Molecular Biology Research Communications最新文献_第6页

Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells 替米沙坦和多烯紫杉醇联合对人前列腺癌和乳腺癌癌症细胞生存能力和转移基因表达的影响

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42638.1700

Marjan Khorsand, Z. Mostafavi-Pour, V. Razban, S. Khajeh, R. Zare

{"title":"Combinatorial effects of telmisartan and docetaxel on cell viability and metastatic gene expression in human prostate and breast cancer cells","authors":"Marjan Khorsand, Z. Mostafavi-Pour, V. Razban, S. Khajeh, R. Zare","doi":"10.22099/mbrc.2022.42638.1700","DOIUrl":"https://doi.org/10.22099/mbrc.2022.42638.1700","url":null,"abstract":"The epithelial-to-mesenchymal transition (EMT) is a unique process resulting in enhanced cell motility, invasiveness, and metastasis in cancer. The EMT is regulated by several transcription factors, including Snail and Slug, which exert crucial roles during cancer progression. We have studied the effects of Docetaxel as the first-line chemotherapy agent for prostate cancer, and Telmisartan as an anti-hypertensive drug on the expression level of Snail and Slug. In addition, the effects of Docetaxel, Telmisartan and their combination on cancer cell proliferation were investigated. The PC3, DU145, MDA-MB468, and HEK cell lines were used for this study. Quantitative RT-PCR analysis and MTT assay were used to study the expression of Snail and Slug level and cell proliferative assay, respectively. We found that a combination of Docetaxel + Telmisartan effectively inhibits the cell proliferation in cancerous cells in comparison with each drug alone (P<0.05). Furthermore, in these cell lines, Docetaxel, Telmisartan and their combination significantly diminished the expression level of Snail and Slug genes compared to control cells (P<0.001), however, in the HEK cell line, this effect was seen only in the combination group. Our data imply that Telmisartan and its combination with Docetaxel exert strong inhibitory effects on the expression level of Snail and Slug genes. Also, these drugs and their combination could inhibit cancer cell proliferation. In conclusion, the combination of Telmisartan and Docetaxel has the potential to suppress the metastasis of prostate and breast cancer cells.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 1","pages":"11 - 20"},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47751928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study 通过疫苗学方法设计一种新的非小细胞肺癌多表位肽疫苗:一项计算机研究

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42468.1697

A/Prof. Fatemeh Heidary MD, MPH, FAAO, FICO, M. Tourani, Fatemeh Hejazi-Amiri, S. H. Khatami, N. Jamali, Mortaza Taheri‐Anganeh

{"title":"Design of a new multi-epitope peptide vaccine for non-small cell Lung cancer via vaccinology methods: an in silico study","authors":"A/Prof. Fatemeh Heidary MD, MPH, FAAO, FICO, M. Tourani, Fatemeh Hejazi-Amiri, S. H. Khatami, N. Jamali, Mortaza Taheri‐Anganeh","doi":"10.22099/mbrc.2022.42468.1697","DOIUrl":"https://doi.org/10.22099/mbrc.2022.42468.1697","url":null,"abstract":"Lung cancer is the most common type of tumor worldwide. Non-small-cell lung carcinoma (NSCLC) is considered any epithelial cell-related lung cancer, which includes more than 85% of all lung cancer cases. NSCLC is less responsive to chemotherapy than SCLC. Therefore, the need for other treatments has become more pronounced and immunotherapy has gained increasing attention as a promising therapy in recent years. The current study aimed to design a multi-epitope peptide vaccine targeting main cancer/testis antigens of SP17, AKAP4, and PTTG1, which have a major function in tumor cell proliferation invasion. The protein vaccine was constructed using the rigorous immunoinformatics analysis and investigation of several immune system parameters, considering B cell epitopes and CD4 and CD8 induced epitopes as the most important cells to respond to cancer cells. Inverse translation and optimization of codons were performed to have the designed protein's cloning as well as expression potential in E.coli. Physicochemical, antigenic, and allergenic features were assessed to confirm the safety and immunogenicity of the vaccine. The secondary and tertiary structures were predicted. Finally, intrinsic disorder and 3D model refinement and validation were performed to eliminate structural problems. The designed construct had a stable structure that could be an antigen and stimulate the immune system and not be an allergen. The built model 3D structure was valid and stable. Further investigations are needed to approve the safety and immunogenic property of this new vaccine for NSCLC before it can be used in patients.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 1","pages":"55 - 66"},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43214704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Recombinase-free cloning (RFC) protocol for gene swapping 用于基因交换的无重组酶克隆（RFC）协议

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2021.41923.1685

Hai-Vy Vo-Nguyen, T. Nguyen, Quoc-Gia Mai, Thien-Thien Tran, T. L. Tran, Hieu Tran‐Van

