Nature Plants最新文献

{"title":"Making a new epidermis after abscission","authors":"Aurore Guerault, Christiane Nawrath","doi":"10.1038/s41477-025-01951-9","DOIUrl":"https://doi.org/10.1038/s41477-025-01951-9","url":null,"abstract":"After abscission, non-epidermal residuum cells specify de novo into epidermal cells, which synthesize a cuticle. New work reveals that MYB74 primarily mediates this transdifferentiation process, which occurs in three stages and balances plant protection and growth in an effective manner.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"121 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unequal signalling and biosynthesis in daughter cells","authors":"Raphael Trösch","doi":"10.1038/s41477-025-01992-0","DOIUrl":"https://doi.org/10.1038/s41477-025-01992-0","url":null,"abstract":"<p>The stem cells in the root apical meristem divide to give rise to transit-amplifying cells, which eventually exit from the cell cycle for elongation and differentiation. Low levels of brassinosteroids are required for cell cycle progression in the meristem, whereas high levels promote cell cycle exit in the elongation zone. Brassinosteroid signalling in dividing cells is therefore probably dynamically regulated. OCTOPUS (OPS) and OCTOPUS-LIKE (OPL) proteins are brassinosteroid-responsive regulators of phloem development that are polarly localized to the apical plasma membrane, but whether they are involved in dynamic regulation of brassinosteroid signalling in dividing cells was unknown.</p><p>The researchers first obtained single-cell RNA-sequencing datasets to generate a cell cycle reference for root tissue. They found that brassinosteroid-responsive genes are enriched in a module that corresponds to the G1 phase. This was confirmed by live-cell imaging, which showed that nuclear accumulation of the brassinosteroid-responsive transcription factor BRASSINAZOLE RESISTANT 1 (BZR1) peaked in the G1 phase and declined during mitosis. The brassinosteroid biosynthesis gene <i>DWARF 4</i> (<i>DWF4</i>) displays a similar cell cycle expression pattern to that of BZR1, and levels were at their minimum during mitosis. Interestingly, the post-mitotic recovery of BZR1 nuclear accumulation is more pronounced in the upper daughter cell, whereas the recovery of <i>DWF4</i> initially occurs in the lower daughter cell. The unequal recovery of BZR1 in the daughter cells did not depend on gene expression or protein degradation, but was rather due to phosphorylation-dependent displacement from the nucleus mediated by <i>Arabidopsis thaliana</i> SHAGGY-related protein kinases (ATSKs). Interestingly, OPL2 interacts with ATSK32; overexpression of OPL2 reduces the nuclear localization and total level of ATSK32. As OPS and OPL proteins are passed on to the upper daughter cell owing to their polar localization in the mother cell, this could explain the preferential post-mitotic nuclear accumulation of BZR1 in the upper daughter cell. Conversely, OPS or OPL mediated nuclear BZR1 accumulation is delayed in the lower daughter cell, which allows it to escape the negative feedback loop and express brassinosteroid biosynthesis genes such as <i>DWF4</i>. This mechanism establishes a balance between brassinosteroid signalling and biosynthesis that optimizes root growth, as was also demonstrated by computational modelling.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"223 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB74 transcription factor guides de novo specification of epidermal cells in the abscission zone of Arabidopsis","authors":"Xiaohong Wen, Chan Woong Lee, Seonghwan Kim, Jae-Ung Hwang, Yoon Ha Choi, Soon-Ki Han, Eunmin Lee, Taek-Han Yoon, Dong Gon Cha, Seulbee Lee, Heejeong Son, Jiwon Son, Su Hyun Jung, Jiyoun Lee, Heejin Lim, Huize Chen, Jong Kyoung Kim, June M. Kwak","doi":"10.1038/s41477-025-01976-0","DOIUrl":"https://doi.org/10.1038/s41477-025-01976-0","url":null,"abstract":"<p>The waxy cuticle layer is crucial for plant defence, growth and survival, and is produced by epidermal cells, which were thought to be specified only during embryogenesis. New surface cells are exposed during abscission, by which leaves, fruits, flowers and seeds are shed. Recent work has shown that nonepidermal residuum cells (RECs) can accumulate a protective cuticle layer after abscission, implying the potential de novo specification of epidermal cells by transdifferentiation. However, it remains unknown how this process occurs and what advantage this mechanism may offer over the other surface protection alternative, the wound healing pathways. Here we followed this transdifferentiation process with single-cell RNA sequencing analysis of RECs, showing that nonepidermal RECs transdifferentiate into epidermal cells through three distinct stages. During this vulnerable process, which involves a transient period when the protective layer is not yet formed, stress genes that protect the plant from environmental exposure are expressed before epidermis formation, ultimately facilitating cuticle development. We identify a central role for the transcription factor MYB74 in directing the transdifferentiation. In contrast to alternative protective mechanisms, our results suggest that de novo epidermal specification supports the subsequent growth of fruit at the abscission site. Altogether, we reveal a developmental programme by which plants use a transdifferentiation pathway to protect the plant while promoting growth.