Neurological Research最新文献_第2页

miR-363-5p protects from neuropathic pain in chronic constriction injury (CCI) rat models and regulates Schwann cell injury via negatively modulating SERPING1. miR-363-5p在慢性收缩损伤（CCI）大鼠模型中保护神经性疼痛，并通过负调节SERPING1调节雪旺细胞损伤。

IF 1.7 4区医学

Neurological Research Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1080/01616412.2024.2438613

Huihui Wu, Liang Zhu, Xia Geng, Xiaona Guo, Tingting Wang, Jingjing Xu, Linkai Jiang, Weibo Zhang

{"title":"miR-363-5p protects from neuropathic pain in chronic constriction injury (CCI) rat models and regulates Schwann cell injury via negatively modulating SERPING1.","authors":"Huihui Wu, Liang Zhu, Xia Geng, Xiaona Guo, Tingting Wang, Jingjing Xu, Linkai Jiang, Weibo Zhang","doi":"10.1080/01616412.2024.2438613","DOIUrl":"10.1080/01616412.2024.2438613","url":null,"abstract":"Objectives: Due to the complex and unclear pathogenesis of neuropathic pain, there is a lack of effective therapeutic strategy. miR-363-5p was considered of great potential in mediating the development of neuropathic pain, which has not been confirmed with direct evidence. This study evaluated the role of miR-363-5p in neuropathic pain with animal and cell models, aiming to reveal the potential of miR-363-5p in target therapy of neuropathic pain.Methods: Chronic constriction injury (CCI) rat models were established as the neuropathic pain model. The expression of miR-363-5p and its target was evaluated by PCR. The painology behaviors were evaluated to assess the function of miR-363-5p. Schwann cells were induced with LPS mimicking cell injury during neuropathic pain. Inflammation and cell growth were estimated by ELISA and CCK8 assays.Results: Significant downregulation of miR-363-5p and upregulation of SERPING1 were observed in CCI rats. miR-363-5p negatively regulated SERPING1 in CCI rats and LPS-induced Schwann cells. Overexpressing miR-363-5p could improve pain threshold and alleviate inflammation in CCI rats. It also a ttenuated LPS-induced inflammation and reduced proliferation in Schwann cells. The overexpression of SERPING1 could reverse the protective effect of miR-363-5p on CCI rats and LPS-induced Schwann cell injury.Conclusion: miR-363-5p protected from neuropathic pain via alleviating Schwann cell injury by negatively modulating SERPING1.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"35-43"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and safety of galcanezumab for cluster headache preventive treatment: a systematic review and meta-analysis. galcanezumab用于丛集性头痛预防治疗的有效性和安全性：一项系统回顾和荟萃分析。

IF 1.7 4区医学

Neurological Research Pub Date : 2025-01-01 Epub Date: 2024-12-17 DOI: 10.1080/01616412.2024.2440022

Marina Barbosa da Silva, Domênica Baroni Coelho de Oliveira Ferreira, Cristian D Mendieta, Hamilcar Pereira da Silva, Lívia Aguiar Nogueira-Rosa, Nathalia Moraes-Figueiredo

