Nature Plants最新文献_第3页

Calcium beats for better crops

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-13 DOI: 10.1038/s41477-025-01935-9

Guillaume Tena

引用次数: 0

Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-11 DOI: 10.1038/s41477-025-01924-y

Ming Wang, Yan He, Zhenhui Zhong, Ashot Papikian, Shuya Wang, Jason Gardiner, Basudev Ghoshal, Suhua Feng, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Steven E. Jacobsen

{"title":"Histone H3 lysine 4 methylation recruits DNA demethylases to enforce gene expression in Arabidopsis","authors":"Ming Wang, Yan He, Zhenhui Zhong, Ashot Papikian, Shuya Wang, Jason Gardiner, Basudev Ghoshal, Suhua Feng, Yasaman Jami-Alahmadi, James A. Wohlschlegel, Steven E. Jacobsen","doi":"10.1038/s41477-025-01924-y","DOIUrl":"10.1038/s41477-025-01924-y","url":null,"abstract":"Patterning of DNA methylation in eukaryotic genomes is controlled by de novo methylation, maintenance mechanisms and demethylation pathways. In Arabidopsis thaliana, DNA demethylation enzymes are clearly important for shaping methylation patterns, but how they are regulated is poorly understood. Here we show that the targeting of histone H3 lysine four trimethylation (H3K4me3) with the catalytic domain of the SDG2 histone methyltransferase potently erased DNA methylation and gene silencing at FWA and also erased CG DNA methylation in many other regions of the Arabidopsis genome. This methylation erasure was completely blocked in the ros1 dml2 dml3 triple mutant lacking DNA demethylation enzymes, showing that H3K4me3 promotes the active removal of DNA methylation. Conversely, we found that the targeted removal of H3K4me3 increased the efficiency of targeted DNA methylation. These results highlight H3K4me3 as a potent anti-DNA methylation mark and also pave the way for development of more powerful epigenome engineering tools. This study revealed that targeting H3K4me3 via the H3K4 methyltransferase SDG2 activates gene expression and removes DNA methylation by recruiting DNA demethylases. Conversely, the removal of H3K4me3 synergistically enhances targeted DNA methylation.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"206-217"},"PeriodicalIF":15.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01924-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ATG8ylation of vacuolar membrane protects plants against cell wall damage

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-07 DOI: 10.1038/s41477-025-01907-z

Jose Julian, Peng Gao, Alessia Del Chiaro, Juan Carlos De La Concepcion, Laia Armengot, Marc Somssich, Heloise Duverge, Marion Clavel, Nenad Grujic, Roksolana Kobylinska, Ingo Polivka, Maarten Besten, Tonni Grube Andersen, Christian Dank, Barbara Korbei, Andreas Bachmair, Nuria S. Coll, Elena A. Minina, Joris Sprakel, Yasin Dagdas

{"title":"ATG8ylation of vacuolar membrane protects plants against cell wall damage","authors":"Jose Julian, Peng Gao, Alessia Del Chiaro, Juan Carlos De La Concepcion, Laia Armengot, Marc Somssich, Heloise Duverge, Marion Clavel, Nenad Grujic, Roksolana Kobylinska, Ingo Polivka, Maarten Besten, Tonni Grube Andersen, Christian Dank, Barbara Korbei, Andreas Bachmair, Nuria S. Coll, Elena A. Minina, Joris Sprakel, Yasin Dagdas","doi":"10.1038/s41477-025-01907-z","DOIUrl":"10.1038/s41477-025-01907-z","url":null,"abstract":"Vacuoles are essential for cellular metabolism and growth and the maintenance of internal turgor pressure. They sequester lytic enzymes, ions and secondary metabolites that, if leaked into the cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved vacuolar quality control pathway that is activated upon cell wall damage in a turgor-pressure-dependent manner. Cell wall perturbations induce a distinct modification—ATG8ylation—on the vacuolar membrane (tonoplast) that is regulated by the V-ATPase and ATG8 conjugation machinery. Genetic disruption of tonoplast ATG8ylation impairs vacuolar integrity, leading to cell death. Together, our findings reveal a homeostatic pathway that preserves vacuolar integrity upon cell wall damage. ATG8ylation of the tonoplast, triggered by cell wall damage, acts as a vital vacuolar quality control mechanism that safeguards vacuolar integrity and ensures cell survival under stress.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"321-339"},"PeriodicalIF":15.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-025-01907-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell cycle repressor promotes pollen mitosis

