Nature Communications最新文献

Environmental cues in different host niches shape the survival fitness of Staphylococcus aureus 不同宿主生态位的环境因素影响金黄色葡萄球菌的生存适应性

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-62292-x

JuOae Chang, ChaeYoung Lee, Inseo Kim, Jihyeon Kim, Ji-Hoon Kim, Taegwan Yun, David C. Hooper, Suzanne Walker, Wonsik Lee

{"title":"Environmental cues in different host niches shape the survival fitness of Staphylococcus aureus","authors":"JuOae Chang, ChaeYoung Lee, Inseo Kim, Jihyeon Kim, Ji-Hoon Kim, Taegwan Yun, David C. Hooper, Suzanne Walker, Wonsik Lee","doi":"10.1038/s41467-025-62292-x","DOIUrl":"https://doi.org/10.1038/s41467-025-62292-x","url":null,"abstract":"The ability of Staphylococcus aureus to adapt and thrive in diverse host niches adds to the challenge in combating this ubiquitous pathogen. While extensive research has been pursued on the adaptive mechanisms of methicillin-resistant S. aureus (MRSA) in various infection models, a comprehensive analysis of its fitness across different host niches is lacking. In this study, we employ transposon sequencing to analyze the adaptive strategies of MRSA in various infection niches. Our analysis encompasses a cell model that mimics an intracellular niche, human blood, which represents a major extracellular environment as well as a major intermediary route encountered by bacteria during systemic infection, and a male murine sepsis model that recapitulates intra-organ environments. Our findings reveal substantial differences in the genetic determinants essential for bacterial survival in intracellular and blood environments. Moreover, we show that each organ imposes unique growth constraints, thus fostering heterogeneity within the mutant population that can enter and survive in each organ of the mouse. By comparing genes important for survival across all examined host environments, we identify 27 core genes that represent potential therapeutic targets for treating S. aureus infections. Additionally, our findings aid in understanding how bacteria adapt to diverse host environments.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"4 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution DynaTag用于在低输入样品和单细胞分辨率下高效定位转录因子

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-61797-9

Pascal Hunold, Giulia Pizzolato, Nadia Heramvand, Laura Kaiser, Giulia Barbiera, Olivia van Ray, Roman Thomas, Julie George, Martin Peifer, Robert Hänsel-Hertsch

{"title":"DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution","authors":"Pascal Hunold, Giulia Pizzolato, Nadia Heramvand, Laura Kaiser, Giulia Barbiera, Olivia van Ray, Roman Thomas, Julie George, Martin Peifer, Robert Hänsel-Hertsch","doi":"10.1038/s41467-025-61797-9","DOIUrl":"https://doi.org/10.1038/s41467-025-61797-9","url":null,"abstract":"Systematic discovery of transcription factor (TF) landscapes in low-input samples and at single cell level is a major challenge in the fields of molecular biology, genetics, and epigenetics. Here, we present cleavage under Dynamic targets and Tagmentation (DynaTag), enabling robust mapping of TF-DNA interactions using a physiological salt solution during sample preparation. DynaTag uncovers occupancy alterations for 15 TFs in stem cell and cancer tissue models. We highlight changes in TF-DNA binding for NANOG, MYC, and OCT4, during stem-cell differentiation, at both bulk and single-cell resolutions. DynaTag surpasses CUT&RUN and ChIP-seq in signal-to-background ratio and resolution. Furthermore, using tumours of a small cell lung cancer model derived from a single female donor, DynaTag reveals increased chromatin occupancy of FOXA1, MYC, and the mutant p53 R248Q at enriched gene pathways (e.g. epithelial-mesenchymal transition), following chemotherapy treatment. Collectively, we believe that DynaTag represents a significant technological advancement, facilitating precise characterization of TF landscapes across diverse biological systems and complex models.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"27 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron triggers grain boundary structural transformation in steel 硼触发钢的晶界组织转变

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-62264-1

Xuyang Zhou, Sourabh Kumar, Siyuan Zhang, Xinren Chen, Baptiste Gault, Gerhard Dehm, Tilmann Hickel, Dierk Raabe

