Nature Plants最新文献

Regulation of alternative splicing by CBF-mediated protein condensation in plant response to cold stress

IF 18 1区生物学

Nature Plants Pub Date : 2025-03-05 DOI: 10.1038/s41477-025-01933-x

Diyi Fu, Yue Song, Shifeng Wu, Yue Peng, Yuhang Ming, Zhuoyang Li, Xiaoyan Zhang, Wen Song, Zhen Su, Zhizhong Gong, Shuhua Yang, Yiting Shi

{"title":"Regulation of alternative splicing by CBF-mediated protein condensation in plant response to cold stress","authors":"Diyi Fu, Yue Song, Shifeng Wu, Yue Peng, Yuhang Ming, Zhuoyang Li, Xiaoyan Zhang, Wen Song, Zhen Su, Zhizhong Gong, Shuhua Yang, Yiting Shi","doi":"10.1038/s41477-025-01933-x","DOIUrl":"https://doi.org/10.1038/s41477-025-01933-x","url":null,"abstract":"Cold acclimation is critical for the survival of plants in temperate regions under low temperatures, and C-REPEAT BINDING FACTORs (CBFs) are well established as key transcriptional factors that regulate this adaptive process by controlling the expression of cold-responsive genes. Here we demonstrate that CBFs are involved in modulating alternative splicing during cold acclimation through their interaction with subunits of the spliceosome complex. Under cold stress, CBF proteins accumulate and directly interact with SKI-INTERACTING PROTEIN (SKIP), a key component of the spliceosome, which positively regulates acquired freezing tolerance. This interaction facilitates the formation of SKIP nuclear condensates, which enhances the association between SKIP and specific cold-responsive transcripts, thereby increasing their splicing efficiency. Our findings uncover a regulatory role of CBFs in alternative splicing and highlight their pivotal involvement in the full development of cold acclimation, bridging transcriptional and post-transcriptional regulatory mechanisms.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"131 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural basis for the activation of plant bunyavirus replication machinery and its dual-targeted inhibition by ribavirin

IF 18 1区生物学

Nature Plants Pub Date : 2025-03-05 DOI: 10.1038/s41477-025-01940-y

Jia Li, Lei Cao, Yaqian Zhao, Jinghan Shen, Lei Wang, Mingfeng Feng, Min Zhu, Yonghao Ye, Richard Kormelink, Xiaorong Tao, Xiangxi Wang

{"title":"Structural basis for the activation of plant bunyavirus replication machinery and its dual-targeted inhibition by ribavirin","authors":"Jia Li, Lei Cao, Yaqian Zhao, Jinghan Shen, Lei Wang, Mingfeng Feng, Min Zhu, Yonghao Ye, Richard Kormelink, Xiaorong Tao, Xiangxi Wang","doi":"10.1038/s41477-025-01940-y","DOIUrl":"https://doi.org/10.1038/s41477-025-01940-y","url":null,"abstract":"Despite the discovery of plant viruses as a new class of pathogens over a century ago, the structure of plant virus replication machinery and antiviral pesticide remains lacking. Here we report five cryogenic electron microscopy structures of a ~330-kDa RNA-dependent RNA polymerase (RdRp) from a devastating plant bunyavirus, tomato spotted wilt orthotospovirus (TSWV), including the apo, viral-RNA-bound, base analogue ribavirin-bound and ribavirin-triphosphate-bound states. They reveal that a flexible loop of RdRp’s motif F functions as ‘sensor’ to perceive viral RNA and further acts as an ‘adaptor’ to promote the formation of a complete catalytic centre. A ten-base RNA ‘hook’ structure is sufficient to trigger major conformational changes and activate RdRp. Chemical screening showed that ribavirin is effective against TSWV, and structural data revealed that ribavirin disrupts both hook-binding and catalytic core formation, locking polymerase in its inactive state. This work provides structural insights into the mechanisms of plant bunyavirus RdRp activation and its dual-targeted site inhibition, facilitating the development of pesticides against plant viruses.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"30 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize

