Nature Plants最新文献_第5页

Megafire smoke exposure jeopardizes tree carbohydrate reserves and yield 特大火灾的烟雾危害树木的碳水化合物储备和产量

IF 18 1区生物学

Nature Plants Pub Date : 2024-10-02 DOI: 10.1038/s41477-024-01819-4

Jessica Orozco, Paula Guzmán-Delgado, Maciej A. Zwieniecki

引用次数: 0

From RdDM to plant defence 从 RdDM 到植物防御

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-30 DOI: 10.1038/s41477-024-01806-9

Claudia Martinho

引用次数: 0

A novel in vivo genome editing doubled haploid system for Zea mays L. 用于玉米的新型体内基因组编辑加倍单倍体系统

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01795-9

Huaxun Ye, Mei Louden, Jon A. T. Reinders

{"title":"A novel in vivo genome editing doubled haploid system for Zea mays L.","authors":"Huaxun Ye, Mei Louden, Jon A. T. Reinders","doi":"10.1038/s41477-024-01795-9","DOIUrl":"10.1038/s41477-024-01795-9","url":null,"abstract":"Doubled haploid (DH) technologies accelerate maize inbred development. Recently, methods using CRISPR–Cas have created gene-edited maize DH populations, albeit with relatively low editing frequencies. Restoring fertility via haploid chromosome doubling remains a critically important production constraint. Thus, improved editing and chromosome doubling outcomes are needed. Here we obtained maternally derived diploid embryos in vivo by ectopically co-expressing Zea mays BABY BOOM and cyclin D-like gene products within unfertilized egg cells. When combined with gene editing, the in vivo method enables the production of mature seed with a maternally derived, gene-edited diploid embryo without requiring in vitro tissue culture methods nor the use of a chemical chromosome doubling agent. In summary, we report a novel approach for creating gene-edited maize DH populations that we expect can accelerate genetic gain in a scalable, cost-effective manner. Ye et al. use a novel genetic haploid genome doubling method with parthenogenesis and gene editing to produce edited, maternally derived di-haploid progeny. A truncated BABY BOOM peptide confers both parthenogenesis and haploid genome doubling.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide 逆转录转座子驱动的FLC环境调控导致对除草剂的适应性反应

IF 18 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01807-8

Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana

{"title":"Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide","authors":"Mathieu Raingeval, Basile Leduque, Pierre Baduel, Alejandro Edera, Fabrice Roux, Vincent Colot, Leandro Quadrana","doi":"10.1038/s41477-024-01807-8","DOIUrl":"https://doi.org/10.1038/s41477-024-01807-8","url":null,"abstract":"The mobilization of transposable elements is a potent source of mutations. In plants, several stransposable elements respond to external cues, fuelling the hypothesis that natural transposition can create environmentally sensitive alleles for adaptation. Here we report on the detailed characterization of a retrotransposon insertion within the first intron of the Arabidopsis floral-repressor gene FLOWERING LOCUS C (FLC) and the discovery of its role for adaptation. The insertion mutation augments the environmental sensitivity of FLC by affecting the balance between coding and non-coding transcripts in response to stress, thus expediting flowering. This balance is modulated by DNA methylation and orchestrated by IBM2, a factor involved in the processing of intronic heterochromatic sequences. The stress-sensitive allele of FLC has spread across populations subjected to recurrent chemical weeding, and we show that retrotransposon-driven acceleration of the life cycle represents a rapid response to herbicide application. Our work provides a compelling example of a transposable element-driven environmentally sensitive allele that confers an adaptive response in nature.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142329015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposable elements underlie genetic adaptation 转座元件是遗传适应的基础

IF 18 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01792-y

Marco Catoni

引用次数: 0

Random epigenetic inactivation of the X-chromosomal HaMSter gene causes sex ratio distortion in persimmon X 染色体 HaMSter 基因的随机表观遗传失活导致柿树性别比失真

