Neurological Research最新文献_第3页

Exploring the correlation between dietary zinc intake and stroke risk in adults based on NHANES database. 基于 NHANES 数据库，探讨成人膳食锌摄入量与中风风险之间的相关性。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-11-07 DOI: 10.1080/01616412.2024.2403858

Lingyun Huang, Yongjun Chen, Juanchan Sun, Li Xu

{"title":"Exploring the correlation between dietary zinc intake and stroke risk in adults based on NHANES database.","authors":"Lingyun Huang, Yongjun Chen, Juanchan Sun, Li Xu","doi":"10.1080/01616412.2024.2403858","DOIUrl":"10.1080/01616412.2024.2403858","url":null,"abstract":"Objective: To explore the relationship between dietary zinc intake and stroke.Methods: Subjects from the National Health and Nutrition Examination Survey (NHANES) database (2015 to 2020) were included. Zinc intake was determined using two 24-h dietary recall interviews, and stroke was determined using the Medical Condition Questionnaire (MCQ). Logistic analysis was used to analyze the association between zinc intake and stroke risk. 1:1 nearest neighbor propensity score matching (PSM) was used to reduce selection bias.Results: 4705 subjects were included in the study. Multivariate logistic regression analysis before and after matching showed that increased zinc intake was associated with a reduced risk of stroke. And as zinc intake increases, the risk of stroke shows a gradually decreasing trend. Compared with the Q1 group, the risk of stroke in the Q2, Q3, and Q4 groups was reduced by approximately 0.27 times, 0.29 times, and 0.31 times respectively. And there is no interaction between dietary zinc intake and gender in stroke patients.Conclusion: Dietary zinc intake may be a protective factor against stroke, and increasing its intake may prevent or reduce the symptoms of stroke and related diseases.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1113-1121"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-429 overexpression overcomes imatinib resistance of glioma cells by negatively regulating lysophosphatidic acid receptor 1. 通过负调控溶血磷脂酸受体1，过表达MicroRNA-429可克服胶质瘤细胞对伊马替尼的耐药性。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-11-12 DOI: 10.1080/01616412.2024.2423586

Jieyao Xia, Zhengyang Peng, Meina Zhang, Qiongqiong Liao, Chubao Liu, Xiong Deng

{"title":"MicroRNA-429 overexpression overcomes imatinib resistance of glioma cells by negatively regulating lysophosphatidic acid receptor 1.","authors":"Jieyao Xia, Zhengyang Peng, Meina Zhang, Qiongqiong Liao, Chubao Liu, Xiong Deng","doi":"10.1080/01616412.2024.2423586","DOIUrl":"10.1080/01616412.2024.2423586","url":null,"abstract":"Background: Glioma is one of the most aggressive and lethal malignancies in central nervous system. It has been reported that miR-429 is declined in glioma and functions as a tumor suppressor. Nonetheless, the potential role of miR-429 in drug resistance of glioma is still ambiguous.Methods: Stable imatinib-resistant lines U251-AR and T98G-AR were established using glioma cell lines U251 and T98G. Cell apoptosis and cycle were analyzed by flow cytometry, and CCK-8 assay was utilized to measure cell viability. Protein and RNA levels were tested with western blot and RT-qPCR. The predicted binding site was confirmed by dual luciferase reporter assay.Results: Imatinib-resistant U251-AR and T98G-AR cells presented lower level of miR-429 and higher level of LPAR1. MiR-429 overexpression obviously promoted imatinib sensitivity in glioma cells, indicated by the reduced IC50 value, facilitated cell apoptosis and cell cycle arrest at G0/G1 phase, and downregulated multidrug resistance-related proteins. LPAR1 was verified as a direct target of miR-429 and its expression was negatively regulated by miR-429. Additionally, overexpression of LPAR1 restrained the biological function of miR-429 on imatinib chemoresistance.Conclusion: MiR-429 partly sensitized glioma cells to imatinib via downregulation LPAR1, which might provide an approach to overcome imatinib chemoresistance during glioma treatment.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1149-1159"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of deep brain stimulation on sexual dysfunction among patients who had Parkinson's disease: a systematic review and meta-analysis. 脑深部刺激对帕金森病患者性功能障碍的影响：系统回顾和荟萃分析。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-12-01 Epub Date: 2024-10-01 DOI: 10.1080/01616412.2024.2407645