IF 18 1区生物学

Nature Plants Pub Date : 2025-03-05 DOI: 10.1038/s41477-025-01934-w

Guannan Jia, Guojingwei Chen, Zhaoheng Zhang, Chenghua Tian, Yaping Wang, Jie Luo, Kaina Zhang, Xiaoyun Zhao, Xiaoming Zhao, Zhen Li, Linfeng Sun, Wenqiang Yang, Yan Guo, Jiří Friml, Zhizhong Gong, Jing Zhang

{"title":"Ferredoxin-mediated mechanism for efficient nitrogen utilization in maize","authors":"Guannan Jia, Guojingwei Chen, Zhaoheng Zhang, Chenghua Tian, Yaping Wang, Jie Luo, Kaina Zhang, Xiaoyun Zhao, Xiaoming Zhao, Zhen Li, Linfeng Sun, Wenqiang Yang, Yan Guo, Jiří Friml, Zhizhong Gong, Jing Zhang","doi":"10.1038/s41477-025-01934-w","DOIUrl":"https://doi.org/10.1038/s41477-025-01934-w","url":null,"abstract":"Nitrogen (N) is an essential macronutrient for plant development and, ultimately, yield. Identifying the genetic components and mechanisms underlying N use efficiency in maize (Zea mays L.) is thus of great importance. Nitrate (NO3−) is the preferred inorganic N source in maize. Here we performed a genome-wide association study of shoot NO3− accumulation in maize seedlings grown under low-NO3− conditions, identifying the ferredoxin family gene ZmFd4 as a major contributor to this trait. ZmFd4 interacts and co-localizes with nitrite reductases (ZmNiRs) in chloroplasts to promote their enzymatic activity. Furthermore, ZmFd4 forms a high-affinity heterodimer with its closest paralogue, ZmFd9, in a NO3−-sensitive manner. Although ZmFd4 exerts similar biochemical functions as ZmFd9, ZmFd4 and ZmFd9 interaction limits their ability to associate with ZmNiRs and stimulate their activity. Knockout lines for ZmFd4 with decreased NO3− contents exhibit more efficient NO3− assimilation, and field experiments show consistently improved N utilization and grain yield under N-deficient conditions. Our work thus provides molecular and mechanistic insights into the natural variation in N utilization, instrumental for genetic improvement of yield in maize and, potentially, in other crops.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"23 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Author Correction: MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules.

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-03-03 DOI: 10.1038/s41477-025-01959-1

Xiujuan Yang, Gang Li, Jin Shi, Laura G Wilkinson, Matthew K Aubert, Kelly Houston, Neil J Shirley, Hengbin Gao, Ryan Lister, Lucia Colombo, Matthew R Tucker

引用次数: 0

Author Correction: Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants.

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-03-03 DOI: 10.1038/s41477-025-01960-8

Huazhen Liu, Lakshminarayan M Iyer, Paul Norris, Ruiying Liu, Keshun Yu, Murray Grant, L Aravind, Aardra Kachroo, Pradeep Kachroo

引用次数: 0

MicroRNA control of stem cell reconstitution and growth in root regeneration

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-25 DOI: 10.1038/s41477-025-01922-0

J. L. Baulies, R. E. Rodríguez, F. E. Lazzara, D. Liebsch, X. Zhao, J. Zeng, L. Bald, C. Schommer, J. U. Lohmann, J. F. Palatnik

