. Traditionally, genomic and transcriptomic techniques have been employed to investigate the Central Nervous System in a comprehensive manner, including studies on critical period plasticity in the visual cortex. However, it is the technical advancements in proteomic approaches that have established neuroproteomics as a powerful tool for investigating both normal and pathological brain states. Despite its potential, proteomics has been underutilized in studying visual cortical plasticity. Here, we review existing studies and emphasize the importance of exploiting neuroproteomics, and of integrating with other complementary “omic” approaches, to accurately identify the true active cellular agents and ultimate mediators of brain functions.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"576 ","pages":"Pages 8-16"},"PeriodicalIF":2.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Motor learning is regulated by postnatal GDNF levels in Purkinje cells 运动学习是由出生后浦肯野细胞中的GDNF水平调节的

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-18 DOI: 10.1016/j.neuroscience.2025.04.030

Elina Nagaeva , Giorgio Turconi , Kärt Mätlik , Mikael Segerstråle , Soophie Olfat , Vilma Iivanainen , Tomi Taira , Jaan-Olle Andressoo

{"title":"Motor learning is regulated by postnatal GDNF levels in Purkinje cells","authors":"Elina Nagaeva , Giorgio Turconi , Kärt Mätlik , Mikael Segerstråle , Soophie Olfat , Vilma Iivanainen , Tomi Taira , Jaan-Olle Andressoo","doi":"10.1016/j.neuroscience.2025.04.030","DOIUrl":"10.1016/j.neuroscience.2025.04.030","url":null,"abstract":"<div><div>Purkinje cells (PCs), the sole output neurons of the cerebellar cortex, are crucial for cerebellum-dependent motor learning. In cerebellar ataxia, reduction in motor function and learning associates with decreased spontaneous activity of PCs. Thus, understanding what molecules regulate PCs activity is important.</div><div>Previously, we demonstrated that a ubiquitous 2-fold increase of endogenous glial cell line-derived neurotrophic factor (GDNF) improves motor function in adult mice and motor learning and coordination in aged mice. However, since GDNF impacts many organ systems the underlying mechanism remained elusive. Here we utilize GDNF Hypermorphic, conditional GDNF Hypermorphic and conditional knock-out mouse models to reveal that up to a 2-fold increase in endogenous GDNF, specifically in PCs postnatally, is sufficient to enhance motor learning. We find that improved motor learning associates with increased glutamatergic input to PCs and with elevated spontaneous firing rate of PCs, opposite to cerebellar ataxia where reduction in motor function and learning associates with decreased spontaneous activity of PCs. Analysis of the human cerebellum revealed that normal interindividual variation in GDNF expression levels falls in the same variation range as studied in the mouse models, suggesting that interindividual variation in PC GDNF levels may contribute to interindividual variation in PC function. Collectively, our findings reveal how a relatively small change in postnatal GDNF expression level within the physiological range in one cell type, the PCs, affects motor learning. Thus, drugs enhancing postnatal GDNF expression in PCs or cerebellar GDNF signaling may have potential in treating cerebellar ataxias, making an interesting topic for future studies.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"576 ","pages":"Pages 27-41"},"PeriodicalIF":2.9,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Corrigendum to “Similar pattern of peripheral neuropathy in mouse models of type 1 diabetes and Alzheimer’s disease” [Neuroscience 202 (2012) 405–412] “1型糖尿病和阿尔茨海默病小鼠模型中相似的周围神经病变模式”的勘误表[Neuroscience 202 (2012) 405-412]

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-18 DOI: 10.1016/j.neuroscience.2025.04.017

C.G. Jolivalt, N.A. Calcutt, E. Masliah

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Animal models of epilepsy after ischemic stroke 缺血性脑卒中后癫痫动物模型的建立

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-18 DOI: 10.1016/j.neuroscience.2025.04.029

Min He , Donghui Wang , Kunling Yang, Hengchang Qi, Chaoning Liu, Lanfeng Sun, Lei Wei, Yuan Wu

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Cortical dynamics underlying initiation of rapid steps with contrasting postural demands 在不同姿势要求下，快速步的开始是由皮质动力学引起的

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-17 DOI: 10.1016/j.neuroscience.2025.04.026

Ilse Giesbers , Lucas Billen , Joris van der Cruijsen , Brian D. Corneil , Vivian Weerdesteyn

