Molecular Biology Research Communications最新文献_第2页

Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions. 分离耐高温碳氢化合物降解细菌并确定其特征，这些细菌在极端盐度和高渗透条件下仍能保持活性。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.49747.1946

Saman Hosseini, Rouhallah Sharifi, Alireza Habibi, Ali Beheshti-AleAgha

{"title":"Isolation and characterization of thermotolerant hydrocarbon degrading bacteria which sustained the activity at extreme salinity and high osmotic conditions.","authors":"Saman Hosseini, Rouhallah Sharifi, Alireza Habibi, Ali Beheshti-AleAgha","doi":"10.22099/mbrc.2024.49747.1946","DOIUrl":"10.22099/mbrc.2024.49747.1946","url":null,"abstract":"The bioremediation method is considered an economical and environmentally friendly strategy for the remediation of oil-contaminated soils. However, some oil field areas have extreme environmental conditions that make it difficult to establish microbes for bioreme-diation. In this study, bacteria were isolated from oil-contaminated soils of the Dehloran oil fields, which have very harsh soil and weather conditions. Soil samples were collected from two highly contaminated mud pits. The petroleum content and physicochemical characteristics of the soil were investigated. Soil samples pollution were about 8%, sandy and alkaline, and their EC reached up to 125.6 ds/m in some samples. The isolated bacteria were screened according to their ability to grow on the M9 mineral medium containing crude oil as the sole carbon source. Moreover, their physiological characteristics in diesel degradation were investigated. The phenotypic, biochemical, and molecular characteristics of selected isolates and their stability under extreme conditions such as drought, salinity and high temperatures were investigated. Two isolates NC39 and NB391 showed the highest ability in diesel degradation. The results of 16SrRNA sequencing showed that NC39 isolate had 98% similarity to Pseudomonas sp. and isolate NB391 belonged to Pantoea agglomerans with 99% similarity. These two isolates showed a high ability to tolerate high salinity (10%), temperature (50°C), and drought (-0.73 MPa) stress. Exploiting these extremophile strains is a promising tool in the bioremediation of oil-contaminated soils in extreme environments.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"37-46"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters. 利用自体转运体的 C 端结构域在大肠杆菌表面表达红色荧光蛋白。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.49860.1956

Khoi-Nguyen Le-Hoang, Thanh-Tan Nguyen, Hieu Tran-Van

{"title":"Expressing red fluorescent protein on the surface of Escherichia coli using C-terminal domain of autotransporters.","authors":"Khoi-Nguyen Le-Hoang, Thanh-Tan Nguyen, Hieu Tran-Van","doi":"10.22099/mbrc.2024.49860.1956","DOIUrl":"10.22099/mbrc.2024.49860.1956","url":null,"abstract":"The Type V secretion system, or \"autotransporter\", is a secretion system that enables bacteria to directly export proteins from the cell interior to the extracellular membrane. mCherry is a second-generation monomeric red fluorescent protein that has an improvement in photostability compared to the first generation of RFP. In this research, we conducted the fusion of the mRFP into the C-terminal domain of EhaA - the translocation domain of the autotransporter protein transport system - to investigate the expression of mRFP on the surface of Escherichia coli , a model organism commonly utilized in recombinant protein research. The induction of the mRFP-EhaA C-terminal domain complex expression was achieved using isopropyl β-D-1-thiogalactopyranoside (IPTG) and confirmed through SDS-PAGE stained with Coomassie Brilliant Blue and Western blotting using anti-6X His tag antibodies. The surface expression of the mRFP-EhaA C-terminal complex protein was validated through the fluorescent properties of mRFP and further confirmed using fluorescent microscopy. This study laid the groundwork for surface expression on cost-effective Gram-negative bacteria, E. coli.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"31-35"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulated LINC01133 expression in laryngeal carcinoma: Prognostic implications and predicted ceRNA interactome. 喉癌中LINC01133表达异常：预后意义和预测ceRNA相互作用组。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50390.1996

Masoumeh Razipour, Zeinab Jamali, Saeed Sohrabpour, Farrokh Heidari, Maryam Lotfi, Elham Ghadami, Maryam Abtin, Mohaddese Maghsudlu, Leyla Sahebi, Abbas Shakoori

