Molecular Biology Research Communications最新文献_第3页

Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters. 利用自体转运体的 C 端结构域在大肠杆菌表面表达红色荧光蛋白。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.49860.1956

Khoi-Nguyen Le-Hoang, Thanh-Tan Nguyen, Hieu Tran-Van

{"title":"Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters.","authors":"Khoi-Nguyen Le-Hoang, Thanh-Tan Nguyen, Hieu Tran-Van","doi":"10.22099/mbrc.2024.49860.1956","DOIUrl":"10.22099/mbrc.2024.49860.1956","url":null,"abstract":"The Type V secretion system, or \"autotransporter\", is a secretion system that enables bacteria to directly export proteins from the cell interior to the extracellular membrane. mCherry is a second-generation monomeric red fluorescent protein that has an improvement in photostability compared to the first generation of RFP. In this research, we conducted the fusion of the mRFP into the C-terminal domain of EhaA - the translocation domain of the autotransporter protein transport system - to investigate the expression of mRFP on the surface of Escherichia coli , a model organism commonly utilized in recombinant protein research. The induction of the mRFP-EhaA C-terminal domain complex expression was achieved using isopropyl β-D-1-thiogalactopyranoside (IPTG) and confirmed through SDS-PAGE stained with Coomassie Brilliant Blue and Western blotting using anti-6X His tag antibodies. The surface expression of the mRFP-EhaA C-terminal complex protein was validated through the fluorescent properties of mRFP and further confirmed using fluorescent microscopy. This study laid the groundwork for surface expression on cost-effective Gram-negative bacteria, E. coli.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"31-35"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico exploring of the epigenetic factors in teratozoospermia: A focus on IGF2BP2. 畸形精子症表观遗传因素的芯片探索：以IGF2BP2为重点。

IF 1

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.52777.2123

Seyedeh Zahra Mousavi, Bahram Mohammad-Soltani, Morteza Hadizadeh, Zeynab Rokhsattalab, Mehdi Totonchi

{"title":"In silico exploring of the epigenetic factors in teratozoospermia: A focus on IGF2BP2.","authors":"Seyedeh Zahra Mousavi, Bahram Mohammad-Soltani, Morteza Hadizadeh, Zeynab Rokhsattalab, Mehdi Totonchi","doi":"10.22099/mbrc.2025.52777.2123","DOIUrl":"10.22099/mbrc.2025.52777.2123","url":null,"abstract":"Teratozoospermia is an abnormal sperm morphology that is a common cause of male infertility. Epigenetic factors have been implicated in the regulation of gene expression in teratozoospermia, but the specific mechanisms are not fully understood. This study aimed to identify differentially expressed genes (DEGs) between teratozoospermia and normozoospermia samples, and to investigate the role of epigenetic regulatory factors in the observed gene expression changes. The study integrated data from three publicly available datasets in the GSE6969 superseries. The DEGs were compared to a list of known human epigenetic-related genes obtained from the EpiFactors database. The protein-protein interaction (PPI) network and hub gene identifications for Epi-DEGs and the RNA-protein interaction (RPI) network to obtained the RBPs interacting with Epi-DEGs were constructed. siRNA design for the candidate mRNA was performed using various bioinformatics tools. As a result, the obtained 1,292 DEGs were compared to a list of 796 known human epigenetic factors, revealing 63 Epi-DEGs. The PPI network of Epi-DEGs identified top 10 hub genes including RBBP7, HDAC2, EZH2, SMARCA5, SUV39H1, CTCF, DNMT1, MORF4L1, ARID4B and KDM5B. The RPI network analysis revealed IGF2BP2, SFPQ and A1CF as key RNA-binding protein regulators epigenetic modifiers. Based on these findings, the study designed the sequence GCAACAAGAGAAGAA GCAATT as an optimal siRNA candidate targeting the master regulator IGF2BP2, which exhibited the most significant change in expression among the RNA-binding proteins (RBPs). This integrative analysis sheds light on the epigenetic mechanisms underlying teratozoospermia and highlights the potential of RBPs as diagnostic biomarkers and therapeutic targets for further investigation.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 4","pages":"271-281"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPIN.DOC induces cellular transformation of NIH3T3 normal mouse fibroblast cells. SPIN.DOC诱导NIH3T3正常小鼠成纤维细胞的细胞转化。