{"title":"MicroRNA control of stem cell reconstitution and growth in root regeneration","authors":"J. L. Baulies, R. E. Rodríguez, F. E. Lazzara, D. Liebsch, X. Zhao, J. Zeng, L. Bald, C. Schommer, J. U. Lohmann, J. F. Palatnik","doi":"10.1038/s41477-025-01922-0","DOIUrl":"https://doi.org/10.1038/s41477-025-01922-0","url":null,"abstract":"Plants display a remarkable regeneration capacity, which allows them to replace damaged or lost cells, tissues and organs, and thus recover from a broad spectrum of injuries1,2. Even lost stem cells can be regenerated from non-stem cells after competence acquisition, highlighting the enormous plasticity of plant cells. However, the molecular mechanisms underlying this process are still poorly understood. In the root, the highly conserved microRNA miR396 and its targets, the GROWTH-REGULATING FACTORs (GRFs), control the transition from stem cells to proliferative cells. miR396 promotes stem cell activity by repressing and excluding the GRFs from the stem cell area. In turn, the GRFs promote cell division in the proliferation zone3. Here we show that the miR396–GRF regulatory module guides stem cell reconstitution after root tip excision, playing a dual role: while miR396 promotes competence, the GRFs control regeneration speed. Moreover, plants with ectopic miR396 expression have defined stem cell niches before the excision but do not reconstitute them afterwards, remaining in an open state despite continuing to grow. We propose that this phenomenon is caused by dispersed stem cell activity, which supports growth after root tip excision without reconstituting the organized and spatially restricted stem cell niche typical of Arabidopsis roots.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"3 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143486380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRUTUS links iron with legume–rhizobia symbiosis

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-25 DOI: 10.1038/s41477-025-01939-5

Penelope M. C. Smith, Manuel González-Guerrero

引用次数: 0

A microRNA defines root regeneration

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-25 DOI: 10.1038/s41477-025-01928-8

Ziyao Hu, Huibin Han, Guodong Wang

引用次数: 0

MADS31 coordinates germline development in cereal ovules

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-25 DOI: 10.1038/s41477-025-01916-y

引用次数: 0

MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-25 DOI: 10.1038/s41477-025-01915-z

Xiujuan Yang, Gang Li, Jin Shi, Laura G. Wilkinson, Matthew K. Aubert, Kelly Houston, Neil J. Shirley, Hengbin Gao, Ryan A. Lister, Lucia Colombo, Matthew R. Tucker

{"title":"MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules","authors":"Xiujuan Yang, Gang Li, Jin Shi, Laura G. Wilkinson, Matthew K. Aubert, Kelly Houston, Neil J. Shirley, Hengbin Gao, Ryan A. Lister, Lucia Colombo, Matthew R. Tucker","doi":"10.1038/s41477-025-01915-z","DOIUrl":"https://doi.org/10.1038/s41477-025-01915-z","url":null,"abstract":"The female germline of flowering plants develops within a niche of sporophytic (somatic) ovule cells, also referred to as the nucellus. How niche cells maintain their own somatic developmental programme, yet support the development of adjoining germline cells, remains largely unknown. Here we report that MADS31, a conserved MADS-box transcription factor from the B-sister subclass, is a potent regulator of niche cell identity. In barley, MADS31 is preferentially expressed in nucellar cells directly adjoining the germline, and loss-of-function mads31 mutants exhibit deformed and disorganized nucellar cells, leading to impaired germline development and partial female sterility. Remarkably similar phenotypes are observed in mads31 mutants in wheat, suggesting functional conservation within the Triticeae tribe. Molecular assays indicate that MADS31 encodes a potent transcriptional repressor, targeting genes in the ovule that are normally active in the seed. One prominent target of MADS31 is NRPD4b, a seed-expressed component of RNA polymerase IV/V that is involved in epigenetic regulation. NRPD4b is directly repressed by MADS31 in vivo and is derepressed in mads31 ovules, while overexpression of NRPD4b recapitulates the mads31 ovule phenotype. Thus, repression of NRPD4b by MADS31 is required to maintain ovule niche functionality. Our findings reveal a new mechanism by which somatic ovule tissues maintain their identity and support germline development before transitioning to the post-fertilization programme.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"14 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143485899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0