Amir Reza Bahadori, Rasa Zafari, Mohammad Amin Fathollahi, Afshan Davari, Mehrdad Sheikhvatan, Sara Ranji, Abbas Tafakhori

{"title":"Effect of deep brain stimulation on sexual dysfunction among patients who had Parkinson's disease: a systematic review and meta-analysis.","authors":"Amir Reza Bahadori, Rasa Zafari, Mohammad Amin Fathollahi, Afshan Davari, Mehrdad Sheikhvatan, Sara Ranji, Abbas Tafakhori","doi":"10.1080/01616412.2024.2407645","DOIUrl":"10.1080/01616412.2024.2407645","url":null,"abstract":"Background: Patients who have Parkinson's disease (PD) present several non-motor issues, such as sexual dysfunction. Deep brain stimulation (DBS) is a great treatment for PD and could affect both motor and non-motor symptoms of patients.Aim: The main goal of the current study is to evaluate the impact of DBS on the sexual dysfunction among patients with PD.Methods: Five databases (PubMed, Scopus, Embase, Web of Science, and Cochrane Library) were searched for records. Studies that measured the effect of DBS sexual function were included. The risk of bias assessment tool of non-randomized studies of interventions (ROBINS-I) was used to assess the quality of the included studies. The before and after data extraction and statistical analysis were performed using the Comprehensive Meta-analysis software (CMA) version 3.0.Result: Ten studies were included in the systematic review; six of them were eligible to perform a meta-analysis with a total sample size of 532 participants and a mean age of 62.21 ± 1.59 years. All participants performed STN-DBS. The sexual function of participants after STN-DBS implantation significantly increased (SMD = -0.124, 95% CI: -0.209 to -0.038, P-value = 0.005). It also did not have any publication bias. Additionally, their quality of life mounts significantly (SMD = -0.712, 95% CI: -1.002 to -0.422, p-value <0.001).Conclusion: Our systematic review highlights the potential effect of STN-DBS on reducing the sexual dysfunction of patients with PD and boosting their quality of life.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1181-1190"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viscolin-mediated antiapoptotic and neuroprotective effects in cortical neurons exposed to oxygen-glucose deprivation and rats subjected to transient focal cerebral ischemia. 粘胶素介导的抗缺氧-缺糖大脑皮层神经元凋亡和神经保护作用以及对短暂局灶性脑缺血大鼠的影响

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-20 DOI: 10.1080/01616412.2024.2381381

Hao-Hsiang Hsu, Ai-Hua Lee, Shih-Huang Tai, Liang-Yi Chen, Sheng-Yang Huang, Yi-Yun Chen, Yu-Chang Hung, Tian-Shung Wu, E-Jian Lee

{"title":"Viscolin-mediated antiapoptotic and neuroprotective effects in cortical neurons exposed to oxygen-glucose deprivation and rats subjected to transient focal cerebral ischemia.","authors":"Hao-Hsiang Hsu, Ai-Hua Lee, Shih-Huang Tai, Liang-Yi Chen, Sheng-Yang Huang, Yi-Yun Chen, Yu-Chang Hung, Tian-Shung Wu, E-Jian Lee","doi":"10.1080/01616412.2024.2381381","DOIUrl":"10.1080/01616412.2024.2381381","url":null,"abstract":"Objective: Previously, we have successfully purified and synthesized viscolin, an agent derived from Viscum coloratum extract, which has shown significant potential in the treatment of stroke. Our study aimed to evaluate the neuroprotective effects of viscolin.Methods: We first assessed the cytotoxicity of viscolin on primary neuronal cultures and determined its antioxidant and radical scavenging properties. Subsequently, we identified the optimal dose-response of viscolin in protecting against glutamate-induced neurotoxicity.Results: Our results demonstrated that viscolin at a concentration of 10 μM effectively reduced neuronal cell death up to 6 hours after glutamate-induced neurotoxicity. Additionally, we investigated the therapeutic window of opportunity and the potential of viscolin in preventing necrotic and apoptotic damage in cultured neurons exposed to oxygen glucose deprivation-induced neurotoxicity. Our findings showed that viscolin treatment significantly reduced DNA breakage, prevented the release of cytochrome c from mitochondria to cytosol, increased the expression of anti-apoptotic protein Bcl-2, decreased the expression of pro-apoptotic protein Bax, and reduced the number of TUNEL-positive cells. Additionally, our in vivo investigation demonstrated a reduction in brain infarction following middle cerebral artery occlusion.Conclusion: Viscolin has potential utility as a therapeutic agent in the treatment of stroke.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1063-1073"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of world stroke campaign on stroke literacy and knowledge in Taiwan. 世界卒中运动对台湾卒中素养和知识的影响。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-08-01 DOI: 10.1080/01616412.2024.2381159

