左手DNA在单蛋白分辨率下高效高复用成像。

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-02 DOI:10.1038/s41467-025-64228-x

Eduard M Unterauer,Eva-Maria Schentarra,Isabelle Pachmayr,Taisha Tashrin,Jisoo Kwon,Sebastian Strauss,Kristina Jevdokimenko,Rafal Kowalewski,Felipe Opazo,Eugenio F Fornasiero,Luciano A Masullo,Ralf Jungmann

{"title":"左手DNA在单蛋白分辨率下高效高复用成像。","authors":"Eduard M Unterauer,Eva-Maria Schentarra,Isabelle Pachmayr,Taisha Tashrin,Jisoo Kwon,Sebastian Strauss,Kristina Jevdokimenko,Rafal Kowalewski,Felipe Opazo,Eugenio F Fornasiero,Luciano A Masullo,Ralf Jungmann","doi":"10.1038/s41467-025-64228-x","DOIUrl":null,"url":null,"abstract":"Multiplexed super-resolution microscopy enables spatial proteomics at single-protein resolution, but current methods often depend on secondary labels, complicating implementation and limiting throughput. We introduce a streamlined approach that combines speed-optimized DNA-PAINT sequences with their mirror-image analogs (left-handed DNA), enabling rapid and efficient 12-plex imaging. Validated on synthetic and cellular benchmarks, our method maps dense neuronal interactomes in 3D with 15 nm spatial resolution across a 200 × 200 µm2 field of view.","PeriodicalId":19066,"journal":{"name":"Nature Communications","volume":"114 1","pages":"8773"},"PeriodicalIF":15.7000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.\",\"authors\":\"Eduard M Unterauer,Eva-Maria Schentarra,Isabelle Pachmayr,Taisha Tashrin,Jisoo Kwon,Sebastian Strauss,Kristina Jevdokimenko,Rafal Kowalewski,Felipe Opazo,Eugenio F Fornasiero,Luciano A Masullo,Ralf Jungmann\",\"doi\":\"10.1038/s41467-025-64228-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Multiplexed super-resolution microscopy enables spatial proteomics at single-protein resolution, but current methods often depend on secondary labels, complicating implementation and limiting throughput. We introduce a streamlined approach that combines speed-optimized DNA-PAINT sequences with their mirror-image analogs (left-handed DNA), enabling rapid and efficient 12-plex imaging. Validated on synthetic and cellular benchmarks, our method maps dense neuronal interactomes in 3D with 15 nm spatial resolution across a 200 × 200 µm2 field of view.\",\"PeriodicalId\":19066,\"journal\":{\"name\":\"Nature Communications\",\"volume\":\"114 1\",\"pages\":\"8773\"},\"PeriodicalIF\":15.7000,\"publicationDate\":\"2025-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature Communications\",\"FirstCategoryId\":\"103\",\"ListUrlMain\":\"https://doi.org/10.1038/s41467-025-64228-x\",\"RegionNum\":1,\"RegionCategory\":\"综合性期刊\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MULTIDISCIPLINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Communications","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41467-025-64228-x","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

多路超分辨率显微镜使空间蛋白质组学在单蛋白分辨率，但目前的方法往往依赖于二级标签，复杂的实施和限制吞吐量。我们介绍了一种简化的方法，将速度优化的DNA- paint序列与其镜像类似物（左旋DNA）相结合，实现快速高效的12路成像。经过合成和细胞基准测试的验证，我们的方法在200 × 200µm2的视场中以15 nm的空间分辨率在3D中绘制密集的神经元相互作用组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Left-handed DNA for efficient highly multiplexed imaging at single-protein resolution.

Multiplexed super-resolution microscopy enables spatial proteomics at single-protein resolution, but current methods often depend on secondary labels, complicating implementation and limiting throughput. We introduce a streamlined approach that combines speed-optimized DNA-PAINT sequences with their mirror-image analogs (left-handed DNA), enabling rapid and efficient 12-plex imaging. Validated on synthetic and cellular benchmarks, our method maps dense neuronal interactomes in 3D with 15 nm spatial resolution across a 200 × 200 µm2 field of view.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.