Molecular Biology Research Communications最新文献_第8页

Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats 海藻糖对衰老大鼠肾脏NFE2L2、过氧化氢酶和超氧化物歧化酶的影响

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42014.1688

Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi

{"title":"Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats","authors":"Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi","doi":"10.22099/mbrc.2022.42014.1688","DOIUrl":"https://doi.org/10.22099/mbrc.2022.42014.1688","url":null,"abstract":"Aging is associated with an increase in oxidative stress, which damages organs such as the kidney. Trehalose has abundant beneficial activities including antioxidative effects. This study aimed to investigate the effects of trehalose on several antioxidant parameters of the aged kidney. Wistar rats were divided into three groups: young (4 months), aged (24 months), and aged-trehalose. The third group was treated with 2% trehalose for one month. The expression of target genes and enzyme activities in the kidney of the animals were evaluated by quantitative polymerase chain reaction (qPCR) and enzyme colorimetric procedures, respectively. Protein levels of NFE2L2 showed a 50% reduction in aged rats compared to young rats (P<0.001), which was restored by trehalose intervention. In addition, the activity and mRNA levels of catalase (CAT) increased in aged rats while treatment with trehalose reversed this trend. On the other hand, superoxide dismutase (SOD) activity was reduced in the kidneys of aged rats but was not affected by trehalose intervention .It is concluded that trehalose supplementation alleviates the antioxidant system impairments in the kidneys of aged rats. However, further investigations are needed to thoroughly describe the antioxidative impacts of trehalose on the kidneys during aging.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 1","pages":"29 - 36"},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42857164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toward a better understanding of phylogenetic relationships within Conringieae (Brassicaceae) 更好地了解芸苔科锥属植物的系统发育关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/MBRC.2022.42767.1709

A. Khosravi, Atena Eslami-Farouji, Atiqullah Sultani-Ahmadzai, S. Mohsenzadeh

引用次数: 5

Identification of Bordetella bronchiseptica in the throat and nose of dogs and cats by PCR. 用聚合酶链反应(PCR)鉴定犬、猫咽喉和鼻腔中的支气管脓毒杆菌。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43873.1755

Mohammad Tabatabaei, Hamid Reza Rohani

引用次数: 0

Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate. 推测产细菌素分离物杜兰肠球菌NT21的基因组分析。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44088.1760

Nashwa Tarek, Ahmed O El-Gendy, Ahmed S Khairalla, Medhat Abdel-Fattah, Eman Tawfik, Ahmed F Azmy

{"title":"Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate.","authors":"Nashwa Tarek, Ahmed O El-Gendy, Ahmed S Khairalla, Medhat Abdel-Fattah, Eman Tawfik, Ahmed F Azmy","doi":"10.22099/mbrc.2022.44088.1760","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44088.1760","url":null,"abstract":"Enterococcus species are a long-standing and non-pathogenic commensal bacterium, representing an important part of the normal. Enterococcus durans is a rarely isolated species from animals and humans, and it was a tiny constituent of human oral cavity and animal intestinal flora, as well as animal-derived foods, particularly dairy products. This study evaluated the security of our strain E. durans NT21 by using whole-genome sequencing (WGS), physicochemical features, and antimicrobial activity. The complete genomic of our strain Enterococcus durans NT21was sequenced and analyzed by using several bioinformatics tools to identify bacteriocin genes, virulence genes, antibiotic resistance genes, Crispr-Cas and pathogenicity islands. The results showed that our strain NT21 lacks the presence of virulence genes, pathogenicity islands, plasmids and has only two antibiotic resistance genes. On the other hand, it produces three bacteriocin-like inhibitory substances (Enterolysin A, P and L50a). It has six gene-encoded Crisper-Cas and one cluster Crispr-Cas gene. According to our findings, E. durans NT21 is a possible probiotic strain that is safe for both human and animal use.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"143-153"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer. 脂联素rs2241766和rs266729多态性与甲状腺乳头状癌风险的相关性

