Nature Plants最新文献_第8页

Nuclear-localized pyruvate kinases control phosphorylation of histone H3 on threonine 11 核定位丙酮酸激酶控制组蛋白 H3 苏氨酸 11 上的磷酸化

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01821-w

Pengcheng Hu, Yanmei Xu, Yanhua Su, Yuxin Wang, Yan Xiong, Yong Ding

{"title":"Nuclear-localized pyruvate kinases control phosphorylation of histone H3 on threonine 11","authors":"Pengcheng Hu, Yanmei Xu, Yanhua Su, Yuxin Wang, Yan Xiong, Yong Ding","doi":"10.1038/s41477-024-01821-w","DOIUrl":"10.1038/s41477-024-01821-w","url":null,"abstract":"Phosphorylation of histone H3 at threonine 11 (H3T11ph) affects transcription and chromosome stability. However, the enzymes responsible for depositing H3T11ph and the functions of H3T11ph in plants remain unknown. Here we report that in Arabidopsis thaliana, PYRUVATE KINASE 6 (PK6), PK7 and PK8 enter the nucleus under conditions of sufficient glucose and light exposure, where they interact with SWI2/SNF2-RELATED 1 COMPLEX 4 (SWC4) and phosphorylate H3 at threonine 11. Mutations in these kinases or knockdown of SWC4 resulted in FLC-dependent early flowering, short hypocotyls and short pedicels. Genome-wide, H3T11ph is highly enriched at transcription start sites and transcription termination sites, and positively correlated with gene transcript levels. PK6 and SWC4 targeted FLC, MYB73, PRE1, TCP4 and TCP10, depositing H3T11ph at these loci and promoting their transcription, and PK6 occupancy at these loci requires SWC4. Together, our results reveal that nuclear-localized PK6, PK7 and PK8 modulate H3T11ph and plant growth. This study reports that the nucleocytoplasmic shuttling of pyruvate kinase 6 (PK6), PK7 and PK8 mediates phosphorylation of H3 at threonine 11, represses flowering time, and promotes hypocotyl and pedicel elongation in Arabidopsis.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1682-1697"},"PeriodicalIF":15.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair 组蛋白去甲基化酶 JMJ27 在紫外线诱导的 H3K9me2 景观调节过程中发挥作用，并促进光损伤修复

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01814-9

Philippe Johann to Berens, Jackson Peter, Sandrine Koechler, Mathieu Bruggeman, Sébastien Staerck, Jean Molinier

{"title":"The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair","authors":"Philippe Johann to Berens, Jackson Peter, Sandrine Koechler, Mathieu Bruggeman, Sébastien Staerck, Jean Molinier","doi":"10.1038/s41477-024-01814-9","DOIUrl":"10.1038/s41477-024-01814-9","url":null,"abstract":"Plants have evolved sophisticated DNA repair mechanisms to cope with the deleterious effects of ultraviolet (UV)-induced DNA damage. Indeed, DNA repair pathways cooperate with epigenetic-related processes to efficiently maintain genome integrity. However, it remains to be deciphered how photodamages are recognized within different chromatin landscapes, especially in compacted genomic regions such as constitutive heterochromatin. Here we combined cytogenetics and epigenomics to identify that UV-C irradiation induces modulation of the main epigenetic mark found in constitutive heterochromatin, H3K9me2. We demonstrated that the histone demethylase, Jumonji27 (JMJ27), contributes to the UV-induced reduction of H3K9me2 content at chromocentres. In addition, we identified that JMJ27 forms a complex with the photodamage recognition factor, DNA Damage Binding protein 2 (DDB2), and that the fine-tuning of H3K9me2 contents orchestrates DDB2 dynamics on chromatin in response to UV-C exposure. Hence, this study uncovers the unexpected existence of an interplay between photodamage repair and H3K9me2 homeostasis. UV exposure modulates H3K9me2 contents at chromocentres via the histone demethylase JMJ27 which interacts with the DNA damage recognition factor, DDB2, and facilitates photodamage repair. The loading or release of DDB2 at nucleosome sites is optimized by the fine-tuning of H3K9me2.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1698-1709"},"PeriodicalIF":15.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector 植物 PR1 挽救了真菌效应物对质体铁硫蛋白的凝结作用