{"title":"Boron triggers grain boundary structural transformation in steel","authors":"Xuyang Zhou, Sourabh Kumar, Siyuan Zhang, Xinren Chen, Baptiste Gault, Gerhard Dehm, Tilmann Hickel, Dierk Raabe","doi":"10.1038/s41467-025-62264-1","DOIUrl":"https://doi.org/10.1038/s41467-025-62264-1","url":null,"abstract":"Boron enhances the hardenability of low-alloyed steel and reduces embrittlement at low temperatures, at parts-per-million concentration levels. Its effectiveness arises from segregation to grain boundaries (GBs)-planar defects between crystals-yet atomic-scale evidence remains limited. We addressed this gap by synthesizing GBs with controllable geometry and orientation, enabling reproducible comparison with and without boron segregation. Differential phase-contrast imaging directly reveals boron at iron GBs, and in-situ TEM heating (20 °C to 800 °C) allows us to track the dynamic evolution of GB structures. We found that boron segregation induces local structural changes and triggers GB phase transformations, as corroborated by calculated GB defect phase diagrams spanning broad ranges of carbon and boron content. Our findings not only bridge a gap in understanding the interplay between GB structure and chemistry but also lay the groundwork for targeted design and passivation strategies in steel, potentially transforming its resistance to hydrogen embrittlement, corrosion, and mechanical failure.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"12 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A central and unified role of corticocortical feedback in parsing visual scenes 皮质-皮质反馈在解析视觉场景中的中心和统一作用

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-62279-8

Ye Xin, Yin Yan, Wu Li

{"title":"A central and unified role of corticocortical feedback in parsing visual scenes","authors":"Ye Xin, Yin Yan, Wu Li","doi":"10.1038/s41467-025-62279-8","DOIUrl":"https://doi.org/10.1038/s41467-025-62279-8","url":null,"abstract":"Visual scene analysis in the brain involves diverse grouping and segmentation processes mediated by intra- and inter-areal interactions, but the role of feedback from higher to lower visual cortices has remained largely speculative, relying mostly on indirect evidence. In this study with behaving monkeys, we investigated the causal impact of V4-to-V1 feedback on different figure-ground perceptual tasks employing distinct contextual cues. We compared neuronal responses in the primary visual cortex (V1) and the behavioral performance before and after silencing a higher-order visual area (V4). Our results reveal that V4-to-V1 feedback comprises dissociable facilitatory and inhibitory components that differ in their spatial distribution, onset time, modulatory polarity, and magnitude. This feedback is essential for, and consistent across, distinct grouping and segmentation processes, operating independently of V1 neurons’ selectivity for local features and primarily modifying the late phases of neuronal responses. These findings highlight fundamental differences between the mechanisms underlying global, feature-independent figure-ground organization and local, feature-dependent contextual analysis. Moreover, while the feedback modifies correlated variability in neural responses, this effect does not alter the neural population code. Our study underscores the central and unified role of corticocortical feedback in integrating and interpreting visual scenes, with implications for circuit-level mechanisms.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"22 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4f-modified Ru-O polarity as a descriptor for efficient electrocatalytic acidic oxygen evolution 4f修饰的Ru-O极性作为高效电催化酸性析氧的描述符

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-62258-z

Xiuxiu Zhang, Yuhao Zhang, Bogdan O. Protsenko, Mikhail A. Soldatov, Jing Zhang, Chenyu Yang, Shuowen Bo, Huijuan Wang, Xin Chen, Chao Wang, Weiren Cheng, Qinghua Liu