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"21 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uniform regulation of stomatal closure across temperate tree species to sustain nocturnal turgor and growth","authors":"Richard L. Peters, Matthias Arend, Cedric Zahnd, Günter Hoch, Stefan K. Arndt, Lucas A. Cernusak, Rafael Poyatos, Tobias Zhorzel, Ansgar Kahmen","doi":"10.1038/s41477-025-01957-3","DOIUrl":"https://doi.org/10.1038/s41477-025-01957-3","url":null,"abstract":"<p>Water loss and carbon gain are balanced by stomatal control¹, a trade-off that has allowed trees to survive and thrive under fluctuating environmental conditions^2,3,4. During periods of lower water availability, stomatal closure prevents excess water loss⁵. Various strategies of stomatal control have been found among tree species^6,7, but the trigger for this behaviour remains elusive. We found a uniform pre-dawn water potential threshold (−1.2 MPa) for stomatal closure across species, which coincided with stem-growth cessation. Meanwhile, midday water potentials at stomatal closure were more variable across species and stomatal control did not follow species-specific thresholds of hydraulic failure, a commonly adopted theory in plant biology^8,9,10, and often used in predictive water-use modelling^11,12. This indicates that nocturnal rehydration, rather than daytime hydraulic safety is an optimization priority for stomatal closure in trees¹³. We suggest that these processes are critical for forecasting the global carbon cycle dynamics.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"8 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future crop breeding needs to consider future soils","authors":"Sajjad Raza, Bipin K. Pandey, Malcolm J. Hawkesford, Simon Griffiths, Malcolm J. Bennett, Sacha J. Mooney","doi":"10.1038/s41477-025-01977-z","DOIUrl":"https://doi.org/10.1038/s41477-025-01977-z","url":null,"abstract":"Modern crop breeding and seed certification agencies ignore the known spatial heterogeneity of soils and develop cultivars to thrive in a ‘one-size-fits-all’ soil environment. Neglecting the evolving dynamics of soils substantially undermines the capacity of new genotypes to deliver optimal yield and stress resilience, and requires urgent consideration in future plant breeding programmes.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"58 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143758391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental innovation of inferior ovaries and flower sex orchestrated by KNOX1 in cucurbits","authors":"Zhaonian Dong, Xiaolin Liu, Xing Guo, Xun Liu, Bowen Wang, Wenwen Shao, Caihuan Tian, Yingying Zheng, Qiong Yu, Liyuan Zhong, Jinjing Sun, Shengkang Li, Tongxu Xin, Bohan Zhang, Tao Yang, Haorong Lu, Jocelyn K. C. Rose, William J. Lucas, Xun Xu, Sanwen Huang, Huan Liu, Xueyong Yang","doi":"10.1038/s41477-025-01950-w","DOIUrl":"https://doi.org/10.1038/s41477-025-01950-w","url":null,"abstract":"<p>In flowering plants, inferior ovaries are key morphological innovations that evolved multiple times from superior ovaries to protect female parts of the flower. However, the developmental mechanisms underlying inferior ovary formation remain largely unknown. Comparative spatial transcriptome mapping and cell lineage reconstructions in developing floral buds of cucumber and tomato, which have inferior and superior ovaries, respectively, revealed that inferior ovaries develop from accelerated receptacle growth resulting from the continuous activity of meristematic stems cells at the base of the cucumber floral organs. Genetic knockout of a receptacle-specific KNOX1 transcription factor in cucumber caused arrest in receptacle growth and yielded bisexual flowers with superior ovaries similar to those of tomato. Here we provide developmental and mechanistic insights into inferior ovary formation and sex determination in cucurbits.