{"title":"Efficacy and safety of galcanezumab for cluster headache preventive treatment: a systematic review and meta-analysis.","authors":"Marina Barbosa da Silva, Domênica Baroni Coelho de Oliveira Ferreira, Cristian D Mendieta, Hamilcar Pereira da Silva, Lívia Aguiar Nogueira-Rosa, Nathalia Moraes-Figueiredo","doi":"10.1080/01616412.2024.2440022","DOIUrl":"10.1080/01616412.2024.2440022","url":null,"abstract":"Background: Galcanezumab is a monoclonal antibody targeting the CGRP pathway and represents the latest disease-specific and mechanism-based therapeutic option for cluster headache (CH).Objective: We performed a systematic review and meta-analysis to evaluate the efficacy and safety of galcanezumab for CH.Methods: We searched PubMed, Embase, and Cochrane Library for studies implementing galcanezumab for episodic and chronic CH. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) and Reporting Guidelines for Meta-analyses of Observational Studies (MOOSE) guidelines. The primary outcome was efficacy, defined by a reduction from the baseline of at least 50% in the weekly frequency of CH attacks and the Patient Global Impression of Improvement scale (PGI-I). Secondary outcomes included treatment-emergent adverse events (TEAEs) and serious adverse events (SAEs).Results: A total of 504 patients were included from 6 studies, of which 2 were RCTs. The follow-up period ranged from 3 weeks to 15 months. The mean age was 44.4 ± 10.2 years, with 24.4% female patients. Overall efficacy was 76.0% (95% CI 0.67-0.85), TEAEs were observed in 48.0% of patients (95% CI 0.25-0.72), and the most common were nasopharyngitis, local injection pain, and local injection swelling. TEAEs were, however, considerably higher within the 300 mg dose group compared with the 240 mg dose group, 80.0% (95% CI 0.65-0.87) versus 28.0% (95% CI 0.12-0.47), respectively.Conclusion: This meta-analysis suggests that galcanezumab is effective in reducing the number of CH attacks and can be considered a safe medication.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"63-76"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142838379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Auditory evoked brainstem responses and medial olivocochlear efferent system in migraine patients with phonophobia. 偏头痛患者的听觉诱发脑干反应和橄榄耳内侧传出系统

IF 1.7 4区医学

Neurological Research Pub Date : 2025-01-01 Epub Date: 2024-11-20 DOI: 10.1080/01616412.2024.2430994

Emre Soylemez, Aydın Sinan Apaydin, Tugce Gurel Soylemez, Borna Farhoomand, Dursun Ceylan

{"title":"Auditory evoked brainstem responses and medial olivocochlear efferent system in migraine patients with phonophobia.","authors":"Emre Soylemez, Aydın Sinan Apaydin, Tugce Gurel Soylemez, Borna Farhoomand, Dursun Ceylan","doi":"10.1080/01616412.2024.2430994","DOIUrl":"10.1080/01616412.2024.2430994","url":null,"abstract":"Objective: This study aims to investigate Auditory Evoked Brainstem Responses (ABR) and Distortion Product Otoacoustic Emission (DPOAE) suppression in migraine patients with and without phonophobia.Methods: Thirty-two migraine patients with normal hearing and 30 healthy individuals were included in the study. Migraine characteristics and phonophobia status of migraine patients were noted. The patients were divided into two groups according to their phonophobia status. All participants underwent ABR, DPOAE and DPOAE suppression.Results: Migraine patients had less DPOAE suppression (1481 and 2222 hz) and shorter ABR wave latencies compared to the control group (p < 0.05). Twelve (37.5%) of the migraine patients did not have phonophobia, and 20 (62.5%) had phonophobia. Phonophobia was not found to affect DPOAE suppression (p > 0.05). However, ABR wave I and V latencies in migraine patients with phonophobia were shorter than in healthy individuals (p < 0.05).Conclusion: There are changes in the auditory evoked brainstem responses and medial olivocochlear efferent system of migraine patients. While phonophobia in migraine patients does not affect the medial olivocochlear efferent system, it may affect auditory evoked brainstem responses.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"7-14"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of D-dimer/fibrinogen ratio and combination of D-dimer and fibrinogen with prognosis of stroke and stroke subtypes. d -二聚体/纤维蛋白原比值及d -二聚体与纤维蛋白原联合与脑卒中及脑卒中亚型预后的关系

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-31 DOI: 10.1080/01616412.2024.2448630

Yun Zhai, Mengmeng Huo, Yue Liu, Hongwei Sun, Yanyan Sun, Fang Li, Hongwei Sun, Ying Tang