IF 1

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.52821.2125

Khuraijam Mrinalini Devi, Thangjam Davis Singh, Rubismita Deka, Lisam Shanjukumar Singh, Thiyam Ramsing Singh

引用次数: 0

Dysregulated LINC01133 expression in laryngeal carcinoma: Prognostic implications and predicted ceRNA interactome. 喉癌中LINC01133表达异常：预后意义和预测ceRNA相互作用组。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50390.1996

Masoumeh Razipour, Zeinab Jamali, Saeed Sohrabpour, Farrokh Heidari, Maryam Lotfi, Elham Ghadami, Maryam Abtin, Mohaddese Maghsudlu, Leyla Sahebi, Abbas Shakoori

{"title":"Dysregulated LINC01133 expression in laryngeal carcinoma: Prognostic implications and predicted ceRNA interactome.","authors":"Masoumeh Razipour, Zeinab Jamali, Saeed Sohrabpour, Farrokh Heidari, Maryam Lotfi, Elham Ghadami, Maryam Abtin, Mohaddese Maghsudlu, Leyla Sahebi, Abbas Shakoori","doi":"10.22099/mbrc.2024.50390.1996","DOIUrl":"10.22099/mbrc.2024.50390.1996","url":null,"abstract":"Long non-coding RNAs (lncRNAs) have recently emerged as critical regulators of oncogenic or tumor-suppressive pathways in human cancers. LINC01133 is a lncRNA that has exhibited dichotomous roles in various malignancies but to the best of our knowledge, the role of LINC01133 in laryngeal squamous cell carcinoma (LSCC) has not been previously investigated. This study aimed to investigate the expression, clinical significance, and potential functions of the LINC01133 in LSCC. Integrative bioinformatics analysis of sequencing data obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets revealed LINC01133 as a differentially expressed lncRNA in head and neck/laryngeal cancers. Experimental validation via quantitative real-time PCR in 41 pairs of stage III and IV LSCC tissues and normal tissues adjacent to the tumor (NAT) demonstrated significant downregulation of LINC01133 in tumors (p<0.0001). Decreased LINC01133 expression associated with advanced tumor stage (p=0.0206) and lymph node metastasis (p=0.0203). The receiver operating characteristic analysis indicated potential diagnostic utility (AUC=0.7115, p=0.001). Bioinformatic predictions and literature mining suggested two potential competing endogenous RNA (ceRNA) mechanisms whereby LINC01133 may act as a tumor suppressor by sponging miR-205-5p to derepress the leucine-rich repeat kinase 2 (LRRK2) and androgen receptor, leading to dysregulation of cancer-related signaling cascades. This study provides initial evidence that loss of lncRNA LINC01133 expression may promote LSCC tumorigenesis, possibly by dysregulating microRNA interactions. Further verification of its regulatory mechanisms and diagnostic value is warranted.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"93-107"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-spike protein inhibition of SARS-CoV-2 by natural products through the key mediator protein ORF8. 天然产物通过关键中介蛋白ORF8对SARS-CoV-2的非刺突蛋白抑制作用

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50245.2001

Mostafa Bagheri-Far, Mohammad Assadizadeh, Maryam Azimzadeh-Irani, Mohammad Yaghoubi-Avini, Seyed Massoud Hosseini

{"title":"Non-spike protein inhibition of SARS-CoV-2 by natural products through the key mediator protein ORF8.","authors":"Mostafa Bagheri-Far, Mohammad Assadizadeh, Maryam Azimzadeh-Irani, Mohammad Yaghoubi-Avini, Seyed Massoud Hosseini","doi":"10.22099/mbrc.2024.50245.2001","DOIUrl":"10.22099/mbrc.2024.50245.2001","url":null,"abstract":"The recent pernicious COVID-19 pandemic is caused by SARS-CoV-2. While most therapeutic strategies have focused on the viral spike protein, Open Reading Frame 8 (ORF8) plays a critical role in causing the severity of the disease. Nonetheless, there still needs to be more information on the ORF8 binding epitopes and their appropriate safe inhibitors. Herein, the protein binding sites were detected through comprehensive structural analyses. The validation of the binding sites was investigated through protein conservation analysis and blind docking. The potential natural product (NP) inhibitors were selected based on a structure-function approach. The solo and combined inhibition functions of these NPs were examined through molecular docking studies. Two binding epitopes were identified, one between the ORF8 monomers (DGBM) and the other on the surface (Gal1-Like). E92 was predicted to be pivotal for DGBM, and R101 for Gal1-like, which was then confirmed through molecular dockings. The inhibitory effects of selected phytochemical (Artemisinin), bacterial (Ivermectin), and native-liken (DEG-168) NPs were compared with the Remdesivir. Selected NPs showed solo- and co-functionality against Remdesivir to inhibit functional regions of the ORF8 structure. The DGBM is highly engaged in capturing the NPs. Additionally, the co-functionality study of NPs showed that the Ivermectin-DEG168 combination has the strongest mechanism for inhibiting all the predicted binding sites. Ivermectin can interfere with ORF8-MHC-I interaction through inhibition of A51 and F120. Two new binding sites on this non-infusion protein structure were introduced using a combination of approaches. Additionally, three safe and effective were found to inhibit these binding sites.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"73-91"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of 30-day Ramadan fasting on autophagy pathway and metabolic health outcome in healthy individuals. 斋月禁食30天对健康个体自噬途径和代谢健康结局的影响