{"title":"4f-modified Ru-O polarity as a descriptor for efficient electrocatalytic acidic oxygen evolution","authors":"Xiuxiu Zhang, Yuhao Zhang, Bogdan O. Protsenko, Mikhail A. Soldatov, Jing Zhang, Chenyu Yang, Shuowen Bo, Huijuan Wang, Xin Chen, Chao Wang, Weiren Cheng, Qinghua Liu","doi":"10.1038/s41467-025-62258-z","DOIUrl":"https://doi.org/10.1038/s41467-025-62258-z","url":null,"abstract":"The development of non-iridium-based oxygen evolution reaction (OER) catalysts is crucial for proton exchange membrane water electrolysis (PEMWE), but hydrogen production remains a great challenge because of sluggish OER kinetics and severe catalyst dissolution. Here, we present a 4f-induced covalent polarity modulation strategy for the construction of 4f-orbital-modified RuO2 (4f-RuO2) nanocatalysts with tunable Ru–O polarity. We find that the OER activity of 4f-RuO2 shows a volcano shape as a function of the polarity of Ru–O bond. Consequently, the best 4f-Nd-RuO2 catalyst possesses an ultra-low overpotential of 214 mV at 10 mA cm−2 and robust electrochemical stability in 0.1 M HClO4. Theoretical calculations coupled with in situ synchrotron infrared and X-ray absorption spectroscopy analyses reveal that the modulation of Ru–O polarity in RuO2 by the valence f−p−d gradient orbital coupling can modify the adsorption energy of the reaction intermediates and suppress the participation of lattice oxygen to avoid over-oxidation of Ru, which can thus serve as an effective descriptor for fine tuning the activity and durability of acidic OER nanocatalysts.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"27 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dedicated skin-to-brain circuit for cool sensation in mice 一个专门用于小鼠凉爽感觉的皮肤-大脑回路

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-61562-y

Hankyu Lee, Chia Chun Hor, Lorraine R. Horwitz, Ailin Xiong, Xin-Yu Su, Daniel R. Soden, Sarah Yang, Wei Cai, Wenwen Zhang, Chen Li, Christopher Radcliff, Abbey Dinh, Tin Long Rex Fung, Ilma Rovcanin, Kevin P. Pipe, X. Z. Shawn Xu, Bo Duan

{"title":"A dedicated skin-to-brain circuit for cool sensation in mice","authors":"Hankyu Lee, Chia Chun Hor, Lorraine R. Horwitz, Ailin Xiong, Xin-Yu Su, Daniel R. Soden, Sarah Yang, Wei Cai, Wenwen Zhang, Chen Li, Christopher Radcliff, Abbey Dinh, Tin Long Rex Fung, Ilma Rovcanin, Kevin P. Pipe, X. Z. Shawn Xu, Bo Duan","doi":"10.1038/s41467-025-61562-y","DOIUrl":"https://doi.org/10.1038/s41467-025-61562-y","url":null,"abstract":"Perception of external temperature is essential for maintaining homeostasis and avoiding thermal injury. Although molecular thermosensors such as transient receptor potential melastatin type 8 (TRPM8) have been identified, the neural circuits responsible for transmitting cool signals remain unclear. Here we show that a spinal circuit in mice conveys cool signals from the skin to the brain. Excitatory interneurons in the spinal dorsal horn expressing thyrotropin-releasing hormone receptor (Trhr+) act as a central hub for cool sensation. These Trhr+ neurons receive monosynaptic input from TRPM8+ sensory afferents and are selectively activated by innocuous cool stimuli. Ablating Trhr+ interneurons abolishes behavioral responses to cool, but not to warm or cold stimuli. We also identify a population of calcitonin receptor-like receptor-positive (Calcrl+) spinal projection neurons that receive convergent input from both TRPM8+ afferents and Trhr+ interneurons, and transmit cool-specific signals to the lateral parabrachial nucleus (lPBN). Our findings define a feedforward amplification circuit for cool sensation and reveal a modality-specific spinal pathway for thermal processing.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"214 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyborg insect factory: automatic assembly for insect-computer hybrid robot via vision-guided robotic arm manipulation of custom bipolar electrodes 半机械人昆虫工厂：通过视觉引导机械臂操纵定制双极电极，自动组装昆虫-计算机混合机器人

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-60779-1

Qifeng Lin, Nghia Vuong, Kewei Song, Phuoc Thanh Tran-Ngoc, Greg Angelo Gonzales Nonato, Hirotaka Sato