{"title":"Recombinase-free cloning (RFC) protocol for gene swapping","authors":"Hai-Vy Vo-Nguyen, T. Nguyen, Quoc-Gia Mai, Thien-Thien Tran, T. L. Tran, Hieu Tran‐Van","doi":"10.22099/mbrc.2021.41923.1685","DOIUrl":"https://doi.org/10.22099/mbrc.2021.41923.1685","url":null,"abstract":"Recombinant DNA technology has been playing the key role for a long time since its first beginning. DNA ligases have certainly contributed to the development of cloning techniques, as well as molecular study up to now. Despite being a prime cloning tool, DNA ligases still face some shortcomings which lead to their limit of use. Our study provided an improved method that simplified the basic restriction enzyme-based cloning (REC) by eliminating the ligation role, named recombinase-free cloning (RFC). This improved technique was designed with only one PCR reaction, one digestion reaction, and one temperature profile, which takes advantage of endogenous recombinase in E. coli host to create the target recombinant vector inside the cell. All purification steps were eliminated for effectively material- and time-saving. Five different clones were generated by RFC. This method showed relatively low efficiency yet successful at a range of 100% in every conducted trial with fragment sizes from 0.5-1.0 kbp. The RFC method could be completed within a day (about 9 hours), without the need of ligase or recombinase or purification steps, which significantly saved DNA components, materials as well as the time required. In conclusion, we expected to provide a more convenient cloning method, as well as enable faster generation of DNA clones, which would be well applied in the less equipped laboratories.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 1","pages":"21 - 27"},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49531031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats 海藻糖对衰老大鼠肾脏NFE2L2、过氧化氢酶和超氧化物歧化酶的影响

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/mbrc.2022.42014.1688

Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi

{"title":"Effects of trehalose on NFE2L2, catalase, and superoxide dismutase in the kidney of aged rats","authors":"Yaser Hozhabri, Asie Sadeghi, Mahdieh Nazari-Robati, Faegheh Bahri, Fouzieh Salimi, M. Abolhassani, A. Mohammadi","doi":"10.22099/mbrc.2022.42014.1688","DOIUrl":"https://doi.org/10.22099/mbrc.2022.42014.1688","url":null,"abstract":"Aging is associated with an increase in oxidative stress, which damages organs such as the kidney. Trehalose has abundant beneficial activities including antioxidative effects. This study aimed to investigate the effects of trehalose on several antioxidant parameters of the aged kidney. Wistar rats were divided into three groups: young (4 months), aged (24 months), and aged-trehalose. The third group was treated with 2% trehalose for one month. The expression of target genes and enzyme activities in the kidney of the animals were evaluated by quantitative polymerase chain reaction (qPCR) and enzyme colorimetric procedures, respectively. Protein levels of NFE2L2 showed a 50% reduction in aged rats compared to young rats (P<0.001), which was restored by trehalose intervention. In addition, the activity and mRNA levels of catalase (CAT) increased in aged rats while treatment with trehalose reversed this trend. On the other hand, superoxide dismutase (SOD) activity was reduced in the kidneys of aged rats but was not affected by trehalose intervention .It is concluded that trehalose supplementation alleviates the antioxidant system impairments in the kidneys of aged rats. However, further investigations are needed to thoroughly describe the antioxidative impacts of trehalose on the kidneys during aging.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 1","pages":"29 - 36"},"PeriodicalIF":1.6,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42857164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toward a better understanding of phylogenetic relationships within Conringieae (Brassicaceae) 更好地了解芸苔科锥属植物的系统发育关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-03-01 DOI: 10.22099/MBRC.2022.42767.1709

A. Khosravi, Atena Eslami-Farouji, Atiqullah Sultani-Ahmadzai, S. Mohsenzadeh

引用次数: 5

Identification of Bordetella bronchiseptica in the throat and nose of dogs and cats by PCR. 用聚合酶链反应(PCR)鉴定犬、猫咽喉和鼻腔中的支气管脓毒杆菌。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43873.1755

Mohammad Tabatabaei, Hamid Reza Rohani

引用次数: 0

Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate. 推测产细菌素分离物杜兰肠球菌NT21的基因组分析。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44088.1760

Nashwa Tarek, Ahmed O El-Gendy, Ahmed S Khairalla, Medhat Abdel-Fattah, Eman Tawfik, Ahmed F Azmy