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50105.1978

Sanaz Dastghaib, Morvarid Siri, Nasim Rahmani-Kukia, Seyed Taghi Heydari, Mehdi Pasalar, Mozhdeh Zamani, Pooneh Mokaram, Kamran Bagheri-Lankarani

{"title":"Effect of 30-day Ramadan fasting on autophagy pathway and metabolic health outcome in healthy individuals.","authors":"Sanaz Dastghaib, Morvarid Siri, Nasim Rahmani-Kukia, Seyed Taghi Heydari, Mehdi Pasalar, Mozhdeh Zamani, Pooneh Mokaram, Kamran Bagheri-Lankarani","doi":"10.22099/mbrc.2024.50105.1978","DOIUrl":"https://doi.org/10.22099/mbrc.2024.50105.1978","url":null,"abstract":"During Ramadan, Muslims fast to spiritually prepare their bodies and spirits. The autophagy pathway restores cellular homeostasis and is being studied as a therapy for a variety of disorders. According to previous studies, fasting or calorie restriction has a role in the up-regulation of autophagy especially through the initiation step. The effects of Ramadan fasting on the autophagy pathways and metabolic health outcome in healthy adults were investigated in this study. In this controlled cohort study, 50 healthy subjects (20-78 years old) 24-fasting and 26 non-fasting were included. At the end of Ramadan, a blood was drawn to assess biochemical, hematological, and inflammatory variables. Serum IL-6 and hs-CRP levels were measured. The serum proteins (Beclin-1 and LC3β) and mRNAs gene expressions' (Beclin-1, p62, and LC3β) of the autophagy marker were evaluated by ELISA and real-time PCR, respectively. During Ramadan, there were no significant differences for biochemical parameters (except for BUN-level), inflammatory markers (IL-6 and hs-CRP), and hematological indices during Ramadan. Beclin-1 gene expression was significantly upregulated in the fasting-group as the main marker of initiation of autophagy; yet, the LC3β and the p62 levels were decreased in the fasting-group in peripheral blood mononuclear cells. However, fasting women alone displayed a significantly high serum Beclin-1 level. Ramadan fasting does not have any adverse effects on biochemical, hematological, and inflammatory parameters. According to our results, people observing Ramadan may benefit from the autophagy pathway to compensate reduction in energy and vital metabolites in the face of food restriction.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"115-127"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of chemotherapeutic agents on epidermal neural crest stem cells. 化疗药物对表皮神经嵴干细胞的影响。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.49755.1948

Nasim Rahmani-Kukia, Fatemeh Keshavarzi, Mohammad Saied Salehi, Farzaneh Bozorg-Ghalati, Zahra Mojtahedi, Mozhdeh Zamani, Negar Azarpira, Pooneh Mokarram

