Molecular Biology Research Communications最新文献_第7页

Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis. miR-142在甲状腺乳头状癌组织中的上调:基于计算机和体外分析的报告。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43947.1757

Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi

{"title":"Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis.","authors":"Sepehr Valizadeh, Mojtaba Zehtabi, Neda Feiziordaklou, Zahra Akbarpour, Amir Mahdi Khamaneh, Mortaza Raeisi","doi":"10.22099/mbrc.2022.43947.1757","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43947.1757","url":null,"abstract":"Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"133-141"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls. 研究toll样受体9 rs5743836、rs352140和rs187084基因多态性及其mRNA水平与非酒精性脂肪肝患者不同肝纤维化阶段的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43852.1753

Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali

{"title":"Investigating the association of toll-like receptor 9 rs5743836, rs352140, and rs187084 gene polymorphisms and their mRNA levels with different hepatic fibrosis stages of non-alcoholic fatty liver patients compared to healthy controls.","authors":"Azadeh Rezaie, Meysam Nasiri, Behzad Hatami, Kaveh Baghaie, Hamid Asadzadeh-Aghdaei, Mohammad Reza Zali","doi":"10.22099/mbrc.2022.43852.1753","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43852.1753","url":null,"abstract":"Recent studies have shown that the level of hepatocyte-derived mitochondrial DNA is elevated in plasma samples obtained from mice and NASH patients, and it has the ability to toll-like receptor 9 (TLR9) activation resulting in steatosis, hepatocyte injury, and fibrosis. In this study, we explored the association between TLR9 rs5743836, rs352140, and rs187084 polymorphism and its plasma mRNA level in non-alcoholic fatty liver (NAFL) patients with different liver fibrosis scores compared to healthy controls. Seventy Iranian patients diagnosed with NAFL, based on fibroscan testing results, were divided into F0-F1 (N=33), F2-F3 (N=19), and F4 (N=18) hepatic fibrosis groups and compared to 22 healthy controls. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the mRNA expression level of TLR9 was determined using Real-Time PCR analysis. Results showed no significant association between allelic and genotypic distribution frequency of TLR9 rs5743836, rs352140, and rs187084 polymorphisms in NAFL patients with hepatic fibrosis compared to healthy controls (P>0.05). However, the mRNA level of TLR9 was significantly elevated in correlation with hepatic fibrosis progression in NAFL patients compared to healthy controls (P<0.05). As a preliminary study, our data showed a correlative overexpression of TLR9 mRNA with hepatic fibrosis progression in NAFL patients without the effectiveness of TLR9 gene polymorphisms.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"119-126"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymorphisms in CFI and ARMS genes and exudative age-related macular degeneration: Correspondence. CFI和ARMS基因多态性与渗出性年龄相关性黄斑变性:对应关系。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44737.1782

Rujittika Mungmunpuntipantip, Viroj Wiwanitkit

引用次数: 0

Novel RNA extraction method from human tears. 从人泪液中提取RNA的新方法。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.45266.1801

Mahintaj Dara, Azam Habibi, Negar Azarpira, Mehdi Dianatpour, Mahmood Nejabat, Amir Khosravi, Nader Tanideh

引用次数: 0

Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway. 甲状腺乳头状癌中差异表达mirna及靶基因预测的研究与确认，重点关注自噬信号通路。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43844.1751

Mojtaba Zehtabi, Zahra Akbarpour, Sepehr Valizadeh, Yousef Roosta, Amir Mahdi Khamaneh, Mortaza Raeisi

