海藻糖对衰老大鼠肾脏NFE2L2、过氧化氢酶和超氧化物歧化酶的影响

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI:10.22099/mbrc.2022.42014.1688

Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi

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引用次数: 1

摘要

衰老与氧化应激的增加有关，氧化应激会损害肾脏等器官。海藻糖具有丰富的有益活性，包括抗氧化作用。本研究旨在探讨海藻糖对衰老肾脏若干抗氧化参数的影响。Wistar大鼠分为三组：幼龄（4个月）、老龄（24个月）和老龄海藻糖。第三组用2%海藻糖治疗一个月。分别通过定量聚合酶链式反应（qPCR）和酶比色法评估动物肾脏中靶基因的表达和酶活性。与年轻大鼠相比，老年大鼠的NFE2L2蛋白水平降低了50%（P<0.001），海藻糖干预使其恢复。此外，衰老大鼠的过氧化氢酶（CAT）活性和mRNA水平增加，而海藻糖治疗逆转了这一趋势。另一方面，衰老大鼠肾脏超氧化物歧化酶（SOD）活性降低，但不受海藻糖干预的影响。然而，还需要进一步的研究来彻底描述海藻糖在衰老过程中对肾脏的抗氧化作用。

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Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats

Aging is associated with an increase in oxidative stress, which damages organs such as the kidney. Trehalose has abundant beneficial activities including antioxidative effects. This study aimed to investigate the effects of trehalose on several antioxidant parameters of the aged kidney. Wistar rats were divided into three groups: young (4 months), aged (24 months), and aged-trehalose. The third group was treated with 2% trehalose for one month. The expression of target genes and enzyme activities in the kidney of the animals were evaluated by quantitative polymerase chain reaction (qPCR) and enzyme colorimetric procedures, respectively. Protein levels of NFE2L2 showed a 50% reduction in aged rats compared to young rats (P<0.001), which was restored by trehalose intervention. In addition, the activity and mRNA levels of catalase (CAT) increased in aged rats while treatment with trehalose reversed this trend. On the other hand, superoxide dismutase (SOD) activity was reduced in the kidneys of aged rats but was not affected by trehalose intervention .It is concluded that trehalose supplementation alleviates the antioxidant system impairments in the kidneys of aged rats. However, further investigations are needed to thoroughly describe the antioxidative impacts of trehalose on the kidneys during aging.

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来源期刊

Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.