{"title":"The effect of chemotherapeutic agents on epidermal neural crest stem cells.","authors":"Nasim Rahmani-Kukia, Fatemeh Keshavarzi, Mohammad Saied Salehi, Farzaneh Bozorg-Ghalati, Zahra Mojtahedi, Mozhdeh Zamani, Negar Azarpira, Pooneh Mokarram","doi":"10.22099/mbrc.2024.49755.1948","DOIUrl":"https://doi.org/10.22099/mbrc.2024.49755.1948","url":null,"abstract":"Human Epidermal Neural Crest Stem Cells (hEPI-NCSCs), as a transient population of multipotent migratory stem cells can differentiate into multiple types of neural and non-neural cells and tissues in the body. Here, we tried to determine the role of chemo agents in mediating the stress induced pathways like autophagy and unfolded protein responses (UPR), as well as the migratory potential of NCSCs. hEPI-NCSCs were treated with chemo agents including Dithiothreitol [(DTT) 10µM)], Salinomycin (9mM), Ebselen (10mM), 5-Fluorouracil [(5-FU) 8µM] and Cisplatin (6mM) for 72 hours. The reverse transcription-quantitative polymerase chain reaction (RT- qPCR) and scratch wound healing assays were used to assess the effect of chemo agents on NCSCs function. After treatment with DTT, hEPI-NCSCs upregulated the expression of genes related to autophagy and UPR pathways including LC3, P62 and CHOP. These genes were also overexpressed when NCSCs were treated with Salinomycin. Reverse results were verified by 5-FU, Ebselen and Cisplatin treatment. Salinomycin and Cisplatin upregulated the expression of XBP-1, which down regulated with DTT, 5-FU and Ebselen. Inhibition in migratory capacity of NCSCs was detected following treatment by Salinomycin, 5-FU, Ebselen and Cisplatin. DTT and 5-FU promoted the expression of BDNF, while Salinomycin, Cisplatin and Ebselen treatment reduced its expression. During exposition to DTT, the autophagy pathway was activated, implying that autophagy functions as a survival mechanism for deactivating the inhibitory effects of DTT on the migratory capacity of NCSCs. Chemotherapeutic agents like 5-FU and cisplatin exert cytotoxic effects on NCSCs by suppressing autophagy, UPR pathways, and the migratory potential of NCSCs.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"167-175"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenic variants of BUB1 and BUBR1 genes are not prioritized in screening tests of couples with aborted aneuploid fetuses. 在对流产的非整倍体胎儿的夫妇进行筛选试验时，不会优先考虑BUB1和BUBR1基因的致病变异。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.51170.2037

Raziyeh Gorji, Parnaz Borjian-Boroujeni, Masood Bazrgar

{"title":"Pathogenic variants of BUB1 and BUBR1 genes are not prioritized in screening tests of couples with aborted aneuploid fetuses.","authors":"Raziyeh Gorji, Parnaz Borjian-Boroujeni, Masood Bazrgar","doi":"10.22099/mbrc.2024.51170.2037","DOIUrl":"https://doi.org/10.22099/mbrc.2024.51170.2037","url":null,"abstract":"Chromosome aberrations certainly aneuploidie are the cause of the majority of spontaneous abortions in humans. BUB1 (budding uninhibited by benzimidazole 1) and BUBR1 (BUB1 mitotic checkpoint serine/threonine kinase B) are two key proteins mediating spindle-checkpoint activation that play a role in the inhibition of the anaphase-promoting complex/ cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. This study aimed to evaluate the probable roles of BUB1 and BUBR1 pathogenic variants in abortion of the fetuses with aneuploidy. Fifty aborted fetuses with approved aneuploidy using array comparative genomic hybridization (aCGH) were included. BUB1 and BUBR1 genes were studied using the Sanger sequencing for the single nucleotide variant (SNV) detection, certainly rs121909055 and rs28989185 as the pathogenic target variants. The sequencing results were analyzed by finch TV software.Neither homozygous nor heterozygous variant of the targeted SNVs was observed in the samples. No other SNV was detectable in the analyzed parts of the BUB1 and BUBR1 genes in all samples. Since the allele frequencies of the variants of interest were zero in 50 studied samples, these SNVs would not be prioritized for screening in the parents with a history of miscarriage due to aneuploidy.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"143-147"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of VOPP1 in regulation of Paclitaxel response and EMT process during ovarian tumor progression. 卵巢肿瘤进展过程中VOPP1在紫杉醇反应和EMT过程中的调控作用。

IF 1

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.52515.2107

Mohammad Hossein Khajavirad, Amirhosein Maharati, Negin Taghehchian, Fatemeh Taghavinia, Meysam Moghbeli

