Molecular Biology Research Communications最新文献_第10页

Computational insights into fluconazole resistance by the suspected mutations in lanosterol 14α-demethylase (Erg11p) of Candida albicans. 白色念珠菌羊毛甾醇14α-去甲基化酶(Erg11p)可疑突变对氟康唑耐药性的计算见解

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-12-01 DOI: 10.22099/mbrc.2020.36298.1476

Sagunthala Murugesan Udaya Prakash, Yasin Nazeer, Sivaraman Jayanthi, Mohammad Anaul Kabir

{"title":"Computational insights into fluconazole resistance by the suspected mutations in lanosterol 14α-demethylase (Erg11p) of Candida albicans.","authors":"Sagunthala Murugesan Udaya Prakash, Yasin Nazeer, Sivaraman Jayanthi, Mohammad Anaul Kabir","doi":"10.22099/mbrc.2020.36298.1476","DOIUrl":"https://doi.org/10.22099/mbrc.2020.36298.1476","url":null,"abstract":"Mutations in the ergosterol biosynthesis gene 11 (ERG11) of Candida albicans have been frequently reported in fluconazole-resistant clinical isolates. Exploring the mutations and their effect could provide new insights into the underlying mechanism of fluconazole resistance. Erg11p_Threonine285Alanine (Erg11p_THR285ALA), Erg11p_Leucine321Phenylalanine (Erg11p_LEU321PHE) and Erg11p_Serine457Proline (Erg11p_SER457PRO) are three fluconazole-resistant suspected mutations reported in clinical isolates of C. albicans. Therefore, our study aims to investigate the role of these suspected mutations in fluconazole resistance using in-silico methods. Molecular dynamics simulation (MDS) analysis of apo-protein for 25ns (nanosecond) showed that suspected mutant proteins underwent slight conformational changes in the tertiary structure. Molecular docking with fluconazole followed by binding free energy analysis showed reduced non-bonded interactions with loss of heme interaction and the least binding affinity for Erg11p_SER457PRO mutation. MDS of suspected mutant proteins-fluconazole complexes for 50ns revealed that Erg11p_SER457PRO and Erg11p_LEU321PHE have clear differences in the interaction pattern and loss or reduced heme interaction compared to wild type Erg11p-fluconazole complex. MDS and binding free energy analysis of Erg11p_SER457PRO-fluconazole complex showed the least binding similar to verified mutation Erg11p_TYR447HIS-fluconazole complex. Taken together, our study concludes that suspected mutation Erg11p_THR285ALA may not have any role whereas Erg11p_LEU321PHE could have a moderate role. However, Erg11p_SER457PRO mutation has a strong possibility to play an active role in fluconazole resistance of C. albicans.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 4","pages":"155-167"},"PeriodicalIF":1.6,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38734045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Differential expression of inflammatory responsive genes between chronic periodontitis and periodontally affected bronchiectasis patients. 慢性牙周炎与牙周影响支气管扩张患者炎症反应基因的差异表达。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-12-01 DOI: 10.22099/mbrc.2020.37397.1508

Abhaya Gupta, Neetu Singh, Anil Kumar, Umesh Pratap Verma, Ajay Kumar Verma, Hari Shyam, Nand Lal, Surya Kant, Ankur Kumari

