Nature Plants最新文献_第5页

Kinesins control male germ unit assembly for sperm delivery in Arabidopsis 运动蛋白控制拟南芥雄性生殖单位组装以实现精子的传递。

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-18 DOI: 10.1038/s41477-025-02084-9

Shu Chang, Iftikhar Ali, Peng-Min Zhou, Haisu Cheng, Xin Liang, Hua-Mao Wu, Dong-Qiao Shi, Hong-Ju Li, Wei-Cai Yang

{"title":"Kinesins control male germ unit assembly for sperm delivery in Arabidopsis","authors":"Shu Chang, Iftikhar Ali, Peng-Min Zhou, Haisu Cheng, Xin Liang, Hua-Mao Wu, Dong-Qiao Shi, Hong-Ju Li, Wei-Cai Yang","doi":"10.1038/s41477-025-02084-9","DOIUrl":"10.1038/s41477-025-02084-9","url":null,"abstract":"Unlike flagellated sperm in animals, which use molecular motors for motility, immotile sperm cells of angiosperms rely on cytoplasmic transport within pollen tubes to reach female gametes for fertilization. However, the mechanism underlying sperm cell transport in angiosperms remains unknown. Since the 1970s it has been observed that the two sperm cells, or their progenitor generative cell, are transported together with the pollen vegetative nucleus as part of an aggregated structure called the male germ unit, which forms within the pollen cytoplasm. Here, using super-resolution and live-cell imaging, we show that two kinesins, HUG1 and HUG2, form a kinesin cage encasing a microtubule cage around the generative cell or sperm cells and vegetative nucleus, tethering them into a single unit during Arabidopsis pollen development. Loss of HUG proteins disrupts male germ unit organization, leading to failed sperm delivery and complete plant sterility. These findings uncover the genetic and cellular basis of male germ unit organization and highlight its essential role in sperm transport for plant fertilization. Here Chang et al. found that two kinesins, HUG1 and HUG2, form a kinesin cage encasing the vegetative nucleus and a distinctive double-layered kinesin microtubule cage around the generative cell, tethering them into a male germ unit and aiding sperm delivery.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 9","pages":"1798-1809"},"PeriodicalIF":13.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity 液泡分选受体协调溶解性液泡和自噬运输，实现植物效应触发免疫。

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-18 DOI: 10.1038/s41477-025-02077-8

Dongmei Zhu, Shuai Hu, Wenhan Cao, Yanli Gao, Yan Li, Juan Xu, Baiying Li, Liwen Jiang, Enrique Rojo, Jinbo Shen

{"title":"Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity","authors":"Dongmei Zhu, Shuai Hu, Wenhan Cao, Yanli Gao, Yan Li, Juan Xu, Baiying Li, Liwen Jiang, Enrique Rojo, Jinbo Shen","doi":"10.1038/s41477-025-02077-8","DOIUrl":"10.1038/s41477-025-02077-8","url":null,"abstract":"Vacuolar sorting receptors (VSRs) are involved in sorting soluble vacuolar proteins during normal plant growth and development, but their role in plant stress responses remains largely unexplored. Here we report that a subgroup of the Arabidopsis thaliana VSR genes are transcriptionally induced during infection with avirulent Pseudomonas syringae strains, leading to higher VSR protein accumulation. We demonstrate that the pathogen-responsive VSR1, VSR5, VSR6 and VSR7 genes function redundantly in sorting vacuolar death-related enzymes induced during bacterial infection. Moreover, VSRs are required for fusion of the tonoplast with the plasma membrane and the subsequent release of vacuolar contents into the apoplast, where bacterial pathogens reside. Indeed, dysfunction of this subgroup of VSRs blocks hypersensitive cell death and leads to stronger disease symptoms and higher bacterial loads, revealing their essential role in defence against avirulent bacterial infection. Intriguingly, their disruption also leads to defects in autophagy, impairing autophagosome-mediated degradation of bacterial effector proteins. Collectively, our results show that VSR1, VSR5, VSR6 and VSR7 are key regulators of plant effector-triggered immunity (ETI) by orchestrating receptor-mediated vacuolar sorting of immunity-related proteins, tonoplast to plasma membrane fusion, and autophagic degradation of effector proteins. Vacuolar sorting receptors (VSRs) play crucial roles in plant stress responses. This study reveals that VSRs orchestrate the vacuolar sorting of immune-related proteins and autophagic degradation of bacterial effectors in effector-triggered immunity.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 9","pages":"1827-1846"},"PeriodicalIF":13.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants 陆生植物光合蛋白组装依赖的翻译反馈调控。

