Nature Plants最新文献_第8页

Loving salt 爱盐

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-17 DOI: 10.1038/s41477-024-01740-w

Guillaume Tena

引用次数: 0

Cleave and Rescue gamete killers create conditions for gene drive in plants 裂解和拯救配子杀手为植物基因驱动创造条件

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-17 DOI: 10.1038/s41477-024-01701-3

Georg Oberhofer, Michelle L. Johnson, Tobin Ivy, Igor Antoshechkin, Bruce A. Hay

{"title":"Cleave and Rescue gamete killers create conditions for gene drive in plants","authors":"Georg Oberhofer, Michelle L. Johnson, Tobin Ivy, Igor Antoshechkin, Bruce A. Hay","doi":"10.1038/s41477-024-01701-3","DOIUrl":"10.1038/s41477-024-01701-3","url":null,"abstract":"Gene drive elements promote the spread of linked traits and can be used to change the composition or fate of wild populations. Cleave and Rescue (ClvR) drive elements sit at a fixed chromosomal position and include a DNA sequence-modifying enzyme such as Cas9/gRNAs that disrupts endogenous versions of an essential gene and a recoded version of the essential gene resistant to cleavage. ClvR spreads by creating conditions in which those lacking ClvR die because they lack functional versions of the essential gene. Here we demonstrate the essential features of the ClvR gene drive in the plant Arabidopsis thaliana through killing of gametes that fail to inherit a ClvR that targets the essential gene YKT61. Resistant alleles, which can slow or prevent drive, were not observed. Modelling shows plant ClvRs are robust to certain failure modes and can be used to rapidly drive population modification or suppression. Possible applications are discussed. Gene drive elements spread linked traits and can be used to change the composition or fate of populations. Here Oberhofer and colleagues engineer gene drive in the plant Arabidopsis thaliana. Applications include genetic biocontrol and conservation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Driving evolution in wild plants 推动野生植物的进化

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-17 DOI: 10.1038/s41477-024-01723-x

Paul Neve, Luke Barrett

引用次数: 0

Overriding Mendelian inheritance in Arabidopsis with a CRISPR toxin–antidote gene drive that impairs pollen germination 用 CRISPR 毒素-抗毒素基因驱动技术超越拟南芥的孟德尔遗传，使花粉萌发受阻

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-17 DOI: 10.1038/s41477-024-01692-1

Yang Liu, Bingke Jiao, Jackson Champer, Wenfeng Qian

{"title":"Overriding Mendelian inheritance in Arabidopsis with a CRISPR toxin–antidote gene drive that impairs pollen germination","authors":"Yang Liu, Bingke Jiao, Jackson Champer, Wenfeng Qian","doi":"10.1038/s41477-024-01692-1","DOIUrl":"10.1038/s41477-024-01692-1","url":null,"abstract":"Synthetic gene drives, inspired by natural selfish genetic elements and transmitted to progeny at super-Mendelian (>50%) frequencies, present transformative potential for disseminating traits that benefit humans throughout wild populations, even facing potential fitness costs. Here we constructed a gene drive system in plants called CRISPR-Assisted Inheritance utilizing NPG1 (CAIN), which uses a toxin–antidote mechanism in the male germline to override Mendelian inheritance. Specifically, a guide RNA–Cas9 cassette targets the essential No Pollen Germination 1 (NPG1) gene, serving as the toxin to block pollen germination. A recoded, CRISPR-resistant copy of NPG1 serves as the antidote, providing rescue only in pollen cells that carry the drive. To limit potential consequences of inadvertent release, we used self-pollinating Arabidopsis thaliana as a model. The drive demonstrated a robust 88–99% transmission rate over two successive generations, producing minimal resistance alleles that are unlikely to inhibit drive spread. Our study provides a strong basis for rapid genetic modification or suppression of outcrossing plant populations. Researchers have developed a synthetic gene drive in Arabidopsis, demonstrating a proof of concept for biasing inheritance that could enable rapid genetic modifications or population suppression in wild plants.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141333728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phragmoplast expansion requires α-Aurora 膈细胞的扩展需要α-极光

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-13 DOI: 10.1038/s41477-024-01731-x

Raphael Trösch

引用次数: 0

Author Correction: Structure of plant photosystem I in a native assembly state defines PsaF as a regulatory checkpoint 作者更正：原生装配状态下植物光系统 I 的结构将 PsaF 定义为调控检查点。

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-10 DOI: 10.1038/s41477-024-01737-5

Andreas Naschberger, Mariia Fadeeva, Daniel Klaiman, Anna Borovikova-Sheinker, Ido Caspy, Nathan Nelson, Alexey Amunts

引用次数: 0

Reply to: Critical comment on the assumptions leading to 24-chain microfibrils in wood 答复对导致木材中出现 24 链微纤维的假设的批判性评论

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-07 DOI: 10.1038/s41477-024-01727-7

Hwan-Ching Tai, Cheng-Si Tsao, Jer-Horng Lin

引用次数: 0

Author Correction: Designing a synthetic moss genome using GenoDesigner 作者更正：使用 GenoDesigner 设计合成苔藓基因组。

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-07 DOI: 10.1038/s41477-024-01734-8

Wenfei Yu, Shuo Zhang, Shijun Zhao, Lian-ge Chen, Jie Cao, Hao Ye, Jianbin Yan, Qiao Zhao, Beixin Mo, Ying Wang, Yuling Jiao, Yingxin Ma, Xiaoluo Huang, Wenfeng Qian, Junbiao Dai

引用次数: 0

Genetic editing of grain size genes enables fully mechanized hybrid rice breeding 谷粒大小基因的基因编辑实现了杂交水稻的全机械化育种

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-06 DOI: 10.1038/s41477-024-01722-y

引用次数: 0

Author Correction: Local brassinosteroid biosynthesis enables optimal root growth 作者更正：局部铜绿素类固醇生物合成可实现最佳根系生长。

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-06-05 DOI: 10.1038/s41477-024-01732-w

Nemanja Vukašinović, Yaowei Wang, Isabelle Vanhoutte, Matyáš Fendrych, Boyu Guo, Miroslav Kvasnica, Petra Jiroutová, Jana Oklestkova, Miroslav Strnad, Eugenia Russinova

引用次数: 0