{"title":"Association of D-dimer/fibrinogen ratio and combination of D-dimer and fibrinogen with prognosis of stroke and stroke subtypes.","authors":"Yun Zhai, Mengmeng Huo, Yue Liu, Hongwei Sun, Yanyan Sun, Fang Li, Hongwei Sun, Ying Tang","doi":"10.1080/01616412.2024.2448630","DOIUrl":"https://doi.org/10.1080/01616412.2024.2448630","url":null,"abstract":"Objective: Here, we aim to investigate whether D-dimer (DD)/fibrinogen (FIB) ratio or combination of DD and FIB contribute to the prognosis of stroke and stroke subtypes.Methods: 1413 patients with acute ischemic stroke (AIS) were recruited. We measured DD and FIB levels on admission and followed up with patients at discharge and 90-day following discharge. We analyzed the association between DD/FIB ratio and poor function outcome of AIS and different AIS subtypes. Similarly, logistic regression model was used to estimate the combined effect of DD level and FIB level on the poor outcomes of stroke and stroke subtypes.Results: The patients with DD+FIB+ or high DD/FIB ratio tended to have the high risk of severe neurological deficits at both discharge and 90-day following discharge. In the subgroup analysis, high DD/FIB ratio was significantly associated with the poor function outcome in cardioembolism (CE) and large-artery atherosclerosis (LAA) subtypes. DD+FIB+ was strongly associated with the poor function outcome in CE subtype at discharge and 90-day.Conclusion: DD/FIB ratio and combination of DD and FIB may have more significant prognostic value of stroke and stroke subtypes than either index of DD or FIB alone in AIS patients.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobile stroke units based on rural emergency medical stations for pre-hospital intravenous thrombolysis of stroke in remote areas. 基于农村急救站的流动卒中单元，用于偏远地区卒中的院前静脉溶栓治疗。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-10-30 DOI: 10.1080/01616412.2024.2423588

Bin Liu, Pengcheng Zhang, Hongyun Wang, Qianqian Miao, Shuaishuai Wang, Wenting Zhang

{"title":"Mobile stroke units based on rural emergency medical stations for pre-hospital intravenous thrombolysis of stroke in remote areas.","authors":"Bin Liu, Pengcheng Zhang, Hongyun Wang, Qianqian Miao, Shuaishuai Wang, Wenting Zhang","doi":"10.1080/01616412.2024.2423588","DOIUrl":"10.1080/01616412.2024.2423588","url":null,"abstract":"Objectives: To explore a novel model of mobile stroke units (MSUs) integrated with rural emergency medical stations for pre-hospital care of stroke patients in remote areas.Methods: We used MSUs + Ambulance mode, where both the MSUs and conventional ambulances are sent to the patient's location. The conventional ambulance coordinates with the MSUs to choose the fastest route to meet and transfer the patient at the point along the way. We collected data from 149 patients from March 2022 to April 2023, including National Institutes of Health Stroke Scale (NIHSS) scores (on admission, 24 hours, day 7), 90-day modified Rankin Scale (mRS) scores, and other clinical variables. We performed propensity score matching (PSM) to balance the potential confounding variables between groups.Results: We found that the MSUs + Ambulance mode (OR = 12.507, 95% confidence interval [CI] [3.633, 43.061], p < 0.001) and admission NIHSS score (OR = 0.583, 95% CI [0.493, 0.690], p < 0.001) were independent prognostic risk factors for stroke patients. The MSUs + Ambulance mode reduced NIHSS scores 7 days prior to admission (OR = 0.679, 95% CI [0.563, 0.819], p < 0.001). After PSM, patients who received MSUs + Ambulance mode had a better prognosis (χ2 = 9.573, p = 0.004), as well as a lower mRS score at 90 days (Z = -3.371, p = 0.001).Conclusions: MSUs integrated with rural emergency medical stations show the feasibility and potential benefits of pre-hospital intravenous thrombolysis for stroke patients in geographically distant regions.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1160-1166"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of unsuccessful reactive responses to lateral loss of balance in people with stroke. 中风患者对横向失去平衡的不成功反应的特征。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-08-21 DOI: 10.1080/01616412.2024.2394327