{"title":"Cyborg insect factory: automatic assembly for insect-computer hybrid robot via vision-guided robotic arm manipulation of custom bipolar electrodes","authors":"Qifeng Lin, Nghia Vuong, Kewei Song, Phuoc Thanh Tran-Ngoc, Greg Angelo Gonzales Nonato, Hirotaka Sato","doi":"10.1038/s41467-025-60779-1","DOIUrl":"https://doi.org/10.1038/s41467-025-60779-1","url":null,"abstract":"Insect–computer hybrid robots offer strong potential for navigating complex terrains. This study identified the intersegmental membrane between the pronotum and mesothorax of the Madagascar hissing cockroach as an effective site for electrical stimulation to control direction and speed. A pair of bipolar electrodes was custom-designed, and an automatic assembly system was developed, integrating a robotic arm, vision-based site detection, and an insect fixation structure. The system achieved assembly in 68 s. Hybrid robots exhibited robust steering (over 70°) and deceleration (68.2% speed reduction) with performance comparable to manually assembled counterparts. Controlled navigation along an S-shaped path confirmed accurate directional control. Furthermore, a multi-agent system of four hybrid robots covered 80.25% of an obstructed terrain in 10 minutes and 31 seconds. This work demonstrates a scalable strategy for automating the fabrication of insect–computer hybrid robots, enabling efficient and reproducible assembly process while maintaining effective locomotion control.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"22 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cationic alkyl chain length and nanoaggregate form of ionic liquids dominate biocompatibility and toxicity 阳离子烷基链长度和纳米聚集体形式决定了离子液体的生物相容性和毒性

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-62206-x

Yuyuan Xing, Yanhui Hu, Xiao Zhang, Diwei Zheng, Guanghui Ma, Yanyan Diao, Hua Yue, Wei Wei, Suojiang Zhang

{"title":"Cationic alkyl chain length and nanoaggregate form of ionic liquids dominate biocompatibility and toxicity","authors":"Yuyuan Xing, Yanhui Hu, Xiao Zhang, Diwei Zheng, Guanghui Ma, Yanyan Diao, Hua Yue, Wei Wei, Suojiang Zhang","doi":"10.1038/s41467-025-62206-x","DOIUrl":"https://doi.org/10.1038/s41467-025-62206-x","url":null,"abstract":"While ionic liquids (ILs) have diverse applications, their potential in biomedical applications remains largely untapped due to gaps in systematic understanding of the spectrum of IL biosafety (biocompatibility/toxicity). Here, we establish an IL library and identify an in vitro reduction in biocompatibility (increased toxicity) with increased ILs’ cationic alkyl chain length. Particularly, we present compelling evidence for IL nanoaggregates in aqueous environment, thereby elucidating the mechanisms involved in cell interactions. ILs with short cationic alkyl chains (scILs) are restricted in intracellular vesicles, whereas ILs with long cationic alkyl chains (lcILs) accumulate to the mitochondria for inducing mitophagy and apoptosis. The occurrence of dysfunctional behaviour in lcILs is also observed in vivo, with a positive correlation between the lcIL signal in tissues and mitophagy/apoptotic levels. Irrespective of the administration routes (oral/intramuscular/intravenous), scILs exhibit ~30–80 times greater tolerance than lcILs. The feasibility of scIL nanoaggregates as carriers for insoluble drugs is thus validated, and an enhanced bioavailability over the commercial tablet is acquired. The findings obtained by integrating computational analysis with diverse cell/animal evaluations (from multiple cell lines, cell spheroids, patient-derived organoids to male murine and canine models) offer unique insights into the behaviour, mechanisms, and biomedical application scenarios of IL nanoaggregates.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"144 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of HDAC6 alters fumarate hydratase activity and mitochondrial structure 抑制HDAC6可改变富马酸水合酶活性和线粒体结构

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-61897-6

Andrew Roe, Catríona M. Dowling, Cian D’Arcy, Daniel Alencar Rodrigues, Yu Wang, Matthew Hiller, Carl Keogh, Kate E. R. Hollinshead, Massimiliano Garre, Brenton Cavanagh, Kieran Wynne, Tianyan Liu, Zhixing Chen, Emma Kerr, Marie McIlroy, Jochen H. M. Prehn, Ingmar Schoen, Tríona Ní Chonghaile

