Molecular Biology Research Communications最新文献

A strategic approach to genetic model selection in association studies: A practical guide. 关联研究中遗传模式选择的策略方法：实用指南。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2026.54476.2226

Mostafa Saadat

引用次数: 0

Artificial intelligence and genomic data privacy: Balancing innovation with security. 人工智能和基因组数据隐私：平衡创新与安全。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.54335.2217

Leila Kohan

引用次数: 0

Molecular dynamics simulation of thermal activation of human TRPV1. 人TRPV1热激活的分子动力学模拟。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.53301.2171

Juan David Bermudes-Contreras, Luis Manuel Arratia-Cortés, Maria Esther Ramírez-Moreno, Beatriz Zamora-López, Cesar López-Camarillo, Laurence A Marchat, Absalom Zamorano-Carrillo

{"title":"Molecular dynamics simulation of thermal activation of human TRPV1.","authors":"Juan David Bermudes-Contreras, Luis Manuel Arratia-Cortés, Maria Esther Ramírez-Moreno, Beatriz Zamora-López, Cesar López-Camarillo, Laurence A Marchat, Absalom Zamorano-Carrillo","doi":"10.22099/mbrc.2025.53301.2171","DOIUrl":"10.22099/mbrc.2025.53301.2171","url":null,"abstract":"TRPV1 (Transient Receptor Potential Vanilloid 1) is a non-selective ion channel that responds to various thermal, chemical, mechanical, and ligand stimuli. It is expressed in various tissues, mainly in nociceptive neurons, adipocytes, and other cell types. This channel is involved in pain and temperature transition processes, although it has recently been implicated in adipocyte browning processes. That is why the study of this receptor has increased in recent years to understand the process of activation and inactivation by various stimuli. In this work, we focus on modeling a complete channel of human TRPV1 (hTRPV1), the structural changes, and especially the behavior of the pore when this protein is subjected to high temperatures (400 K). We report that when molecular dynamics simulate hTRPV1 at 400 K, it suffers an increase in the diameter of the two gates reported elsewhere in the pore, suggesting the opening of the channel. In this work, we describe the structural changes in the entire protein, concomitant to those in the pore, favoring this process, which might be associated with its biological activity.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 1","pages":"39-48"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145677711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic potential of 4-OH-coumarin in neuroblastoma: cellular, molecular, and epigenetic insights. 4- oh -香豆素在神经母细胞瘤中的治疗潜力：细胞、分子和表观遗传学的见解。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.54532.2228

Çağrı Öner, Halise Elif Kulakac, Damla Kolcuoğlu, Ertuğrul Çolak

{"title":"Therapeutic potential of 4-OH-coumarin in neuroblastoma: cellular, molecular, and epigenetic insights.","authors":"Çağrı Öner, Halise Elif Kulakac, Damla Kolcuoğlu, Ertuğrul Çolak","doi":"10.22099/mbrc.2025.54532.2228","DOIUrl":"https://doi.org/10.22099/mbrc.2025.54532.2228","url":null,"abstract":"The effect of 4-OH-Coumarin, a warfarin derivate, on the cellular characteristics and metastasis of SH-SY5Y neuroblastoma cells and HUVEC cells was aimed to determine. After IC50 concentrations were detected wound healing and hematoxylin-eosin assays were performed on both cell lines. Ki-67, hTERT, PI3K, AKT, mTOR, HIF-1α, PINK1, Parkin, Cyt C, p53 gene expressions and piR-651, piR-823, miR-126 expressions were determined by RT-PCR. The proliferation, wound closure and survival decreased after 4-OH-Coumarin treatment (p<0.001). Ki-67, hTERT, PI3K, mTOR, HIF-1α, PINK1, Parkin, Cyt C and piR-823 expressions were decreased, while AKT, p53, piR-651, and miR-126 increased on SH-SY5Y (p<0.001). AKT (p<0.05), Parkin, and piR-651 expressions increased on only HUVEC cells (p<0.001). We believe that the study of various molecules that are secondary metabolites such as 4-OH-Coumarin may provide valuable data to observe the effects and mechanisms of new therapeutics that have the potential to be used for cancer treatment.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 2","pages":"103-116"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13148452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CPUK02 sensitizes U87 glioblastoma cell lines to TMZ treatment via autophagy flux inhibition. CPUK02通过抑制自噬通量使U87胶质母细胞瘤细胞系对TMZ敏感。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.52011.2079