{"title":"Investigation and confirmation of differentially expressed miRNAs, as well as target gene prediction in papillary thyroid cancer, with a special emphasis on the autophagy signaling pathway.","authors":"Mojtaba Zehtabi, Zahra Akbarpour, Sepehr Valizadeh, Yousef Roosta, Amir Mahdi Khamaneh, Mortaza Raeisi","doi":"10.22099/mbrc.2022.43844.1751","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43844.1751","url":null,"abstract":"Papillary thyroid carcinoma (PTC) is the most common endocrine cancer. However, the role of biomechanics in the development and progression of PTC is obscure. The microarray dataset GSE104005 was examined to identify important microRNAs (miRNAs or miRs) and their probable roles in the carcinogenesis of PTC. The gene expression omnibus (GEO) database was used to obtain the data. R was used to access the differentially expressed miRNAs (DEMs) and genes (DEGs). The multiMiR software was used to predict DEM targets. To validate the top DEMs and DEGs, thirty tissue samples were obtained from PTC patients who had their thyroids removed and compared with 30 normal samples. The total RNA content of the tumor and corresponding non-tumoral adjacent samples were purified and converted to cDNA. Expression levels of top dysregulated miRNAs and their target and predicted DEG were evaluated using the RT-qPCR method. miR-182 and miR-183 were top upregulated miRs and miR-30d was the most downregulated miR among DEMs. Furthermore, FOXO1 which was shown to be targeted by aforementioned miRNAs, was the most downregulated genes among other DEGs. 10 hub nodes were detected by PPI construction. PTEN was the hub node with highest score. The in vitro gene expression analysis was also showed the same expression pattern in tissues. Significant increase in miR-182-5p and miR-183-5p expressions, as well as a significant decrease in FOXO1 and miR-30d-5p expressions, suggest that PTC cells may tend to preserve their autophagy capability.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"173-181"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10707067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa. 铜绿假单胞菌临床样品生物膜形成的表型和分子研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.41708.1673

Leila Dolatshah, Mohammad Tabatabaei

{"title":"A phenotypic and molecular investigation of biofilm formation in clinical samples of Pseudomonas aeruginosa.","authors":"Leila Dolatshah, Mohammad Tabatabaei","doi":"10.22099/mbrc.2021.41708.1673","DOIUrl":"https://doi.org/10.22099/mbrc.2021.41708.1673","url":null,"abstract":"Pseudomonas aeruginosa is identified as a versatile opportunistic microorganism with metabolic diversity contributing to a wide range of health burdens, especially in immunocompromised patients. This bacterium is the cause of 10 to 20% of nosocomial infections. In this study, we evaluated the phenotypic characterizations of biofilm formation in P. aeruginosa clinical isolates using micro-titer plate assay. Indeed, we estimated the prevalence of QS (rhlI, rhlR, rhlAB, lasB, lasI, lasR, aprA) and virulence genes (pslA and cupA) by PCR. The results showed that among 69% of the isolates forming biofilm, 9% were strong biofilm producers, whereas 13% and 47% of isolates produced moderate and low amounts of biofilm, respectively. All isolates possessed cupA and seven QS genes (rhlI, rhlR, rhlAB, lasB, lasI, lasR, aprA), while 92% of the isolates possessed the pslA gene. Identification of these genes and their association with biofilm formation can be advantageous in adopting therapeutic methods.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"157-163"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798273/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis and comparison of physiochemical properties, mutations and glycosylation patterns between RNA polymerase and membrane protein of SARS-CoV and SARS-CoV-2. SARS-CoV和SARS-CoV-2 RNA聚合酶和膜蛋白的理化性质、突变和糖基化模式分析与比较

