拟南芥对拟南芥类伪胡萝卜素zaxinone的反应包括通过与DWARF14结合干扰独角麦内酯信号传导。

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-02 DOI:10.1038/s41467-025-63845-w

Juan C Moreno,Umar F Shahul Hameed,Aparna Balakrishna,Abdugaffor Ablazov,Kit Xi Liew,Muhammad Jamil,Jianing Mi,Kawthar Alashoor,Alexandre de Saint Germain,Stefan T Arold,Salim Al-Babili

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引用次数: 0

摘要

天然生长调节剂zaxinone通过未知的机制增加拟南芥（Arabidopsis thaliana）植物激素独角麦内酯（SL）和脱落酸的水平。本研究表明，zaxinone在拟南芥中的部分作用依赖于SL受体DWARF14 （AtD14）和F-Box蛋白MORE AXILLARY BRANCHING2 (AtMAX2)，它们介导SLs和卡瑞金斯的信号传导。结合实验和共结晶表明，zaxinone是AtD14的附加配体和SL拮抗剂，可阻断AtD14与AtMAX2的相互作用。Zaxinone还与karrikin受体AtKAI2结合。这些发现揭示了拟南芥中zaxinone的感知机制，并证明了AtD14结合除独脚金内酯外的信号分子并介导其转导的能力。

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Arabidopsis response to the apocarotenoid zaxinone involves interference with strigolactone signaling via binding to DWARF14.

The natural growth regulator zaxinone increases the levels of the phytohormones strigolactone (SL) and abscisic acid in Arabidopsis (Arabidopsis thaliana) via unknown mechanisms. Here we demonstrate that part of the effect of zaxinone in Arabidopsis depend on the SL receptor DWARF14 (AtD14) and the F-Box protein MORE AXILLARY BRANCHING2 (AtMAX2) that mediate the signaling of SLs and karrikins. Binding assays and co-crystallization reveal zaxinone as an additional ligand of AtD14 and an SL antagonist that interrupts the interaction of AtD14 with AtMAX2. Zaxinone also binds to the karrikin receptor KARRIKIN INSENSITIVE2 (AtKAI2). These findings unveil a perception mechanism for zaxinone in Arabidopsis and demonstrate the capability of AtD14 to bind signaling molecules, other than strigolactones and mediate their transduction.

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.