Nature Communications最新文献

{"title":"Arabidopsis response to the apocarotenoid zaxinone involves interference with strigolactone signaling via binding to DWARF14.","authors":"Juan C Moreno,Umar F Shahul Hameed,Aparna Balakrishna,Abdugaffor Ablazov,Kit Xi Liew,Muhammad Jamil,Jianing Mi,Kawthar Alashoor,Alexandre de Saint Germain,Stefan T Arold,Salim Al-Babili","doi":"10.1038/s41467-025-63845-w","DOIUrl":"https://doi.org/10.1038/s41467-025-63845-w","url":null,"abstract":"The natural growth regulator zaxinone increases the levels of the phytohormones strigolactone (SL) and abscisic acid in Arabidopsis (Arabidopsis thaliana) via unknown mechanisms. Here we demonstrate that part of the effect of zaxinone in Arabidopsis depend on the SL receptor DWARF14 (AtD14) and the F-Box protein MORE AXILLARY BRANCHING2 (AtMAX2) that mediate the signaling of SLs and karrikins. Binding assays and co-crystallization reveal zaxinone as an additional ligand of AtD14 and an SL antagonist that interrupts the interaction of AtD14 with AtMAX2. Zaxinone also binds to the karrikin receptor KARRIKIN INSENSITIVE2 (AtKAI2). These findings unveil a perception mechanism for zaxinone in Arabidopsis and demonstrate the capability of AtD14 to bind signaling molecules, other than strigolactones and mediate their transduction.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"100 1","pages":"8789"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and stable n-type sulfide overall water splitting with separated hydrogen production.","authors":"Haolin Luo,Zhixi Liu,Haifeng Lv,Junie Jhon M Vequizo,Mengting Zheng,Feng Han,Zhen Ye,Akira Yamakata,Wenfeng Shangguan,Adam F Lee,Xiaojun Wu,Domen Kazunari,Jun Lu,Zhi Jiang","doi":"10.1038/s41467-025-63840-1","DOIUrl":"https://doi.org/10.1038/s41467-025-63840-1","url":null,"abstract":"N-type sulfide semiconductors are promising photocatalysts due to their broad visible-light absorption, facile synthesis and chemical diversity. However, photocorrosion and limited electron transport in one-step excitation and solid-state Z-scheme systems hinder efficient overall water splitting. Liquid-phase Z-schemes offer a viable alternative, but sluggish mediator kinetics and interfacial side reactions impede their construction. Here we report a stable Z-scheme system integrating n-type CdS and BiVO₄ with a [Fe(CN)₆]³⁻/[Fe(CN)₆]⁴⁻ mediator, achieving 10.2% apparent quantum yield at 450 nm with stoichiometric H₂/O₂ evolution. High activity reflects synergies between Pt@CrOx and Co3O4 cocatalysts on CdS, and cobalt-directed facet asymmetry in BiVO₄, resulting in matched kinetics for hydrogen and oxygen evolution in a reversible mediator solution. Stability is dramatically improved through coating CdS and BiVO4 with different oxides to inhibit Fe4[Fe(CN)6]3 precipitation and deactivation by a hitherto unrecognized mechanism. Separate hydrogen and oxygen production is also demonstrated in a two-compartment reactor under visible light and ambient conditions. This work unlocks the long-sought potential of n-type sulfides for efficient, durable and safe solar-driven hydrogen production.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"10 1","pages":"8786"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicenter evaluation of label-free quantification in human plasma on a high dynamic range benchmark set.","authors":"Ute Distler,Han Byul Yoo,Oliver Kardell,Dana Hein,Malte Sielaff,Marian Scherer,Anna M Jozefowicz,Christian Leps,David Gomez-Zepeda,Christine von Toerne,Juliane Merl-Pham,Teresa K Barth,Johanna Tüshaus,Pieter Giesbertz,Torsten Müller,Georg Kliewer,Karim Aljakouch,Barbara Helm,Henry Unger,Dario L Frey,Dominic Helm,Luisa Schwarzmüller,Oliver Popp,Di Qin,Susanne I Wudy,Ludwig Roman Sinn,Julia Mergner,Christina Ludwig,Axel Imhof,Bernhard Kuster,Stefan F Lichtenthaler,Jeroen Krijgsveld,Ursula Klingmüller,Philipp Mertins,Fabian Coscia,Markus Ralser,Michael Mülleder,Stefanie M Hauck,Stefan Tenzer","doi":"10.1038/s41467-025-64501-z","DOIUrl":"https://doi.org/10.1038/s41467-025-64501-z","url":null,"abstract":"Human plasma is routinely collected during clinical care and constitutes a rich source of biomarkers for diagnostics and patient