Molecular Biology Research Communications最新文献_第4页

Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion. 伊朗Mesobuthus eupeus蝎两种抗癌毒素嵌合肽MeICT/IMe-AGAP的设计与构建

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.46450.1804

Razieh Seifi, Hoda Ayat, Ali Mohammad Ahadi

{"title":"Design and construction of a chimeric peptide, MeICT/IMe-AGAP, from two anti-cancer toxins of Iranian Mesobuthus eupeus scorpion.","authors":"Razieh Seifi, Hoda Ayat, Ali Mohammad Ahadi","doi":"10.22099/mbrc.2023.46450.1804","DOIUrl":"https://doi.org/10.22099/mbrc.2023.46450.1804","url":null,"abstract":"Scorpion venom contains various toxin peptides with pharmacological and biological properties. Scorpion toxins specifically interact with membrane ion channels which play key roles in progression of cancer. Therefore, scorpion toxins have received special attention for targeting cancer cells. Two new toxins MeICT and IMe-AGAP, isolated from Iranian yellow scorpion, Mesobuthus eupeus, interact specifically with chloride and sodium channels, respectively. Anti-cancer properties of MeICT and IMe-AGAP have been determined before, in addition they show 81 and 93% similarity with two well-known anti-cancer toxins, CTX and AGAP, respectively. The aim of this study was construction of a fusion peptide MeICT/IMe-AGAP to target different ion channels involved in cancer progression. Design and structure of the fusion peptide were investigated by bioinformatics studies. Two fragments encoding MeICT and IMe-AGAP were fused using overlapping primers by SOEing-PCR. MeICT/IMe-AGAP chimeric fragment was cloned into pET32Rh vector, expressed in Escherichia coli host and analyzed by SDS-PAGE. The in silico studies showed that chimeric peptide with GPSPG linker preserved the three-dimensional structure of both peptides and can be functional. Due to the high expression of chloride and sodium channels in various cancer cells, MeICT/IMe-AGAP fusion peptide can be used as an effective agent to target both channels in cancers, simultaneously.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 1","pages":"27-36"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9845449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genetic polymorphisms at the promoter region of HLA-DQB1 gene, creating responsive elements for NF1/CTF and converting the TFII-D binding site to GR-alpha. HLA-DQB1基因启动子区域的遗传多态性，产生NF1/CTF的应答元件，并将TFII-D结合位点转化为gr - α。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.46890.1813

Khyber Saify

{"title":"The genetic polymorphisms at the promoter region of HLA-DQB1 gene, creating responsive elements for NF1/CTF and converting the TFII-D binding site to GR-alpha.","authors":"Khyber Saify","doi":"10.22099/mbrc.2023.46890.1813","DOIUrl":"https://doi.org/10.22099/mbrc.2023.46890.1813","url":null,"abstract":"Human leukocyte antigen-DQB1 (HLA-DQB1, OMIM: 604305) is the human major histocompatibility complex (MHC) system. HLA genes are classified into three classes (I, II, and III). The HLA-DQB1 belongs to class II, is mainly involved in the actions of the human immune system and plays a fundamental role in donor-recipient matching in transplantation and can be associated with most autoimmune diseases. In this study, the potential influence(s) of the G-71C (rs71542466) and T-80C (rs9274529) genetic polymorphisms were investigated. These polymorphisms, located in the HLA-DQB1 promoter region, have a significant frequency in the world population. The online software ALGGEN-PROMO.v8.3 was used in this work. The results indicate that the C allele at the -71 position actually creates a new potential binding site for NF1/CTF and the C allele at the -80 position changes the TFII-D binding site into a GR-alpha response element. The NF1/CTF plays the role of activator and the GR-alpha is the inhibitor; thus, according to the roles of these transcription factors, it is suggested that the above-mentioned polymorphisms alter the expression levels of HLA-DQB1. Therefore, this genetic variation is associated with autoimmune diseases; however, this cannot be generalized because this is the first report and more studies are needed in the future.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 1","pages":"51-55"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9490169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction. 含dppm配体的氟化环铂(II)配合物对癌细胞的体外培养和计算机预测的毒性和自噬作用。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.44705.1781

