Nature Plants最新文献_第9页

Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity 拟南芥CNGC1和CNGC5的低温电镜结构揭示了门控和钙选择性的分子机制

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-20 DOI: 10.1038/s41477-025-01923-z

Jianping Wang, Bo-Ya Du, Xue Zhang, Xiaomin Qu, Yang Yang, Zhao Yang, Yong-Fei Wang, Peng Zhang

{"title":"Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity","authors":"Jianping Wang, Bo-Ya Du, Xue Zhang, Xiaomin Qu, Yang Yang, Zhao Yang, Yong-Fei Wang, Peng Zhang","doi":"10.1038/s41477-025-01923-z","DOIUrl":"10.1038/s41477-025-01923-z","url":null,"abstract":"Plant cyclic nucleotide-gated channels (CNGCs) belong to the cyclic nucleotide-binding domain (CNBD) channel family, but are phylogenetically classified in a distinct branch. In contrast to their animal counterparts of K+-selective or non-selective cation channels, plant CNGCs mainly mediate Ca2+ influx and are involved in various physiological processes, such as stomatal movements, pollen-tube growth and immune responses. Here, we present the cryo-EM structure and electrophysiological analysis of plant CNGC representatives, Arabidopsis CNGC1 and CNGC5. We found that CNGC1 and CNGC5 contain a unique extracellular domain featuring disulfide bonds that is essential for channel gating via coupling of the voltage-sensing domain with the pore domain. The pore domain selectivity filter possesses a Gln residue at the constriction site that determines the Ca2+ selectivity. Replacement of this Gln with Glu, typically observed in CNBD-type non-selective cation channels, could convert CNGC1 and CNGC5 from Ca2+-selective channels to non-selective cation channels permeable to Ca2+, Na+ or K+. In addition, we found that the CNGC1 and CNGC5 CNBD homology domain contains intrinsic-ligand-like interactions, which may devoid the binding of cyclic nucleotides and lead to gating independent of cAMP or cGMP. This research not only provides a mechanistic understanding of plant CNGCs’ function, but also adds to the comprehensive knowledge of the CNBD channels. Using cryo-EM structures and electrophysiological analysis of Arabidopsis CNGC1 and CNGC5, this study characterizes plant CNGCs as a class of CNBD channels that feature Ca2+ selectivity and are not regulated by cyclic nucleotide monophosphate binding.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 3","pages":"632-642"},"PeriodicalIF":15.8,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota 拟南芥根系微生物群中黄菌共生菌对宿主关联的保守免疫调节和变异

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-19 DOI: 10.1038/s41477-025-01918-w

Jana Ordon, Elke Logemann, Louis-Philippe Maier, Tak Lee, Eik Dahms, Anniek Oosterwijk, Jose Flores-Uribe, Shingo Miyauchi, Lucas Paoli, Sara Christina Stolze, Hirofumi Nakagami, Georg Felix, Ruben Garrido-Oter, Ka-Wai Ma, Paul Schulze-Lefert

{"title":"Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota","authors":"Jana Ordon, Elke Logemann, Louis-Philippe Maier, Tak Lee, Eik Dahms, Anniek Oosterwijk, Jose Flores-Uribe, Shingo Miyauchi, Lucas Paoli, Sara Christina Stolze, Hirofumi Nakagami, Georg Felix, Ruben Garrido-Oter, Ka-Wai Ma, Paul Schulze-Lefert","doi":"10.1038/s41477-025-01918-w","DOIUrl":"10.1038/s41477-025-01918-w","url":null,"abstract":"Suppression of chronic Arabidopsis immune responses is a widespread but typically strain-specific trait across the major bacterial lineages of the plant microbiota. We show by phylogenetic analysis and in planta associations with representative strains that immunomodulation is a highly conserved, ancestral trait across Xanthomonadales, and preceded specialization of some of these bacteria as host-adapted pathogens. Rhodanobacter R179 activates immune responses, yet root transcriptomics suggest this commensal evades host immune perception upon prolonged association. R179 camouflage likely results from combined activities of two transporter complexes (dssAB) and the selective elimination of immunogenic peptides derived from all partners. The ability of R179 to mask itself and other commensals from the plant immune system is consistent with a convergence of distinct root transcriptomes triggered by immunosuppressive or non-suppressive synthetic microbiota upon R179 co-inoculation. Immunomodulation through dssAB provided R179 with a competitive advantage in synthetic communities in the root compartment. We propose that extensive immunomodulation by Xanthomonadales is related to their adaptation to terrestrial habitats and might have contributed to variation in strain-specific root association, which together accounts for their prominent role in plant microbiota establishment. The authors show that immunosuppression is highly conserved in the bacterial order Xanthomonadales. This feature, which preceded their specialization as host-adapted pathogens, probably contributes to their prominence as core members of the plant microbiota.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 3","pages":"612-631"},"PeriodicalIF":15.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01918-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PNET1 is a key regulator of NPC dynamics and cell division PNET1 是 NPC 动态和细胞分裂的关键调控因子