Hooman Rezaie, Sanaz Dastghaib, Morvarid Siri, Pooneh Mokarram, Mina Hemmati

{"title":"CPUK02 sensitizes U87 glioblastoma cell lines to TMZ treatment via autophagy flux inhibition.","authors":"Hooman Rezaie, Sanaz Dastghaib, Morvarid Siri, Pooneh Mokarram, Mina Hemmati","doi":"10.22099/mbrc.2025.52011.2079","DOIUrl":"10.22099/mbrc.2025.52011.2079","url":null,"abstract":"Adjuvant chemotherapy with TMZ (Temozolomide) does not improve the survival of patients suffering from GBM (Glioblastoma). Given the importance of autophagy and UPR (Unfolding Protein Response) in chemotherapy resistance, as well as the role of Beclin-1, LC3IIβ, and P62 in the regulation of autophagy, we evaluated the effect of TMZ along with CPUK02 on U87 cells as a model of Glioblastoma cancer in this study. To achieve this goal, we treated the U87 cells with different doses of TMZ (50, 100, 200, 400, and 800 μM) and CPUK02 (1, 0.5, 0.25, 0.125, 0.06, 0.03, 0.01, and 0.007 μM); then, cell viability was assessed by MTT assay. The gene expression of Beclin1, P62, LC3IIβ, and XBP-1s was analyzed using quantitative real-time polymerase chain reaction. The comparison of the control group with the groups treated with the TMZ drug showed that, in 48 and 72 hours, doses of TMZ more than IC50 (100 μM) (p<0.001) significantly led to cell death. CPUK02 doses more than 0.125 (p<0.0001) significantly led to cell death. TMZ and CPUK02 combination therapy (100 and 0.03 μM, respectively) increased the expression of Beclin-1, LC3IIβ, and P62 and activated the IRE-1 arm of UPR by increasing the expression of XBP-1s. TMZ and CPUK02 treatment inhibits the autophagic flux (p62, LC3IIβ). Increased XBP-1s expression might contribute to the enhanced TMZ sensitivity. This combination therapy is promising for TMZ-resistant cancers, but it needs further investigation.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 1","pages":"11-20"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145678261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review on the potential of modulating the IRE arm of the UPR in U87 and U251 glioblastoma cells for improved therapeutic efficacy. 系统回顾了在U87和U251胶质母细胞瘤细胞中调节UPR的IRE臂以提高治疗效果的潜力。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.54152.2205

Mehdi Ghavamizadeh, Roozbeh Kiani, Fatemeh Shams, Parmis Taghizadeh, Ali Honari-Jahromi, Seyed Mohammad Hossein Afzali-Joibari, Mozhdeh Zamani, Sanaz Dastghaib, Pooneh Mokarram

{"title":"A systematic review on the potential of modulating the IRE arm of the UPR in U87 and U251 glioblastoma cells for improved therapeutic efficacy.","authors":"Mehdi Ghavamizadeh, Roozbeh Kiani, Fatemeh Shams, Parmis Taghizadeh, Ali Honari-Jahromi, Seyed Mohammad Hossein Afzali-Joibari, Mozhdeh Zamani, Sanaz Dastghaib, Pooneh Mokarram","doi":"10.22099/mbrc.2025.54152.2205","DOIUrl":"https://doi.org/10.22099/mbrc.2025.54152.2205","url":null,"abstract":"Glioblastoma multiforme (GBM) remains the most aggressive primary brain tumor with poor prognosis and limited response to current therapies. Recent studies suggest that the unfolded protein response (UPR), particularly the inositol-requiring enzyme 1 (IRE1) signaling arm, plays a pivotal role in GBM pathophysiology by mediating cellular adaptation to endoplasmic reticulum stress. This systematic review evaluates the role of IRE1 in GBM cell lines (U87, U251) and investigates whether its activation or inhibition affects migration, proliferation, apoptosis, and cell death. A comprehensive search was conducted on PubMed/ Medline, Scopus, Web of Science, and Embase using various keywords up to October 14, 2025, following the PRISMA guidelines. The search aimed to identify original English-language studies that specifically examined and analyzed the IRE1 arm of the UPR pathway in glioblastoma cells. Out of 466 records, 26 studies met the inclusion criteria. Twenty studies explored IRE1 activation, while six investigated its inhibition. IRE1 activation yielded dual effects-promoting apoptosis via JNK or XBP1 pathways in some contexts, while supporting tumor survival and angiogenesis through XBP1-mediated transcription and RIDD suppression in others. Dual role of IRE1 could sensitize GBM cells to chemotherapy agents, reduced migration and proliferation, and induced apoptosis. IRE1 acts as a context-dependent regulator in GBM, showing both pro-survival and pro-death roles. Most studies report that IRE1 activation promotes glioblastoma cell death, while fewer address its inhibition. Thus, both activation and inhibition may offer therapeutic potential depending on cellular context and downstream signaling.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 2","pages":"85-101"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13148453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and Morphological evidence support the delimitation of a new genus, Hafezia gen. nov. in Arabideae (Brassicaceae). 分子和形态学证据支持拟南芥科Hafezia gen. 11新属的划分。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.54085.2198