Bing-Yan Jiang, Lung Chan, Hung-Chou Chen, Ka-Wai Tam, Tsan-Hon Liou, Reuben Escorpizo, Li-Fong Lin

{"title":"Effects of world stroke campaign on stroke literacy and knowledge in Taiwan.","authors":"Bing-Yan Jiang, Lung Chan, Hung-Chou Chen, Ka-Wai Tam, Tsan-Hon Liou, Reuben Escorpizo, Li-Fong Lin","doi":"10.1080/01616412.2024.2381159","DOIUrl":"10.1080/01616412.2024.2381159","url":null,"abstract":"Objectives: Stroke is a leading cause of death in Taiwan. Poor public knowledge of stroke may lead to delays in prehospital arrival, resulting in unfavorable prognoses. Studies have investigated public knowledge of stroke and highlighted the importance of stroke education, however, few such studies have been conducted in Taiwan. This study assessed the changes in public knowledge of stroke between 2012 and 2020 by conducting a survey during two World Stroke Day events. Furthermore, this study identified areas where educational efforts may have been insufficient.Materials & methods: Questionnaires were distributed to the participants of 2012 and 2020 World Stroke Day events in Taiwan. In total, 328 and 336 questionnaires were completed, respectively. Stroke literacy and knowledge were analyzed between 2012 and 2020. Data were analyzed using the chi-square test or independent t-test. p < 0.05 indicates statistical significance.Results: Hypertension was the most recognized risk factor for stroke in both years (p < 0.001), and recognition of most of the given risk factors significantly increased. In addition, recognition of more than half of the stroke warning signs significantly increased, awareness of the correct acute stroke response also increased (p < 0.001), and overall stroke literacy in Taiwan increased (p = 0.001).Conclusion: Stroke literacy and knowledge in Taiwan have improved significantly between 2012 and 2020, but many people still lack adequate stroke knowledge and awareness. Government health department must take this sort of intervention continually (campaigns) and novel approaches (e.g. board game…) to improve stroke literacy and knowledge in public health.Registration id: N202109072, approved by the Joint Institutional Review Board of Taipei Medical University on 2021/11/02.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1026-1036"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-intensity interval training stimulates remyelination via the Wnt/β-catenin pathway in cuprizone-induced demyelination mouse model. 在铜绿素诱导的脱髓鞘小鼠模型中，高强度间歇训练可通过 Wnt/β-catenin 通路刺激髓鞘再形成。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-09 DOI: 10.1080/01616412.2024.2376310

Fei Chen, Bing Cheng, Xinqi Xu, Weixing Yan, Qiqi Meng, Jinfeng Liu, Ruiqin Yao, Fuxing Dong, Yaping Liu

{"title":"High-intensity interval training stimulates remyelination via the Wnt/β-catenin pathway in cuprizone-induced demyelination mouse model.","authors":"Fei Chen, Bing Cheng, Xinqi Xu, Weixing Yan, Qiqi Meng, Jinfeng Liu, Ruiqin Yao, Fuxing Dong, Yaping Liu","doi":"10.1080/01616412.2024.2376310","DOIUrl":"10.1080/01616412.2024.2376310","url":null,"abstract":"Objectives: This study aims to investigate the role of high-intensity interval training (HIIT) in promoting myelin sheath recovery during the remyelination phase in cuprizone (CPZ)-induced demyelination mice and elucidate the mechanisms involving the Wnt/β-catenin pathway.Methods: After 5 weeks of a 0.2% CPZ diet to induce demyelination, a 4-week recovery phase with a normal diet was followed by HIIT intervention. Mice body weight was monitored. Morris water maze (MWM) gauged spatial cognition and memory, while the open field test (OFT) assessed anxiety levels. Luxol fast blue (LFB) staining measured demyelination, and immunofluorescence examined myelin basic protein (MBP) and platelet-derived growth factor receptor-alpha (PDGFR-α). Western blotting analyzed protein expression, including MBP, PDGFR-α, glycogen synthase kinase-3β (GSK3β), β-catenin, and p-β-catenin. Real-time PCR detected mRNA expression levels of CGT and CST.Results: HIIT promoted remyelination in demyelinating mice, enhancing spatial cognition, memory, and reducing anxiety. LFB staining indicated decreased demyelination in HIIT-treated mice. Immunofluorescence demonstrated increased MBP fluorescence intensity and PDGFR-α+ cell numbers with HIIT. Western blotting revealed HIIT reduced β-catenin levels while increasing p-β-catenin and GSK3β levels. Real-time PCR demonstrated that HIIT promoted the generation of new myelin sheaths. Additionally, the Wnt/β-catenin pathway agonist, SKL2001, decreased MBP expression but increased PDGFR-α expression.Discussion: HIIT promotes remyelination by inhibiting the Wnt/β-catenin pathway and is a promising rehabilitation training for demyelinating diseases. It provides a new theoretical basis for clinical rehabilitation and care programs.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"996-1007"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Awareness of risk factors for cerebrovascular diseases among acute ischemic stroke patients in Shenzhen, China. 中国深圳急性缺血性脑卒中患者对脑血管疾病危险因素的认识。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-26 DOI: 10.1080/01616412.2024.2381161