{"title":"Dysregulated LINC01133 expression in laryngeal carcinoma: Prognostic implications and predicted ceRNA interactome.","authors":"Masoumeh Razipour, Zeinab Jamali, Saeed Sohrabpour, Farrokh Heidari, Maryam Lotfi, Elham Ghadami, Maryam Abtin, Mohaddese Maghsudlu, Leyla Sahebi, Abbas Shakoori","doi":"10.22099/mbrc.2024.50390.1996","DOIUrl":"10.22099/mbrc.2024.50390.1996","url":null,"abstract":"Long non-coding RNAs (lncRNAs) have recently emerged as critical regulators of oncogenic or tumor-suppressive pathways in human cancers. LINC01133 is a lncRNA that has exhibited dichotomous roles in various malignancies but to the best of our knowledge, the role of LINC01133 in laryngeal squamous cell carcinoma (LSCC) has not been previously investigated. This study aimed to investigate the expression, clinical significance, and potential functions of the LINC01133 in LSCC. Integrative bioinformatics analysis of sequencing data obtained from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets revealed LINC01133 as a differentially expressed lncRNA in head and neck/laryngeal cancers. Experimental validation via quantitative real-time PCR in 41 pairs of stage III and IV LSCC tissues and normal tissues adjacent to the tumor (NAT) demonstrated significant downregulation of LINC01133 in tumors (p<0.0001). Decreased LINC01133 expression associated with advanced tumor stage (p=0.0206) and lymph node metastasis (p=0.0203). The receiver operating characteristic analysis indicated potential diagnostic utility (AUC=0.7115, p=0.001). Bioinformatic predictions and literature mining suggested two potential competing endogenous RNA (ceRNA) mechanisms whereby LINC01133 may act as a tumor suppressor by sponging miR-205-5p to derepress the leucine-rich repeat kinase 2 (LRRK2) and androgen receptor, leading to dysregulation of cancer-related signaling cascades. This study provides initial evidence that loss of lncRNA LINC01133 expression may promote LSCC tumorigenesis, possibly by dysregulating microRNA interactions. Further verification of its regulatory mechanisms and diagnostic value is warranted.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"93-107"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-spike protein inhibition of SARS-CoV-2 by natural products through the key mediator protein ORF8. 天然产物通过关键中介蛋白ORF8对SARS-CoV-2的非刺突蛋白抑制作用

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50245.2001

Mostafa Bagheri-Far, Mohammad Assadizadeh, Maryam Azimzadeh-Irani, Mohammad Yaghoubi-Avini, Seyed Massoud Hosseini

{"title":"Non-spike protein inhibition of SARS-CoV-2 by natural products through the key mediator protein ORF8.","authors":"Mostafa Bagheri-Far, Mohammad Assadizadeh, Maryam Azimzadeh-Irani, Mohammad Yaghoubi-Avini, Seyed Massoud Hosseini","doi":"10.22099/mbrc.2024.50245.2001","DOIUrl":"10.22099/mbrc.2024.50245.2001","url":null,"abstract":"The recent pernicious COVID-19 pandemic is caused by SARS-CoV-2. While most therapeutic strategies have focused on the viral spike protein, Open Reading Frame 8 (ORF8) plays a critical role in causing the severity of the disease. Nonetheless, there still needs to be more information on the ORF8 binding epitopes and their appropriate safe inhibitors. Herein, the protein binding sites were detected through comprehensive structural analyses. The validation of the binding sites was investigated through protein conservation analysis and blind docking. The potential natural product (NP) inhibitors were selected based on a structure-function approach. The solo and combined inhibition functions of these NPs were examined through molecular docking studies. Two binding epitopes were identified, one between the ORF8 monomers (DGBM) and the other on the surface (Gal1-Like). E92 was predicted to be pivotal for DGBM, and R101 for Gal1-like, which was then confirmed through molecular dockings. The inhibitory effects of selected phytochemical (Artemisinin), bacterial (Ivermectin), and native-liken (DEG-168) NPs were compared with the Remdesivir. Selected NPs showed solo- and co-functionality against Remdesivir to inhibit functional regions of the ORF8 structure. The DGBM is highly engaged in capturing the NPs. Additionally, the co-functionality study of NPs showed that the Ivermectin-DEG168 combination has the strongest mechanism for inhibiting all the predicted binding sites. Ivermectin can interfere with ORF8-MHC-I interaction through inhibition of A51 and F120. Two new binding sites on this non-infusion protein structure were introduced using a combination of approaches. Additionally, three safe and effective were found to inhibit these binding sites.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"73-91"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of chemotherapeutic agents on epidermal neural crest stem cells.