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.42187.1692

Mandana Behbahani, Parisa Rabiei, Hassan Mohabatkar

{"title":"Analysis and comparison of physiochemical properties, mutations and glycosylation patterns between RNA polymerase and membrane protein of SARS-CoV and SARS-CoV-2.","authors":"Mandana Behbahani, Parisa Rabiei, Hassan Mohabatkar","doi":"10.22099/mbrc.2021.42187.1692","DOIUrl":"10.22099/mbrc.2021.42187.1692","url":null,"abstract":"SARS-CoV-2 is a member of β-genus of the coronavirus subfamily, alongside the virus that causes SARS (Severe Acute Respiratory Syndrome). As implied by their names, SARS-CoV-2 and SARS-CoV genome sequences have close kinship (about 79% genomic sequence similarity). In the current research, sequence-based physiochemical properties of RNA polymerase and membrane glycoprotein of SARS-CoV-2 and SARS-CoV were compared. In addition, impacts of substitution mutations on stability and glycosylation patterns of these proteins were studied. In comparison of physiochemical features of membrane and RNA polymerase proteins, only instability index of membrane protein was difference between SARS-CoV and SARS-CoV-2. Mutation analysis showed increase in stability of RNA polymerase and decrease in stability of membrane protein in SARS-CoV-2. Glycosylation pattern analysis showed glycosylation enhancement in both membrane and RNA polymerase proteins of SARS-CoV-2 in comparison to SARS-CoV. In conclusion, more glycosylation and stability of SARS-CoV-2 RNA polymerase could be one of the reasons of high pathogenicity property and host immune system evasion of SARS-CoV-2.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"171-178"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer? 前列腺组织中MMP-9、TIMP-1、RECK和miR338-3p的表达:能否预测癌症?

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.40912.1646

Rodolfo Pacheco de Moraes, Ruan Pimenta, Fernando Noboru Cabral Mori, Gabriel Arantes Dos Santos, Nayara Izabel Viana, Vanessa Ribeiro Guimarães, Juliana Alves de Camargo, Katia Ramos Moreira Leite, Miguel Srougi, William Carlos Nahas, Sabrina T Reis

{"title":"Tissue expression of MMP-9, TIMP-1, RECK, and miR338-3p in prostate gland: can it predict cancer?","authors":"Rodolfo Pacheco de Moraes, Ruan Pimenta, Fernando Noboru Cabral Mori, Gabriel Arantes Dos Santos, Nayara Izabel Viana, Vanessa Ribeiro Guimarães, Juliana Alves de Camargo, Katia Ramos Moreira Leite, Miguel Srougi, William Carlos Nahas, Sabrina T Reis","doi":"10.22099/mbrc.2021.40912.1646","DOIUrl":"https://doi.org/10.22099/mbrc.2021.40912.1646","url":null,"abstract":"Prostate cancer is the most frequent malignancy affecting men worldwide. Due to the low sensitivity and specificity of the prostate-specific antigen test and the digital rectal exam as screening modalities, several alternatives are being studied. This study aimed to evaluate the application of MMP-9 and its regulators (TIMP-1, RECK, and miR-338-3p) as diagnostic and prognostic indicators of prostate cancer. A total of 134 randomly selected patients under investigation for prostate cancer submitted to a transrectal ultrasound-guided prostate biopsy were enrolled in the study; of these, 61 were positive for the disease (cases), and 73 were negative (control group). The tissue samples were further analyzed by gene and miR-338-3p expression analysis using qRT-PCR (one randomly selected fragment of each patient). Approximately 58% of the patients with prostate cancer presented MMP9 upregulation, while 73%, 65%, and 69% downregulated IMP-1, RECK, and miR-338-3p, respectively. MiR-338-3p was expressed at lower levels in patients with PSA concentrations exceeding 20 ng/mL (p=0.045) and abnormal DRE (p=0.006), while the RECK was more expressed in patients with abnormal DRE (p=0.01). We found that most patients with prostate cancer overexpressed MMP-9; on the other hand, most of them underexpressed TIMP-1, RECK, and miR-338-3p. MiR-338-3p presented as a possible predictor of poor prognosis. Further studies are warranted to evaluate these biomarkers as prognosis factors better.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"149-156"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation of p16 protein expression and its association with histopathologic parameters in breast cancer. 乳腺癌中p16蛋白表达及其与组织病理参数关系的研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.41691.1671

Siamak Naji-Haddadi, Daniel Elieh-Ali-Komi, Saeid Aghayan, Rahim Asghari, Javad Rasouli

