Nature Plants最新文献_第2页

Co-option and neofunctionalization of stomatal executors for defence against herbivores in Brassicales 芸苔属植物气孔执行器的协同选择和新功能化以抵御食草动物的侵害

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-24 DOI: 10.1038/s41477-025-01921-1

Makoto Shirakawa, Tomoki Oguro, Shigeo S. Sugano, Shohei Yamaoka, Mayu Sagara, Mai Tanida, Kyoko Sunuma, Takuya Iwami, Tatsuyoshi Nakanishi, Keita Horiuchi, Kie Kumaishi, Soma Yoshida, Mutsumi Watanabe, Takayuki Tohge, Takamasa Suzuki, Yasunori Ichihashi, Atsushi Takemiya, Nobutoshi Yamaguchi, Takayuki Kohchi, Toshiro Ito

{"title":"Co-option and neofunctionalization of stomatal executors for defence against herbivores in Brassicales","authors":"Makoto Shirakawa, Tomoki Oguro, Shigeo S. Sugano, Shohei Yamaoka, Mayu Sagara, Mai Tanida, Kyoko Sunuma, Takuya Iwami, Tatsuyoshi Nakanishi, Keita Horiuchi, Kie Kumaishi, Soma Yoshida, Mutsumi Watanabe, Takayuki Tohge, Takamasa Suzuki, Yasunori Ichihashi, Atsushi Takemiya, Nobutoshi Yamaguchi, Takayuki Kohchi, Toshiro Ito","doi":"10.1038/s41477-025-01921-1","DOIUrl":"https://doi.org/10.1038/s41477-025-01921-1","url":null,"abstract":"Co-option of gene regulatory networks leads to the acquisition of new cell types and tissues. Stomata, valves formed by guard cells (GCs), are present in most land plants and regulate CO2 exchange. The transcription factor (TF) FAMA globally regulates GC differentiation. In the Brassicales, FAMA also promotes the development of idioblast myrosin cells (MCs), another type of specialized cell along the vasculature essential for Brassicales-specific chemical defences. Here we show that in Arabidopsis thaliana, FAMA directly induces the TF gene WASABI MAKER (WSB), which triggers MC differentiation. WSB and STOMATAL CARPENTER 1 (SCAP1, a stomatal lineage-specific direct FAMA target), synergistically promote GC differentiation. wsb mutants lacked MCs and the wsb scap1 double mutant lacked normal GCs. Evolutionary analyses revealed that WSB is conserved across stomatous angiosperms. We propose that the conserved and reduced transcriptional FAMA–WSB module was co-opted before evolving to induce MC differentiation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"19 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143477696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finding factors that enforce the multifaceted functions of FAMA

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-24 DOI: 10.1038/s41477-024-01890-x

Margot E. Smit

引用次数: 0

Loving the alien

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-21 DOI: 10.1038/s41477-025-01941-x

引用次数: 0

Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-20 DOI: 10.1038/s41477-025-01923-z

Jianping Wang, Bo-Ya Du, Xue Zhang, Xiaomin Qu, Yang Yang, Zhao Yang, Yong-Fei Wang, Peng Zhang

{"title":"Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity","authors":"Jianping Wang, Bo-Ya Du, Xue Zhang, Xiaomin Qu, Yang Yang, Zhao Yang, Yong-Fei Wang, Peng Zhang","doi":"10.1038/s41477-025-01923-z","DOIUrl":"https://doi.org/10.1038/s41477-025-01923-z","url":null,"abstract":"Plant cyclic nucleotide-gated channels (CNGCs) belong to the cyclic nucleotide-binding domain (CNBD) channel family, but are phylogenetically classified in a distinct branch. In contrast to their animal counterparts of K+-selective or non-selective cation channels, plant CNGCs mainly mediate Ca2+ influx and are involved in various physiological processes, such as stomatal movements, pollen-tube growth and immune responses. Here, we present the cryo-EM structure and electrophysiological analysis of plant CNGC representatives, Arabidopsis CNGC1 and CNGC5. We found that CNGC1 and CNGC5 contain a unique extracellular domain featuring disulfide bonds that is essential for channel gating via coupling of the voltage-sensing domain with the pore domain. The pore domain selectivity filter possesses a Gln residue at the constriction site that determines the Ca2+ selectivity. Replacement of this Gln with Glu, typically observed in CNBD-type non-selective cation channels, could convert CNGC1 and CNGC5 from Ca2+-selective channels to non-selective cation channels permeable to Ca2+, Na+ or K+. In addition, we found that the CNGC1 and CNGC5 CNBD homology domain contains intrinsic-ligand-like interactions, which may devoid the binding of cyclic nucleotides and lead to gating independent of cAMP or cGMP. This research not only provides a mechanistic understanding of plant CNGCs’ function, but also adds to the comprehensive knowledge of the CNBD channels.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"23 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-19 DOI: 10.1038/s41477-025-01918-w