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-17 DOI: 10.1038/s41477-025-01930-0

Katja Graumann, Nadine Field

引用次数: 0

The apoplastic pH is a key determinant in the hypocotyl growth response to auxin dosage and light 外胞体pH是下胚轴生长对生长素剂量和光照响应的关键决定因素

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01910-4

Jiajun Wang, Dan Jin, Zhaoguo Deng, Lidan Zheng, Pengru Guo, Yusi Ji, Zihao Song, Hai Yue Zeng, Toshinori Kinoshita, Zhihua Liao, Haodong Chen, Xing Wang Deng, Ning Wei

{"title":"The apoplastic pH is a key determinant in the hypocotyl growth response to auxin dosage and light","authors":"Jiajun Wang, Dan Jin, Zhaoguo Deng, Lidan Zheng, Pengru Guo, Yusi Ji, Zihao Song, Hai Yue Zeng, Toshinori Kinoshita, Zhihua Liao, Haodong Chen, Xing Wang Deng, Ning Wei","doi":"10.1038/s41477-025-01910-4","DOIUrl":"10.1038/s41477-025-01910-4","url":null,"abstract":"Auxin is a core phytohormone regulating plant elongation growth. While auxin typically promotes hypocotyl elongation, excessive amounts of auxin inhibit elongation. Moreover, auxin usually promotes light-grown, but inhibits dark-grown hypocotyl elongation. How dosage and light condition change the plant’s response to auxin, also known as auxin’s biphasic effect or dual effect, has long been mysterious. Auxin induces cell expansion primarily through apoplastic acidification and the subsequent ‘acid growth’ mechanism. Here we show that this pathway operates for both stimulatory and inhibitory auxin doses and under both dark and light conditions. Regardless of the dosage, more auxin induces more transcripts of SAURs (Small Auxin-Up RNAs), leading to a stronger activation of plasma membrane H+-ATPases (AHAs) and progressive acidification of the apoplast in hypocotyl epidermis. Apoplastic acidification promotes growth but only above a certain pH threshold, below which excessive acidification inhibits elongation. Auxin overdosage-triggered hypocotyl inhibition can be alleviated by suppressing the AHA activity or raising the apoplastic pH. Light-grown hypocotyls exhibit a higher apoplastic pH, which impedes cell elongation and counteracts auxin-induced over-acidification. Auxin and light antagonistically regulate the SAUR-PP2C.D-AHA pathway in the hypocotyl and influence plant elongation growth. Our findings suggest that the biphasic effect of auxin results from the biphasic response of hypocotyl cells to decreasing apoplastic pH. Auxin can promote or inhibit hypocotyl elongation. This biphasic effect has puzzled generations of plant biologists. Wang et al. shows that the decreasing apoplastic pH, stimulated by auxin, underlies the change in hypocotyl growth response.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"279-294"},"PeriodicalIF":15.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01910-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants 哌啶-6-羧酸调节植物体内维生素B6稳态和调节全身免疫

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01906-0

Huazhen Liu, Lakshminarayan M. Iyer, Paul Norris, Ruiying Liu, Keshun Yu, Murray Grant, L. Aravind, Aardra Kachroo, Pradeep Kachroo

{"title":"Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants","authors":"Huazhen Liu, Lakshminarayan M. Iyer, Paul Norris, Ruiying Liu, Keshun Yu, Murray Grant, L. Aravind, Aardra Kachroo, Pradeep Kachroo","doi":"10.1038/s41477-025-01906-0","DOIUrl":"10.1038/s41477-025-01906-0","url":null,"abstract":"Dietary consumption of lysine in humans leads to the biosynthesis of Δ1-piperideine-6-carboxylic acid (P6C), with elevated levels linked to the neurological disorder epilepsy. Here we demonstrate that P6C biosynthesis is also a critical component of lysine catabolism in Arabidopsis thaliana. P6C regulates vitamin B6 homeostasis, and increased P6C levels deplete B6 vitamers, resulting in compromised plant immunity. We further establish a key role for pyridoxal and pyridoxal-5-phosphate biosynthesis in plant immunity. Our analysis indicates that P6C metabolism probably evolved through combining select lysine and proline metabolic enzymes horizontally acquired from diverse bacterial sources at different points during evolution. More generally, certain enzymes from the lysine and proline metabolic pathways were probably recruited in evolution as potential guardians of B6 vitamers and for semialdehyde detoxification. This study identifies the conversion of lysine to Δ1-piperideine-6-carboxylic acid (P6C) via pipecolate oxidase as a conserved pathway in plants and humans. P6C interacts with vitamin B6, affecting its homeostasis. Imbalances in vitamin B6 homeostasis disrupt defence in plants and cause neuropathology in humans.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"263-278"},"PeriodicalIF":15.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of herbicide drift on plant-pollinator interactions 除草剂漂移对植物-传粉者相互作用的影响

