Nature Plants最新文献_第5页

Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice 基于细胞的CRISPRa筛选鉴定了介导水稻广谱真菌抗性的乳突特异性受体激酶

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-07 DOI: 10.1038/s41477-026-02385-7

Feng-Zhu Wang (, ), Ying Bao (, ), Jiehua Qiu (, ), Mo-Xian Chen (, ), He Jiang (, ), Zhenxiang Li (, ), Xiangyu Xiong (, ), Xianya Jiang (, ), Yanjun Kou (, ), Jian-Feng Li (, )

{"title":"Cell-based CRISPRa screen identifies a papillae-specific receptor kinase mediating broad-spectrum fungal resistance in rice","authors":"Feng-Zhu Wang \u0000 (, ), Ying Bao \u0000 (, ), Jiehua Qiu \u0000 (, ), Mo-Xian Chen \u0000 (, ), He Jiang \u0000 (, ), Zhenxiang Li \u0000 (, ), Xiangyu Xiong \u0000 (, ), Xianya Jiang \u0000 (, ), Yanjun Kou \u0000 (, ), Jian-Feng Li \u0000 (, )","doi":"10.1038/s41477-026-02385-7","DOIUrl":"10.1038/s41477-026-02385-7","url":null,"abstract":"Gain-of-function screens enable the discovery of gene functions and candidate targets for molecular breeding. Here we developed a protoplast-based dCas9-TV-mediated CRISPR activation screen in rice and identified OsTV1, an epidermal papillae-specific receptor kinase, as a positive regulator of fungal resistance. OsTV1 overexpression enhanced silicon deposition, leaf rigidity and basal defence gene expression, conferring broad-spectrum fungal resistance without compromising growth. This study establishes a high-throughput gain-of-function screen adaptable for investigating diverse cellular processes in plants and provides insights into the roles of silicon in rice defence. The authors develop a protoplast-based dCas9-TV-mediated CRISPR activation screen in rice, identifying a papillae-specific receptor kinase that positively regulates fungal resistance via silicon-mediated physical and biochemical defences.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"12 10","pages":"1913-1922"},"PeriodicalIF":15.0,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148899585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mapping genetic modifiers of epimutation rates identifies VIM2/4 as dosage-sensitive negative regulators of CG methylation maintenance 绘制细胞增殖率的遗传修饰因子，确定VIM2/4是CG甲基化维持的剂量敏感负调节因子。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-03 DOI: 10.1038/s41477-026-02396-4

Zhilin Zhang, Wilma Wanney, Yangyang Xu, Johan Zicola, Angela M. Hancock, Robert J. Schmitz, Frank Johannes

{"title":"Mapping genetic modifiers of epimutation rates identifies VIM2/4 as dosage-sensitive negative regulators of CG methylation maintenance","authors":"Zhilin Zhang, Wilma Wanney, Yangyang Xu, Johan Zicola, Angela M. Hancock, Robert J. Schmitz, Frank Johannes","doi":"10.1038/s41477-026-02396-4","DOIUrl":"10.1038/s41477-026-02396-4","url":null,"abstract":"Spontaneous epimutations are stochastic gains and losses of cytosine methylation that arise from imperfect maintenance across cell divisions. At CG sites, such epimutations can be inherited across generations in plants and constitute a major source of CG methylation (mCG) diversity. However, why the fidelity of mCG inheritance varies among genotypes, and how this variation relates to steady-state mCG levels, remains poorly understood. Here we tracked DNA methylation over 10 generations in ~400 mutation-accumulation lines derived from ~70 Arabidopsis thaliana Ler × Cvi recombinant inbred founders. By treating methylation gain and loss rates as quantitative molecular traits, we mapped a major-effect locus to a Cvi-derived deletion between VARIANT IN METHYLATION (VIM)2 and VIM4, two key components of the METHYLTRANSFERASE 1-dependent mCG maintenance pathway. Lines carrying this deletion showed elevated VIM2/4 (VIM2 and VIM4) expression, a rapid shift of genome-wide mCG towards a lower steady state and reduced fidelity of methylation inheritance across generations. Complementary overexpression and loss-of-function experiments identify VIM2/4 as dosage-sensitive negative regulators of mCG maintenance, in contrast to the canonical positive role of VIM-family proteins in mCG. Together, our results support a punctuated-equilibrium model of DNA methylome evolution, in which naturally segregating modifiers of mCG homeostasis can produce abrupt shifts in methylation state and alter the rate at which heritable epigenetic variation accumulates in plant genomes. This study shows that epimutation rates are heritable quantitative traits and identifies VIM2 and VIM4 as dosage-sensitive negative regulators of CG methylation levels and inheritance fidelity.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"12 10","pages":"1976-1994"},"PeriodicalIF":15.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41477-026-02396-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deletion loosens methylation memory 缺失会使甲基化记忆松动。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-03 DOI: 10.1038/s41477-026-02403-8