{"title":"Cortical dynamics underlying initiation of rapid steps with contrasting postural demands","authors":"Ilse Giesbers , Lucas Billen , Joris van der Cruijsen , Brian D. Corneil , Vivian Weerdesteyn","doi":"10.1016/j.neuroscience.2025.04.026","DOIUrl":"10.1016/j.neuroscience.2025.04.026","url":null,"abstract":"<div><div>Our ability to flexibly initiate rapid visually-guided stepping movements can be measured in the form of express visuomotor responses (EVRs), which are short-latency (∼100 ms), goal-directed bursts of lower-limb muscle activity. Interestingly, we previously demonstrated that recruitment of anticipatory postural adjustments (APAs) interacted with the subcortically-generated EVRs in the lower limb, suggesting context-dependent top-down modulation.</div><div>We investigated the associated cortical dynamics prior to and during rapid step initiation towards a salient visual target in twenty-one young, healthy individuals while stepping under varying postural demands. We recorded high-density EEG, surface electromyography from gluteus medius and ground-reaction forces.</div><div>Independent component analysis and time–frequency statistics revealed significant, yet relatively modest differences between conditions in preparatory cortical dynamics, most evidently in primary motor areas. Following target presentation, we observed stronger theta and alpha power enhancement in the supplementary motor area, and stronger alpha and beta power decrease in primary motor, parietal and occipital clusters during APA recruitment that preceded steps under high postural demands. Side-specific changes in motor cortex lagged the timing of EVR expression, supporting the EVR’s purportedly subcortical origin. Together, our findings point towards greater cortical involvement in step initiation under high postural demands as compared to more reflexive, stimulus-driven steps. These findings may be particularly relevant for populations where postural control is impaired by age or disease, as more cortical resources may need to be allocated during stepping.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"575 ","pages":"Pages 104-121"},"PeriodicalIF":2.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Characterizing the phenotype of pre-Bötzinger Complex neurons in rats 大鼠pre-Bötzinger复杂神经元表型的表征

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-17 DOI: 10.1016/j.neuroscience.2025.04.028

Angela A. Connelly , Jaspreet K. Bassi , Joshua J. Voger , Andrew G. Butler , Stuart J. McDougall , Andrew M. Allen , Mariana R. Melo

{"title":"Characterizing the phenotype of pre-Bötzinger Complex neurons in rats","authors":"Angela A. Connelly , Jaspreet K. Bassi , Joshua J. Voger , Andrew G. Butler , Stuart J. McDougall , Andrew M. Allen , Mariana R. Melo","doi":"10.1016/j.neuroscience.2025.04.028","DOIUrl":"10.1016/j.neuroscience.2025.04.028","url":null,"abstract":"<div><div>The pre-Bötzinger Complex (preBötC) is a key medullary region responsible for generating breathing. In rodents, preBötC neurons are divided almost evenly between excitatory neurons, which express vesicular glutamate transporter 2 (VGluT2), and inhibitory neurons expressing either glutamic acid decarboxylase (GAD) or glycine transporter 2 (GlyT2). The interaction between excitatory and inhibitory neurons plays a significant role in rhythmic breathing and its coordination with other physiological functions. However, comprehensive knowledge about the classification and the physiological roles of preBötC neuronal subpopulations in adults is limited. This arises due to the complex interconnections of preBötC with adjacent regions, undefined anatomical boundaries of the region, diverse neurochemical signatures without clear functional distinctions, and the predominant reliance on prenatal mouse data. In this study, we aimed to enhance the understanding of the neurochemical signatures of preBötC neurons and their proportions by rigorously defining the boundaries of the preBötC in adult male rats (n = 3). For this, we employed RNAscope <em>in situ</em> hybridization to identify, and anatomically and systematically characterize, the subgroups of preBötC neurons expressing VGluT2, somatostatin (SST), GAD1, vesicular GABA transporter (VGAT) and/or reelin. We observed that most SST-expressing neurons are glutamatergic and comprise over 50% of the excitatory population of preBötC. In addition, a considerable proportion of SST-expressing neurons express GAD1. Our results also show that approximately half of SST-expressing neurons co-express reelin, and that most reelin-expressing neurons are glutamatergic. A key finding is that the combination of immunohistochemistry for reelin with parvalbumin, is a reliable marker to define the anatomical location of preBötC.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"576 ","pages":"Pages 45-58"},"PeriodicalIF":2.9,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Authoritarianism and the brain: Structural MR correlates associated with polarized left- and right-wing ideology traits 专制主义与大脑：与极化的左翼和右翼意识形态特征相关的磁共振结构相关性

