Molecular Biology Research Communications最新文献

Dysregulated genes in HIGK-treated F. nucleatum and their possible association with HNSCC. higk处理的核仁梭菌中基因失调及其与鳞状细胞癌的可能关联。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50171.1982

S B Shanmugam, J Vijayashree Priyadharsini, P Anitha, A S Smiline Girija, A Paramasivam

{"title":"Dysregulated genes in HIGK-treated F. nucleatum and their possible association with HNSCC.","authors":"S B Shanmugam, J Vijayashree Priyadharsini, P Anitha, A S Smiline Girija, A Paramasivam","doi":"10.22099/mbrc.2024.50171.1982","DOIUrl":"10.22099/mbrc.2024.50171.1982","url":null,"abstract":"The present study aims to identify the differentially expressed genes in HIGK treated with Fusobacterium nucleatum (Fn) and their possible role in establishing head and neck squamous cell carcinoma. The study design follows a computational approach wherein multiple databases and tools are used to derive the possible association between Fn exposure and the development of HNSCC. The GEOmnibus dataset GSE6927 provided data on the differentially expressed genes in the HIGK treated with Fn. The GEO2R analysis revealed 22 differentially expressed genes in HIGK cells treated with Fn. The expression profile of these genes was then analyzed in the HNSCC (TCGA, Firehose Legacy) dataset employing the UALCAN database. The present study revealed 5 genes viz., GSDMD, NUP214, ZNF426, FUT2, and SERPINB2 exhibiting similar expression patterns in Fn-treated HIGK and HNSCC datasets. The GSDMD and NUP214 were found to be upregulated, and the genes ZNF426, FUT2, and SERPINB2 were downregulated. Among the five genes, the ZNF426 demonstrated a significant association with the survival of HNSCC patients. The low expression of ZNF426 presented a poor prognosis compared to the high expression. The study's results identified ZNF426 as a candidate gene involved in Fusobacterium nucleatum infection and HNSCC. Validating this result is necessary to gain insights into the role of the ZNF426 gene in developing HNSCC. Furthermore, probing the epigenetic factors targeting ZNF426 can be a potential therapeutic lead.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"59-71"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients. 结直肠癌患者血浆源性外泌体长链非编码rna (lncRNAs) CCAT1和XIST的临床相关性

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.51654.2061

Fatemeh Dana, Soleiman Mahjoub, Zahra Shokati Eshkiki, Abolfazl Namazi, Seidamir Pasha Tabaeian, Abolfazl Akbari

{"title":"Clinical relevance of plasma-derived exosomal long non-coding RNAs (lncRNAs) CCAT1 and XIST in colorectal cancer patients.","authors":"Fatemeh Dana, Soleiman Mahjoub, Zahra Shokati Eshkiki, Abolfazl Namazi, Seidamir Pasha Tabaeian, Abolfazl Akbari","doi":"10.22099/mbrc.2025.51654.2061","DOIUrl":"https://doi.org/10.22099/mbrc.2025.51654.2061","url":null,"abstract":"The expression level of exosomal long non-coding RNAs (lncRNAs) can be relevant for clinical diagnostic approaches. The object of our study was to evaluate the differential expression of lncRNAs colon cancer associated transcript 1 (CCAT1) and X-inactive specific transcript (XIST) in plasma exosomes of colorectal cancer (CRC) patients and investigate their potential as clinical biomarkers. In a case-control study, 62 CRC patients and 62 healthy persons were studied. Plasma exosomes were isolated by a centrifugation approach and were characterized by microscopy and western blotting. After RNA extraction and cDNA synthesis, using real-time PCR technique, the relative expression of lncRNAs was evaluated. The expression levels of lncRNA CCAT1, but not XIST, were meaningfully increased in the plasma-derived exosomes of CRC patients compared to non-cancer individuals (p= 0.001, 0.083 respectively). Further analyses revealed that the expression levels of exosomal lncRNA CCAT1 were associated with the lymphovascular invasion and tumor differentiation (p<0.05). ROC curve analysis documented a diagnostic power for lncRNA CCAT1 in CRC with a sensitivity of 79% and a specificity of 80% with an optimal cutoff point 6.5, with an area under curve (AUC)=86% and p<0.0001. Also, lncRNA XIST revealed a sensitivity of 62% and a specificity of 61% with a cutoff point 2.4, with an AUC=65%. Our findings indicated the potential of plasma-derived exosomal lncRNA CCAT1 as a non-invasive clinical indicator for the diagnosis of CRC patients.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"157-166"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational analysis of Ayurvedic herbs to explore their potential role as anti-cervical cancer agents. 阿育吠陀草药的计算分析探讨其作为抗宫颈癌药物的潜在作用。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.51173.2038

Suhani Dange, Neha Aggarwal, Rivi Verma, Yashika Sinha, Sonakshi Dadhiya, Gagan Mittal, Ruchi Sachdeva