Zahra Kamalzade, Elham Hoveizi, Masood Fereidoonnezhad

{"title":"Toxicity and autophagy effects of fluorinated cycloplatinated(II) complex bearing dppm ligand on cancer cells in in-vitro culture and in-silico prediction.","authors":"Zahra Kamalzade, Elham Hoveizi, Masood Fereidoonnezhad","doi":"10.22099/mbrc.2023.44705.1781","DOIUrl":"https://doi.org/10.22099/mbrc.2023.44705.1781","url":null,"abstract":"Toxicity and autophagy effects of a new complex of platinum II (CPC) were evaluated on HeLa cells cultured on a PCL/gelatin electrospinning scaffold. HeLa cells were treated with CPC on the first, third, and fifth days and the concentration of IC50 was determined. The autophagic and apoptotic effects of CPC were examined by MTT assay, Acridine Orange, Giemsa, DAPI, MDC, real-time PCR, Western blot testing, and molecular docking. The cell viability was obtained on days 1, 3, and 5 as much as 50, 7.28, and 19%, respectively with a concentration of IC50 (100μM) of CPC. The staining results indicated that the treatment of HeLa cells with CPC had antitumor and autophagic effects. Results of RT-PCR showed that the expression of BAX, BAD, P53, and LC3 genes was significantly increased in the sample treated with IC50 concentration compared to the control sample whereas the expression of BCL2, mTOR, and ACT genes in cells was significantly decreased compared to the control group. Also, these results were confirmed by Western blotting. The data indicated the induction of apoptotic death and autophagy in the studied cells. The new compound of CPC has antitumor effects.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 1","pages":"37-49"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9845452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations. 伊朗南部设拉子犬肝虫的检测及其对血液学改变的影响。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47151.1821

Ehsan Zoaktafi, Hassan Sharifiyazdi, Nooshin Derakhshandeh, Farnoosh Bakhshaei-Shahrbabaki

{"title":"Detection of Hepatozoon spp. in dogs in Shiraz, southern Iran and its effects on the hematological alterations.","authors":"Ehsan Zoaktafi, Hassan Sharifiyazdi, Nooshin Derakhshandeh, Farnoosh Bakhshaei-Shahrbabaki","doi":"10.22099/mbrc.2023.47151.1821","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47151.1821","url":null,"abstract":"Canine hepatozoonosis is a tick-transmitted apicomplexan infection caused by two species of Hepatozoon, H. canis, and H. americanum. The present research aimed at detection of Hepatozoon spp. in dogs and its effects on hematological alterations. Blood samples were taken from 108 dogs to assess Hepatozoon spp. Phylogenetic analysis was performed based on the 18S rDNA marker by PCR assay and Giemsa-stained blood smear examination. Of the 108 blood samples of dogs tested in the present study, eight (7.40%, 95% CI: 3.25-14.07%) were positive by the Hepatozoon-specific PCR assay. However, in the microscopic examination, only one sample (0.93%) was positive. All of the sequenced samples were H. canis. The Hepatozoon sequences obtained from PCR amplicons in the canine-positive cases exhibited 100% similarity to each other and 98.47-100% similarity to other relevant sequences in GenBank. These findings represent the first molecular evidence of H. canis in dog populations in South Iran. Furthermore, according to the hematological analysis, significantly higher average numbers of neutrophils and lymphocytes were found in the infected group compared to the non-infected dogs. In this study, no statistically significant connection (P<0.05) was observed between H. canis infection and the examined risk factors.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 2","pages":"87-94"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the association of rs6983267 polymorphism of the CCAT2 gene with thyroid cancer susceptibility in the Iranian Azeri population. 评估CCAT2基因rs6983267多态性与伊朗阿塞拜疆人群甲状腺癌易感性的关系

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47622.1839

Masoud Jabraili, Solmaz Moniri-Javadhesari, Nasser Pouladi, Mohammad Ali Hosseinpour-Feizi