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43012.1714

Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari

{"title":"Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer.","authors":"Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari","doi":"10.22099/mbrc.2022.43012.1714","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43012.1714","url":null,"abstract":"About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"113-118"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2. SARS-CoV-2刺突、包膜、膜蛋白和ssRNA功能单元的胁迫适应特征

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44594.1777

Aniket Sarkar, Anindya Sundar Panja

{"title":"Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2.","authors":"Aniket Sarkar, Anindya Sundar Panja","doi":"10.22099/mbrc.2022.44594.1777","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44594.1777","url":null,"abstract":"Pandemic coronavirus causes respiratory, enteric and sometimes neurological diseases. Proteome data of individual coronavirus strains were already reported. Here we investigated of SARS-CoV-2 ssRNA and protein of spike, envelope and membrane to determine stress adaptation profile. Thermodynamic properties, Physicochemical behaviour and, amino acid composition along with their RMSD value was analysed. Thermodynamic index of SARS-CoV2 spike, envelope and membrane ssRNA is unstable in higher temperature. Presence of higher proportion of polar with positive and negative charged amino acid residues into spike (S), envelope (E) and membrane (M) protein indicate the lower stress adaptability pattern. Our study represented several unstable pockets into S, E and M proteins of SARS-CoV-2 against different abiotic stresses, specifically higher in spike protein. Contact with heat through solvent may denature the architectural network of SARS-CoV-2 spike, envelope and membrane ssRNA and structural protein. The stress instability index of SARS-CoV-2 and the interactome profile of its transmembrane proteins may help to reveal novel factors for inhibiting SARS-CoV-2 growth.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"155-166"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. 阿富汗乙肝表面抗原携带者丁型肝炎的系统发育分析和流行情况。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44692.1780

Abbas Ali Husseini, Mehran Rostamzadeh

引用次数: 0

Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. miR-142在甲状腺乳头状癌组织中的上调:基于计算机和体外分析的报告。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43947.1757

Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi

{"title":"Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis.","authors":"Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi","doi":"10.22099/mbrc.2022.43947.1757","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43947.1757","url":null,"abstract":"Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls. 研究toll样受体9 rs5743836、rs352140和rs187084基因多态性及其mRNA水平与非酒精性脂肪肝患者不同肝纤维化阶段的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43852.1753

Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali

{"title":"Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls.","authors":"Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali","doi":"10.22099/mbrc.2022.43852.1753","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43852.1753","url":null,"abstract":"Recent studies have shown that the level of hepatocyte-derived mitochondrial DNA is elevated in plasma samples obtained from mice and NASH patients, and it has the ability to toll-like receptor 9 (TLR9) activation resulting in steatosis, hepatocyte injury, and fibrosis. In this study, we explored the association between TLR9 rs5743836, rs352140, and rs187084 polymorphism and its plasma mRNA level in non-alcoholic fatty liver (NAFL) patients with different liver fibrosis scores compared to healthy controls. Seventy Iranian patients diagnosed with NAFL, based on fibroscan testing results, were divided into F0-F1 (N=33), F2-F3 (N=19), and F4 (N=18) hepatic fibrosis groups and compared to 22 healthy controls. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the mRNA expression level of TLR9 was determined using Real-Time PCR analysis. Results showed no significant association between allelic and genotypic distribution frequency of TLR9 rs5743836, rs352140, and rs187084 polymorphisms in NAFL patients with hepatic fibrosis compared to healthy controls (P>0.05). However, the mRNA level of TLR9 was significantly elevated in correlation with hepatic fibrosis progression in NAFL patients compared to healthy controls (P<0.05). As a preliminary study, our data showed a correlative overexpression of TLR9 mRNA with hepatic fibrosis progression in NAFL patients without the effectiveness of TLR9 gene polymorphisms.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"119-126"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in CFI and ARMS genes and exudative age-related macular degeneration: Correspondence. CFI和ARMS基因多态性与渗出性年龄相关性黄斑变性:对应关系。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44737.1782

Rujittika Mungmunpuntipantip, Viroj Wiwanitkit

引用次数: 0