Molecular Biology Research Communications最新文献_第6页

Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. 伊朗南部设拉子犬肝虫的检测及其对血液学改变的影响。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47151.1821

Ehsan Zoaktafi, Hassan Sharifiyazdi, Nooshin Derakhshandeh, Farnoosh Bakhshaei-Shahrbabaki

{"title":"Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations.","authors":"Ehsan Zoaktafi, Hassan Sharifiyazdi, Nooshin Derakhshandeh, Farnoosh Bakhshaei-Shahrbabaki","doi":"10.22099/mbrc.2023.47151.1821","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47151.1821","url":null,"abstract":"Canine hepatozoonosis is a tick-transmitted apicomplexan infection caused by two species of Hepatozoon, H. canis, and H. americanum. The present research aimed at detection of Hepatozoon spp. in dogs and its effects on hematological alterations. Blood samples were taken from 108 dogs to assess Hepatozoon spp. Phylogenetic analysis was performed based on the 18S rDNA marker by PCR assay and Giemsa-stained blood smear examination. Of the 108 blood samples of dogs tested in the present study, eight (7.40%, 95% CI: 3.25-14.07%) were positive by the Hepatozoon-specific PCR assay. However, in the microscopic examination, only one sample (0.93%) was positive. All of the sequenced samples were H. canis. The Hepatozoon sequences obtained from PCR amplicons in the canine-positive cases exhibited 100% similarity to each other and 98.47-100% similarity to other relevant sequences in GenBank. These findings represent the first molecular evidence of H. canis in dog populations in South Iran. Furthermore, according to the hematological analysis, significantly higher average numbers of neutrophils and lymphocytes were found in the infected group compared to the non-infected dogs. In this study, no statistically significant connection (P<0.05) was observed between H. canis infection and the examined risk factors.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 2","pages":"87-94"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the association of rs6983267 polymorphism of the CCAT2 gene with thyroid cancer susceptibility in the Iranian Azeri population. 评估CCAT2基因rs6983267多态性与伊朗阿塞拜疆人群甲状腺癌易感性的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47622.1839

Masoud Jabraili, Solmaz Moniri-Javadhesari, Nasser Pouladi, Mohammad Ali Hosseinpour-Feizi

{"title":"Evaluating the association of rs6983267 polymorphism of the CCAT2 gene with thyroid cancer susceptibility in the Iranian Azeri population.","authors":"Masoud Jabraili, Solmaz Moniri-Javadhesari, Nasser Pouladi, Mohammad Ali Hosseinpour-Feizi","doi":"10.22099/mbrc.2023.47622.1839","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47622.1839","url":null,"abstract":"Thyroid cancer is the most common malignancy of the endocrine system. LncRNAs play critical role in various cellular processes and are associated with several diseases. CCAT2 is a lncRNA molecule overexpressed in thyroid cancer. Single nucleotide polymorphisms in CCAT2 gene can cause changes in the structure and function of CCAT2 transcripts and susceptibility to several diseases. This study aimed to evaluate the association of rs6983267 in CCAT2 gene with thyroid cancer susceptibility in the Azeri population of Iran. In this \"case-control\" study, genomic DNA was extracted from peripheral blood of 102 individuals affected by thyroid cancer and 103 healthy individuals as controls. Genotyping was performed using TETRA-ARMS polymerase chain reaction. Statistical analysis showed no significant association between genotypes and/or alleles with the occurrence of thyroid cancer in the studied population, patients' gender, and tumor type. Nevertheless, we found that the allelic and genotypic distribution of this SNP was associated with the size of thyroid tumors in patients. It is assumed that investigating more individuals from both case and control group may further determine the genotypic and allelic frequencies of this SNP locus in Iranian-Azeri population.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 3","pages":"127-131"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study. 用套式PCR检测前列腺癌症和良性前列腺增生组织样本中的人类BKV大T基因组：一项病例对照研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47537.1836

Narges Tavassoli, Arastoo Vojdani, Sara Salimi-Namin, Majid Khadem-Rezaiyan, Mahmoudreza Kalantari, Masoud Youssefi