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-05 DOI: 10.1038/s41477-025-01929-7

Raphael Trösch

引用次数: 0

Class II kinesin-12 facilitates cell plate formation by transporting cell plate materials in the phragmoplast II类驱动蛋白-12通过在膈原体中运输细胞板材料促进细胞板的形成

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-04 DOI: 10.1038/s41477-025-01909-x

Moé Yamada, Hironori J. Matsuyama, Noriko Takeda-Kamiya, Mayuko Sato, Kiminori Toyooka

{"title":"Class II kinesin-12 facilitates cell plate formation by transporting cell plate materials in the phragmoplast","authors":"Moé Yamada, Hironori J. Matsuyama, Noriko Takeda-Kamiya, Mayuko Sato, Kiminori Toyooka","doi":"10.1038/s41477-025-01909-x","DOIUrl":"10.1038/s41477-025-01909-x","url":null,"abstract":"Cell plate formation in plants is a complex process orchestrated by the targeted delivery of Golgi-derived and endosomal vesicles containing cell plate components to the phragmoplast midzone. It has long been hypothesized that vesicles are directionally transported along phragmoplast microtubules by motor proteins. However, the mechanisms governing the accumulation and immobilization of vesicles at the phragmoplast midzone remain elusive, and the motor protein responsible has yet to be identified. Here we show that the plant-specific class II kinesin-12 (kinesin12-II) functions as a motor protein that drives vesicle transport towards the phragmoplast midzone in the moss Physcomitrium patens. In the kinesin12-II mutant, the directional movement of cell plate materials towards the midzone and their retention were abolished, resulting in delayed cell plate formation and phragmoplast disassembly. A macroscopic phenotype arising from kinesin12-II disruption was the impediment to gametophore development. We showed that this defect was attributable to the production of aneuploid and polyploid cells in the early gametophore, where chromosome missegregation and cytokinesis failure occurred. These findings suggest that plant kinesin-12 has evolved to acquire a unique and critical function that facilitates cell plate formation in the presence of phragmoplasts. Class II kinesin-12 is responsible for transporting vesicles containing cell plate materials along phragmoplast microtubules towards the midzone, facilitating efficient cell plate formation during cytokinesis and enabling sequential cell division during multicellular organ development.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"340-358"},"PeriodicalIF":15.8,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143083417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1

IF 18 1区生物学

Nature Plants Pub Date : 2025-02-03 DOI: 10.1038/s41477-024-01896-5

Ziyin Ren, Ling Zhang, Haizhen Li, Mi Yang, Xuesong Wu, Runxu Hu, Jingjing Lu, Hui Wang, Xinying Wu, Zhijuan Wang, Xia Li