Jana Ordon, Elke Logemann, Louis-Philippe Maier, Tak Lee, Eik Dahms, Anniek Oosterwijk, Jose Flores-Uribe, Shingo Miyauchi, Lucas Paoli, Sara Christina Stolze, Hirofumi Nakagami, Georg Felix, Ruben Garrido-Oter, Ka-Wai Ma, Paul Schulze-Lefert

{"title":"Conserved immunomodulation and variation in host association by Xanthomonadales commensals in Arabidopsis root microbiota","authors":"Jana Ordon, Elke Logemann, Louis-Philippe Maier, Tak Lee, Eik Dahms, Anniek Oosterwijk, Jose Flores-Uribe, Shingo Miyauchi, Lucas Paoli, Sara Christina Stolze, Hirofumi Nakagami, Georg Felix, Ruben Garrido-Oter, Ka-Wai Ma, Paul Schulze-Lefert","doi":"10.1038/s41477-025-01918-w","DOIUrl":"https://doi.org/10.1038/s41477-025-01918-w","url":null,"abstract":"Suppression of chronic Arabidopsis immune responses is a widespread but typically strain-specific trait across the major bacterial lineages of the plant microbiota. We show by phylogenetic analysis and in planta associations with representative strains that immunomodulation is a highly conserved, ancestral trait across Xanthomonadales, and preceded specialization of some of these bacteria as host-adapted pathogens. Rhodanobacter R179 activates immune responses, yet root transcriptomics suggest this commensal evades host immune perception upon prolonged association. R179 camouflage likely results from combined activities of two transporter complexes (dssAB) and the selective elimination of immunogenic peptides derived from all partners. The ability of R179 to mask itself and other commensals from the plant immune system is consistent with a convergence of distinct root transcriptomes triggered by immunosuppressive or non-suppressive synthetic microbiota upon R179 co-inoculation. Immunomodulation through dssAB provided R179 with a competitive advantage in synthetic communities in the root compartment. We propose that extensive immunomodulation by Xanthomonadales is related to their adaptation to terrestrial habitats and might have contributed to variation in strain-specific root association, which together accounts for their prominent role in plant microbiota establishment.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"14 48 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143443315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PNET1 is a key regulator of NPC dynamics and cell division PNET1 是 NPC 动态和细胞分裂的关键调控因子

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-17 DOI: 10.1038/s41477-025-01930-0

Katja Graumann, Nadine Field

引用次数: 0

The apoplastic pH is a key determinant in the hypocotyl growth response to auxin dosage and light

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01910-4

Jiajun Wang, Dan Jin, Zhaoguo Deng, Lidan Zheng, Pengru Guo, Yusi Ji, Zihao Song, Hai Yue Zeng, Toshinori Kinoshita, Zhihua Liao, Haodong Chen, Xing Wang Deng, Ning Wei