{"title":"Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study.","authors":"Narges Tavassoli, Arastoo Vojdani, Sara Salimi-Namin, Majid Khadem-Rezaiyan, Mahmoudreza Kalantari, Masoud Youssefi","doi":"10.22099/mbrc.2023.47537.1836","DOIUrl":"10.22099/mbrc.2023.47537.1836","url":null,"abstract":"Human BK polyomavirus (BKPyV) is a latent infectious agent in the genitourinary tract associated with hemorrhagic cystitis and nephropathy. This virus can be a risk factor for various human malignancies, including prostate cancer (PCa). It may contribute to prostate cancer development, as it demonstrates oncogenic properties by encoding oncoproteins. This study assessed the prevalence of this virus in benign and malignant prostate tissues. Between 2009 and 2019, 49 formalin-fixed paraffin-embedded (FFPE) PCa and 49 benign prostatic hyperplasia (BPH) samples were gathered from the pathology department of a tertiary care university hospital. They were used as cases and controls, respectively. After deparaffinization and DNA extraction, nested PCR was applied to identify the BKPyVgp5 gene (LTAg) using inner and outer primers. The nested PCR showed a 278-bp bond corresponding to the BKPyVgp5 genome (LTAg) in 53.1% (26/49) of PCa and 14.3% (7/49) of BPH (p<0.001). The presence of BKV was significantly associated with an increased risk of PCa development (OR=6.78, 95% CI=2.55-18.02, p<0.001). The BKV LTAg gene was significantly more prevalent in PCa samples than in BPH samples. These results demonstrate the presence of the virus in prostate cancer tissues.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 4","pages":"149-154"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54230206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mimicry and COVID-19: Potential implications for global fertility. 分子拟态和 COVID-19：对全球生育率的潜在影响。

IF 1.5

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47122.1819

Custer C Deocaris, Malona V Alinsug

引用次数: 0

Сytotoxic effect of CAR-T cells against modified MCF-7 breast cancer cell line. CAR-T细胞对改良MCF-7乳腺癌症细胞系的细胞毒性作用。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47125.1820

Aigul Kh Valiullina, Ekaterina A Zmievskaya, Irina A Ganeeva, Margarita N Zhuravleva, Ekaterina E Garanina, Albert A Rizvanov, Alexey V Petukhov, Emil R Bulatov

引用次数: 0

Structural arrangement of the active back-to-back dimer in N-glycosylated ErbB receptors is regulated by heterodimerization. n -糖基化ErbB受体中活性背对背二聚体的结构排列受异源二聚化调节。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47147.1822

Romina Mashayekh-Poul, Maryam Azimzadeh-Irani, Seyedeh Zeinab Masoomi-Nomandan

{"title":"Structural arrangement of the active back-to-back dimer in N-glycosylated ErbB receptors is regulated by heterodimerization.","authors":"Romina Mashayekh-Poul, Maryam Azimzadeh-Irani, Seyedeh Zeinab Masoomi-Nomandan","doi":"10.22099/mbrc.2023.47147.1822","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47147.1822","url":null,"abstract":"The human epidermal growth factor receptor (EGFR/ErbB) family consists of four members (ErbB1-4) and belongs to the superfamily of receptor tyrosine kinases (RTKs). The ErbB family members participate in multiple cellular pathways and are the key players in several cancers (brain, breast, lung etc.). Activation of these family members depends on their extracellular domains forming back-to-back hetero/homo dimers. Moreover, dimers are glycosylated, which is a crucial post-translational modification that affects the conformation and function of the protein. Here, molecular modeling and molecular docking are used to comprehensively investigate the dimerization mechanism in glycosylated back-to-back active dimer formation in the entire ErbB receptors for the first time. Results showed that 21 out of 37 clusters of active back-to-back dimers formed by all family members are through heterodimerization. Including; ErbB1-ErbB3/ErbB4, ErbB2-ErbB3/ErbB4 and ErbB3-ErbB4. Ranking ErbB2-ErbB3 as the most stabilized back-to-back dimeric construct. While glycan arrangements favor both homo/hetero dimerization at the dimeric interfaces, it promotes heterodimerization by stabilizing and packing the ligand binding sites of EGFR and ErbB2 respectively. These findings pave the path to future heterodimeric interface/glycan targeting rational anti-cancer drug designs for ErbB receptors.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 3","pages":"95-107"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The molecular characteristics of colorectal cancer: Impact of Ibuprofen and hyperthermia. 结直肠癌的分子特征:布洛芬和热疗的影响。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.45296.1802