{"title":"Evaluating the association of rs6983267 polymorphism of the CCAT2 gene with thyroid cancer susceptibility in the Iranian Azeri population.","authors":"Masoud Jabraili, Solmaz Moniri-Javadhesari, Nasser Pouladi, Mohammad Ali Hosseinpour-Feizi","doi":"10.22099/mbrc.2023.47622.1839","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47622.1839","url":null,"abstract":"Thyroid cancer is the most common malignancy of the endocrine system. LncRNAs play critical role in various cellular processes and are associated with several diseases. CCAT2 is a lncRNA molecule overexpressed in thyroid cancer. Single nucleotide polymorphisms in CCAT2 gene can cause changes in the structure and function of CCAT2 transcripts and susceptibility to several diseases. This study aimed to evaluate the association of rs6983267 in CCAT2 gene with thyroid cancer susceptibility in the Azeri population of Iran. In this \"case-control\" study, genomic DNA was extracted from peripheral blood of 102 individuals affected by thyroid cancer and 103 healthy individuals as controls. Genotyping was performed using TETRA-ARMS polymerase chain reaction. Statistical analysis showed no significant association between genotypes and/or alleles with the occurrence of thyroid cancer in the studied population, patients' gender, and tumor type. Nevertheless, we found that the allelic and genotypic distribution of this SNP was associated with the size of thyroid tumors in patients. It is assumed that investigating more individuals from both case and control group may further determine the genotypic and allelic frequencies of this SNP locus in Iranian-Azeri population.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 3","pages":"127-131"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9919996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study. 用套式PCR检测前列腺癌症和良性前列腺增生组织样本中的人类BKV大T基因组：一项病例对照研究。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47537.1836

Narges Tavassoli, Arastoo Vojdani, Sara Salimi-Namin, Majid Khadem-Rezaiyan, Mahmoudreza Kalantari, Masoud Youssefi

{"title":"Human BKV large T genome detection in prostate cancer and benign prostatic hyperplasia tissue samples by nested PCR: A case-control study.","authors":"Narges Tavassoli, Arastoo Vojdani, Sara Salimi-Namin, Majid Khadem-Rezaiyan, Mahmoudreza Kalantari, Masoud Youssefi","doi":"10.22099/mbrc.2023.47537.1836","DOIUrl":"10.22099/mbrc.2023.47537.1836","url":null,"abstract":"Human BK polyomavirus (BKPyV) is a latent infectious agent in the genitourinary tract associated with hemorrhagic cystitis and nephropathy. This virus can be a risk factor for various human malignancies, including prostate cancer (PCa). It may contribute to prostate cancer development, as it demonstrates oncogenic properties by encoding oncoproteins. This study assessed the prevalence of this virus in benign and malignant prostate tissues. Between 2009 and 2019, 49 formalin-fixed paraffin-embedded (FFPE) PCa and 49 benign prostatic hyperplasia (BPH) samples were gathered from the pathology department of a tertiary care university hospital. They were used as cases and controls, respectively. After deparaffinization and DNA extraction, nested PCR was applied to identify the BKPyVgp5 gene (LTAg) using inner and outer primers. The nested PCR showed a 278-bp bond corresponding to the BKPyVgp5 genome (LTAg) in 53.1% (26/49) of PCa and 14.3% (7/49) of BPH (p<0.001). The presence of BKV was significantly associated with an increased risk of PCa development (OR=6.78, 95% CI=2.55-18.02, p<0.001). The BKV LTAg gene was significantly more prevalent in PCa samples than in BPH samples. These results demonstrate the presence of the virus in prostate cancer tissues.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 4","pages":"149-154"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54230206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mimicry and COVID-19: Potential implications for global fertility. 分子拟态和 COVID-19：对全球生育率的潜在影响。

IF 1.5

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47122.1819

Custer C Deocaris, Malona V Alinsug

引用次数: 0

Сytotoxic effect of CAR-T cells against modified MCF-7 breast cancer cell line. CAR-T细胞对改良MCF-7乳腺癌症细胞系的细胞毒性作用。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47125.1820

Aigul Kh Valiullina, Ekaterina A Zmievskaya, Irina A Ganeeva, Margarita N Zhuravleva, Ekaterina E Garanina, Albert A Rizvanov, Alexey V Petukhov, Emil R Bulatov

引用次数: 0

Structural arrangement of the active back-to-back dimer in N-glycosylated ErbB receptors is regulated by heterodimerization. n -糖基化ErbB受体中活性背对背二聚体的结构排列受异源二聚化调节。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.47147.1822