Claude Becker

引用次数: 0

Brassinosteroids boost sugar production by inhibiting PCK phosphorylation 油菜素内酯通过抑制PCK磷酸化促进糖的产生。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-02 DOI: 10.1038/s41477-026-02378-6

引用次数: 0

Breaking the apomixis trade-off 打破无融合的权衡。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-02 DOI: 10.1038/s41477-026-02404-7

Tengyu Li, Baicui Wang, Yazhong Wang

引用次数: 0

A domestication-selected CIPK–MYB–HAK module improves salt tolerance in modern maize 驯化选择的CIPK-MYB-HAK模块提高了现代玉米的耐盐性。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-01 DOI: 10.1038/s41477-026-02384-8

Xiaoyan Liang, Pan Yin, Chenhuan Guo, Ziying Zhao, Rumeng Liu, Yalin Li, Chuang Zhang, Feng Tian, Xiangdong Fu, Caifu Jiang

{"title":"A domestication-selected CIPK–MYB–HAK module improves salt tolerance in modern maize","authors":"Xiaoyan Liang, Pan Yin, Chenhuan Guo, Ziying Zhao, Rumeng Liu, Yalin Li, Chuang Zhang, Feng Tian, Xiangdong Fu, Caifu Jiang","doi":"10.1038/s41477-026-02384-8","DOIUrl":"10.1038/s41477-026-02384-8","url":null,"abstract":"Domestication has profoundly reshaped the maize genome, yet its impact on abiotic stress resilience remains largely unresolved. Here we show that modern maize exhibits markedly greater salt tolerance than its wild progenitor, teosinte. Using a maize–teosinte (W22-T8759) recombinant inbred line population, we identify the transcriptional repressor MYB28 as a major genetic determinant underlying this divergence. MYB28 negatively regulates salt tolerance by impairing shoot Na+ exclusion. In modern maize (W22), however, salt stress activates CIPK20, which phosphorylates MYB28 to relieve its repression. A non-synonymous single nucleotide polymorphism (SNP1409T) disrupts this regulation in teosinte (T8759), conferring salt sensitivity. We further identify HAK4 as a downstream target of the CIPK20–MYB28 pathway, mediating shoot Na+ exclusion and salt adaptation. Population genomic and cross-species analyses revealed that the salt-tolerant SNP1409T MYB28 allele originated in ancestral teosinte and was positively selected during domestication, increasing yield in salt-affected fields. Together, our findings uncover a domestication-selected CIPK20–MYB28–HAK4 module that provides mechanistic insights into the evolutionary trajectory of salt tolerance, and identifies valuable targets for developing salt-resilient cultivars. Modern maize has evolved enhanced salt tolerance via a CIPK20–MYB28–HAK4 regulatory module, absent in its wild progenitor teosinte. These findings highlight the evolutionary trajectory underlying maize’s adaptation to salinity.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"12 10","pages":"2010-2025"},"PeriodicalIF":15.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant immune receptors built in silico and evolved in vivo 植物免疫受体在硅中构建并在体内进化。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-01 DOI: 10.1038/s41477-026-02398-2

Jonathan D. G. Jones, He Zhao

引用次数: 0

Evolution and implementation of EU regulation on new genomic techniques 欧盟新基因组技术法规的发展和实施。

IF 15 1区生物学

Nature Plants Pub Date : 2026-09-01 DOI: 10.1038/s41477-026-02402-9

Teodoro Cardi, Alessandro Vitale

引用次数: 0

Optimization of the Isolation and Purification of Formononetin from Lycopus lucidus Turcz. var. hirtus Regel, Structural Identification, and Its In Vitro Anti-Inhibitory Effects in TNF-α-Induced HFLS-RA Cells 绿番茄中刺芒柄花素的分离纯化工艺优化。hirtus Regel，结构鉴定及其对TNF-α-诱导的HFLS-RA细胞的体外抗抑制作用

1区生物学

Nature Plants Pub Date : 2026-08-29 DOI: 10.3390/plants15172654

Feiran Hu, Wenzhao Zhou, Nong Zhou, Lanlan Wan, Linyun Pu, Caiyun Fu, Hua Zhang, Junsheng Qi