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-16 DOI: 10.1016/j.neuroscience.2025.04.027

Jesús Adrián-Ventura , Diego Avendaño , Anna Miró-Padilla , Anastasia Cherednichenko , César Ávila , Angelo Fasce

{"title":"Authoritarianism and the brain: Structural MR correlates associated with polarized left- and right-wing ideology traits","authors":"Jesús Adrián-Ventura , Diego Avendaño , Anna Miró-Padilla , Anastasia Cherednichenko , César Ávila , Angelo Fasce","doi":"10.1016/j.neuroscience.2025.04.027","DOIUrl":"10.1016/j.neuroscience.2025.04.027","url":null,"abstract":"<div><div>Authoritarian attitudes across the political spectrum foster radical behaviors, which adversely affect the social fabric. Both left-wing (LWA) and right-wing (RWA) forms of authoritarianism have been described in relation to their psychological correlates, yet little is known about their neurobiological basis. In this study, we explored brain structural correlates (e.g., in cortical thickness (CT) and gray matter (GM) volume) of authoritarianism. For this purpose, we assessed authoritarian dispositions in a sample of 100 young adults and collected 3 T MR images. Images were computed using the CAT12 toolbox. Behaviorally, both the LWA and RWA were positively associated with negative urgency; the LWA also showed a robust positive association with trait anxiety. At the neural level, results showed a negative correlation (<em>r</em> = -0.48) between RWA and a GM volume cluster located in the dorsomedial prefrontal cortex (dmPFC). In addition, we also observed a negative correlation (<em>r</em> = -0.41) between the LWA anti-hierarchical aggression subscale and a CT cluster located in the right anterior insula. Additionally, the resulting clusters converged with further left-wing and right-wing ideology scales related to LWA and RWA, thus providing a robustness check. These results are supported by previous studies showing the relevance of the dmPFC and the anterior insula on social cognition and empathy/inhibitory control, respectively.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"575 ","pages":"Pages 95-103"},"PeriodicalIF":2.9,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Distinct mitotic dynamics and neuronal migration patterns between gyri and sulci in the ferret neocortex during cortical folding 雪貂皮层折叠过程中脑回和脑沟间不同的有丝分裂动力学和神经元迁移模式

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-15 DOI: 10.1016/j.neuroscience.2025.04.018

You-Ning Lin , Shi-Yuan Tong , Jun-Wei Cao , Ni Zong , Jun-Yang Chen , Fu-Wei Yang , Chen-Xi Wang , Lin-Yun Liu , Wen-Dong Xu , Yong-Chun Yu

{"title":"Distinct mitotic dynamics and neuronal migration patterns between gyri and sulci in the ferret neocortex during cortical folding","authors":"You-Ning Lin , Shi-Yuan Tong , Jun-Wei Cao , Ni Zong , Jun-Yang Chen , Fu-Wei Yang , Chen-Xi Wang , Lin-Yun Liu , Wen-Dong Xu , Yong-Chun Yu","doi":"10.1016/j.neuroscience.2025.04.018","DOIUrl":"10.1016/j.neuroscience.2025.04.018","url":null,"abstract":"<div><div>Neocortical folding (i.e., gyrification) is a key evolutionary and developmental feature of the brain, facilitating cortical surface expansion and enhanced cognitive function. However, the precise strategies and mechanisms underlying cortical folding remain incompletely understood. In this study, we systematically investigated the dynamic formation of neocortical folding in the ferret. Our findings reveal significant differences in neurogenesis and neuronal migration between the developing lateral gyrus (LG) and adjacent lateral sulcus (LS) of the ferret neocortex. Specifically, progenitors in the LG exhibited higher mitosis activity and a shorter S-phase duration compared to those in the LS. Additionally, immature neurons in the LG followed a fan-like migration pattern, whereas those in the LS exhibited a flower bud-like pattern. Organotypic slice cultures and time-lapse imaging further demonstrated that the migration trajectory of immature neurons to the neocortex is more straightforward in the LG than in the LS. Together, these results highlight distinct cellular behaviors between the developing gyrus and sulcus, providing novel insights into cellular mechanisms underlying cortex folding.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"576 ","pages":"Pages 69-79"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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High-frequency repetitive transcranial magnetic stimulation attenuates white matter damage and improves functional recovery in rats with ischemic stroke 高频重复经颅磁刺激可减轻缺血性脑卒中大鼠脑白质损伤，促进脑功能恢复

