Nature Plants最新文献_第4页

A sexually and vegetatively reproducible diploid seedless watermelon inducer via ClHAP2 mutation 通过 ClHAP2 基因突变诱导有性和无性繁殖的二倍体无籽西瓜

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01799-5

Xiner Chen, Yuxiu Li, Man Liu, Gongli Ai, Xian Zhang, Jiafa Wang, Shujuan Tian, Li Yuan

引用次数: 0

Nuclear-localized pyruvate kinases control phosphorylation of histone H3 on threonine 11 核定位丙酮酸激酶控制组蛋白 H3 苏氨酸 11 上的磷酸化

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01821-w

Pengcheng Hu, Yanmei Xu, Yanhua Su, Yuxin Wang, Yan Xiong, Yong Ding

引用次数: 0

The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair 组蛋白去甲基化酶 JMJ27 在紫外线诱导的 H3K9me2 景观调节过程中发挥作用，并促进光损伤修复

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01814-9

Philippe Johann to Berens, Jackson Peter, Sandrine Koechler, Mathieu Bruggeman, Sébastien Staerck, Jean Molinier

{"title":"The histone demethylase JMJ27 acts during the UV-induced modulation of H3K9me2 landscape and facilitates photodamage repair","authors":"Philippe Johann to Berens, Jackson Peter, Sandrine Koechler, Mathieu Bruggeman, Sébastien Staerck, Jean Molinier","doi":"10.1038/s41477-024-01814-9","DOIUrl":"https://doi.org/10.1038/s41477-024-01814-9","url":null,"abstract":"Plants have evolved sophisticated DNA repair mechanisms to cope with the deleterious effects of ultraviolet (UV)-induced DNA damage. Indeed, DNA repair pathways cooperate with epigenetic-related processes to efficiently maintain genome integrity. However, it remains to be deciphered how photodamages are recognized within different chromatin landscapes, especially in compacted genomic regions such as constitutive heterochromatin. Here we combined cytogenetics and epigenomics to identify that UV-C irradiation induces modulation of the main epigenetic mark found in constitutive heterochromatin, H3K9me2. We demonstrated that the histone demethylase, Jumonji27 (JMJ27), contributes to the UV-induced reduction of H3K9me2 content at chromocentres. In addition, we identified that JMJ27 forms a complex with the photodamage recognition factor, DNA Damage Binding protein 2 (DDB2), and that the fine-tuning of H3K9me2 contents orchestrates DDB2 dynamics on chromatin in response to UV-C exposure. Hence, this study uncovers the unexpected existence of an interplay between photodamage repair and H3K9me2 homeostasis.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector 植物 PR1 挽救了真菌效应物对质体铁硫蛋白的凝结作用

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01811-y

Jingtao Li, Limei Yang, Shuzhi Ding, Mingming Gao, Yu Yan, Gang Yu, Yaning Zheng, Wenxing Liang

引用次数: 0

Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway Rubisco 为 2-C-methyl-d-erythritol-4-phosphate 途径提供丙酮酸

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-10-04 DOI: 10.1038/s41477-024-01791-z

Sonia E. Evans, Yuan Xu, Matthew E. Bergman, Scott A. Ford, Yingxia Liu, Thomas D. Sharkey, Michael A. Phillips

{"title":"Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway","authors":"Sonia E. Evans, Yuan Xu, Matthew E. Bergman, Scott A. Ford, Yingxia Liu, Thomas D. Sharkey, Michael A. Phillips","doi":"10.1038/s41477-024-01791-z","DOIUrl":"10.1038/s41477-024-01791-z","url":null,"abstract":"RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (Rubisco) produces pyruvate in the chloroplast through β-elimination of the aci-carbanion intermediate1. Here we show that this side reaction supplies pyruvate for isoprenoid, fatty acid and branched-chain amino acid biosynthesis in photosynthetically active tissue. 13C labelling studies of intact Arabidopsis plants demonstrate that the total carbon commitment to pyruvate is too large for phosphoenolpyruvate to serve as a precursor. Low oxygen stimulates Rubisco carboxylase activity and increases pyruvate production and flux through the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, which supplies the precursors for plastidic isoprenoid biosynthesis2,3. Metabolome analysis of mutants defective in phosphoenolpyruvate or pyruvate import and biochemical characterization of isolated chloroplasts further support Rubisco as the main source of pyruvate in chloroplasts. Seedlings incorporated exogenous,13C-labelled pyruvate into MEP pathway intermediates, while adult plants did not, underscoring the developmental transition in pyruvate sourcing. Rubisco β-elimination leading to pyruvate constituted 0.7% of the product profile in in vitro assays, which translates to 2% of the total carbon leaving the Calvin–Benson–Bassham cycle. These insights solve the “pyruvate paradox”4, improve the fit of metabolic models for central metabolism and connect the MEP pathway directly to carbon assimilation. As nature’s most important enzyme, Rubisco fixes carbon dioxide in the Calvin–Benson–Bassham cycle. Its lesser-known side job is supplying pyruvate in the chloroplast, an observation that solves a long-standing paradox of central metabolism.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142374125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular basis of plastoquinone reduction in plant cytochrome b6f 植物细胞色素 b6f 中质子醌还原的分子基础

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-03 DOI: 10.1038/s41477-024-01804-x