stratification. Liquid chromatography-mass spectrometry (LC-MS)-based proteomics is a key method for plasma biomarker discovery, but the high dynamic range of plasma proteins poses significant challenges for MS analysis and data processing. To benchmark the quantitative performance of neat plasma analysis, we introduce a multispecies sample set based on a human tryptic plasma digest containing varying low level spike-ins of yeast and E. coli tryptic proteome digests, termed PYE. By analysing the sample set on state-of-the-art LC-MS platforms across twelve different sites in data-dependent (DDA) and data-independent acquisition (DIA) modes, we provide a data resource comprising a total of 1116 individual LC-MS runs. Centralized data analysis shows that DIA methods outperform DDA-based approaches regarding identifications, data completeness, accuracy, and precision. DIA achieves excellent technical reproducibility, as demonstrated by coefficients of variation (CVs) between 3.3% and 9.8% at protein level. Comparative analysis of different setups clearly shows a high overlap in identified proteins and proves that accurate and precise quantitative measurements are feasible across multiple sites, even in a complex matrix such as plasma, using state-of-the-art instrumentation. The collected dataset, including the PYE sample set and strategy presented, serves as a valuable resource for optimizing the accuracy and reproducibility of LC-MS and bioinformatic workflows for clinical plasma proteome analysis.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"98 1","pages":"8774"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.","authors":"Eduard M Unterauer,Eva-Maria Schentarra,Isabelle Pachmayr,Taisha Tashrin,Jisoo Kwon,Sebastian Strauss,Kristina Jevdokimenko,Rafal Kowalewski,Felipe Opazo,Eugenio F Fornasiero,Luciano A Masullo,Ralf Jungmann","doi":"10.1038/s41467-025-64228-x","DOIUrl":"https://doi.org/10.1038/s41467-025-64228-x","url":null,"abstract":"Multiplexed super-resolution microscopy enables spatial proteomics at single-protein resolution, but current methods often depend on secondary labels, complicating implementation and limiting throughput. We introduce a streamlined approach that combines speed-optimized DNA-PAINT sequences with their mirror-image analogs (left-handed DNA), enabling rapid and efficient 12-plex imaging. Validated on synthetic and cellular benchmarks, our method maps dense neuronal interactomes in 3D with 15 nm spatial resolution across a 200 × 200 µm2 field of view.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"114 1","pages":"8773"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation-free artificial photosynthesis of concentrated hydrogen peroxide and value-added fuels over Ta atomic sites.","authors":"Hao Tan,Peng Zhou,Yu Gu,Youxing Liu,Wenxing Chen,Hongyu Guo,Fangxu Lin,Heng Luo,Xiaoqing Cao,Lingyou Zeng,Mingchuan Luo,Shaojun Guo","doi":"10.1038/s41467-025-63838-9","DOIUrl":"https://doi.org/10.1038/s41467-025-63838-9","url":null,"abstract":"Solar-driven artificial photosynthesis of hydrogen peroxide and high-value chemicals from oxygen and biomass is promising but is hindered by poor light absorption, sluggish kinetics of biomass dehydrogenation, and low oxygen reduction selectivity. Herein, we develop a resorcinol-formaldehyde resin/carbon-supported Ta-N2O2 single-atom catalyst (RF/C-TaSA) that enables broad-spectrum light harvesting (> 932 nm) and selective biomass conversion in a solid-organic-aqueous three-phase system. RF/C-TaSA achieves a high 3.0% quantum yield at 635 nm, with Ta-N2O2 sites stabilizing intermediates and reducing the energy barrier for biomass conversion. We further demonstrate that RF/C-TaSA enables efficient artificial photosynthesis of H2O2 and value-added chemicals from more available thatch, pine needles and wastepaper. Combining catalyst innovation and system engineering, we build a solar-powered RF/C-TaSA-based photocatalytic device for directly producing commercially viable H2O2 at a high concentration of 3 wt% and high-value-added chemical without requiring energy-intensive separation processes for 70-day operation. Furthermore, the produced crude concentrated H2O2 can also be readily converted to solid H2O2 powder (Na2CO3 ∙ 1.5 H2O2) for ease of storage and transport with high sterilization activities even after 6 months.