IF 18 1区生物学

Nature Plants Pub Date : 2024-09-27 DOI: 10.1038/s41477-024-01805-w

Takashi Akagi, Shigeo S. Sugano

{"title":"Random epigenetic inactivation of the X-chromosomal HaMSter gene causes sex ratio distortion in persimmon","authors":"Takashi Akagi, Shigeo S. Sugano","doi":"10.1038/s41477-024-01805-w","DOIUrl":"https://doi.org/10.1038/s41477-024-01805-w","url":null,"abstract":"In contrast to the recent progress in the genome sequencing of plant sex chromosomes, the functional contribution of the genes in sex chromosomes remains little known1. They were classically thought to be related to sexual dimorphism, which is beneficial to male or female functions, including segregation ratios. Here we focused on the functional evolution of the sex ratio distortion-related locus Half Male Sterile/Inviable (HaMSter), which is located in the short sex-linked region in diploid persimmon (Diospyros lotus). The expression of HaMSter, encoding a plant1589-like undefined protein, is necessary for production of viable seeds. Notably, only X-allelic HaMSter is substantially expressed and half of the maternal X alleles of HaMSter is randomly inactivated, which results in sex ratio distortion in seeds. Genome-wide DNA methylome analyses revealed endosperm-specific DNA hypermethylation, especially in the X-linked region. The maintenance/release of this hypermethylation is linked to inactivation/activation of HaMSter expression, respectively, which determines the sex ratio distortion pattern.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicide-resistant plants produced by precision adenine base editing in plastid DNA 通过对质体 DNA 进行精确的腺嘌呤碱基编辑培育出抗除草剂植物

IF 18 1区生物学

Nature Plants Pub Date : 2024-09-26 DOI: 10.1038/s41477-024-01808-7

Young Geun Mok, Sunghyun Hong, Da In Seo, Seunghee Choi, Hee Kyoung Kim, Da Mon Jin, JungEun Joanna Lee, Jin-Soo Kim

{"title":"Herbicide-resistant plants produced by precision adenine base editing in plastid DNA","authors":"Young Geun Mok, Sunghyun Hong, Da In Seo, Seunghee Choi, Hee Kyoung Kim, Da Mon Jin, JungEun Joanna Lee, Jin-Soo Kim","doi":"10.1038/s41477-024-01808-7","DOIUrl":"https://doi.org/10.1038/s41477-024-01808-7","url":null,"abstract":"CRISPR-free, protein-only cytosine base editors (CBEs) or adenine base editors, composed of DNA-binding proteins such as zinc finger proteins or transcription activator-like effectors (TALEs) and nucleobase cytosine or adenine deaminases, respectively, enable organellar DNA editing in cultured cells, animals and plants1,2,3,4. TALE-linked double-stranded DNA deaminase toxin A (DddAtox)-derived CBEs (DdCBEs) and TALE-linked adenine deaminases (TALEDs) install C-to-T and A-to-G single-nucleotide conversions, respectively, in mitochondria and chloroplasts5,6,7,8,9. Interestingly, whereas TALEDs exclusively induce A-to-G conversions without C-to-T conversions in mammalian mitochondrial DNA10, they often install unwanted C-to-T edits in addition to intended A-to-G edits in plastid DNA7,9,11,12. Here we show that uracil DNA glycosylase (UDG)-fused TALEDs (UDG-TALEDs) minimize C-to-T conversions without reducing the A-to-G editing efficiency and install a mutation in the chloroplast psbA gene that encodes a single-amino-acid substitution (S264G), which confers herbicide resistance in the resulting plants.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":18.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142321828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of epitranscriptomes uncover translational reprogramming directed by ac4C in rice during pathogen infection 表转录组的动态变化揭示了水稻在病原体感染期间由ac4C引导的翻译重编程过程

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-24 DOI: 10.1038/s41477-024-01800-1

Xiang Lu, Yao He, Jin-Qiao Guo, Yue Wang, Qian Yan, Qing Xiong, Hui Shi, Qingqing Hou, Junjie Yin, Yi-Bang An, Yi-Di Chen, Cheng-Shuang Yang, Ye Mao, Xiaobo Zhu, Yongyan Tang, Jiali Liu, Yu Bi, Li Song, Long Wang, Yihua Yang, Min He, Weitao Li, Xuewei Chen, Jing Wang

