Molecular Biology Research Communications最新文献_第9页

Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer. 脂联素rs2241766和rs266729多态性与甲状腺乳头状癌风险的相关性

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43012.1714

Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari

{"title":"Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer.","authors":"Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari","doi":"10.22099/mbrc.2022.43012.1714","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43012.1714","url":null,"abstract":"About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"113-118"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2. SARS-CoV-2刺突、包膜、膜蛋白和ssRNA功能单元的胁迫适应特征

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44594.1777

Aniket Sarkar, Anindya Sundar Panja

{"title":"Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2.","authors":"Aniket Sarkar, Anindya Sundar Panja","doi":"10.22099/mbrc.2022.44594.1777","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44594.1777","url":null,"abstract":"Pandemic coronavirus causes respiratory, enteric and sometimes neurological diseases. Proteome data of individual coronavirus strains were already reported. Here we investigated of SARS-CoV-2 ssRNA and protein of spike, envelope and membrane to determine stress adaptation profile. Thermodynamic properties, Physicochemical behaviour and, amino acid composition along with their RMSD value was analysed. Thermodynamic index of SARS-CoV2 spike, envelope and membrane ssRNA is unstable in higher temperature. Presence of higher proportion of polar with positive and negative charged amino acid residues into spike (S), envelope (E) and membrane (M) protein indicate the lower stress adaptability pattern. Our study represented several unstable pockets into S, E and M proteins of SARS-CoV-2 against different abiotic stresses, specifically higher in spike protein. Contact with heat through solvent may denature the architectural network of SARS-CoV-2 spike, envelope and membrane ssRNA and structural protein. The stress instability index of SARS-CoV-2 and the interactome profile of its transmembrane proteins may help to reveal novel factors for inhibiting SARS-CoV-2 growth.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"155-166"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. 阿富汗乙肝表面抗原携带者丁型肝炎的系统发育分析和流行情况。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44692.1780

Abbas Ali Husseini, Mehran Rostamzadeh

引用次数: 0

Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. miR-142在甲状腺乳头状癌组织中的上调:基于计算机和体外分析的报告。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43947.1757

Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi

{"title":"Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis.","authors":"Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi","doi":"10.22099/mbrc.2022.43947.1757","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43947.1757","url":null,"abstract":"Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls. 研究toll样受体9 rs5743836、rs352140和rs187084基因多态性及其mRNA水平与非酒精性脂肪肝患者不同肝纤维化阶段的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43852.1753

Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali

{"title":"Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls.","authors":"Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali","doi":"10.22099/mbrc.2022.43852.1753","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43852.1753","url":null,"abstract":"Recent studies have shown that the level of hepatocyte-derived mitochondrial DNA is elevated in plasma samples obtained from mice and NASH patients, and it has the ability to toll-like receptor 9 (TLR9) activation resulting in steatosis, hepatocyte injury, and fibrosis. In this study, we explored the association between TLR9 rs5743836, rs352140, and rs187084 polymorphism and its plasma mRNA level in non-alcoholic fatty liver (NAFL) patients with different liver fibrosis scores compared to healthy controls. Seventy Iranian patients diagnosed with NAFL, based on fibroscan testing results, were divided into F0-F1 (N=33), F2-F3 (N=19), and F4 (N=18) hepatic fibrosis groups and compared to 22 healthy controls. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the mRNA expression level of TLR9 was determined using Real-Time PCR analysis. Results showed no significant association between allelic and genotypic distribution frequency of TLR9 rs5743836, rs352140, and rs187084 polymorphisms in NAFL patients with hepatic fibrosis compared to healthy controls (P>0.05). However, the mRNA level of TLR9 was significantly elevated in correlation with hepatic fibrosis progression in NAFL patients compared to healthy controls (P<0.05). As a preliminary study, our data showed a correlative overexpression of TLR9 mRNA with hepatic fibrosis progression in NAFL patients without the effectiveness of TLR9 gene polymorphisms.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"119-126"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in CFI and ARMS genes and exudative age-related macular degeneration: Correspondence. CFI和ARMS基因多态性与渗出性年龄相关性黄斑变性:对应关系。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44737.1782

Rujittika Mungmunpuntipantip, Viroj Wiwanitkit

引用次数: 0

Novel RNA extraction method from human tears. 从人泪液中提取RNA的新方法。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.45266.1801

Mahintaj Dara, Azam Habibi, Negar Azarpira, Mehdi Dianatpour, Mahmood Nejabat, Amir Khosravi, Nader Tanideh

引用次数: 0

Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway. 甲状腺乳头状癌中差异表达mirna及靶基因预测的研究与确认，重点关注自噬信号通路。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43844.1751

Mojtaba Zehtabi, Zahra Akbarpour, Sepehr Valizadeh, Yousef Roosta, Amir Mahdi Khamaneh, Mortaza Raeisi