Shirley Handelzalts, Nachum Soroker, Itshak Melzer

{"title":"Characteristics of unsuccessful reactive responses to lateral loss of balance in people with stroke.","authors":"Shirley Handelzalts, Nachum Soroker, Itshak Melzer","doi":"10.1080/01616412.2024.2394327","DOIUrl":"10.1080/01616412.2024.2394327","url":null,"abstract":"Purpose: The effectiveness of reactive responses to a sudden loss of balance is a critical factor that determines whether a fall will occur. We examined the strategies and kinematics associated with successful and unsuccessful balance recovery following lateral loss of balance in people with stroke (PwS).Methods: Eleven PwS were included in the analysis. They were exposed to unannounced right and left horizontal surface translations and demonstrated both successful and unsuccessful balance responses at the same perturbation magnitude. Reactive step strategies and kinematics were investigated comparatively in successful and unsuccessful recovery tests.Results: The crossover strategy was used in most of the unsuccessful tests (7/11) while the unloaded-leg side-step in the successful tests (6/11). There were no significant differences in the reactive step initiation time in unsuccessful vs. successful tests. However, the step execution time, step length and center of mass displacement were significantly higher during the first recovery step in unsuccessful tests.Conclusions: PwS have difficulties in controlling and decelerating the moving center of mass following a lateral loss of balance. The increased step time and step length of the first reactive step in unsuccessful vs. successful tests suggest the crossover step strategy may be ineffective for PwS.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1095-1104"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between skeletal muscle mass change and swallowing function improvement among stroke patients with dysphagia during rehabilitation. 有吞咽困难的中风患者在康复期间骨骼肌质量变化与吞咽功能改善之间的关系。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-11-20 DOI: 10.1080/01616412.2024.2423587

Kenta Kudaka, Keisuke Sato, Yuhki Nakayama, Masaki Koike, Takahiro Ogawa

引用次数: 0

Dexmedetomidine inhibits the migration, invasion, and glycolysis of glioblastoma cells by lactylation of c-myc. 右美托咪定通过乳化 c-myc 抑制胶质母细胞瘤细胞的迁移、侵袭和糖酵解。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-08-28 DOI: 10.1080/01616412.2024.2395069

Jianglian Zhu, Yundong Zhang

{"title":"Dexmedetomidine inhibits the migration, invasion, and glycolysis of glioblastoma cells by lactylation of c-myc.","authors":"Jianglian Zhu, Yundong Zhang","doi":"10.1080/01616412.2024.2395069","DOIUrl":"10.1080/01616412.2024.2395069","url":null,"abstract":"Background: Glioblastoma (GBM) is a brain tumor with poor prognosis. Dexmedetomidine (Dex) regulates the biological behaviors of tumor cells to accelerate or decelerate cancer progression.Objective: We investigated the effects of Dex on the migration, invasion, and glycolysis in GBM.Methods: The concentration of Dex was determined using the cell counting kit-8 assay. The impacts of Dex on biological behaviors of GBM cells were assessed using Transwell assay, XF96 extracellular flux analysis, and western blot. The expression of c-Myc was examined using reverse transcription-quantitative polymerase chain reaction. The lactylation or stability of c-Myc was measured by western blot after immunoprecipitation or cycloheximide treatment.Results: We found that Dex (200 nM) inhibited GBM cell viability, migration, invasion, and glycolysis. C-Myc was highly expressed in GBM cells and was decreased by Dex treatment. Moreover, Dex suppressed lactylated c-Myc levels via suppressing glycolysis, thereby reducing the protein stability of c-Myc. Sodium lactate treatment abrogated the effects of Dex on the biological behaviors of GBM cells.Conclusion: Dex suppressed the migration, invasion, and glycolysis of GBM cells via inhibiting lactylation of c-Myc and suppressing the c-Myc stability, suggesting that Dex may be a novel therapeutic drug for GBM treatment.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1105-1112"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of restless genital syndrome and its impact on quality of life in women with restless legs syndrome. 患有不宁腿综合征的女性中生殖器不宁综合征的患病率及其对生活质量的影响。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-11-07 DOI: 10.1080/01616412.2024.2424574