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.49755.1948

Nasim Rahmani-Kukia, Fatemeh Keshavarzi, Mohammad Saied Salehi, Farzaneh Bozorg-Ghalati, Zahra Mojtahedi, Mozhdeh Zamani, Negar Azarpira, Pooneh Mokarram

{"title":"The effect of chemotherapeutic agents on epidermal neural crest stem cells.","authors":"Nasim Rahmani-Kukia, Fatemeh Keshavarzi, Mohammad Saied Salehi, Farzaneh Bozorg-Ghalati, Zahra Mojtahedi, Mozhdeh Zamani, Negar Azarpira, Pooneh Mokarram","doi":"10.22099/mbrc.2024.49755.1948","DOIUrl":"https://doi.org/10.22099/mbrc.2024.49755.1948","url":null,"abstract":"Human Epidermal Neural Crest Stem Cells (hEPI-NCSCs), as a transient population of multipotent migratory stem cells can differentiate into multiple types of neural and non-neural cells and tissues in the body. Here, we tried to determine the role of chemo agents in mediating the stress induced pathways like autophagy and unfolded protein responses (UPR), as well as the migratory potential of NCSCs. hEPI-NCSCs were treated with chemo agents including Dithiothreitol [(DTT) 10µM)], Salinomycin (9mM), Ebselen (10mM), 5-Fluorouracil [(5-FU) 8µM] and Cisplatin (6mM) for 72 hours. The reverse transcription-quantitative polymerase chain reaction (RT- qPCR) and scratch wound healing assays were used to assess the effect of chemo agents on NCSCs function. After treatment with DTT, hEPI-NCSCs upregulated the expression of genes related to autophagy and UPR pathways including LC3, P62 and CHOP. These genes were also overexpressed when NCSCs were treated with Salinomycin. Reverse results were verified by 5-FU, Ebselen and Cisplatin treatment. Salinomycin and Cisplatin upregulated the expression of XBP-1, which down regulated with DTT, 5-FU and Ebselen. Inhibition in migratory capacity of NCSCs was detected following treatment by Salinomycin, 5-FU, Ebselen and Cisplatin. DTT and 5-FU promoted the expression of BDNF, while Salinomycin, Cisplatin and Ebselen treatment reduced its expression. During exposition to DTT, the autophagy pathway was activated, implying that autophagy functions as a survival mechanism for deactivating the inhibitory effects of DTT on the migratory capacity of NCSCs. Chemotherapeutic agents like 5-FU and cisplatin exert cytotoxic effects on NCSCs by suppressing autophagy, UPR pathways, and the migratory potential of NCSCs.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"167-175"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of 30-day Ramadan fasting on autophagy pathway and metabolic health outcome in healthy individuals.

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50105.1978

Sanaz Dastghaib, Morvarid Siri, Nasim Rahmani-Kukia, Seyed Taghi Heydari, Mehdi Pasalar, Mozhdeh Zamani, Pooneh Mokaram, Kamran Bagheri-Lankarani

{"title":"Effect of 30-day Ramadan fasting on autophagy pathway and metabolic health outcome in healthy individuals.","authors":"Sanaz Dastghaib, Morvarid Siri, Nasim Rahmani-Kukia, Seyed Taghi Heydari, Mehdi Pasalar, Mozhdeh Zamani, Pooneh Mokaram, Kamran Bagheri-Lankarani","doi":"10.22099/mbrc.2024.50105.1978","DOIUrl":"https://doi.org/10.22099/mbrc.2024.50105.1978","url":null,"abstract":"During Ramadan, Muslims fast to spiritually prepare their bodies and spirits. The autophagy pathway restores cellular homeostasis and is being studied as a therapy for a variety of disorders. According to previous studies, fasting or calorie restriction has a role in the up-regulation of autophagy especially through the initiation step. The effects of Ramadan fasting on the autophagy pathways and metabolic health outcome in healthy adults were investigated in this study. In this controlled cohort study, 50 healthy subjects (20-78 years old) 24-fasting and 26 non-fasting were included. At the end of Ramadan, a blood was drawn to assess biochemical, hematological, and inflammatory variables. Serum IL-6 and hs-CRP levels were measured. The serum proteins (Beclin-1 and LC3β) and mRNAs gene expressions' (Beclin-1, p62, and LC3β) of the autophagy marker were evaluated by ELISA and real-time PCR, respectively. During Ramadan, there were no significant differences for biochemical parameters (except for BUN-level), inflammatory markers (IL-6 and hs-CRP), and hematological indices during Ramadan. Beclin-1 gene expression was significantly upregulated in the fasting-group as the main marker of initiation of autophagy; yet, the LC3β and the p62 levels were decreased in the fasting-group in peripheral blood mononuclear cells. However, fasting women alone displayed a significantly high serum Beclin-1 level. Ramadan fasting does not have any adverse effects on biochemical, hematological, and inflammatory parameters. According to our results, people observing Ramadan may benefit from the autophagy pathway to compensate reduction in energy and vital metabolites in the face of food restriction.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"115-127"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenic variants of BUB1 and BUBR1 genes are not prioritized in screening tests of couples with aborted aneuploid fetuses.