{"title":"Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway.","authors":"Mojtaba Zehtabi, Zahra Akbarpour, Sepehr Valizadeh, Yousef Roosta, Amir Mahdi Khamaneh, Mortaza Raeisi","doi":"10.22099/mbrc.2022.43844.1751","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43844.1751","url":null,"abstract":"Papillary thyroid carcinoma (PTC) is the most common endocrine cancer. However, the role of biomechanics in the development and progression of PTC is obscure. The microarray dataset GSE104005 was examined to identify important microRNAs (miRNAs or miRs) and their probable roles in the carcinogenesis of PTC. The gene expression omnibus (GEO) database was used to obtain the data. R was used to access the differentially expressed miRNAs (DEMs) and genes (DEGs). The multiMiR software was used to predict DEM targets. To validate the top DEMs and DEGs, thirty tissue samples were obtained from PTC patients who had their thyroids removed and compared with 30 normal samples. The total RNA content of the tumor and corresponding non-tumoral adjacent samples were purified and converted to cDNA. Expression levels of top dysregulated miRNAs and their target and predicted DEG were evaluated using the RT-qPCR method. miR-182 and miR-183 were top upregulated miRs and miR-30d was the most downregulated miR among DEMs. Furthermore, FOXO1 which was shown to be targeted by aforementioned miRNAs, was the most downregulated genes among other DEGs. 10 hub nodes were detected by PPI construction. PTEN was the hub node with highest score. The in vitro gene expression analysis was also showed the same expression pattern in tissues. Significant increase in miR-182-5p and miR-183-5p expressions, as well as a significant decrease in FOXO1 and miR-30d-5p expressions, suggest that PTC cells may tend to preserve their autophagy capability.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"173-181"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10707067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa. 铜绿假单胞菌临床样品生物膜形成的表型和分子研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.41708.1673

Leila Dolatshah, Mohammad Tabatabaei

{"title":"A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa.","authors":"Leila Dolatshah, Mohammad Tabatabaei","doi":"10.22099/mbrc.2021.41708.1673","DOIUrl":"https://doi.org/10.22099/mbrc.2021.41708.1673","url":null,"abstract":"Pseudomonas aeruginosa is identified as a versatile opportunistic microorganism with metabolic diversity contributing to a wide range of health burdens, especially in immunocompromised patients. This bacterium is the cause of 10 to 20% of nosocomial infections. In this study, we evaluated the phenotypic characterizations of biofilm formation in P. aeruginosa clinical isolates using micro-titer plate assay. Indeed, we estimated the prevalence of QS (rhlI, rhlR, rhlAB, lasB, lasI, lasR, aprA) and virulence genes (pslA and cupA) by PCR. The results showed that among 69% of the isolates forming biofilm, 9% were strong biofilm producers, whereas 13% and 47% of isolates produced moderate and low amounts of biofilm, respectively. All isolates possessed cupA and seven QS genes (rhlI, rhlR, rhlAB, lasB, lasI, lasR, aprA), while 92% of the isolates possessed the pslA gene. Identification of these genes and their association with biofilm formation can be advantageous in adopting therapeutic methods.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"157-163"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis and comparison of physiochemical properties, mutations and glycosylation patterns between RNA polymerase and membrane protein of SARS-CoV and SARS-CoV-2. SARS-CoV和SARS-CoV-2 RNA聚合酶和膜蛋白的理化性质、突变和糖基化模式分析与比较

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.42187.1692

Mandana Behbahani, Parisa Rabiei, Hassan Mohabatkar

{"title":"Analysis and comparison of physiochemical properties, mutations and glycosylation patterns between RNA polymerase and membrane protein of SARS-CoV and SARS-CoV-2.","authors":"Mandana Behbahani, Parisa Rabiei, Hassan Mohabatkar","doi":"10.22099/mbrc.2021.42187.1692","DOIUrl":"10.22099/mbrc.2021.42187.1692","url":null,"abstract":"SARS-CoV-2 is a member of β-genus of the coronavirus subfamily, alongside the virus that causes SARS (Severe Acute Respiratory Syndrome). As implied by their names, SARS-CoV-2 and SARS-CoV genome sequences have close kinship (about 79% genomic sequence similarity). In the current research, sequence-based physiochemical properties of RNA polymerase and membrane glycoprotein of SARS-CoV-2 and SARS-CoV were compared. In addition, impacts of substitution mutations on stability and glycosylation patterns of these proteins were studied. In comparison of physiochemical features of membrane and RNA polymerase proteins, only instability index of membrane protein was difference between SARS-CoV and SARS-CoV-2. Mutation analysis showed increase in stability of RNA polymerase and decrease in stability of membrane protein in SARS-CoV-2. Glycosylation pattern analysis showed glycosylation enhancement in both membrane and RNA polymerase proteins of SARS-CoV-2 in comparison to SARS-CoV. In conclusion, more glycosylation and stability of SARS-CoV-2 RNA polymerase could be one of the reasons of high pathogenicity property and host immune system evasion of SARS-CoV-2.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"171-178"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1