{"title":"Investigation of p16 protein expression and its association with histopathologic parameters in breast cancer.","authors":"Siamak Naji-Haddadi, Daniel Elieh-Ali-Komi, Saeid Aghayan, Rahim Asghari, Javad Rasouli","doi":"10.22099/mbrc.2021.41691.1671","DOIUrl":"https://doi.org/10.22099/mbrc.2021.41691.1671","url":null,"abstract":"We investigated the association between p16 expression and histopathologic parameters including size, neural and vascular invasion, and lymph node involvement in breast cancer. 58 specimens from patients with different grades of breast cancer were included. Hematoxylin and eosin and immunohistochemistry staining for p16 was performed. 5 patients (8.6%) had grade I, 23 (39.7%) had grade II, and 30 (51.7%) had grade III breast cancer. Assessment of the tumor size showed that 5 (8.6%) tumors had a size of ≤2cm, 29 (50%) were between 2-5 cm and 24 (41.4%) had a size of ≥5cm. Moreover, 45 (77.6%) of the included patients had axillary lymph node involvement. Investigation of association between p16 positivity with pathological parameters in three groups with positivity to p16 (1-25%, 26-75%, >75%) showed that there was no association between p16 positivity and other parameters including histologic score (p=0.44), tumor size (p=0.77), neural invasion (p=0.79), perivascular invasion (p=0.98) and the number of involved LNs (p=0.49). From the group including eight patients with >75% p16 positivity, seven (87.5%) were found with neural invasion and two (25%) with perivascular invasion. P16 positivity was not associated with size, neural and vascular invasion, and LN involvement in breast cancer.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"165-170"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39750399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fission yeast Ase1^PRC1 is required for the G₂-microtubule damage response. 裂变酵母Ase1PRC1是g2微管损伤反应所必需的。

IF 1.6

Molecular Biology Research Communications Pub Date : 2021-12-01 DOI: 10.22099/mbrc.2021.41001.1650

Rose M Doss, Sindi Xhunga, Dorothy Klimczak, Molly Cameron, Jordan Verlare, Tom D Wolkow

{"title":"Fission yeast Ase1PRC1 is required for the G2-microtubule damage response.","authors":"Rose M Doss, Sindi Xhunga, Dorothy Klimczak, Molly Cameron, Jordan Verlare, Tom D Wolkow","doi":"10.22099/mbrc.2021.41001.1650","DOIUrl":"https://doi.org/10.22099/mbrc.2021.41001.1650","url":null,"abstract":"Schizosaccharomyces pombe delays entry into mitosis following G2 microtubule damage. This pathway is dependent on Rad26ATRIP, the regulatory subunit of the Rad26ATRIP/Rad3ATR DNA damage response (DDR) complex. However, this G2 microtubule damage response pathway acts independently of the G2 DNA damage checkpoint pathway. To identify other proteins in this G2 microtubule damage pathway, we previously screened a cDNA overexpression library for genes that rescued the sensitivity of rad26Δ cells to the microtubule poison thiabendazole. A partial cDNA fragment encoding only the C-terminal regulatory region of the microtubule bundling protein Ase1 PRC1 was isolated. This fragment lacks the Ase1PRC1 dimerization and microtubule binding domains and retains the conserved C-terminal unstructured regulatory region. Here, we report that ase1Δ cells fail to delay entry into mitosis following G2 microtubule damage. Microscopy revealed that Rad26ATRIP foci localized alongside Ase1PRC1 filaments, although we suggest that this is related to microtubule-dependent double strand break mobility that facilitates homologous recombination events. Indeed, we report that the DNA repair protein Rad52 co-localizes with Rad26ATRIP at these foci, and that localization of Rad26ATRIP to these foci depends on a Rad26ATRIP N-terminal region containing a checkpoint recruitment domain. To our knowledge, this is the first report implicating Ase1PRC1 in regulation of the G2/M transition.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"10 4","pages":"179-188"},"PeriodicalIF":1.6,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8798275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39573397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0