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.51170.2037

Raziyeh Gorji, Parnaz Borjian-Boroujeni, Masood Bazrgar

{"title":"Pathogenic variants of BUB1 and BUBR1 genes are not prioritized in screening tests of couples with aborted aneuploid fetuses.","authors":"Raziyeh Gorji, Parnaz Borjian-Boroujeni, Masood Bazrgar","doi":"10.22099/mbrc.2024.51170.2037","DOIUrl":"https://doi.org/10.22099/mbrc.2024.51170.2037","url":null,"abstract":"Chromosome aberrations certainly aneuploidie are the cause of the majority of spontaneous abortions in humans. BUB1 (budding uninhibited by benzimidazole 1) and BUBR1 (BUB1 mitotic checkpoint serine/threonine kinase B) are two key proteins mediating spindle-checkpoint activation that play a role in the inhibition of the anaphase-promoting complex/ cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. This study aimed to evaluate the probable roles of BUB1 and BUBR1 pathogenic variants in abortion of the fetuses with aneuploidy. Fifty aborted fetuses with approved aneuploidy using array comparative genomic hybridization (aCGH) were included. BUB1 and BUBR1 genes were studied using the Sanger sequencing for the single nucleotide variant (SNV) detection, certainly rs121909055 and rs28989185 as the pathogenic target variants. The sequencing results were analyzed by finch TV software.Neither homozygous nor heterozygous variant of the targeted SNVs was observed in the samples. No other SNV was detectable in the analyzed parts of the BUB1 and BUBR1 genes in all samples. Since the allele frequencies of the variants of interest were zero in 50 studied samples, these SNVs would not be prioritized for screening in the parents with a history of miscarriage due to aneuploidy.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"143-147"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico structural analysis of Heterocephalus glaber amyloid beta: an anti-Alzheimer's peptide. 褐藻淀粉样蛋白 beta：一种抗老年痴呆症肽的分子内结构分析。

IF 1.6

Molecular Biology Research Communications Pub Date : 2024-01-01 DOI: 10.22099/mbrc.2023.48223.1862

Ali Javanmard, Maryam Azimzadeh-Irani, Ghazal Tafazzoli, Ayla Esmaeilzadeh, Mohammad Shirinpoor-Kharf, Seyyed Mohammad Hasan Haghayeghi