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01811-y

Jingtao Li, Limei Yang, Shuzhi Ding, Mingming Gao, Yu Yan, Gang Yu, Yaning Zheng, Wenxing Liang

{"title":"Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector","authors":"Jingtao Li, Limei Yang, Shuzhi Ding, Mingming Gao, Yu Yan, Gang Yu, Yaning Zheng, Wenxing Liang","doi":"10.1038/s41477-024-01811-y","DOIUrl":"10.1038/s41477-024-01811-y","url":null,"abstract":"Plant pathogens secrete numerous effectors to promote host infection, but whether any of these toxic proteins undergoes phase separation to manipulate plant defence and how the host copes with this event remain elusive. Here we show that the effector FolSvp2, which is secreted from the fungal pathogen Fusarium oxysporum f. sp. lycopersici (Fol), translocates a tomato iron-sulfur protein (SlISP) from plastids into effector condensates in planta via phase separation. Relocation of SlISP attenuates plant reactive oxygen species production and thus facilitates Fol invasion. The action of FolSvp2 also requires K205 acetylation that prevents ubiquitination-dependent degradation of this protein in both Fol and plant cells. However, tomato has evolved a defence protein, SlPR1. Apoplastic SlPR1 physically interacts with and inhibits FolSvp2 entry into host cells and, consequently, abolishes its deleterious effect. These findings reveal a previously unknown function of PR1 in countering a new mode of effector action. There is a continuous arms race between pathogens and their host plants. Li et al. reveal that PR1 prevents entry of a fungal effector into plant cells from the apoplast that otherwise would quench host defence oxidizing agents via phase separation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1775-1789"},"PeriodicalIF":15.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway Rubisco 为 2-C-methyl-d-erythritol-4-phosphate 途径提供丙酮酸

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01791-z

Sonia E. Evans, Yuan Xu, Matthew E. Bergman, Scott A. Ford, Yingxia Liu, Thomas D. Sharkey, Michael A. Phillips

{"title":"Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway","authors":"Sonia E. Evans, Yuan Xu, Matthew E. Bergman, Scott A. Ford, Yingxia Liu, Thomas D. Sharkey, Michael A. Phillips","doi":"10.1038/s41477-024-01791-z","DOIUrl":"10.1038/s41477-024-01791-z","url":null,"abstract":"RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (Rubisco) produces pyruvate in the chloroplast through β-elimination of the aci-carbanion intermediate1. Here we show that this side reaction supplies pyruvate for isoprenoid, fatty acid and branched-chain amino acid biosynthesis in photosynthetically active tissue. 13C labelling studies of intact Arabidopsis plants demonstrate that the total carbon commitment to pyruvate is too large for phosphoenolpyruvate to serve as a precursor. Low oxygen stimulates Rubisco carboxylase activity and increases pyruvate production and flux through the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which supplies the precursors for plastidic isoprenoid biosynthesis2,3. Metabolome analysis of mutants defective in phosphoenolpyruvate or pyruvate import and biochemical characterization of isolated chloroplasts further support Rubisco as the main source of pyruvate in chloroplasts. Seedlings incorporated exogenous,13C-labelled pyruvate into MEP pathway intermediates, while adult plants did not, underscoring the developmental transition in pyruvate sourcing. Rubisco β-elimination leading to pyruvate constituted 0.7% of the product profile in in vitro assays, which translates to 2% of the total carbon leaving the Calvin–Benson–Bassham cycle. These insights solve the “pyruvate paradox”4, improve the fit of metabolic models for central metabolism and connect the MEP pathway directly to carbon assimilation. As nature’s most important enzyme, Rubisco fixes carbon dioxide in the Calvin–Benson–Bassham cycle. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 10","pages":"1453-1463"},"PeriodicalIF":15.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular basis of plastoquinone reduction in plant cytochrome b6f 植物细胞色素 b6f 中质子醌还原的分子基础