{"title":"Computational analysis of Ayurvedic herbs to explore their potential role as anti-cervical cancer agents.","authors":"Suhani Dange, Neha Aggarwal, Rivi Verma, Yashika Sinha, Sonakshi Dadhiya, Gagan Mittal, Ruchi Sachdeva","doi":"10.22099/mbrc.2024.51173.2038","DOIUrl":"https://doi.org/10.22099/mbrc.2024.51173.2038","url":null,"abstract":"Cervical cancer is one of the common types of cancer in women. Treatment regimens include use of chemotherapy but it leads to certain side effects thereby creating a need for safer therapeutic options. Ayurveda has a great potential to provide better treatment strategies. In this study, computational approaches have been employed to investigate the molecular mechanism of anti-cervical cancer Ayurvedic herbs. Initially, Ayurvedic plants possessing anti-cervical cancer activities were obtained from literature. Bioactive compounds present in such plants were evaluated for drug-likeliness, biological functions and associations with cancer-related pathways. This resulted in the most promising drug-like bioactive compounds which were found to target cancer pathways like microRNA and proteoglycans, Human papillomavirus infection. Anti-cervical cancer activity possessing herbs derived from the study include Camellia sinensis, Equisetum arvense, Rosmarinus officinalis. Major bioactive compounds extracted from the enlisted herbs that contribute in promoting anti-cervical cancer effects include allicin, apigenin, and mataresinol. Overall, our study has provided insights into the scientific mechanism behind anti-cervical cancer activities of the indigenous herbs of Ayurveda. In addition, this study has also highlighted key bioactive compounds which have a potential in targeting cancer related pathways and thus can further be utilized to devise better therapeutics to cure cervical cancer.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 2","pages":"129-141"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on diabetes and oral cancer: Molecular links and implications. 糖尿病与口腔癌的分子联系及其意义

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.51225.2042

Jayaseelan Vijayashree Priyadharsini, Anitha Pandi

引用次数: 0

Gene co-expression network analysis reveals relationship between leukocyte fraction and genomic instability in dedifferentiated liposarcoma. 基因共表达网络分析揭示去分化脂肪肉瘤中白细胞比例与基因组不稳定性的关系。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.51329.2050

Mohammad Darzi, Mahdieh Shokrollahi-Barough, Elahe Nazeri, Keivan Majidzadeh-A, Rezvan Esmaeili

{"title":"Gene co-expression network analysis reveals relationship between leukocyte fraction and genomic instability in dedifferentiated liposarcoma.","authors":"Mohammad Darzi, Mahdieh Shokrollahi-Barough, Elahe Nazeri, Keivan Majidzadeh-A, Rezvan Esmaeili","doi":"10.22099/mbrc.2025.51329.2050","DOIUrl":"https://doi.org/10.22099/mbrc.2025.51329.2050","url":null,"abstract":"Dedifferentiated Liposarcoma (DDLPS) is one of the common subtypes of liposarcoma that is considered a highly malignant category. This study aims to investigate DDLPS through a system biology approach. The gene expression profiles and clinical traits of the DDLPS were acquired from The Cancer Genome Atlas (TCGA). The identification of co-expressed modules was conducted using the weighted gene co-expression network analysis. The immune cell-related gene function was studied by a web-based tool, TIMER, and, the survival analysis was performed at both the module and single-gene levels through Cox Regression analysis. Gene enrichment analysis was also conducted using the DAVID tool. One of the nine co-expressed DDLPS modules was significantly correlated with leukocyte fraction, hyper/hypo methylation, tumor purity, and chromosome instability (CIN). Based on the biological processes used to classify genes, the hub genes in a particular module play important roles in DNA repair, microtubule organizing clusters, mitotic checkpoint dysregulation, and cell proliferation signaling pathways. After screening the genes based on intra-module connectivity, module membership, and gene significance RAD54L was selected as one of the important hub genes. RAD54L showed poor prognosis to the overall survival (OS) analysis (HR=1.6, 95% CI=1.1-2.4, p=0.02). No co-expressed modules had relationship with OS. Through DDLPS traits, CIN and hyper/hypo methylation had significant negative relationship with OS. Our achievement confirmed the inverse association between tumor purity for DDLPS gene profiles and leukocyte fraction and negative leukocyte fraction (LF) gene significance in some genes was justified according to the sub-population analyses of immune cells in TIMER.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 3","pages":"203-218"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comment on: IL13 gene polymorphisms among Sudanese patients with bronchial asthma: a case-control study. 评论苏丹支气管哮喘患者的 IL13 基因多态性：一项病例对照研究。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.52030.2082

Zahra Zendehboodi

引用次数: 0

The importance of TP53 status in cancer therapy: The example of chronic lymphocytic leukemia. TP53在癌症治疗中的重要性：以慢性淋巴细胞白血病为例。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.51477.2054

