Abnormal expression of miR-136-5p in glioma cancer patients and its correlation with CT signs.

Background: MicroRNAs (miRNAs) have attracted considerable attention as promising new tumor biomarkers.

Aims: This study primarily integrates computed tomography (CT) findings to investigate the expression and potential implications of miR-136-5p in glioma.

Methods: Human glioma samples were obtained from 122 glioma tissues and 80 adjacent tissues resected by surgery from confirmed patients. Reverse transcription quantitative PCR (RT-qPCR) was used to detect the expression of the genes. Receiver Operating Characteristic (ROC) curve was used to evaluate the diagnostic value of miR-136-5p combined with Computed Tomography (CT) in glioma. Correlation analysis was conducted employing Pearson's method. The target genes of miR-136-5p were identified using the TargetScan online database and validated through dual luciferase reporter assays.

Results: In glioma tissues, the expression of miR-136-5p was markedly downregulated. Its expression correlates with CT signs and clinicopathological features of glioma patients. Both CT detection and miR-136-5p expression showed significant potential in diagnosing glioma, and combining the two improved the diagnostic value of glioma. Furthermore, the overexpression of miR-136-5p has been shown to negatively regulate the expression of the BCL9L gene in U87 and U251 glioma cell lines, thereby inhibiting tumor cell proliferation and promoting apoptotic processes.

Conclusions: miR-136-5p is downregulated in glioma tissues and closely correlates with tumor CT signs. It may serve as a promising biomarker to assist in glioma diagnosis using CT scans.