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-03 DOI: 10.1038/s41477-024-01804-x

Sebastian Pintscher, Rafał Pietras, Bohun Mielecki, Mateusz Szwalec, Anna Wójcik-Augustyn, Paulina Indyka, Michał Rawski, Łukasz Koziej, Marcin Jaciuk, Grzegorz Ważny, Sebastian Glatt, Artur Osyczka

{"title":"Molecular basis of plastoquinone reduction in plant cytochrome b6f","authors":"Sebastian Pintscher, Rafał Pietras, Bohun Mielecki, Mateusz Szwalec, Anna Wójcik-Augustyn, Paulina Indyka, Michał Rawski, Łukasz Koziej, Marcin Jaciuk, Grzegorz Ważny, Sebastian Glatt, Artur Osyczka","doi":"10.1038/s41477-024-01804-x","DOIUrl":"10.1038/s41477-024-01804-x","url":null,"abstract":"A multi-subunit enzyme, cytochrome b6f (cytb6f), provides the crucial link between photosystems I and II in the photosynthetic membranes of higher plants, transferring electrons between plastoquinone (PQ) and plastocyanin. The atomic structure of cytb6f is known, but its detailed catalytic mechanism remains elusive. Here we present cryogenic electron microscopy structures of spinach cytb6f at 1.9 Å and 2.2 Å resolution, revealing an unexpected orientation of the substrate PQ in the haem ligand niche that forms the PQ reduction site (Qn). PQ, unlike Qn inhibitors, is not in direct contact with the haem. Instead, a water molecule is coordinated by one of the carbonyl groups of PQ and can act as the immediate proton donor for PQ. In addition, we identify water channels that connect Qn with the aqueous exterior of the enzyme, suggesting that the binding of PQ in Qn displaces water through these channels. The structures confirm large movements of the head domain of the iron–sulfur protein (ISP-HD) towards and away from the plastoquinol oxidation site (Qp) and define the unique position of ISP-HD when a Qp inhibitor (2,5-dibromo-3-methyl-6-isopropylbenzoquinone) is bound. This work identifies key conformational states of cytb6f, highlights fundamental differences between substrates and inhibitors and proposes a quinone–water exchange mechanism. Cryo-EM structures of cytb6f with quinone bound in the Qn site allow the identification of specific proton channels and reveal differences to known inhibitors. Nearby water channels imply a ‘piston-like’ mechanism during catalytic turnover.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1814-1825"},"PeriodicalIF":15.8,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-024-01804-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Megafire smoke exposure jeopardizes tree carbohydrate reserves and yield 特大火灾的烟雾危害树木的碳水化合物储备和产量

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-02 DOI: 10.1038/s41477-024-01819-4

Jessica Orozco, Paula Guzmán-Delgado, Maciej A. Zwieniecki

{"title":"Megafire smoke exposure jeopardizes tree carbohydrate reserves and yield","authors":"Jessica Orozco, Paula Guzmán-Delgado, Maciej A. Zwieniecki","doi":"10.1038/s41477-024-01819-4","DOIUrl":"10.1038/s41477-024-01819-4","url":null,"abstract":"The global incidence of megafires is on the rise, leading to extensive areas being shrouded in dense smoke for prolonged periods, spanning days or weeks1. Here, by integrating long-term regional observations of non-structural carbohydrate content in trees across California’s Central Valley with spatiotemporal satellite data, we present compelling evidence that dense smoke plumes negatively impact carbohydrate stores in three tree species: Prunus dulcis, Pistacia vera and Juglans regia. Our findings show that the presence of smoke causes a significant decrease in total non-structural carbohydrates, with reductions in the accumulation of both soluble sugar and starch reserves. This decline in carbohydrate levels persists through the trees’ dormancy period into the next season’s bloom, culminating in a reduced yield. Our results highlight a previously unrecognized wildfire threat that could affect plant health and ecosystem stability in both agricultural and natural environments. The incidence and severity of megafires are increasing as a consequence of global change. While the impacts of fires on tree physiology and ecosystem functioning are well studied, how smoke affects these processes is less clear. Here Orozco et al. report that wildfire smoke significantly reduces tree carbohydrate reserves and yields, revealing an overlooked consequence of wildfires.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1635-1642"},"PeriodicalIF":15.8,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From RdDM to plant defence 从 RdDM 到植物防御