{"title":"Differential expression of inflammatory responsive genes between chronic periodontitis and periodontally affected bronchiectasis patients.","authors":"Abhaya Gupta, Neetu Singh, Anil Kumar, Umesh Pratap Verma, Ajay Kumar Verma, Hari Shyam, Nand Lal, Surya Kant, Ankur Kumari","doi":"10.22099/mbrc.2020.37397.1508","DOIUrl":"https://doi.org/10.22099/mbrc.2020.37397.1508","url":null,"abstract":"The study aimed to investigate differential expression of targeted inflammatory-immune responsive genes [LTA, LTB, TNFSF4, TNFSF11/RANKL, TNFSF13, TNFSF13B, TNFRSF11B/ Osteoprotegerin; OPG and GFPT1/GFA ] in gingival tissues of bronchiectasis patients having chronic periodontitis in North central Indian population. Gingival tissues were collected from 30 systemically healthy chronic periodontitis patients (CP), 30 bronchiectasis patients with chronic periodontitis (B+CP), 3 systemically healthy with healthy gingiva (healthy control; HC) and 3 bronchiectasis with healthy gingiva (bronchiectasis control; BC). Statistical analysis revealed 7 genes to be significantly upregulated on comparing CP with B+CP i.e LTA (P<0.0001) in B+CP while LTB (P<0.0001), TNFSF4 (P=0.0003), TNFSF11 (P<0.0001), TNFSF13 (P=0.0003), TNFSF13B (P<0.0001) and TNFRSF11B (P=0.0004) in CP group. LTA (Lymphotoxin A) gene could be a potential genetic marker in bronchiectasis patients with chronic periodontitis.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 4","pages":"169-172"},"PeriodicalIF":1.6,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38734046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Differential genes expression analysis of invasive aspergillosis: a bioinformatics study based on mRNA/microRNA. 侵袭性曲霉病差异基因表达分析:基于mRNA/microRNA的生物信息学研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-12-01 DOI: 10.22099/mbrc.2020.37432.1509

Maryam Hosseinipour, Shirin Shahbazi, Shahla Roudbar-Mohammadi, Maryam Khorasani, Majid Marjani

{"title":"Differential genes expression analysis of invasive aspergillosis: a bioinformatics study based on mRNA/microRNA.","authors":"Maryam Hosseinipour, Shirin Shahbazi, Shahla Roudbar-Mohammadi, Maryam Khorasani, Majid Marjani","doi":"10.22099/mbrc.2020.37432.1509","DOIUrl":"https://doi.org/10.22099/mbrc.2020.37432.1509","url":null,"abstract":"Invasive aspergillosis is a severe opportunistic infection with high mortality in immunocompromised patients. Recently, the roles of microRNAs have been taken into consideration in the immune system and inflammatory responses. Using bioinformatics approaches, we aimed to study the microRNAs related to invasive aspergillosis to understand the molecular pathways involved in the disease pathogenesis. Data were extracted from the gene expression omnibus (GEO) database. We proposed 3 differentially expressed genes; S100B, TDRD9 and TMTC1 related to pathogenesis of invasive aspergillosis. Using miRWalk 2.0 predictive tool, microRNAs that targeted the selected genes were identified. The roles of microRNAs were investigated by microRNA target prediction and molecular pathways analysis. The significance of combined expression changes in selected genes was analyzed by ROC curves study. Thirty-three microRNAs were identified as the common regulator of S100B, TDRD9 and TMTC1 genes. Several of them were previously reported in the pathogenesis of fungal infections including miR-132. Predicted microRNAs were involved in innate immune response as well as toll-like receptor signaling. Most of the microRNAs were also linked to platelet activation. The ROC chart in the combination mode of S100B/TMTC1, showed the sensitivity of 95.65 percent and the specificity of 69.23 percent. New approaches are needed for rapid and accurate detection of invasive aspergillosis. Given the pivotal signaling pathways involved, predicted microRNAs can be considered as the potential candidates of the disease diagnosis. Further investigation of the microRNAs expression changes and related pathways would lead to identifying the effective biomarkers for IA detection.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 4","pages":"173-180"},"PeriodicalIF":1.6,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38734047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Association of two single nucleotide polymorphisms rs10407022 and rs3741664 with the risk of primary ovarian insufficiency in a sample of Iraqi women. 伊拉克妇女样本中两个单核苷酸多态性rs10407022和rs3741664与原发性卵巢功能不全风险的关联

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-12-01 DOI: 10.22099/mbrc.2020.36371.1477

Tamadher Abbas Rafaa, Ahmed AbdulJabbar Suleiman, Mustafa Falah Dawood, Ali Mohammed Al-Rawi

引用次数: 0

Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay. 用PCR法测定设拉子省消费的未经巴氏消毒的乳制品中布鲁氏菌的流行情况。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.37381.1506