Sebastian Pintscher, Rafał Pietras, Bohun Mielecki, Mateusz Szwalec, Anna Wójcik-Augustyn, Paulina Indyka, Michał Rawski, Łukasz Koziej, Marcin Jaciuk, Grzegorz Ważny, Sebastian Glatt, Artur Osyczka

{"title":"Molecular basis of plastoquinone reduction in plant cytochrome b6f","authors":"Sebastian Pintscher, Rafał Pietras, Bohun Mielecki, Mateusz Szwalec, Anna Wójcik-Augustyn, Paulina Indyka, Michał Rawski, Łukasz Koziej, Marcin Jaciuk, Grzegorz Ważny, Sebastian Glatt, Artur Osyczka","doi":"10.1038/s41477-024-01804-x","DOIUrl":"https://doi.org/10.1038/s41477-024-01804-x","url":null,"abstract":"A multi-subunit enzyme, cytochrome b6f (cytb6f), provides the crucial link between photosystems I and II in the photosynthetic membranes of higher plants, transferring electrons between plastoquinone (PQ) and plastocyanin. The atomic structure of cytb6f is known, but its detailed catalytic mechanism remains elusive. Here we present cryogenic electron microscopy structures of spinach cytb6f at 1.9 Å and 2.2 Å resolution, revealing an unexpected orientation of the substrate PQ in the haem ligand niche that forms the PQ reduction site (Qn). PQ, unlike Qn inhibitors, is not in direct contact with the haem. Instead, a water molecule is coordinated by one of the carbonyl groups of PQ and can act as the immediate proton donor for PQ. In addition, we identify water channels that connect Qn with the aqueous exterior of the enzyme, suggesting that the binding of PQ in Qn displaces water through these channels. The structures confirm large movements of the head domain of the iron–sulfur protein (ISP-HD) towards and away from the plastoquinol oxidation site (Qp) and define the unique position of ISP-HD when a Qp inhibitor (2,5-dibromo-3-methyl-6-isopropylbenzoquinone) is bound. This work identifies key conformational states of cytb6f, highlights fundamental differences between substrates and inhibitors and proposes a quinone–water exchange mechanism.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142369347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Megafire smoke exposure jeopardizes tree carbohydrate reserves and yield 特大火灾的烟雾危害树木的碳水化合物储备和产量

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-02 DOI: 10.1038/s41477-024-01819-4

Jessica Orozco, Paula Guzmán-Delgado, Maciej A. Zwieniecki

引用次数: 0

From RdDM to plant defence 从 RdDM 到植物防御

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-30 DOI: 10.1038/s41477-024-01806-9

Claudia Martinho

引用次数: 0

A novel in vivo genome editing doubled haploid system for Zea mays L. 用于玉米的新型体内基因组编辑加倍单倍体系统

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01795-9

Huaxun Ye, Mei Louden, Jon A. T. Reinders

{"title":"A novel in vivo genome editing doubled haploid system for Zea mays L.","authors":"Huaxun Ye, Mei Louden, Jon A. T. Reinders","doi":"10.1038/s41477-024-01795-9","DOIUrl":"10.1038/s41477-024-01795-9","url":null,"abstract":"Doubled haploid (DH) technologies accelerate maize inbred development. Recently, methods using CRISPR–Cas have created gene-edited maize DH populations, albeit with relatively low editing frequencies. Restoring fertility via haploid chromosome doubling remains a critically important production constraint. Thus, improved editing and chromosome doubling outcomes are needed. Here we obtained maternally derived diploid embryos in vivo by ectopically co-expressing Zea mays BABY BOOM and cyclin D-like gene products within unfertilized egg cells. When combined with gene editing, the in vivo method enables the production of mature seed with a maternally derived, gene-edited diploid embryo without requiring in vitro tissue culture methods nor the use of a chemical chromosome doubling agent. In summary, we report a novel approach for creating gene-edited maize DH populations that we expect can accelerate genetic gain in a scalable, cost-effective manner. Ye et al. use a novel genetic haploid genome doubling method with parthenogenesis and gene editing to produce edited, maternally derived di-haploid progeny. A truncated BABY BOOM peptide confers both parthenogenesis and haploid genome doubling.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide 逆转录转座子驱动的FLC环境调控导致对除草剂的适应性反应

IF 18 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01807-8

Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana

{"title":"Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide","authors":"Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana","doi":"10.1038/s41477-024-01807-8","DOIUrl":"https://doi.org/10.1038/s41477-024-01807-8","url":null,"abstract":"The mobilization of transposable elements is a potent source of mutations. In plants, several stransposable elements respond to external cues, fuelling the hypothesis that natural transposition can create environmentally sensitive alleles for adaptation. Here we report on the detailed characterization of a retrotransposon insertion within the first intron of the Arabidopsis floral-repressor gene FLOWERING LOCUS C (FLC) and the discovery of its role for adaptation. The insertion mutation augments the environmental sensitivity of FLC by affecting the balance between coding and non-coding transcripts in response to stress, thus expediting flowering. This balance is modulated by DNA methylation and orchestrated by IBM2, a factor involved in the processing of intronic heterochromatic sequences. The stress-sensitive allele of FLC has spread across populations subjected to recurrent chemical weeding, and we show that retrotransposon-driven acceleration of the life cycle represents a rapid response to herbicide application. Our work provides a compelling example of a transposable element-driven environmentally sensitive allele that confers an adaptive response in nature.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0