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"75 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The future of genome editing in plants","authors":"Larry Gilbertson, Holger Puchta, R. Keith Slotkin","doi":"10.1038/s41477-025-01956-4","DOIUrl":"https://doi.org/10.1038/s41477-025-01956-4","url":null,"abstract":"<p>The future of genome editing in plants differs from how it is used today. For both research and product development, we need to think beyond the creation of simple single-nucleotide polymorphisms and short deletions in genes. We believe that the future of genome editing in plants involves mimicking the natural evolutionary processes that have shaped plant genomes and been the target of artificial selection during crop domestication and improvement. This includes programming large structural variations (insertions, duplications, deletions, inversions and translocations) and controlling plant recombination and endogenous transposable elements that naturally reshape plant genomes. The key is that genome editing will be used to reshape plant genomes in a manner that could have happened naturally, but now these changes can be directed rapidly in the laboratory.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"58 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143745312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-canonical plant metabolism","authors":"Lee J. Sweetlove, R. George Ratcliffe, Alisdair R. Fernie","doi":"10.1038/s41477-025-01965-3","DOIUrl":"https://doi.org/10.1038/s41477-025-01965-3","url":null,"abstract":"<p>Metabolism is essential for plant growth and has become a major target for crop improvement by enhancing nutrient use efficiency. Metabolic engineering is also the basis for producing high-value plant products such as pharmaceuticals, biofuels and industrial biochemicals. An inherent problem for such engineering endeavours is the tendency to view metabolism as a series of distinct metabolic pathways—glycolysis, the tricarboxylic acid cycle, the Calvin–Benson cycle and so on. While these canonical pathways may represent a dominant or frequently occurring flux mode, systematic analyses of metabolism via computational modelling have emphasized the inherent flexibility of the metabolic network to carry flux distributions that are distinct from the canonical pathways. Recent experimental estimates of metabolic network fluxes using ¹³C-labelling approaches have revealed numerous instances in which non-canonical pathways occur under different conditions and in different tissues. In this Review, we bring these non-canonical pathways to the fore, summarizing the evidence for their occurrence and the context in which they operate. We also emphasize the importance of non-canonical pathways for metabolic engineering. We argue that the introduction of a high-flux pathway to a desired metabolic product will, by necessity, require non-canonical supporting fluxes in central metabolism to provide the necessary carbon skeletons, energy and reducing power. We illustrate this using the overproduction of isoprenoids and fatty acids as case studies.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"31 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H3K36 methylation stamps transcription resistive to preserve development in plants","authors":"Yao Yao, Jincong Zhou, Jiacheng Wang, Xue Lei, Anjie Jiang, Qianwen Sun","doi":"10.1038/s41477-025-01962-6","DOIUrl":"https://doi.org/10.1038/s41477-025-01962-6","url":null,"abstract":"<p>Eukaryotic euchromatin is the less-compact chromatin and is modified by many histone modifications such as H3 lysine 36 methylation (H3K36me). Here we report a new chromatin state, ‘transcription resistive’, which is differentiated from activation and silencing. Transcription resistive is stamped by H3K36me with almost undetectable transcription activity but open-chromatin state, and occupies most documented plant essential genes. Mutating SDG8, previously known as the major H3K36 methyltransferase in <i>Arabidopsis</i>, surprisingly elevates 78.7% of H3K36me3-marked resistive loci, which accounts for 39.4% of the coding genome. Genetically, SDG8 prevents H3K36me activity of SDG4 at short and intronless genes to secure plant fertility, while it collaborates with other H3K36me methyltransferases on long and intron-rich genes. Together, our results reveal that SDG8 is the primary sensor that suppresses excessive H3K36me, and uncovered that ‘transcription resistive’ is a conserved H3K36me-stamped novel transcription state in plants, highlighting the regulatory diversities and biological significance of H3K36 methylation in eukaryotes.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"49 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Horizontal gene transfer of cold shock protein genes boosted wheat adaptation and expansion","authors":"","doi":"10.1038/s41477-025-01985-z","DOIUrl":"https://doi.org/10.1038/s41477-025-01985-z","url":null,"abstract":"CSP-H genes (encoding cold shock proteins) were horizontally transferred from bacteria to Triticeae and improved wheat adaptation by enhancing its tolerance to various abiotic stresses. Because these genes are integrated into the recipient genome and have been positively selected for thousands of years, they provide great potential for modern transgenic engineering and synthetic biology.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"172 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143736962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}