{"title":"Role of VOPP1 in regulation of Paclitaxel response and EMT process during ovarian tumor progression.","authors":"Mohammad Hossein Khajavirad, Amirhosein Maharati, Negin Taghehchian, Fatemeh Taghavinia, Meysam Moghbeli","doi":"10.22099/mbrc.2025.52515.2107","DOIUrl":"10.22099/mbrc.2025.52515.2107","url":null,"abstract":"Ovarian cancer (OC) is one of the most common malignancies of the genitourinary system in women that has a high mortality rate worldwide. Drug resistance and tumor relapse are the main causes of high mortality rate in OC patients. Therefore, investigation of the molecular mechanisms involved in OC progression can be valuable to introduce effective therapeutic targets for these patients. Epithelial-mesenchymal transition (EMT) as a key regulator of tumor relapse and drug resistance can be regulated by different signaling pathways such as WNT and NOTCH. VOPP1 is an activator of NF-κB pathway during tumor progression. Considering the importance of cross talks between different signaling pathways during tumor progression, we assessed the role of VOPP1 in OC progression through the modulation of WNT and NOTCH pathways. The expression levels of components of WNT and NOTCH signaling pathways, as well as the EMT process, were evaluated in VOPP1-induced A2780 cells compared to control cells. Role of VOPP1 in OC invasiveness was also assessed through migration and drug resistance assays. VOPP1 inhibited EMT process and NOTCH and WNT pathways in A2780 cells. VOPP1 also significantly reduced cell migration (p=0.04) and paclitaxel (PTX) resistance in A2780 cells (p<0.0001). VOPP1 reduced ovarian tumor cell migration and PTX resistance via regulation of NOTCH and WNT mediated EMT process. Therefore, it can be suggested as a novel therapeutic target in OC patients following further animal studies and clinical trials.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 4","pages":"249-258"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12426957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico structural analysis of Heterocephalus glaber amyloid beta: an anti-Alzheimer's peptide. 褐藻淀粉样蛋白 beta：一种抗老年痴呆症肽的分子内结构分析。

IF 1.6

Molecular Biology Research Communications Pub Date : 2024-01-01 DOI: 10.22099/mbrc.2023.48223.1862

Ali Javanmard, Maryam Azimzadeh-Irani, Ghazal Tafazzoli, Ayla Esmaeilzadeh, Mohammad Shirinpoor-Kharf, Seyyed Mohammad Hasan Haghayeghi

{"title":"In-silico structural analysis of Heterocephalus glaber amyloid beta: an anti-Alzheimer's peptide.","authors":"Ali Javanmard, Maryam Azimzadeh-Irani, Ghazal Tafazzoli, Ayla Esmaeilzadeh, Mohammad Shirinpoor-Kharf, Seyyed Mohammad Hasan Haghayeghi","doi":"10.22099/mbrc.2023.48223.1862","DOIUrl":"10.22099/mbrc.2023.48223.1862","url":null,"abstract":"Heterocephalus glaber, known as the Naked mole-rat, has an extraordinary immunity to Alzheimer's disease. The pathological hallmark of Alzheimer's disease is cerebral accumulations of plaques, consisting of self-aggregated amyloid beta peptides. Homo sapiens and H. glaber amyloid beta peptides are different in only one amino acid. Herein, computational structural analyses were carried out to determine whether plaque development in H. glaber is prevented by the replacement of His13 with Arg13 in the amyloid beta peptide. AlphaFold2 was used to predict the structure of the H. glaber amyloid beta peptide. HADDOCK and Hex were used to self-dock the peptides and dock ions on peptides, respectively. Illustrations were made by PyMol and ChimeraX. Using VMD, we calculated the radius of gyration. The phylogenetic analysis was conducted by Mega. The results showed an accurate structure with two alpha helices separated by a short coil for H. glaber. Self-docking of the two amyloid beta peptides demonstrated a globular conformation in the H. glaber dimer, implying the unlikeliness of amyloid beta peptides' self-aggregation to form fibrillar structures. This conformational state resulted in lower electrostatic energy compared to H. sapiens, contributing to H. glaber's lower tendency for fibril and, ultimately, plaque formation. Phylogenetic analysis confirmed that amyloid precursor protein is highly conserved in each taxon of rodentia and primata. This study provides insight into the connection between the structure of H. glaber amyloid beta and its plaque formation properties, showing that the Arg13 in H. glaber leads to fibril instability, and might prevent senile plaque accumulation.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"13 1","pages":"29-42"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0