{"title":"Optimization of the Isolation and Purification of Formononetin from Lycopus lucidus Turcz. var. hirtus Regel, Structural Identification, and Its In Vitro Anti-Inhibitory Effects in TNF-α-Induced HFLS-RA Cells","authors":"Feiran Hu, Wenzhao Zhou, Nong Zhou, Lanlan Wan, Linyun Pu, Caiyun Fu, Hua Zhang, Junsheng Qi","doi":"10.3390/plants15172654","DOIUrl":"https://doi.org/10.3390/plants15172654","url":null,"abstract":"Lycopus lucidus Turcz. var. hirtus Regel (L. lucidus var. hirtus) is a medicinal herb traditionally used in Chinese folk medicine for the treatment of inflammatory disorders, joint pain, and rheumatoid arthritis (RA)-like symptoms. However, systematic research on the bioactive flavonoids of this herb in RA-related cellular models is limited. The aim of this study was to isolate the main flavonoid from this herb and evaluate its in vitro anti-inhibitory effects in TNF-α-induced human RA fibroblast-like synoviocytes (HFLS-RA). Flavonoids were extracted, purified, and isolated using chromatographic techniques. The structure was elucidated by spectroscopic analysis. The effects of the isolated compound were assessed in a TNF-α-induced HFLS-RA cell model by evaluating pro-inflammatory cytokine secretion and cell proliferation/metabolic activity. A main flavonoid was identified as formononetin. Formononetin exhibited antioxidant activity and inhibited TNF-α-provoked inflammatory responses in HFLS-RA cells under cell-safe concentrations, as evidenced by reduced secretion of IL-6, IL-8, and IL-1β and suppression of TNF-α-induced hyperproliferation. In conclusion, formononetin is a major flavonoid constituent of L. lucidus var. hirtus, and these findings provide preliminary in vitro cellular evidence that formononetin exerts in vitro anti-inflammatory inhibitory activity on TNF-α-activated human RA fibroblast-like synoviocytes. Further in vivo and mechanistic studies are required to explore its potential for anti-RA-related pharmacological application.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"15 17","pages":"2654-2654"},"PeriodicalIF":0.0,"publicationDate":"2026-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148861333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroids promote sugar synthesis by inhibiting BIN2 phosphorylation of phosphoenolpyruvate carboxykinase 油菜素内酯通过抑制磷酸烯醇丙酮酸羧激酶的BIN2磷酸化促进糖的合成。

IF 15 1区生物学

Nature Plants Pub Date : 2026-08-28 DOI: 10.1038/s41477-026-02379-5

Hongliang Zhang, Yalikunjiang Aizezi, Kanako Bessho-Uehara, Ajeet Chaudhary, Cao Son Trinh, Shou-Ling Xu, Zhi-Yong Wang

{"title":"Brassinosteroids promote sugar synthesis by inhibiting BIN2 phosphorylation of phosphoenolpyruvate carboxykinase","authors":"Hongliang Zhang, Yalikunjiang Aizezi, Kanako Bessho-Uehara, Ajeet Chaudhary, Cao Son Trinh, Shou-Ling Xu, Zhi-Yong Wang","doi":"10.1038/s41477-026-02379-5","DOIUrl":"10.1038/s41477-026-02379-5","url":null,"abstract":"Sugar is both an essential energy source and the major substrate for cell wall biosynthesis during plant growth, yet how growth-promoting hormones regulate sugar synthesis remains unclear. Here we show that the brassinosteroids (BRs) promote gluconeogenic and photosynthetic sugar synthesis by activating phosphoenolpyruvate carboxykinase (PCK), which catalyses the conversion of oxaloacetate to phosphoenolpyruvate, a central step in primary metabolism. Arabidopsis BR-deficient mutants display reduced PCK1 activity and elevated phosphorylation at conserved Ser-62 and Thr-66 residues. BR treatment induces PCK1 dephosphorylation and activation, whereas the GSK3-like kinase BIN2 phosphorylates these sites, altering quaternary structure and inhibiting PCK1. Phospho-blocking mutations of Ser-62/Thr-66 confer BR-independent PCK1 activity and enhance seedling growth, while phosphomimetic mutations reduce PCK1 activity and impair seedling growth and establishment. BRs also promote PCK dephosphorylation and activation in photosynthetic leaves of maize and sorghum. Our study demonstrates that BRs regulate primary metabolism via GSK3/BIN2-mediated phosphorylation of PCK, thereby promoting gluconeogenesis and photosynthesis. Whether growth-promoting hormones boost sugar synthesis has remained unclear. Brassinosteroids were found to do so by relieving BIN2-mediated inhibition of PCK1, a key enzyme in gluconeogenesis and photosynthesis.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":"12 9","pages":"1741-1755"},"PeriodicalIF":15.0,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.comhttps://www.nature.com/articles/s41477-026-02379-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148849886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0