Romina Mashayekh-Poul, Maryam Azimzadeh-Irani, Seyedeh Zeinab Masoomi-Nomandan

{"title":"Structural arrangement of the active back-to-back dimer in N-glycosylated ErbB receptors is regulated by heterodimerization.","authors":"Romina Mashayekh-Poul, Maryam Azimzadeh-Irani, Seyedeh Zeinab Masoomi-Nomandan","doi":"10.22099/mbrc.2023.47147.1822","DOIUrl":"https://doi.org/10.22099/mbrc.2023.47147.1822","url":null,"abstract":"The human epidermal growth factor receptor (EGFR/ErbB) family consists of four members (ErbB1-4) and belongs to the superfamily of receptor tyrosine kinases (RTKs). The ErbB family members participate in multiple cellular pathways and are the key players in several cancers (brain, breast, lung etc.). Activation of these family members depends on their extracellular domains forming back-to-back hetero/homo dimers. Moreover, dimers are glycosylated, which is a crucial post-translational modification that affects the conformation and function of the protein. Here, molecular modeling and molecular docking are used to comprehensively investigate the dimerization mechanism in glycosylated back-to-back active dimer formation in the entire ErbB receptors for the first time. Results showed that 21 out of 37 clusters of active back-to-back dimers formed by all family members are through heterodimerization. Including; ErbB1-ErbB3/ErbB4, ErbB2-ErbB3/ErbB4 and ErbB3-ErbB4. Ranking ErbB2-ErbB3 as the most stabilized back-to-back dimeric construct. While glycan arrangements favor both homo/hetero dimerization at the dimeric interfaces, it promotes heterodimerization by stabilizing and packing the ligand binding sites of EGFR and ErbB2 respectively. These findings pave the path to future heterodimeric interface/glycan targeting rational anti-cancer drug designs for ErbB receptors.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 3","pages":"95-107"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9922453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The molecular characteristics of colorectal cancer: Impact of Ibuprofen and hyperthermia. 结直肠癌的分子特征:布洛芬和热疗的影响。

IF 1.6

Molecular Biology Research Communications Pub Date : 2023-01-01 DOI: 10.22099/mbrc.2023.45296.1802

Farzaneh Zarghampoor, Behnaz Valibeigi, Abbas Behzad-Behbahani

{"title":"The molecular characteristics of colorectal cancer: Impact of Ibuprofen and hyperthermia.","authors":"Farzaneh Zarghampoor, Behnaz Valibeigi, Abbas Behzad-Behbahani","doi":"10.22099/mbrc.2023.45296.1802","DOIUrl":"https://doi.org/10.22099/mbrc.2023.45296.1802","url":null,"abstract":"Despite various treatment options available for colorectal cancer, the survival rates for patients remain low. This study investigated the effects of hyperthermia and Ibuprofen on human colorectal adenocarcinoma cells (HT-29) viability, proliferation, and gene expression related to tumor suppression, Wnt signaling pathways, proliferation, and apoptosis The cells were exposed to hyperthermia at 42 or 43°C for 3 hours or Ibuprofen at different concentrations (700-1500 μM), and the effects were analyzed through MTT assay, trypan blue staining, and quantitative Real-time PCR. The study used quantitative Real-time PCR (qRT-PCR) to evaluate the effect of hyperthermia and Ibuprofen on the expression of various genes associated with tumor suppression, proliferation, Wnt signaling pathway, and apoptosis. The results revealed that hyperthermia caused a minor reduction in the viability and proliferation of HT-29 cells, but the decrease was not statistically significant (P<0.05). On the other hand, Ibuprofen caused a concentration-dependent decrease in the viability and proliferation of HT-29 cells. Both hyperthermia and Ibuprofen reduced the expression of WNT1, CTNNB1, BCL2, and PCNA genes, and increased the expression of KLF4, P53, and BAX genes. However, the changes in gene expression were not statistically significant in cells treated with hyperthermia. The findings suggest that Ibuprofen is more effective in reducing cancer cell proliferation by promoting apoptosis and inhibiting the Wnt signaling pathway than hyperthermia, which had some impact but was not statistically significant. The study highlights the potential of Ibuprofen as a targeted therapy for colorectal cancer.","PeriodicalId":19025,"journal":{"name":"Molecular Biology Research Communications","volume":"12 1","pages":"17-25"},"PeriodicalIF":1.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10186857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0