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"10 1","pages":"8784"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tectonic-astronomical interactions in shaping late Paleozoic climate and organic carbon burial.","authors":"Ren Wei,Zhijun Jin,Mingsong Li,Shuai Yuan,Yongyun Hu,Lin Dong,Rui Zhang,Juye Shi","doi":"10.1038/s41467-025-63896-z","DOIUrl":"https://doi.org/10.1038/s41467-025-63896-z","url":null,"abstract":"Tectonic processes and astronomical cycles are key drivers of Earth's climate and carbon systems. However, their interplay in shaping late Paleozoic climate variability remains poorly constrained. Here, we divide the late Paleozoic (~360-250 Ma) into three distinct tectonic phases based on full-plate tectonic reconstructions, geochemical datasets, and carbon cycle modeling, thereby elucidating how global sea levels and organic carbon burial responded to astronomically forced climate fluctuations under different tectonic phases. Our results show that intervals spanning ~360-330 Ma and ~280-250 Ma were characterized by elevated atmospheric CO2 levels and intensified tectonic activity, which coincided with heightened climate variability and reduced regularity in orbitally paced sea-level changes. In contrast, during ~330-280 Ma, multiple proxies indicate reduced tectonic forcing and lower CO2 concentrations, which were accompanied by more stable climate conditions and clearer expression of astronomical cycles. These conditions facilitated rhythmic deposition and widespread organic carbon burial.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"102 1","pages":"8805"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet activation plays a pro-inflammatory role in myasthenia gravis.","authors":"Qi Wen, Shu Zhang, Yaye Wang, Haoran Liu, Jingsi Wang, Shengyao Su, Nairong Xie, Yan Lu, Li Di, Min Xu, Min Wang, Hai Chen, Suobin Wang, Wenjia Zhu, Xinmei Wen, Jinming Han, Dongshan Wan, Shufang Zhao, Wanting Lu, Zhen Tao, Jianying Duo, Yue Huang, Guoliang Chai, Ruisheng Duan, Xiaoli Li, Junwei Hao, Yuwei Da","doi":"10.1038/s41467-025-63750-2","DOIUrl":"https://doi.org/10.1038/s41467-025-63750-2","url":null,"abstract":"<p><p>Myasthenia gravis (MG) is an autoimmune disorder that disrupts neuromuscular junction function through autoantibodies. Platelets are emerging as key players in the pathogenesis of MG, bridging innate and adaptive immunity. We analyze platelet transcriptome signatures and their interactions with the immune system in AChR+ immunotherapy-naïve MG (nMG) patients using bulk and single-cell RNA sequencing on peripheral blood mononuclear cells (PBMC). nMG patients exhibit upregulation of genes related to activation, inflammation, and cytoskeletal regulation. Increased platelet count, activation, altered morphology, enhanced CD62P expression, and elevated plasma CD40L levels are observed in PBMCs, which diminish with minimal clinical status (MMS). Functionally, platelets show heightened interactions with leukocytes, forming aggregates that correlate with disease severity. These features return to baseline after intravenous immunoglobulin or prolonged immunosuppressive therapy. This study underscores platelet activation's critical role in MG and supports platelet-targeted therapy.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"16 1","pages":"8779"},"PeriodicalIF":15.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pincer-cobalt boosts divergent alkene carbonylation under tandem electro-thermo-catalysis.","authors":"Shulei Ge, Zhili Cui, Lei Peng, Xintong Wang, Kaixin Chen, Changrui Nie, Shoucheng Dong, Yang Huang, Gen Luo, Lin He, Jie Li","doi":"10.1038/s41467-025-63875-4","DOIUrl":"https://doi.org/10.1038/s41467-025-63875-4","url":null,"abstract":"<p><p>Catalytic multicomponent carbonylation reactions with high regio- and chemoselectivity represent one of the long-pursued goals in C1 chemistry. We herein disclose a practical cobalt-catalyzed divergent radical alkene carbonylative functionalization under 1 atm of CO at 23 °C. The leverage of