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-14 DOI: 10.1016/j.neuroscience.2025.04.024

Can Luo , Xiangyu Tang , Haoyue Shao , Feng Guo

{"title":"High-frequency repetitive transcranial magnetic stimulation attenuates white matter damage and improves functional recovery in rats with ischemic stroke","authors":"Can Luo , Xiangyu Tang , Haoyue Shao , Feng Guo","doi":"10.1016/j.neuroscience.2025.04.024","DOIUrl":"10.1016/j.neuroscience.2025.04.024","url":null,"abstract":"<div><div>Stroke is a major cause of acquired disability and the second most frequent cause of dementia, while specific therapeutic rehabilitation strategies remain limited. Repetitive transcranial magnetic stimulation(rTMS) is a well-known rehabilitation modality after cerebral ischemic injury. White matter damage is an important contributor to motor and cognitive dysfunctions after stroke. This study aimed to evaluate the effect of rTMS on white matter recovery and neurological deficits in ischemic stroke. Grip strength test and novel object recognition test were conducted to assess motor and cognitive functions after middle cerebral artery occlusion(MCAO). MRI, including Diffusion tensor imaging (DTI) and Diffusion Tensor Tractography (DTT) were performed to evaluate white matter injury in MCAO rats. Moreover, Western blotting were detected to observe related myelin damage proteins in the ischemic brain. The results revealed that 10 Hz rTMS alleviated the motor and cognitive deficits in rats after ischemic surgery. Besides, the data from DTI and DTT showing that 10 Hz rTMS ameliorated the white matter lesion of rats after cerebral ischemia. In addition, 10 Hz rTMS attenuated significant loss of the myelin sheath by enhanced myelin associated proteins levels in the ischemic brain of ischemic rats. These findings suggest that 10 Hz rTMS exerted therapeutic neuroprotective properties after ischemic stroke, in a manner that may be associated with enhancing structural repairment of the white matter, which may provide a potential therapeutic strategy for ischemic stroke.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"575 ","pages":"Pages 48-56"},"PeriodicalIF":2.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Diabetes affects AD through plasma Aβ40: A Mendelian randomization study 糖尿病通过血浆Aβ40影响AD：一项孟德尔随机研究

IF 2.9 3区医学

Neuroscience Pub Date : 2025-04-13 DOI: 10.1016/j.neuroscience.2025.04.025

Qiumin Yang , Delong Huang , Zhaojing Zhang , Haiyan Gao , Junhao Wu , Haoshu Zhong , Xiaowei Guo , Yiren Wang , Hemu Zhou , Chengzhen Liu , Xiaodong Duan

{"title":"Diabetes affects AD through plasma Aβ40: A Mendelian randomization study","authors":"Qiumin Yang , Delong Huang , Zhaojing Zhang , Haiyan Gao , Junhao Wu , Haoshu Zhong , Xiaowei Guo , Yiren Wang , Hemu Zhou , Chengzhen Liu , Xiaodong Duan","doi":"10.1016/j.neuroscience.2025.04.025","DOIUrl":"10.1016/j.neuroscience.2025.04.025","url":null,"abstract":"<div><div>Amyloid and tau proteins are important proteins in the pathological changes of Alzheimer’s disease (AD), while Aβ pathology and tau pathology are the most critical factors contributing to the development of AD. Some studies have shown that there is a causal relationship between AD and diabetes mellitus, but there are no studies showing a causal relationship between diabetic traits and AD biomarkers, so further exploration is needed. We first summarized and analyzed the currently published literature on the link between diabetes and AD through a systematic review. Forest plots were used to observe whether there is an association between diabetes and AD. Then a two-sample Mendelian randomization (MR) analysis based on GWAS summary statistics was performed to verify the causal relationship between diabetic traits and AD biomarkers. Based on summary statistics from the GWAS, potential causal relationships between diabetic traits and AD biomarkers were explored separately. The results of the <em>meta</em>-analysis part showed that diabetes can increase the risk of AD. Meanwhile, our two-sample MR results showed a significant causal relationship between diabetes and plasma Aβ40. In addition, our two-sample MR results also showed a causal relationship between increased HbA1c and plasma APLP2. Other diabetic traits may have potential effects on different AD plasma markers.</div></div>","PeriodicalId":19142,"journal":{"name":"Neuroscience","volume":"575 ","pages":"Pages 131-139"},"PeriodicalIF":2.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0