{"title":"Inhibition of HDAC6 alters fumarate hydratase activity and mitochondrial structure","authors":"Andrew Roe, Catríona M. Dowling, Cian D’Arcy, Daniel Alencar Rodrigues, Yu Wang, Matthew Hiller, Carl Keogh, Kate E. R. Hollinshead, Massimiliano Garre, Brenton Cavanagh, Kieran Wynne, Tianyan Liu, Zhixing Chen, Emma Kerr, Marie McIlroy, Jochen H. M. Prehn, Ingmar Schoen, Tríona Ní Chonghaile","doi":"10.1038/s41467-025-61897-6","DOIUrl":"https://doi.org/10.1038/s41467-025-61897-6","url":null,"abstract":"Fumarate hydratase (FH), a key node of mitochondrial metabolism, is also a tumour suppressor. Despite its prominent roles in tumourigenesis and inflammation, its regulation remains poorly understood. Herein, we show that histone deacetylase 6 (HDAC6) regulates FH activity. In triple-negative breast cancer cells, HDAC6 inhibition or knockdown results in alterations to mitochondrial cristae structure, as detected by live-cell super-resolution STED nanoscopy and electron microscopy, along with the release of mitochondrial DNA. Mass-spectrometry immunoprecipitation reveals multiple mitochondrial HDAC6-interactors, with FH emerging as a top hit. Super-resolution 3D-STORM shows HDAC6 interactions with FH in mitochondrial networks, which increases after perturbation of HDAC6 activity with BAS-2. Treatment with BAS-2 leads to fumarate accumulation by 13C glucose labelling, along with downstream succination of proteins and cell death. Together, these results identify HDAC6 inhibition as a regulator of endogenous FH activity in tumour cells, and highlight it as a promising candidate for indirectly targeting tumour metabolism.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"12 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical control of vascular morphogenesis by the surrounding stiffness 周围刚度对血管形态发生的生物力学控制

IF 16.6 1区综合性期刊

Nature Communications Pub Date : 2025-07-28 DOI: 10.1038/s41467-025-61804-z

Yasuyuki Hanada, Semanti Halder, Yuichiro Arima, Misato Haruta, Honami Ogoh, Shuntaro Ogura, Yukihiko Shiraki, Sota Nakano, Yuka Ozeki, Shigetomo Fukuhara, Akiyoshi Uemura, Toyoaki Murohara, Koichi Nishiyama

{"title":"Biomechanical control of vascular morphogenesis by the surrounding stiffness","authors":"Yasuyuki Hanada, Semanti Halder, Yuichiro Arima, Misato Haruta, Honami Ogoh, Shuntaro Ogura, Yukihiko Shiraki, Sota Nakano, Yuka Ozeki, Shigetomo Fukuhara, Akiyoshi Uemura, Toyoaki Murohara, Koichi Nishiyama","doi":"10.1038/s41467-025-61804-z","DOIUrl":"https://doi.org/10.1038/s41467-025-61804-z","url":null,"abstract":"Sprouting angiogenesis is a form of morphogenesis which expands vascular networks from preexisting networks. However, the precise mechanism governing efficient branch elongation driven by directional movement of endothelial cells (ECs), while the lumen develops under the influence of blood inflow, remains unknown. Herein, we show perivascular stiffening to be a major factor that integrates branch elongation and lumen development. The lumen expansion seen during lumen development inhibits directional EC movement driving branch elongation. This process is counter-regulated by the presence of pericytes, which induces perivascular stiffening by promoting the deposition of EC-derived collagen-IV (Col-IV) on the vascular basement membrane (VBM), thereby preventing excessive lumen expansion. Furthermore, inhibition of forward directional movement of the tip EC during lumen development is associated with decreased localization of the F-BAR proteins and Arp2/3 complexes at the leading front. Our results demonstrate how ECs elongate branches, while the lumen develops, by properly building the surrounding physical environment in coordination with pericytes during angiogenesis.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"20 1","pages":""},"PeriodicalIF":16.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0