Shiyu Hu, Zhihao Lei, Yang Wang, Lijie Ren

{"title":"Awareness of risk factors for cerebrovascular diseases among acute ischemic stroke patients in Shenzhen, China.","authors":"Shiyu Hu, Zhihao Lei, Yang Wang, Lijie Ren","doi":"10.1080/01616412.2024.2381161","DOIUrl":"10.1080/01616412.2024.2381161","url":null,"abstract":"Background and purpose: Unawareness of the risk factors is one of the most important issues that need to be settled for stroke prevention. We aimed to evaluate the awareness of risk factors for cerebrovascular diseases (CVDs) among acute ischemic stroke patients and to investigate the characteristics of patients who were unaware of their risk factors in Shenzhen, China.Methods: Registered data on awareness of CVD risk factors of patients with confirmed acute ischemic stroke (AIS) from June 2020 to December 2022 were analyzed in May 2023. The data were extracted from the database of Shenzhen Quality Control Center for Management of Cerebrovascular Diseases.Results: Totally, there were 5147 AIS patients with complete data eligible for this study. AIS patients' awareness regarding existing hypertension, diabetes, dyslipidemia, and atrial fibrillation (AF) was 76.1%, 76.2%, 24.2%, and 53.4%, respectively. Patients who were lack of awareness of the CVD risk factors were more likely to be males, individuals with younger ages, and those without medical insurance or a CVD history.Conclusions: The overall awareness of the CVD risk factors was suboptimal among AIS patients in Shenzhen, especially for the existing dyslipidemia. The health education of AIS should be further improved in males as well as individuals without medical insurance or any CVD histories. Age was an independent factor associated with the lack of awareness of the CVD risk factors. The stroke screening program should be extended to younger people.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1046-1053"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exoskeleton-assisted upper limb rehabilitation after stroke: a randomized controlled trial. 外骨骼辅助中风后上肢康复：随机对照试验。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-26 DOI: 10.1080/01616412.2024.2381385

İrem Akgün, İlkşan Demirbüken, Eren Timurtaş, Mehmet Kutay Pehlivan, Ali Utku Pehlivan, Mine Gülden Polat, Gerard E Francisco, Nuray Yozbatiran