the tridentate NNN-type pincer ligand is the key to avoid the formation of catalytically inert Co⁰(CO)_n species and overcome the occurrence of oxidative carbonylation of organozincs, selectively tuning the catalytic reactivity of cobalt center for dictating a full cobalt-catalyzed four-component carbonylation. Moreover, direct use CO₂ as the C1 source in the multicomponent alkene carbonylative couplings can be achieved under a tandem electro-thermo-catalysis, thus allowing us to rapidly and reliably construct unsymmetric ketones with ample scope and excellent functional group compatibility. Remarkably, our protocol encompasses a broader of polyhaloalkanes as the electrophiles, which underwent radical-relay couplings in a completely regio- and chemoselective fashion. Finally, facile modifications of drug-like molecules demonstrate the synthetic utility of this method.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"16 1","pages":"8803"},"PeriodicalIF":15.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring changes in vitamin D levels during the COVID-19 pandemic with routinely-collected laboratory data.","authors":"Lea Skapetze, Daniela Koller, Andreas Zwergal, Stefan Feuerriegel, Anna Rubinski, Eva Grill","doi":"10.1038/s41467-025-64192-6","DOIUrl":"https://doi.org/10.1038/s41467-025-64192-6","url":null,"abstract":"<p><p>Vitamin D is critical for bone health and immune function, but the COVID-19 pandemic, characterized by lockdowns and reduced outdoor activity, raised concerns about potential declines in vitamin D levels due to dietary changes and limited sunlight exposure. In this study, we analyzed routinely-collected laboratory data (N = 292,187 patients) from a large laboratory chain in Bavaria, Germany, to assess changes in vitamin D levels before (March 2018 to February 2020) and during (March 2020 to February 2022) the pandemic. Different statistical approaches (i.e., descriptive statistics, propensity score matching, and a causal forest) were used to evaluate confounder-adjusted changes in vitamin D levels and deficiency rates. Mean vitamin D levels decreased significantly from 26.7 μg/l pre-pandemic to 26.0 μg/l during the pandemic (p-value < 0.001), with a corresponding increase in deficiency rates from 31.2% to 35.2% (p-value < 0.001). Across all statistical approaches, the decline in mean levels and the increase in deficiency rates were particularly pronounced among elderly women. These findings highlight the importance of public health strategies to monitor and improve vitamin D status, especially during periods of restricted outdoor activity.</p>","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"16 1","pages":"8772"},"PeriodicalIF":15.7,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orbital and millennial-scale forcing of the Patagonian Ice Sheet throughout the Last Glacial Cycle.","authors":"Andrés Castillo-Llarena,Matthias Prange,Irina Rogozhina","doi":"10.1038/s41467-025-64614-5","DOIUrl":"https://doi.org/10.1038/s41467-025-64614-5","url":null,"abstract":"During the Last Glacial Maximum (23,000 to 19,000 years ago), the Patagonian Ice Sheet covered the central chain of the Andes between 38 °S and 55 °S. The paleoclimatic evidence from Patagonia and New Zealand suggests that the maximum glacier expansion of the Southern Hemisphere mid-latitudes was desynchronized with the Northern Hemisphere glacial history. Here we present numerical simulations of the Patagonian ice sheet throughout the Last Glacial Cycle. Our analysis suggests that the Patagonian ice sheet had two main periods of advance, during the Marine Isotope Stage 4 and late Marine Isotope Stage 3, experiencing inter-millennial scale variability. We show that the Patagonian Ice Sheet long-term evolution can be attributed to changes in the integrated summer insolation, which combines the summer duration and insolation intensity and has an obliquity-like periodicity. We further suggest that this metric also modulated the behaviour of glaciers over the entire Southern Hemisphere mid-latitudes.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"98 1","pages":"8776"},"PeriodicalIF":16.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}