Fargol Abdali, Saeid Hosseinzadeh, Enayat Berizi, Maryam Pourmontaseri

{"title":"Prevalence of Brucella species in unpasteurized dairy products consumed in Shiraz province using PCR assay.","authors":"Fargol Abdali, Saeid Hosseinzadeh, Enayat Berizi, Maryam Pourmontaseri","doi":"10.22099/mbrc.2020.37381.1506","DOIUrl":"https://doi.org/10.22099/mbrc.2020.37381.1506","url":null,"abstract":"The consumption of milk and unpasteurized dairy products contaminated with Brucella bacteria is one of the most important ways of brucellosis transmission to humans. The principal goal of this study was to determine the prevalence of Brucella abortus (B. abortus) and Brucella melitens (B. melitens) in unpasteurized dairy products consumed in Shiraz province. In this study conducted in 2016, 238 unpasteurized dairy products including 48 raw milk, 48 yogurt, 46 cheeses, 48 dough and 48 ice cream samples, were purchased from the retail market in Shiraz province and were examined by a specific PCR assay. This study showed positive 5/04% out of 238 unpasteurized dairy products including 9 out of 48 (18/75%) raw milk samples and 3 out of 48 (6.25%) yogurt samples). Contamination was not detected in samples of dough, cheese and traditional ice cream. The results also showed that among 12 positive samples, 6 samples were contaminated with B. abortus (including 4 milk samples and 2 yogurt samples), 2 samples were contaminated with B. melitensis (including 2 Milk samples) and 4 samples were contaminated simultaneously with B. abortus and B. melitensis (including 3 milk samples and 1 yogurt sample). The present study suggests the unpasteurized dairy products as the major sources of brucellosis in Shiraz province, South of Iran; thus, to prevent brucellosis in human, the consumption of pasteurized milk and dairy products is highly recommended.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"117-121"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38704942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Sequence variants of CYP345a1 and CYP6a14 gene regions in Tribolium castaneum (Coleoptera: Tenebrionidae) adults treated with the novel characterızed Bolanthus turcicus (Caryophyllaceae) extract. 新提取物characterızed处理后的黄褐虫(鞘翅目:拟甲科)成虫CYP345a1和CYP6a14基因区序列变异

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.35861.1472

Fahriye Sümer Ercan, Serap Yalçın Azarkan, Nuri Ercan, Murat Koc

{"title":"Sequence variants of CYP345a1 and CYP6a14 gene regions in Tribolium castaneum (Coleoptera: Tenebrionidae) adults treated with the novel characterızed Bolanthus turcicus (Caryophyllaceae) extract.","authors":"Fahriye Sümer Ercan, Serap Yalçın Azarkan, Nuri Ercan, Murat Koc","doi":"10.22099/mbrc.2020.35861.1472","DOIUrl":"https://doi.org/10.22099/mbrc.2020.35861.1472","url":null,"abstract":"In this study, various doses of plant extracts that obtained from Bolanthus turcicus was applied to an important storage pest Tribolium castaneum adults. Bolanthus turcicus is an endemic species and spreads on the Hasan Mountain above Karkın town (Turkey, Aksaray province). The plant species was collected from June to July with the field study to be carried out in this region. Obtained extract of plant was analyzed by gas chromatography mass spectrometry (GC-MS) method. The doses were defined during the study and the concentrations that kill 50% and 99% of the population were determined after applications. After 24 h, DNA was isolated from live and dead individuals that obtained from LC50 and LC99 concentration applications and analyzed for Cytochrome P450-mediated detoxification resistance genes, CYP345A1 and CYP6A14 gene regions, by polymerase chain reaction (PCR). CYP genes in insects are known to be rapidly regulated when exposed to insecticides. In the study, in order to screen for 206 bp and 353 bp fragments of CYP345A1 and CYP6A14 genes in T. castaneum adults were amplified using specific primers, respectively. DNA direct sequencing was performed on each template using the forward primer. When compared to the control, it is believed that mutation differences in live and dead individuals according to the sequencing results obtained from survival and dead adults, may allow these genes to play a protective role against the toxic effect of B. turcicus extract.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"105-110"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727768/pdf/mbrc-9-105.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38707493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Deciphering the functional role of hypothetical proteins from Chloroflexus aurantiacs J-10-f1 using bioinformatics approach. 用生物信息学方法解读金莲J-10-f1假想蛋白的功能作用。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.36894.1495