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-18 DOI: 10.1038/s41477-025-02074-x

Rabea Ghandour, Yang Gao, Stephanie Ruf, Ralph Bock, Reimo Zoschke

{"title":"Assembly-dependent translational feedback regulation of photosynthetic proteins in land plants","authors":"Rabea Ghandour, Yang Gao, Stephanie Ruf, Ralph Bock, Reimo Zoschke","doi":"10.1038/s41477-025-02074-x","DOIUrl":"10.1038/s41477-025-02074-x","url":null,"abstract":"In the green alga Chlamydomonas reinhardtii, the synthesis of chloroplast-encoded photosynthetic subunits is feedback regulated by their protein complex assembly state. This regulation is known as control by epistasy of synthesis (CES) and matches subunit synthesis with requirements of complex assembly in photosystem II (PSII), the cytochrome b6f complex (Cyt b6f), photosystem I (PSI), ATP synthase and Rubisco. In embryophytes, CES was only described for Rubisco, raising the question of whether CES exists for components of the photosynthetic electron transport chain in land plants. Here we systematically examined land plant mutants with assembly defects in PSII, Cyt b6f, PSI, ATP synthase, NAD(P)H dehydrogenase-like (NDH) complex and Rubisco for feedback regulation. We confirmed the CES in Rubisco and provide evidence for translational feedback regulation in PSII, involving psbA, psbB and psbD, and in Cyt b6f, connecting petA and petB. Our results also point to potential feedback regulation between ATP synthase subunits. Most of these regulatory connections are not conserved between algae and embryophytes. We did not find evidence for CES in land plant PSI or NDH complex assembly. Our results, however, indicate a regulatory connection between PSII and PSI. Overall, we revealed commonalities and differences in assembly-dependent feedback regulation of photosynthetic complexes between embryophytes and green algae. Algae fine-tune protein synthesis to assembly demands of photosynthetic complexes. In land plants, this feedback control is conserved in Rubisco and partly in photosystem II, but varies or is absent in other complexes, revealing divergent evolution.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 9","pages":"1920-1938"},"PeriodicalIF":13.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microrchidia ATPases and DNA 6mA demethylase ALKBH1 act antagonistically on PRC2 to control chromatin structure and stress tolerance 微鼠ATPases和DNA 6mA去甲基化酶ALKBH1拮抗PRC2，控制染色质结构和胁迫耐受性

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-15 DOI: 10.1038/s41477-025-02048-z

Xinran Zhang, Qingxiao Jia, Wentao Wang, Yu Chang, Qian Liu, Chen Chu, Jingjing Tian, Meng Yuan, Yu Zhao, Dao-Xiu Zhou

{"title":"Microrchidia ATPases and DNA 6mA demethylase ALKBH1 act antagonistically on PRC2 to control chromatin structure and stress tolerance","authors":"Xinran Zhang, Qingxiao Jia, Wentao Wang, Yu Chang, Qian Liu, Chen Chu, Jingjing Tian, Meng Yuan, Yu Zhao, Dao-Xiu Zhou","doi":"10.1038/s41477-025-02048-z","DOIUrl":"10.1038/s41477-025-02048-z","url":null,"abstract":"The Microrchidia (MORC) proteins are conserved GHKL-type ATPases required for chromatin condensation and gene silencing in animals and plants. Here we show that MORC proteins function with Polycomb-Repressive Complex 2 (PRC2) to control chromatin structure, gene expression and stress responses in rice. Rice MORC6b interacts with and stabilizes PRC2 for trimethylated histone H3 lysine 27 (H3K27me3) deposition preferentially at bivalent domains marked by both H3K4me3 and H3K27me3 to repress genes enriched for stress responses. The MORC-binding sites perfectly overlap with a set of PRC2 targets and colocalize with chromatin loop boundaries. High-throughput chromatin conformation capture combined with chromatin immunoprecipitation (Hi-ChIP) analysis revealed that the morc mutation reduces the number of H3K27me3-marked chromatin loops mainly at bivalent domains compressing many defence-related genes and affects rice plant tolerance to biotic and abiotic stresses. MORC function in H3 K27 trimethylation and gene expression is partly inhibited by ALKBH1, a DNA 6mA demethylase that impairs PRC2 binding and H3K27me3 deposition at bivalent chromatin domains and has an opposite function to MORC in stress responses. These findings identify MORCs and ALKBH1 as an antagonistic couple controlling PRC2 function in regulating chromatin structure and gene expression preferentially at bivalent chromatin domains for stress responses, which is instructive for understanding the regulation of chromatin dynamics in other eukaryotic organisms. In this work, Zhang, Jia, Wang et al. show that MORCs stabilize PRC2 preferentially at bivalent domains to maintain high levels of H3 K27 trimethylation and form chromatin loops for gene repression and stress tolerance, and are counteracted by ALKBH1, a DNA 6mA demethylase.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 8","pages":"1591-1607"},"PeriodicalIF":13.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144851402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A conserved RNA motif guides DCL1-mediated cleavage in plant microRNA biogenesis 一个保守的RNA基序引导dcl1介导的植物microRNA生物发生中的裂解