Filiz Manga Günaydin, Abdulkadir Tunç

{"title":"Prevalence of restless genital syndrome and its impact on quality of life in women with restless legs syndrome.","authors":"Filiz Manga Günaydin, Abdulkadir Tunç","doi":"10.1080/01616412.2024.2424574","DOIUrl":"10.1080/01616412.2024.2424574","url":null,"abstract":"Background: Restless genital syndrome (RGS), also known as persistent genital arousal disorder, is a distressing condition characterized by unwanted genital arousal in the absence of sexual desire. This study explores the prevalence of RGS in women with restless legs syndrome (RLS), investigates the associated psychological impacts, and assesses the overall effect on quality of life.Methods: This retrospective study included 69 female patients who were diagnosed with RLS at two university medical centers. Data were collected via the RGS diagnostic form, Beck Depression Inventory, Beck Anxiety Inventory, and WHOQOL-BREF quality of life scale. Statistical analyses were used to assess the correlation between RGS and psychological health measures.Results: Approximately 44.9% of the participants with RLS also reported symptoms of RGS. Significant findings included increased nighttime and rest-related exacerbation of RGS symptoms. Compared with those without RGS, participants with RGS presented significantly higher anxiety and depression scores. Moreover, RGS significantly impacted physical health and social relationships, as indicated by lower WHOQOL-BREF scores.Conclusion: This study highlights a significant overlap between RGS and RLS, with substantial impacts on psychological well-being and quality of life. These findings underscore the importance of considering RGS in the clinical management of RLS, suggesting a need for integrated treatment strategies to address both the neurological and the psychological aspects of these conditions.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1174-1180"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dimethyl itaconate modulates neuroprotective effect on primary rat astrocytes under inflammatory condition by regulating the expression of neurotrophic factors and TrkA/B-P75 receptors. 衣康酸二甲酯通过调节神经营养因子和TrkA/B-P75受体的表达，调节炎症条件下原代大鼠星形胶质细胞的神经保护作用

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-11-03 DOI: 10.1080/01616412.2024.2423583

Nooshin Azari, Malahat Rezaee, Dian Dayer, Mohammad Reza Tabandeh

{"title":"Dimethyl itaconate modulates neuroprotective effect on primary rat astrocytes under inflammatory condition by regulating the expression of neurotrophic factors and TrkA/B-P75 receptors.","authors":"Nooshin Azari, Malahat Rezaee, Dian Dayer, Mohammad Reza Tabandeh","doi":"10.1080/01616412.2024.2423583","DOIUrl":"10.1080/01616412.2024.2423583","url":null,"abstract":"Introduction: Astrocytes, specialized glial cells, are essential for maintaining the central nervous system homeostasis. Inflammatory conditions can disrupt neurotrophic factors and receptor expression in astrocytes, leading to potential central nervous system damage. Itaconate, recently identified for its anti-inflammatory properties, was investigated in this study for its effects on neurotrophic factors in LPS-stimulated primary rat astrocytes.Methods: Primary rat astrocyte cells were isolated from one-day-old Wistar rats and exposed to 1 µg/ml lipopolysaccharide (LPS) for 6 h to stimulate inflammation. The effect of DMI (62.5, 125, and 250 µM for 18 h) on the cell viability of astrocyte cells exposed to LPS was evaluated by the MTT assay. The effects of DMI on the mRNA and protein levels of NGF, BDNF, and GDNF were evaluated using ELISA and qRT-PCR assays. Protein and mRNA levels of neurotrophic factor receptors (TrkA, TrkB, and P75) were evaluated using qRT-PCR and Western blot analyses.Results: The results showed that DMI suppressed astrocytes cell death induced by LPS in a dose-dependent manner. DMI dose-dependently restored the reduced mRNA and protein levels of NGF, BDNF, GDNF, and TrkA and TrkB receptors in LPS-treated astrocytes, but it significantly decreased the p75 expression in the same condition.Conclusion: In conclusion, DMI may be able to support astrocyte survival and functions based on the restoration of neurotrophic factors and their receptors expression in LPS-stimulated astrocyte cells. This suggests that DMI could be a promising therapeutic option for neurodegenerative diseases characterized by inflammation-induced astrocyte dysfunction.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1137-1148"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0