Farzaneh Zarghampoor, Behnaz Valibeigi, Abbas Behzad-Behbahani

{"title":"The molecular characteristics of colorectal cancer: Impact of Ibuprofen and hyperthermia.","authors":"Farzaneh Zarghampoor, Behnaz Valibeigi, Abbas Behzad-Behbahani","doi":"10.22099/mbrc.2023.45296.1802","DOIUrl":"https://doi.org/10.22099/mbrc.2023.45296.1802","url":null,"abstract":"Despite various treatment options available for colorectal cancer, the survival rates for patients remain low. This study investigated the effects of hyperthermia and Ibuprofen on human colorectal adenocarcinoma cells (HT-29) viability, proliferation, and gene expression related to tumor suppression, Wnt signaling pathways, proliferation, and apoptosis The cells were exposed to hyperthermia at 42 or 43°C for 3 hours or Ibuprofen at different concentrations (700-1500 μM), and the effects were analyzed through MTT assay, trypan blue staining, and quantitative Real-time PCR. The study used quantitative Real-time PCR (qRT-PCR) to evaluate the effect of hyperthermia and Ibuprofen on the expression of various genes associated with tumor suppression, proliferation, Wnt signaling pathway, and apoptosis. The results revealed that hyperthermia caused a minor reduction in the viability and proliferation of HT-29 cells, but the decrease was not statistically significant (P<0.05). On the other hand, Ibuprofen caused a concentration-dependent decrease in the viability and proliferation of HT-29 cells. Both hyperthermia and Ibuprofen reduced the expression of WNT1, CTNNB1, BCL2, and PCNA genes, and increased the expression of KLF4, P53, and BAX genes. However, the changes in gene expression were not statistically significant in cells treated with hyperthermia. The findings suggest that Ibuprofen is more effective in reducing cancer cell proliferation by promoting apoptosis and inhibiting the Wnt signaling pathway than hyperthermia, which had some impact but was not statistically significant. The study highlights the potential of Ibuprofen as a targeted therapy for colorectal cancer.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 1","pages":"17-25"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of RORA (RAR-Related Orphan Receptor Alpha) receptors on litter size in Akkaraman sheep breed. rar相关孤儿受体α受体对阿卡拉曼羊产仔数的影响。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47336.1827

Serbulent Yiğit, Selim Kul, Recai Aci, Adem Keskin, Tuğçe Tuygun, Esra Duman

{"title":"The effect of RORA (RAR-Related Orphan Receptor Alpha) receptors on litter size in Akkaraman sheep breed.","authors":"Serbulent Yiğit, Selim Kul, Recai Aci, Adem Keskin, Tuğçe Tuygun, Esra Duman","doi":"10.22099/mbrc.2023.47336.1827","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47336.1827","url":null,"abstract":"In this study, the relationship between RORA 23bp indel genotype and allele frequency with twin pregnancy, fertility, live weight and milk yield in 106 female Akkaraman ewes raised in Elazığ province was investigated. In the study conducted in Elâzığ province, 10ml milk was collected from 106 Akkaraman sheep and DNA was extracted from these milk. In RORA 23bp indel genotype frequency, DD genotype was found more than ID and II genotypes and RORA 23bp indel ın allele frequency, the D allele was found to be higher than the I allele. In both the first and second parity, the twinning rate was found to be lower. In both the first and second parity, the twinning rate was higher in the DD genotype, and it was observed that this genotype promınated mıddle lıvestock weıght and mılk yıeld. According to the results of our study, mutations in the RORA gene, which is a gene affecting reproductive efficiency in sheep, do not have a positive effect on fertility and twinning rate in Akkaraman sheep. To sum, this study provided theoretical references for the comprehensively research of the function of RORA gene and the breeding of Akkaraman Sheep. The 23-bp indel variants can be considered as molecular markers for litter size of sheep for marker-assisted selection breeding.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 3","pages":"109-115"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic alterations in MED12 promote castration-resistant prostate cancer through modulation of GLI3 signaling. MED12基因改变通过调节GLI3信号传导促进去势抵抗性前列腺癌。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47346.1828