{"title":"The apoplastic pH is a key determinant in the hypocotyl growth response to auxin dosage and light","authors":"Jiajun Wang, Dan Jin, Zhaoguo Deng, Lidan Zheng, Pengru Guo, Yusi Ji, Zihao Song, Hai Yue Zeng, Toshinori Kinoshita, Zhihua Liao, Haodong Chen, Xing Wang Deng, Ning Wei","doi":"10.1038/s41477-025-01910-4","DOIUrl":"10.1038/s41477-025-01910-4","url":null,"abstract":"Auxin is a core phytohormone regulating plant elongation growth. While auxin typically promotes hypocotyl elongation, excessive amounts of auxin inhibit elongation. Moreover, auxin usually promotes light-grown, but inhibits dark-grown hypocotyl elongation. How dosage and light condition change the plant’s response to auxin, also known as auxin’s biphasic effect or dual effect, has long been mysterious. Auxin induces cell expansion primarily through apoplastic acidification and the subsequent ‘acid growth’ mechanism. Here we show that this pathway operates for both stimulatory and inhibitory auxin doses and under both dark and light conditions. Regardless of the dosage, more auxin induces more transcripts of SAURs (Small Auxin-Up RNAs), leading to a stronger activation of plasma membrane H+-ATPases (AHAs) and progressive acidification of the apoplast in hypocotyl epidermis. Apoplastic acidification promotes growth but only above a certain pH threshold, below which excessive acidification inhibits elongation. Auxin overdosage-triggered hypocotyl inhibition can be alleviated by suppressing the AHA activity or raising the apoplastic pH. Light-grown hypocotyls exhibit a higher apoplastic pH, which impedes cell elongation and counteracts auxin-induced over-acidification. Auxin and light antagonistically regulate the SAUR-PP2C.D-AHA pathway in the hypocotyl and influence plant elongation growth. Our findings suggest that the biphasic effect of auxin results from the biphasic response of hypocotyl cells to decreasing apoplastic pH. Auxin can promote or inhibit hypocotyl elongation. This biphasic effect has puzzled generations of plant biologists. Wang et al. shows that the decreasing apoplastic pH, stimulated by auxin, underlies the change in hypocotyl growth response.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"279-294"},"PeriodicalIF":15.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01910-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01906-0

Huazhen Liu, Lakshminarayan M. Iyer, Paul Norris, Ruiying Liu, Keshun Yu, Murray Grant, L. Aravind, Aardra Kachroo, Pradeep Kachroo

{"title":"Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants","authors":"Huazhen Liu, Lakshminarayan M. Iyer, Paul Norris, Ruiying Liu, Keshun Yu, Murray Grant, L. Aravind, Aardra Kachroo, Pradeep Kachroo","doi":"10.1038/s41477-025-01906-0","DOIUrl":"10.1038/s41477-025-01906-0","url":null,"abstract":"Dietary consumption of lysine in humans leads to the biosynthesis of Δ1-piperideine-6-carboxylic acid (P6C), with elevated levels linked to the neurological disorder epilepsy. Here we demonstrate that P6C biosynthesis is also a critical component of lysine catabolism in Arabidopsis thaliana. P6C regulates vitamin B6 homeostasis, and increased P6C levels deplete B6 vitamers, resulting in compromised plant immunity. We further establish a key role for pyridoxal and pyridoxal-5-phosphate biosynthesis in plant immunity. Our analysis indicates that P6C metabolism probably evolved through combining select lysine and proline metabolic enzymes horizontally acquired from diverse bacterial sources at different points during evolution. More generally, certain enzymes from the lysine and proline metabolic pathways were probably recruited in evolution as potential guardians of B6 vitamers and for semialdehyde detoxification. This study identifies the conversion of lysine to Δ1-piperideine-6-carboxylic acid (P6C) via pipecolate oxidase as a conserved pathway in plants and humans. P6C interacts with vitamin B6, affecting its homeostasis. Imbalances in vitamin B6 homeostasis disrupt defence in plants and cause neuropathology in humans.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"263-278"},"PeriodicalIF":15.8,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143417549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of herbicide drift on plant-pollinator interactions

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01936-8

Catherine Walker

引用次数: 0

When more becomes too much in acid growth

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-14 DOI: 10.1038/s41477-025-01919-9

Pavel Krupař, Matyáš Fendrych

引用次数: 0