Chander Jyoti Thakur, Sandeep Saini, Aayushi Notra, Bhavanshu Chauhan, Sarthak Arya, Rishabh Gupta, Jyotsna Thakur, Varinder Kumar

{"title":"Deciphering the functional role of hypothetical proteins from Chloroflexus aurantiacs J-10-f1 using bioinformatics approach.","authors":"Chander Jyoti Thakur, Sandeep Saini, Aayushi Notra, Bhavanshu Chauhan, Sarthak Arya, Rishabh Gupta, Jyotsna Thakur, Varinder Kumar","doi":"10.22099/mbrc.2020.36894.1495","DOIUrl":"https://doi.org/10.22099/mbrc.2020.36894.1495","url":null,"abstract":"Chloroflexus aurantiacus J-10-f1 is an anoxygenic, photosynthetic, facultative autotrophic gram negative bacterium found from hot spring at a temperature range of 50-60°C. It can sustain itself in dark only if oxygen is available thereby exhibiting a dark orange color, however display a dark green color when grown in sunlight. Genome of the organism contains total of 3853 proteins out of which 785 (~20%) proteins are uncharacterised or hypothetical proteins (HPs). Therefore in this work we have characterized the 785 hypothetical proteins of Chloroflexus aurantiacus J-10-f1 using bioinformatics tools and databases. HPs annotated by more than five domain prediction tools were filtered and named high confidence-hypothetical proteins (HC-HPs). These HC-HPs were further annotated by calculating their physiochemical properties, homologous, subcellular locations, signal peptides and transmembrane regions. We found most of the HC-HPs were involved in photosynthesis, carbohydrate metabolism, biofuel production and cellulose synthesis processes. Furthermore, few of these HC-HPs could provide resistance to bacteria at high temperature due to their thermophilic nature. Hence these HC-HPs have the potential to be used in industrial as well as in biomedical needs. To conclude, the bioinformatics approach used in this study provides an insight to better understand the nature and role of Chloroflexus aurantiacus J-10-f1 hypothetical proteins.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"129-139"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38704944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Evaluation of the risk of lung cancer associated with NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism in male current cigarette smokers from the Eastern India. 东印度男性吸烟者NAD(P)H:醌氧化还原酶1 (NQO1) C609T多态性与肺癌风险的评估

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.36467.1481

Santanu Banerjee

{"title":"Evaluation of the risk of lung cancer associated with NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism in male current cigarette smokers from the Eastern India.","authors":"Santanu Banerjee","doi":"10.22099/mbrc.2020.36467.1481","DOIUrl":"https://doi.org/10.22099/mbrc.2020.36467.1481","url":null,"abstract":"NAD(P)H: quinone oxidoreductase 1 (NQO1) is an endogenous cellular defence mechanism against several carcinogenic quinones derived from cigarette smoke. NQO1 C609T polymorphism is a strong determinant of NQO1 structure and function. The people with mutant allele for this polymorphism has significantly reduced NQO1 activity. In this study, we tried to evaluate the risk of lung cancer associated with this polymorphism in male current smokers of the Eastern India. Using PCR-RFLP method, we compared the NQO1 C609T genotype distribution in male current smokers with (n=150) and without (n=200) lung cancer. We observed significant variation of genotypic distribution between these two groups. The allele frequency of the variant C609T allele were 40.3% and 32.7% in smokers with and without lung cancer, respectively. From the genotypic comparison between the two smoker groups, it was found that a higher risk (OR=1.64, 95% CI: 1.05-2.55, P<0.05) of lung cancer was associated with NQO1 C609T polymorphism.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"111-115"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727764/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38704941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel chimeric recombinant protein PDHB-P80 of Mycoplasma agalactiae as a potential diagnostic tool. 一种新的无乳支原体嵌合重组蛋白PDHB-P80作为潜在的诊断工具。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.37684.1513

Malihe Akbarzadeh-Niaki, Abdollah Derakhshandeh, Nasrin Kazemipour, Vida Eraghi, Farhid Hemmatzadeh