{"title":"Exoskeleton-assisted upper limb rehabilitation after stroke: a randomized controlled trial.","authors":"İrem Akgün, İlkşan Demirbüken, Eren Timurtaş, Mehmet Kutay Pehlivan, Ali Utku Pehlivan, Mine Gülden Polat, Gerard E Francisco, Nuray Yozbatiran","doi":"10.1080/01616412.2024.2381385","DOIUrl":"10.1080/01616412.2024.2381385","url":null,"abstract":"Objectives: The upper-limb exoskeleton training program which is repetetive and task-specific therapy can improve motor functions in patients with stroke. To compare the effect of an upper-limb exoskeleton training program with Bobath concept on upper limb motor functions in individuals with chronic stroke.Methods: Participants were randomly assigned to exoskeleton group (EG, n = 12) or to Bobath group (BG, n = 12). Interventions were matched in terms of session duration and total number of sessions and performed 2 times per week for 6-weeks. Primary outcome was Fugl-Meyer-Upper Extremity (FMA-UE). Secondary outcomes were Modified Ashworth Scale (elbow and wrist flexor muscles), Motor Activity Log-30 which is consist of two parts as an amount of use (AOU) and quality of movement (QOM), and The Nottingham Extended Activities of Daily Living (NEADL) index.Results: After 12-sessions of training, the mean (SD) FMA-UE score increased by 5.7 (2.9) in the EG, and 1.9 (1.5) points in the BG (p < .05). In total, 40% of participants (5/12) demonstrated a clinically meaningful improvement (≥5.25 points) in the FM-UE, while none of the participants reached MCID score in the bobath group. Changes in the AOU, QOM, and NEADL were significantly larger in the EG compared to BG (p < .05). 7/12 (58.33%) of participants for AOU and 5/12 (42%) of participants for QOM in the EG showed that clinically meaningful change. 5/12 of participants (42%) in the EG demonstrated ≥4.9-point increase in NEADL score.Discussion: High-intensity repetitive arm and hand exercises with an exoskeleton device was safe and feasible. Exoskeleton-assisted training demonstrated significant benefits in improving upper limb functions and quality of life in individuals after stroke.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1074-1082"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141760066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circ_0076490 silencing inhibits MAPK1 expression to decrease the proliferation and increase apoptosis of Jurkat cells by regulating miR-144-3p in myasthenia gravis. 在肌无力患者中，Circ_0076490沉默可通过调节miR-144-3p抑制MAPK1的表达，从而减少Jurkat细胞的增殖并增加其凋亡。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-08-29 DOI: 10.1080/01616412.2024.2394324

Qin Ye, Chengyao Gu, Wang Yan

{"title":"Circ_0076490 silencing inhibits MAPK1 expression to decrease the proliferation and increase apoptosis of Jurkat cells by regulating miR-144-3p in myasthenia gravis.","authors":"Qin Ye, Chengyao Gu, Wang Yan","doi":"10.1080/01616412.2024.2394324","DOIUrl":"10.1080/01616412.2024.2394324","url":null,"abstract":"Background: Myasthenia gravis (MG) is a both neuromuscular junction and antibody-mediated autoimmune disease, and its pathogenesis involves the regulation of circular RNAs (circRNAs). However, the role of circ_0076490 in MG and the underlying mechanism remain unknown.Methods: RNA levels of circ_0076490, microRNA-144-3p (miR-144-3p), and mitogen-activated protein kinase 1 (MAPK1) were detected by quantitative real-time polymerase chain reaction. Cell viability and proliferation were investigated by cell counting kit-8 and 5-Ethynyl-29-deoxyuridine assays, respectively. Cell cycle and apoptosis were assessed by flow cytometry analysis. The putative binding relationship of miR-144-3p and circ_0076490 or MAPK1 was predicted by circular RNA interactome and TargetScan online databases, respectively, and identified through dual-luciferase reporter assay and RNA pull-down assay.Results: We observed dramatic increases of circ_0076490 and MAPK1 expression and a decrease of miR-144-3p expression in the peripheral blood of MG patients in comparison with healthy controls. Reduced expression of circ_0076490 induced an inhibitory effect on the proliferation of Jurkat cells and a promoting effect on cell apoptosis. Additionally, miR-144-3p was identified as a target miRNA of circ_0076490, and its depletion attenuated circ_0076490 knockdown-mediated effects on the proliferation and apoptosis of Jurkat cells. MAPK1 was a target gene of miR-144-3p and its overexpression rescued decreased cell proliferation and increased cell apoptosis induced by miR-144-3p introduction. Furthermore, circ_0076490 regulated MAPK1 expression by interacting with miR-144-3p.Conclusion: Circ_0076490 knockdown inhibited proliferation and induced apoptosis of Jurkat cells through the regulation of the miR-144-3p/MAPK1 axis, providing potential targets for developing improved therapy of MG.","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"1083-1093"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of YTHDF1 (not YTHDF3) reduced brain and gut damage after traumatic brain injury. YTHDF1（而非YTHDF3）的缺失可减少脑外伤后大脑和肠道的损伤。

IF 1.7 4区医学

Neurological Research Pub Date : 2024-11-01 Epub Date: 2024-07-30 DOI: 10.1080/01616412.2024.2381160

Wei Zhao, Ruzhi Li, Xiang Zhong, Peizan Huang

引用次数: 0