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-30 DOI: 10.1038/s41477-024-01806-9

Claudia Martinho

引用次数: 0

A novel in vivo genome editing doubled haploid system for Zea mays L. 用于玉米的新型体内基因组编辑加倍单倍体系统

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01795-9

Huaxun Ye, Mei Louden, Jon A. T. Reinders

{"title":"A novel in vivo genome editing doubled haploid system for Zea mays L.","authors":"Huaxun Ye, Mei Louden, Jon A. T. Reinders","doi":"10.1038/s41477-024-01795-9","DOIUrl":"10.1038/s41477-024-01795-9","url":null,"abstract":"Doubled haploid (DH) technologies accelerate maize inbred development. Recently, methods using CRISPR–Cas have created gene-edited maize DH populations, albeit with relatively low editing frequencies. Restoring fertility via haploid chromosome doubling remains a critically important production constraint. Thus, improved editing and chromosome doubling outcomes are needed. Here we obtained maternally derived diploid embryos in vivo by ectopically co-expressing Zea mays BABY BOOM and cyclin D-like gene products within unfertilized egg cells. When combined with gene editing, the in vivo method enables the production of mature seed with a maternally derived, gene-edited diploid embryo without requiring in vitro tissue culture methods nor the use of a chemical chromosome doubling agent. In summary, we report a novel approach for creating gene-edited maize DH populations that we expect can accelerate genetic gain in a scalable, cost-effective manner. Ye et al. use a novel genetic haploid genome doubling method with parthenogenesis and gene editing to produce edited, maternally derived di-haploid progeny. A truncated BABY BOOM peptide confers both parthenogenesis and haploid genome doubling.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 10","pages":"1493-1501"},"PeriodicalIF":15.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide 逆转录转座子驱动的FLC环境调控导致对除草剂的适应性反应

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01807-8

Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana

{"title":"Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide","authors":"Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana","doi":"10.1038/s41477-024-01807-8","DOIUrl":"10.1038/s41477-024-01807-8","url":null,"abstract":"The mobilization of transposable elements is a potent source of mutations. In plants, several stransposable elements respond to external cues, fuelling the hypothesis that natural transposition can create environmentally sensitive alleles for adaptation. Here we report on the detailed characterization of a retrotransposon insertion within the first intron of the Arabidopsis floral-repressor gene FLOWERING LOCUS C (FLC) and the discovery of its role for adaptation. The insertion mutation augments the environmental sensitivity of FLC by affecting the balance between coding and non-coding transcripts in response to stress, thus expediting flowering. This balance is modulated by DNA methylation and orchestrated by IBM2, a factor involved in the processing of intronic heterochromatic sequences. The stress-sensitive allele of FLC has spread across populations subjected to recurrent chemical weeding, and we show that retrotransposon-driven acceleration of the life cycle represents a rapid response to herbicide application. Our work provides a compelling example of a transposable element-driven environmentally sensitive allele that confers an adaptive response in nature. This study reports the discovery of a retrotransposon insertion in the FLC gene conferring an environmentally dependent control mechanism mediated by DNA methylation that drives the adaptation of natural Arabidopsis populations to herbicide exposure.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"10 11","pages":"1672-1681"},"PeriodicalIF":15.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposable elements underlie genetic adaptation 转座元件是遗传适应的基础

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01792-y

Marco Catoni

引用次数: 0