Allele and genotype frequencies of β-lactoglobulin gene using PCR-RFLP in Algerian local cattle populations.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Nadjet Boushaba, Nacera Tabet-Aoul
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引用次数: 0

Abstract

Milk protein genetic polymorphisms are associated with economically important traits in dairy cattle. The objective of this study is to genotype a single nucleotide polymorphism (SNP) responsible for the amino acid changes in the beta-lactoglobulin (β-Lg) variants A and B on 85 unrelated DNA representing Algerian cattle populations: Chelifienne (28), Cheurfa (31) and Guelmoise (26). The method used is the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Genetic polymorphism was detected by digestion of PCR products amplified of exon II of β-Lg gene by with the endonuclease HaeIII enzyme. The results revealed that the amplified product was observed as 247 bp. Restriction digestion with HaeIII revealed three genotypes: AA, AB and BB. The genotypic frequencies of AA, AB and BB genotypes were 0.08, 0.41, 0.50; 0.08, 0.41, 0.50 and 0.01, 0.19, 0.56 in Chelifienne, Cheurfa and Guelmoise and respectively. Frequency of AA genotype was absent in Guelmoise population. Frequencies of A and B alleles were 0.29 and 0.71 in both Chelifienne and Cheurfa and 0.25 and 0.75 Guelmoise population. These results further confirm that Bos torus cattle are predominantly of β-Lactoglobulin B type. The Chi-square test at p-value < 0.05 results revealed that the Chelifienne and Cheurfa populations were in Hardy-Weinberg equilibrium and the results are not significant for the Guelmoise. This genetic information could be useful to estimate the effect of polymorphism on different milk production of Algerian bovine populations.

利用 PCR-RFLP 技术测定阿尔及利亚当地牛群中 β 乳球蛋白基因的等位基因和基因型频率。
乳蛋白基因多态性与奶牛的重要经济性状有关。本研究的目的是对代表阿尔及利亚牛种群的 85 个无关联 DNA 上的β-乳球蛋白(β-Lg)变体 A 和 B 的氨基酸变化负责的单核苷酸多态性(SNP)进行基因分型:Chelifienne(28)、Cheurfa(31)和 Guelmoise(26)。使用的方法是 PCR-RFLP(聚合酶链式反应-限制性片段长度多态性)。用内切酶 HaeIII 消化β-Lg 基因外显子 II 的 PCR 扩增产物,检测基因多态性。结果显示,扩增产物为 247 bp。用 HaeIII 进行限制性消化发现了三种基因型:AA、AB 和 BB:AA、AB 和 BB。在 Chelifienne、Cheurfa 和 Guelmoise,AA、AB 和 BB 基因型的频率分别为 0.08、0.41、0.50;0.08、0.41、0.50 和 0.01、0.19、0.56。Guelmoise人群中没有AA基因型。A和B等位基因在Chelifienne和Cheurfa种群中的频率分别为0.29和0.71,在Guelmoise种群中的频率分别为0.25和0.75。这些结果进一步证实,Bos torus 牛主要属于 β-乳球蛋白 B 型。P值<0.05的Chi-square检验结果显示,Chelifienne种群和Cheurfa种群处于Hardy-Weinberg平衡状态,而Guelmoise种群的结果并不显著。这些遗传信息有助于估计多态性对阿尔及利亚牛群不同产奶量的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Biology Research Communications
Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
0.00%
发文量
12
期刊介绍: “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.
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