{"title":"A novel chimeric recombinant protein PDHB-P80 of Mycoplasma agalactiae as a potential diagnostic tool.","authors":"Malihe Akbarzadeh-Niaki, Abdollah Derakhshandeh, Nasrin Kazemipour, Vida Eraghi, Farhid Hemmatzadeh","doi":"10.22099/mbrc.2020.37684.1513","DOIUrl":"https://doi.org/10.22099/mbrc.2020.37684.1513","url":null,"abstract":"The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC main workbench 5.5 software. Several linkers and three dimensional structure of PDHB-P80 were compared to the native PDHB and analyzed to select a proper one for expression. The fusion gene sequence was optimized and synthesized in pMAT cloning vector. The synthetic pMAT-pdhb-p80 was digested using Bam HI and Sal I restriction enzymes and ligated into pMAL-p5X expression vector. The pMAL-pdhb-p80 construct was transfected into E.coli BL21 strain cells and expressed protein were purified using amylose resin. and the purified protein was analyzed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. In silico analysis demonstrated that fusion proteins using IgG4 middle hinge (CPSCP) with TM-score of 0.99 showed the higher similarity between three dimensional structure of PDHB before and after fusion with high antigenic region of P80. Successful cloning verified by PCR colony, double digestion and sequence analysis. Besides, SDS-PAGE analysis and Western blotting indicated and confirmed the expression of intact recombinant chimeric protein MBP-PDHB-P80 along with some truncated forms of the recombinant protein. it could be concluded that the fusion construct has a potential for serodiagnostic assay in future studies.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"123-128"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38704943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Using two retrotransposon-based marker systems (SRAP and REMAP) for genetic diversity analysis of Moroccan Argan tree. 利用两个基于反转录转座子的标记系统(SRAP和REMAP)对摩洛哥坚果树进行遗传多样性分析。

IF 1.6

Molecular Biology Research Communications Pub Date : 2020-09-01 DOI: 10.22099/mbrc.2020.36390.1478

Ouafae Pakhrou, Leila Medraoui, Bouchra Belkadi, Farid Rachidi, Hasnaa Errahmani, Mohammed Alami, Abdelkarim Filali-Maltouf

{"title":"Using two retrotransposon-based marker systems (SRAP and REMAP) for genetic diversity analysis of Moroccan Argan tree.","authors":"Ouafae Pakhrou, Leila Medraoui, Bouchra Belkadi, Farid Rachidi, Hasnaa Errahmani, Mohammed Alami, Abdelkarim Filali-Maltouf","doi":"10.22099/mbrc.2020.36390.1478","DOIUrl":"https://doi.org/10.22099/mbrc.2020.36390.1478","url":null,"abstract":"The Argania is an endemic genetic resource in Morocco holding an important ecological and socio-economical benefit. However, overgrazing and overharvesting lead to a serious downturn in the number of trees. To characterize genetic diversity within and among 24 populations, represented by 240 argan trees, four combinations of SRAP primers and eight combinations of REMAP primers were used. A total of 338 REMAP and 146 SRAP markers were amplified with a polymorphism of 100%. The average polymorphism information content value was 0.20 and 0.17 for SRAP and REMAP markers, respectively. The analysis of molecular variance showed that 26% of the genetic variation was partitioned among populations. The coefficient of gene differentiation was 0.2875 and gene flow was 1.2391. The average parameter diversity was: observed number of alleles (Na)=0.729, effective number of alleles (Ne)=1.131, Shannon's information index (I)=1.143; Nei's gene diversity (H)=0.093 and Percentage of Polymorphic Loci=35.68. The STRUCTURE and principal coordinate analysis revealed that the Argania spinosa L. populations were aggregated into 2 genetic groups. To detect outlier, baysecan software was used and 21 were detected (7 under selection, 14 under balancing selection) presenting posterior probability higher than 0.79. The current results can be explored in the design of management programs and to comprehend the adaptation mechanism of Argan tree.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"9 3","pages":"93-103"},"PeriodicalIF":1.6,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7727765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38707492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6