{"title":"In-silico structural analysis of Heterocephalus glaber amyloid beta: an anti-Alzheimer's peptide.","authors":"Ali Javanmard, Maryam Azimzadeh-Irani, Ghazal Tafazzoli, Ayla Esmaeilzadeh, Mohammad Shirinpoor-Kharf, Seyyed Mohammad Hasan Haghayeghi","doi":"10.22099/mbrc.2023.48223.1862","DOIUrl":"10.22099/mbrc.2023.48223.1862","url":null,"abstract":"Heterocephalus glaber, known as the Naked mole-rat, has an extraordinary immunity to Alzheimer's disease. The pathological hallmark of Alzheimer's disease is cerebral accumulations of plaques, consisting of self-aggregated amyloid beta peptides. Homo sapiens and H. glaber amyloid beta peptides are different in only one amino acid. Herein, computational structural analyses were carried out to determine whether plaque development in H. glaber is prevented by the replacement of His13 with Arg13 in the amyloid beta peptide. AlphaFold2 was used to predict the structure of the H. glaber amyloid beta peptide. HADDOCK and Hex were used to self-dock the peptides and dock ions on peptides, respectively. Illustrations were made by PyMol and ChimeraX. Using VMD, we calculated the radius of gyration. The phylogenetic analysis was conducted by Mega. The results showed an accurate structure with two alpha helices separated by a short coil for H. glaber. Self-docking of the two amyloid beta peptides demonstrated a globular conformation in the H. glaber dimer, implying the unlikeliness of amyloid beta peptides' self-aggregation to form fibrillar structures. This conformational state resulted in lower electrostatic energy compared to H. sapiens, contributing to H. glaber's lower tendency for fibril and, ultimately, plaque formation. Phylogenetic analysis confirmed that amyloid precursor protein is highly conserved in each taxon of rodentia and primata. This study provides insight into the connection between the structure of H. glaber amyloid beta and its plaque formation properties, showing that the Arg13 in H. glaber leads to fibril instability, and might prevent senile plaque accumulation.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"13 1","pages":"29-42"},"PeriodicalIF":1.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10644309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulation of key regulatory factors in sphingosine-1-phosphate (S1P) pathway in human lung fibroblasts transfected with selected microRNAs. 转染特定 microRNAs 的人肺成纤维细胞中鞘磷脂-1-磷酸（S1P）通路关键调控因子的下调。

IF 1.5

Molecular Biology Research Communications Pub Date : 2024-01-01 DOI: 10.22099/mbrc.2024.49810.1951

Abdolamir Allameh, Mostafa Atashbasteh, Esmaeil Mortaz, Bahareh Naeeni, Majid Jafari-Khorchani

{"title":"Down-regulation of key regulatory factors in sphingosine-1-phosphate (S1P) pathway in human lung fibroblasts transfected with selected microRNAs.","authors":"Abdolamir Allameh, Mostafa Atashbasteh, Esmaeil Mortaz, Bahareh Naeeni, Majid Jafari-Khorchani","doi":"10.22099/mbrc.2024.49810.1951","DOIUrl":"10.22099/mbrc.2024.49810.1951","url":null,"abstract":"Sphingosine 1 phosphate (S1P) is involved in the pathogenesis of asthma by stimulation of the alpha-smooth muscle actin (SMA) expression and remodeling of fibroblasts. This study was designed to determine the effects of selected micro RNAs in regulation of S1P and related metabolic pathways in a human lung fibroblast cell line. The fibroblast cell line (CIRC-HLF, C580) was cultured and transfected with individual viral vectors carrying miR124, mi125b, mi133b or mi130a. After 48 hours, expression level of miRNAs and their target genes, sphingosine kinase 1(SPHK1), sphingosine 1-phosphate lyase 1 (SGPL1), sphingosine 1- phosphate receptor 1 (S1PR1) and sphingosine 1- phosphate receptor 2 (S1PR2) were determined. Expression of miRNA and mRNA determined by reverse transcriptionquantitative polymerase chain reaction (qPCR) showed that the expression level of the miRNAs was significantly higher in human lung fibroblasts following transfection compared to controls (vector backbone without miRNA). The expressions of miRNAs-targeted genes were significantly downregulated in transfected fibroblasts compared to control cells (p<0.05). Data show that miR 124, miR 125b, miR 133b and miR130a by targeting regulatory genes in S1P-pathway can down-regulate key factors such as SPHK1, SGPL1, S1PR1 and S1PR2 genes in lung fibroblasts. The results showed that S1P pathway and key factors are suppressed in lung fibroblasts expressing miR124, miR125b, miR130a or miR133b. It appears that suppression of any of the intermediate factors in S1P by miRNA can affect the regulation of the entire S1P pathway.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"13 4","pages":"201-209"},"PeriodicalIF":1.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11416850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Examining the expression of low-density lipoprotein receptor (LDLR) and low-density lipoprotein receptor-related protein 6 (LRP6) genes in breast cancer cell lines. 研究乳腺癌细胞系中低密度脂蛋白受体（LDLR）和低密度脂蛋白受体相关蛋白 6（LRP6）基因的表达。

IF 1.6

Molecular Biology Research Communications Pub Date : 2024-01-01 DOI: 10.22099/MBRC.2024.48583.1882

Hamid Behrouj, Mehran Erfani, Pooneh Mokarram

引用次数: 0