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-14 DOI: 10.1038/s41477-025-02081-y

引用次数: 0

A rack for the hook 挂钩子的架子

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-14 DOI: 10.1038/s41477-025-02098-3

Raphael Trösch

引用次数: 0

ARF degradation fine-tunes auxin response in land plants ARF降解微调陆地植物的生长素反应

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-13 DOI: 10.1038/s41477-025-02092-9

Suruchi Roychoudhry, Marta Del Bianco, Stefan Kepinski

引用次数: 0

Key RNA elements influencing DCL1 cleavage in plant microRNA biogenesis 植物microRNA生物发生中影响DCL1裂解的关键RNA元件

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-12 DOI: 10.1038/s41477-025-02067-w

Thi Nhu-Y Le, Trung Duc Nguyen, Yu Yu, Wutao Xie, Xuemei Chen, Tuan Anh Nguyen

{"title":"Key RNA elements influencing DCL1 cleavage in plant microRNA biogenesis","authors":"Thi Nhu-Y Le, Trung Duc Nguyen, Yu Yu, Wutao Xie, Xuemei Chen, Tuan Anh Nguyen","doi":"10.1038/s41477-025-02067-w","DOIUrl":"10.1038/s41477-025-02067-w","url":null,"abstract":"DICER-LIKE 1 (DCL1), a plant-specific RNase III enzyme, is fundamental to post-transcriptional gene regulation mediated by microRNAs (miRNAs). DCL1 processes precursor miRNAs into mature miRNAs, typically 20–22 nucleotides long. Despite its crucial role, the RNA elements that guide DCL1’s cleavage site selection have remained largely uncharacterized. In this study, we employed a high-throughput sequencing approach to analyse Arabidopsis thaliana DCL1 cleavage patterns on over 46,000 short hairpin RNA sequences previously studied with human DICER. Our analyses revealed that DCL1 cleavage preferences are governed by specific secondary RNA structures and sequence motifs, among which a particular RNA element, designated the GHR motif, emerged as pivotal. This motif remarkably influences cleavage site selection independently of the double-stranded RNA-binding domains and helicase domains of DCL1, operating primarily through the RNase IIIDa domain. The GHR motif is evolutionarily conserved across plant species and is essential for the precise cleavage of various plant precursor miRNAs. Our findings also suggest a role for the GHR motif in the biogenesis of non-canonical 22-nucleotide miRNAs, expanding its functional impact. These insights deepen our understanding of the molecular mechanisms underlying DCL1’s specificity and highlight its integral role in miRNA maturation and gene regulatory networks in plants. This study applied a high-throughput sequencing approach to analyse Arabidopsis DCL1 cleavage. DCL1 cleavage preferences are governed by the GHR motif, which is conserved across plant species and has a role in the biogenesis of non-canonical 22-nucleotide microRNAs.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"11 8","pages":"1528-1543"},"PeriodicalIF":13.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144819943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the complexity of the hexaploid sweet potato genome 破解甘薯六倍体基因组的复杂性

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-11 DOI: 10.1038/s41477-025-02090-x

引用次数: 0

Cooling down transposon mobility 冷却转座子迁移率

IF 13.6 1区生物学

Nature Plants Pub Date : 2025-08-11 DOI: 10.1038/s41477-025-02087-6

Etienne Bucher

引用次数: 0