Thu Minh Duong, Mariana Araujo Rincon, Nishanth Myneni, Marieke Burleson

{"title":"Genetic alterations in MED12 promote castration-resistant prostate cancer through modulation of GLI3 signaling.","authors":"Thu Minh Duong, Mariana Araujo Rincon, Nishanth Myneni, Marieke Burleson","doi":"10.22099/mbrc.2023.47346.1828","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47346.1828","url":null,"abstract":"Prostate cancer is a disease that depends on androgenic stimulation and is thus commonly treated with androgen deprivation therapy (ADT). ADT is highly successful initially; however, patients inevitably relapse at which point the cancer grows independently of androgens and is termed castration-resistant prostate cancer (CRPC). CRPC develops through various mechanisms, one of these being crosstalk of the androgen receptor (AR) signaling pathway with other signaling pathways. Congruently, prior work has shown that androgen deprivation induces SHH signaling, which subsequently promotes activation of AR-dependent gene expression to promote cell growth. Mechanistically, this crosstalk involves a physical interaction between AR and components of SHH signaling, specifically proteins of the GLI transcription factor family. These findings thus suggest that activation of SHH signaling could promote the recurrence of cell growth in the absence of androgens to ultimately lead to progression towards CRPC. In this study, we have investigated this mechanism in a subset of prostate cancer that harbors genetic alterations within the Mediator subunit 12 (MED12). We found that loss of MED12 promotes the expression of GLI3 target genes which subsequently drives excessive cell growth in the absence of androgens. Thus, we conclude that genetic alterations within MED12 promote CRPC through hyperactivated GLI3 dependent sonic hedgehog signaling.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 2","pages":"63-70"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9906749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between CX3CR1 rs3732378 polymorphism and neovascular age-related macular degeneration in a sample of Algerian population. 阿尔及利亚人群样本中CX3CR1 rs3732378多态性与新生血管性年龄相关性黄斑变性之间的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.46767.1809

Ahlem Messal, Ghania Abid, Meriem Abdi, Aicha Idder, Naima Meroufel, Faouzia Zemani-Fodil, Mostefa Fodil

{"title":"Association between CX3CR1 rs3732378 polymorphism and neovascular age-related macular degeneration in a sample of Algerian population.","authors":"Ahlem Messal, Ghania Abid, Meriem Abdi, Aicha Idder, Naima Meroufel, Faouzia Zemani-Fodil, Mostefa Fodil","doi":"10.22099/mbrc.2023.46767.1809","DOIUrl":"https://doi.org/10.22099/mbrc.2023.46767.1809","url":null,"abstract":"Neovascular age-related macular degeneration (nAMD) is a progressive ocular disease, responsible for central visual loss and blindness in elderly population. Increase data demonstrate that genetic factors play an important role in pathogenesis process of this disease. The aim of this study is to investigate the association between rs3732378 polymorphism in CX3CR1 gene and nAMD in a sample of Algerian patients. This case-control study consisted of 72 patients with nAMD and 124 control subjects. DNA of participants was extracted using salting out method. Genotyping was carried out using the TaqMan real-time polymerase chain reaction method. Statistical analysis was performed by SPSS.21.0. The prevalence of the risk genotype AA was higher in the nAMD group than in control group (OR=5.02, 95% CI=1.44-17.4, P=0.011). In our sample of Algerian patients, the rs3732378 polymorphism is associated with nAMD. This result may support the role of CX3CR1 gene in the pathogenesis of nAMD.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 2","pages":"57-62"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0