{"title":"The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1","authors":"Ziyin Ren, Ling Zhang, Haizhen Li, Mi Yang, Xuesong Wu, Runxu Hu, Jingjing Lu, Hui Wang, Xinying Wu, Zhijuan Wang, Xia Li","doi":"10.1038/s41477-024-01896-5","DOIUrl":"https://doi.org/10.1038/s41477-024-01896-5","url":null,"abstract":"<p>Legumes form root nodules with symbiotic nitrogen-fixing rhizobacteria, which require ample iron to ensure symbiosis establishment and efficient nitrogen fixation. The functions and mechanisms of iron in nitrogen-fixing nodules are well established. However, the role of iron and the mechanisms by which legumes sense iron and incorporate this cue into nodulation signalling pathways remain unclear. Here we show that iron is a key driver of nodulation because symbiotic nodules cannot form without iron, even under conditions of sufficient light and low nitrogen. We further identify an iron optimum for soybean nodulation and the iron sensor BRUTUS A (BTSa) which acts as a hub for integrating iron and nodulation cues. BTSa is induced by rhizobia, binds to and is stabilized by iron. In turn, BTSa stabilizes and enhances the transcriptional activation activity of pro-nodulation transcription factor NSP1a by monoubiquitination from its RING domain and consequently activates nodulation signalling. Monoubiquitination of NSP1 by BTS is conserved in legumes to trigger nodulation under iron sufficiency. Thus, iron status is an essential cue to trigger nodulation and BTSa integrates cues from rhizobial infection and iron status to orchestrate host responses towards establishing symbiotic nitrogen fixation.</p>","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 1","pages":""},"PeriodicalIF":18.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudouridine is the hidden language of plant RNA translation

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-02-03 DOI: 10.1038/s41477-025-01926-w

Patricia Baldrich

引用次数: 0

Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-01-31 DOI: 10.1038/s41477-025-01908-y

Yiling Fang, Yu Tang, Peiqiao Xie, Kendall Hsieh, Heejae Nam, Min Jia, Andres V. Reyes, Yuchen Liu, Shouling Xu, Xiaosa Xu, Yangnan Gu

{"title":"Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division","authors":"Yiling Fang, Yu Tang, Peiqiao Xie, Kendall Hsieh, Heejae Nam, Min Jia, Andres V. Reyes, Yuchen Liu, Shouling Xu, Xiaosa Xu, Yangnan Gu","doi":"10.1038/s41477-025-01908-y","DOIUrl":"10.1038/s41477-025-01908-y","url":null,"abstract":"The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here we report that Plant Nuclear Envelope Transmembrane 1 (PNET1), a transmembrane nucleoporin, is an adaptable NPC component that is mainly expressed in actively dividing cells. PNET1’s selective incorporation into the NPC is required for rapid cell growth in highly proliferative meristem and callus tissues in Arabidopsis. We demonstrate that the cell cycle-dependent phosphorylation of PNET1 coordinates mitotic disassembly and post-mitotic reassembly of NPCs during the cell cycle. PNET1 hyperphosphorylation disrupts its interaction with the NPC scaffold, facilitating NPC dismantling and nuclear membrane breakdown to trigger mitosis. In contrast, nascent, unphosphorylated PNET1 is incorporated into the nuclear pore membrane in the daughter cells, where it restores interactions with scaffolding nucleoporins for NPC reassembly. The expression of the human PNET1 homologue is required for and markedly upregulated during cancer cell growth, suggesting that PNET1 plays a conserved role in facilitating rapid cell division during open mitosis in highly proliferative tissues. The nuclear pore complex is a cornerstone of eukaryotic cell function. This study identifies a cell-type-specific nucleoporin that regulates mitotic disassembly and reassembly of the nuclear pore complex, serving as a critical mechanism to promote rapid cell division.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 2","pages":"295-308"},"PeriodicalIF":15.8,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genome analysis unravels adaptive evolution in Dendrobium orchids

IF 18 1区生物学

Nature Plants Pub Date : 2025-01-30 DOI: 10.1038/s41477-025-01925-x

引用次数: 0

Author Correction: Refining polyploid breeding in sweet potato through allele dosage enhancement

IF 15.8 1区生物学

Nature Plants Pub Date : 2025-01-28 DOI: 10.1038/s41477-025-01927-9

Xiangbo Zhang, Chaochen Tang, Bingzhi Jiang, Rong Zhang, Ming Li, Yaoyao Wu, Zhufang Yao, Lifei Huang, Zhongxia Luo, Hongda Zou, Yiling Yang, Minyi Wu, Ao Chen, Shan Wu, Xingliang Hou, Xu Liu, Zhangjun Fei, Junjie Fu, Zhangying Wang

引用次数: 0