Regina Mirgayazova, Raniya Khadiullina, Elvina Gilyazova, Damir Davletshin, Irina Ganeeva, Ekaterina Zmievskaya, Vitaly Chasov, Aygul Valiullina, Emil Bulatov

{"title":"The importance of TP53 status in cancer therapy: The example of chronic lymphocytic leukemia.","authors":"Regina Mirgayazova, Raniya Khadiullina, Elvina Gilyazova, Damir Davletshin, Irina Ganeeva, Ekaterina Zmievskaya, Vitaly Chasov, Aygul Valiullina, Emil Bulatov","doi":"10.22099/mbrc.2025.51477.2054","DOIUrl":"https://doi.org/10.22099/mbrc.2025.51477.2054","url":null,"abstract":"The TP53 gene encodes the tumor suppressor protein p53, which plays a critical role in genomic stability and cell cycle regulation. TP53 mutations are prevalent in approximately half of all human malignancies and are associated with poor clinical outcomes, including increased genomic instability, chemoresistance, and reduced survival rates. However, the prognostic and predictive value of TP53 status remains inconsistent across cancer types. Chronic lymphocytic leukemia (CLL) stands out as a disease where TP53 alterations have a well-established clinical significance, influencing treatment decisions and patient prognosis. In CLL, TP53 mutations and 17p deletions are strongly correlated with advanced disease stages, resistance to chemo-immunotherapy, and poor overall survival. The European Research Initiative for CLL (ERIC) has recognized TP53 status as a crucial prognostic biomarker, advocating for its routine assessment in clinical practice. Given the limitations of traditional therapies in TP53-mutated CLL, novel targeted therapies, including BCL2 and BTK inhibitors, as well as CAR-T cell therapy, are being explored to improve patient outcomes. This review provides an in-depth analysis of the evolving role of TP53 status in CLL, with a particular focus on emerging therapeutic strategies, including CAR-T cell therapy, and their potential to overcome TP53-driven treatment resistance.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 3","pages":"179-198"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Breast cancer susceptibility is associated with Cyclin D1 single nucleotide polymorphisms in Iran: A case-control study. 伊朗乳腺癌易感性与Cyclin D1单核苷酸多态性相关：一项病例对照研究

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2025.51763.2065

Sadaf Soleimani, Soheila Talesh Sasani, Zivar Salehi, Fereshteh Fakour

引用次数: 0

IL13 gene polymorphisms among Sudanese patients with bronchial asthma: a case-control study. 苏丹支气管哮喘患者IL13基因多态性：一项病例对照研究

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.50865.2017

Menas A Abdalla, Hamdan Z Hamdan

引用次数: 0

Evaluation of several strategies for controlling canker plant disease caused by Pseudomonas syringae. 对控制由丁香假单胞菌引起的植物腐烂病的几种策略进行评估。

IF 1.5

Molecular Biology Research Communications Pub Date : 2025-01-01 DOI: 10.22099/mbrc.2024.51122.2034

Reyhaneh Ravanbakhshian-HabibAbadi, Mandana Behbahani, Hassan Mohabatkar

{"title":"Evaluation of several strategies for controlling canker plant disease caused by Pseudomonas syringae.","authors":"Reyhaneh Ravanbakhshian-HabibAbadi, Mandana Behbahani, Hassan Mohabatkar","doi":"10.22099/mbrc.2024.51122.2034","DOIUrl":"10.22099/mbrc.2024.51122.2034","url":null,"abstract":"Pseudomonas syringae is a gram-negative bacterium that causes a diversity of diseases in numerous plants. Strategies to inhibit P. syringae growth include protective procedures; however, controlling the disease is complicated due to its rapid spread. Several antimicrobial agents can prevent this disease, such as chemical compounds, biological agents, secondary metabolites, nanoparticles, bacteriophages, and antimicrobial peptides (AMPs). The most effective way to control the disease is through chemical control. Using copper compounds and antibiotics is a conventional practice to decrease canker disease symptoms. However, due to environmental pollution caused by chemicals and bactericides and the resistance of different pathovars of P. syringae, other methods for bacterial pathogens control are needed. Biological control, using antagonistic bacteria has shown promising results against P. syringae under in vitro conditions. New studies focus on using secondary metabolites from plants to control plant diseases. Studies have shown that essential oils when preserved from degradation and evaporation by nanoparticles like mesoporous silica, can increase their antibacterial activities. Using nanoparticles, especially silver, is a suitable strategy for controlling P. syringae. However, high concentrations of silver nanoparticles are toxic. Bacteriophages and AMPs are recommended as alternatives to control bacterial infections in agriculture, including P. syringae. Combined treatments of phages and secondary metabolites have shown higher efficacy, potentially overcoming resistance. However, bacteriophages and AMPs are expensive and limited. In the end, using secondary metabolites and nanoparticles at low concentrations presents economic benefits and antibacterial activities without phytotoxic properties.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"14 1","pages":"1-14"},"PeriodicalIF":1.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11624607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0