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01936-8

Catherine Walker

引用次数: 0

When more becomes too much in acid growth 当在酸性生长中过多时

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01919-9

Pavel Krupař, Matyáš Fendrych

引用次数: 0

Calcium beats for better crops 钙有利于更好的作物

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-13 DOI: 10.1038/s41477-025-01935-9

Guillaume Tena

引用次数: 0

Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis 拟南芥中组蛋白H3赖氨酸4甲基化招募DNA去甲基化酶来加强基因表达

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-11 DOI: 10.1038/s41477-025-01924-y

Ming Wang, Yan He, Zhenhui Zhong, Ashot Papikian, Shuya Wang, Jason Gardiner, Basudev Ghoshal, Suhua Feng, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Steven E. Jacobsen

{"title":"Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis","authors":"Ming Wang, Yan He, Zhenhui Zhong, Ashot Papikian, Shuya Wang, Jason Gardiner, Basudev Ghoshal, Suhua Feng, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Steven E. Jacobsen","doi":"10.1038/s41477-025-01924-y","DOIUrl":"10.1038/s41477-025-01924-y","url":null,"abstract":"Patterning of DNA methylation in eukaryotic genomes is controlled by de novo methylation, maintenance mechanisms and demethylation pathways. In Arabidopsis thaliana, DNA demethylation enzymes are clearly important for shaping methylation patterns, but how they are regulated is poorly understood. Here we show that the targeting of histone H3 lysine four trimethylation (H3K4me3) with the catalytic domain of the SDG2 histone methyltransferase potently erased DNA methylation and gene silencing at FWA and also erased CG DNA methylation in many other regions of the Arabidopsis genome. This methylation erasure was completely blocked in the ros1 dml2 dml3 triple mutant lacking DNA demethylation enzymes, showing that H3K4me3 promotes the active removal of DNA methylation. Conversely, we found that the targeted removal of H3K4me3 increased the efficiency of targeted DNA methylation. These results highlight H3K4me3 as a potent anti-DNA methylation mark and also pave the way for development of more powerful epigenome engineering tools. This study revealed that targeting H3K4me3 via the H3K4 methyltransferase SDG2 activates gene expression and removes DNA methylation by recruiting DNA demethylases. Conversely, the removal of H3K4me3 synergistically enhances targeted DNA methylation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"206-217"},"PeriodicalIF":15.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01924-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATG8ylation of vacuolar membrane protects plants against cell wall damage 液泡膜的atg8酰化保护植物免受细胞壁损伤

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-07 DOI: 10.1038/s41477-025-01907-z

Jose Julian, Peng Gao, Alessia Del Chiaro, Juan Carlos De La Concepcion, Laia Armengot, Marc Somssich, Heloise Duverge, Marion Clavel, Nenad Grujic, Roksolana Kobylinska, Ingo Polivka, Maarten Besten, Tonni Grube Andersen, Christian Dank, Barbara Korbei, Andreas Bachmair, Nuria S. Coll, Elena A. Minina, Joris Sprakel, Yasin Dagdas

{"title":"ATG8ylation of vacuolar membrane protects plants against cell wall damage","authors":"Jose Julian, Peng Gao, Alessia Del Chiaro, Juan Carlos De La Concepcion, Laia Armengot, Marc Somssich, Heloise Duverge, Marion Clavel, Nenad Grujic, Roksolana Kobylinska, Ingo Polivka, Maarten Besten, Tonni Grube Andersen, Christian Dank, Barbara Korbei, Andreas Bachmair, Nuria S. Coll, Elena A. Minina, Joris Sprakel, Yasin Dagdas","doi":"10.1038/s41477-025-01907-z","DOIUrl":"10.1038/s41477-025-01907-z","url":null,"abstract":"Vacuoles are essential for cellular metabolism and growth and the maintenance of internal turgor pressure. They sequester lytic enzymes, ions and secondary metabolites that, if leaked into the cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved vacuolar quality control pathway that is activated upon cell wall damage in a turgor-pressure-dependent manner. Cell wall perturbations induce a distinct modification—ATG8ylation—on the vacuolar membrane (tonoplast) that is regulated by the V-ATPase and ATG8 conjugation machinery. Genetic disruption of tonoplast ATG8ylation impairs vacuolar integrity, leading to cell death. Together, our findings reveal a homeostatic pathway that preserves vacuolar integrity upon cell wall damage. ATG8ylation of the tonoplast, triggered by cell wall damage, acts as a vital vacuolar quality control mechanism that safeguards vacuolar integrity and ensures cell survival under stress.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"321-339"},"PeriodicalIF":15.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01907-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0