{"title":"Genomic analysis of Enterococcus durans NT21, a putative bacteriocin-producing isolate.","authors":"Nashwa Tarek, Ahmed O El-Gendy, Ahmed S Khairalla, Medhat Abdel-Fattah, Eman Tawfik, Ahmed F Azmy","doi":"10.22099/mbrc.2022.44088.1760","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44088.1760","url":null,"abstract":"Enterococcus species are a long-standing and non-pathogenic commensal bacterium, representing an important part of the normal. Enterococcus durans is a rarely isolated species from animals and humans, and it was a tiny constituent of human oral cavity and animal intestinal flora, as well as animal-derived foods, particularly dairy products. This study evaluated the security of our strain E. durans NT21 by using whole-genome sequencing (WGS), physicochemical features, and antimicrobial activity. The complete genomic of our strain Enterococcus durans NT21was sequenced and analyzed by using several bioinformatics tools to identify bacteriocin genes, virulence genes, antibiotic resistance genes, Crispr-Cas and pathogenicity islands. The results showed that our strain NT21 lacks the presence of virulence genes, pathogenicity islands, plasmids and has only two antibiotic resistance genes. On the other hand, it produces three bacteriocin-like inhibitory substances (Enterolysin A, P and L50a). It has six gene-encoded Crisper-Cas and one cluster Crispr-Cas gene. According to our findings, E. durans NT21 is a possible probiotic strain that is safe for both human and animal use.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"143-153"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer. 脂联素rs2241766和rs266729多态性与甲状腺乳头状癌风险的相关性

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.43012.1714

Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari

{"title":"Correlation between adiponectin rs2241766 and rs266729 polymorphisms and risk of papillary thyroid cancer.","authors":"Mohsen Maleki, Mansour Karajibani, Mohsen Sarvani, Farzaneh Montazerifar, Saeedeh Salimi, Zahra Heidari","doi":"10.22099/mbrc.2022.43012.1714","DOIUrl":"https://doi.org/10.22099/mbrc.2022.43012.1714","url":null,"abstract":"About 60-80% of thyroid cancer (TC) cases are papillary thyroid cancer (PTC). Studies have shown that serum adiponectin levels are inversely related to the risk of TC and PTC. Aim of the present study was to evaluate the association between adiponectin rs2241766 and rs266729 polymorphisms and risk of PTC. 122 PTC patients and 128 healthy subjects were enrolled in the study. PCR-RFLP and ARMS-PCR methods were used for genotype analysis. The rs266729 polymorphism did not correlate with risk of PTC. As regard rs2241766 polymorphism, the frequency of the GG genotype did not have a significant difference between the two groups, although, PTC cases showed higher frequency of GT genotype compared to controls (OR=2.87, 95% CI=1.56-5.28, P=0.001). We observed a significant association between adiponectin rs2241766 polymorphism and PTC, however, our result showed no significant relationship between adiponectin rs266729 polymorphism and risk of PTC.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 3","pages":"113-118"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9661673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10592099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2. SARS-CoV-2刺突、包膜、膜蛋白和ssRNA功能单元的胁迫适应特征

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44594.1777

Aniket Sarkar, Anindya Sundar Panja

{"title":"Stress adaptation signature into the functional units of spike, envelope, membrane protein and ssRNA of SARS-CoV-2.","authors":"Aniket Sarkar, Anindya Sundar Panja","doi":"10.22099/mbrc.2022.44594.1777","DOIUrl":"https://doi.org/10.22099/mbrc.2022.44594.1777","url":null,"abstract":"Pandemic coronavirus causes respiratory, enteric and sometimes neurological diseases. Proteome data of individual coronavirus strains were already reported. Here we investigated of SARS-CoV-2 ssRNA and protein of spike, envelope and membrane to determine stress adaptation profile. Thermodynamic properties, Physicochemical behaviour and, amino acid composition along with their RMSD value was analysed. Thermodynamic index of SARS-CoV2 spike, envelope and membrane ssRNA is unstable in higher temperature. Presence of higher proportion of polar with positive and negative charged amino acid residues into spike (S), envelope (E) and membrane (M) protein indicate the lower stress adaptability pattern. Our study represented several unstable pockets into S, E and M proteins of SARS-CoV-2 against different abiotic stresses, specifically higher in spike protein. Contact with heat through solvent may denature the architectural network of SARS-CoV-2 spike, envelope and membrane ssRNA and structural protein. The stress instability index of SARS-CoV-2 and the interactome profile of its transmembrane proteins may help to reveal novel factors for inhibiting SARS-CoV-2 growth.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"11 4","pages":"155-166"},"PeriodicalIF":1.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9905751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis and prevalence of Delta hepatitis among HBsAg carriers in Afghanistan. 阿富汗乙肝表面抗原携带者丁型肝炎的系统发育分析和流行情况。

IF 1.6

Molecular Biology Research Communications Pub Date : 2022-01-01 DOI: 10.22099/mbrc.2022.44692.1780

Abbas Ali Husseini, Mehran Rostamzadeh

引用次数: 0