Ahmad Reza Khosravi, Atena Eslami-Farouji

{"title":"Molecular and Morphological evidence support the delimitation of a new genus, Hafezia gen. nov. in Arabideae (Brassicaceae).","authors":"Ahmad Reza Khosravi, Atena Eslami-Farouji","doi":"10.22099/mbrc.2025.54085.2198","DOIUrl":"https://doi.org/10.22099/mbrc.2025.54085.2198","url":null,"abstract":"Recent molecular phylogenetic analyses within the tribe Arabideae (Brassicaceae) have uncovered unresolved lineages that challenge current generic boundaries. In this study, we incorporate molecular data with morphological evidence to resolve a long-standing taxonomic ambiguity, resulting in the description of a new monophyletic genus, Hafezia gen. nov., together with two new combinations, H. aucheri (Boiss.) A.R. Khosravi & A. Eslami-Farouji and H. parvula (Dufour ex DC.) A.R. Khosravi & A. Eslami-Farouji. Integrative taxonomy and phylogenetic insights, such as indels and substitution nucleotide variants, confirm the distinct assignment of these taxa, previously misassigned to Arabis L. Morphological reassessment, including traits such as simple, long setaceous hairs, notched petals, winged stamens, not compressed, not subtorulose or torulose fruits, supports their separation. The new genus naturally grows in the Mediterranean and west of the Irano-Turanian floristic regions. Our findings highlight the significance of integration of taxonomic investigations with the molecular investigations in clarifying evolutionary relationships and provide a revised key for accurate identification within Arabideae.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 2","pages":"117-130"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13148450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to the Letter: Observations and points of interest regarding bioinformatics predictions of miRNA targeting syncytin genes in endometriosis and miscarriage. 对信函的回应：关于miRNA靶向合胞素基因在子宫内膜异位症和流产中的生物信息学预测的观察和兴趣点。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.55184.2256

Somayeh Shatizadeh-Malekshahi

引用次数: 0

Observations and points of interest regarding bioinformatics predictions of miRNA targeting syncytin genes in endometriosis and miscarriage. 子宫内膜异位症和流产中靶向合胞素基因的miRNA生物信息学预测的观察和兴趣点。

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.55165.2254

Piruz Shadbash, Marzieh Bahari-Babadi

引用次数: 0

The possibility of prognostic and functional values of the 8q24 and 20q13 chromosomal bands in colorectal cancer. 8q24和20q13染色体带在结直肠癌中预后和功能价值的可能性

IF 1

Molecular Biology Research Communications Pub Date : 2026-01-01 DOI: 10.22099/mbrc.2025.54114.2202

Seyed Ahmadreza Siadat

{"title":"The possibility of prognostic and functional values of the 8q24 and 20q13 chromosomal bands in colorectal cancer.","authors":"Seyed Ahmadreza Siadat","doi":"10.22099/mbrc.2025.54114.2202","DOIUrl":"10.22099/mbrc.2025.54114.2202","url":null,"abstract":"Colorectal cancer (CRC) remains a major global health concern, especially given its increasing incidence among younger individuals. While genome-wide association studies (GWAS) have identified numerous CRC-associated polymorphisms, their spatial distribution and functional implications are not fully understood. This study examined the locations of 1,346 CRC-linked polymorphisms across chromosomal bands. The results revealed significant nonrandom clustering across thirteen chromosomal bands: 1q41, 6p21, 8q24, 9q34, 10p14, 10q25, 11q12, 12p13, 15q13, 18q21, 19q13, 20p12, and 20q13. Functional enrichment analysis of genes within these bands revealed several Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Reciprocal chromosomal enrichment confirmed that many of these terms and pathways were not randomly localized within the same bands, highlighting their potential biological significance. Survival analysis using TCGA data identified three KEGG pathways and 33 GO terms mapped to nine of the thirteen bands that were significantly associated with poor prognosis. Notably, the 8q24 and 20q13 regions were enriched for differentially expressed genes and survival-associated terms yet showed no significant enrichment for genes with high somatic mutation rates. These results imply that 8q24 and 20q13 act as regulatory hotspots rather than mutation-driven regions. Overall, this integrative approach identified functionally and clinically relevant genomic regions that may contribute to inherited CRC risk and progression, providing valuable targets for the development of diagnostic and prognostic biomarkers.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"15 1","pages":"3-10"},"PeriodicalIF":1.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12673626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145677752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0