{"title":"Dynamics of epitranscriptomes uncover translational reprogramming directed by ac4C in rice during pathogen infection","authors":"Xiang Lu, Yao He, Jin-Qiao Guo, Yue Wang, Qian Yan, Qing Xiong, Hui Shi, Qingqing Hou, Junjie Yin, Yi-Bang An, Yi-Di Chen, Cheng-Shuang Yang, Ye Mao, Xiaobo Zhu, Yongyan Tang, Jiali Liu, Yu Bi, Li Song, Long Wang, Yihua Yang, Min He, Weitao Li, Xuewei Chen, Jing Wang","doi":"10.1038/s41477-024-01800-1","DOIUrl":"10.1038/s41477-024-01800-1","url":null,"abstract":"Messenger RNA modifications play pivotal roles in RNA biology, but comprehensive landscape changes of epitranscriptomes remain largely unknown in plant immune response. Here we report translational reprogramming directed by ac4C mRNA modification upon pathogen challenge. We first investigate the dynamics of translatomes and epitranscriptomes and uncover that the change in ac4C at single-base resolution promotes translational reprogramming upon Magnaporthe oryzae infection. Then by characterizing the specific distributions of m1A, 2’O-Nm, ac4C, m5C, m6A and m7G, we find that ac4Cs, unlike other modifications, are enriched at the 3rd position of codons, which stabilizes the Watson–Crick base pairing. Importantly, we demonstrate that upon pathogen infection, the increased expression of the ac4C writer OsNAT10/OsACYR (N-ACETYLTRANSFERASE FOR CYTIDINE IN RNA) promotes translation to facilitate rapid activation of immune responses, including the enhancement of jasmonic acid biosynthesis. Our study provides an atlas of mRNA modifications and insights into ac4C function in plant immunity. By characterizing the dynamics of epitranscriptomes and translatomes upon Magnaporthe oryzae infection, the authors uncover that ac4C enriched in the 3rd nt of codons improves translation of mRNA to enhance rice resistance against M. oryzae.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142313712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis U1 snRNP regulates mRNA 3′-end processing 拟南芥 U1 snRNP 调控 mRNA 3′端加工

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-23 DOI: 10.1038/s41477-024-01796-8

Anchilie F. Mangilet, Joachim Weber, Sandra Schüler, Manon Adler, Eneza Yoeli Mjema, Paula Heilmann, Angie Herold, Monique Renneberg, Luise Nagel, Irina Droste-Borel, Samuel Streicher, Thomas Schmutzer, Gregor Rot, Boris Macek, Cornelius Schmidtke, Sascha Laubinger

{"title":"The Arabidopsis U1 snRNP regulates mRNA 3′-end processing","authors":"Anchilie F. Mangilet, Joachim Weber, Sandra Schüler, Manon Adler, Eneza Yoeli Mjema, Paula Heilmann, Angie Herold, Monique Renneberg, Luise Nagel, Irina Droste-Borel, Samuel Streicher, Thomas Schmutzer, Gregor Rot, Boris Macek, Cornelius Schmidtke, Sascha Laubinger","doi":"10.1038/s41477-024-01796-8","DOIUrl":"10.1038/s41477-024-01796-8","url":null,"abstract":"The removal of introns by the spliceosome is a key gene regulatory mechanism in eukaryotes, with the U1 snRNP subunit playing a crucial role in the early stages of splicing. Studies in metazoans show that the U1 snRNP also conducts splicing-independent functions, but the lack of genetic tools and knowledge about U1 snRNP-associated proteins have limited the study of such splicing-independent functions in plants. Here we describe an RNA-centric approach that identified more than 200 proteins associated with the Arabidopsis U1 snRNP and revealed a tight link to mRNA cleavage and polyadenylation factors. Interestingly, we found that the U1 snRNP protects mRNAs against premature cleavage and polyadenylation within introns—a mechanism known as telescripting in metazoans—while also influencing alternative polyadenylation site selection in 3′-UTRs. Overall, our work provides a comprehensive view of U1 snRNP interactors and reveals novel functions in regulating mRNA 3′-end processing in Arabidopsis, laying the groundwork for understanding non-canonical functions of plant U1 snRNPs. Researchers found that a plant U1 snRNP complex associates with 200 proteins and conducts splicing-independent roles by safeguarding RNAs against premature cleavage and polyadenylation, similar to a process known as telescripting in metazoans.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41477-024-01796-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142276827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chloroplast elongation factors break the growth–immunity trade-off by simultaneously promoting yield and defence 叶绿体伸长因子同时促进产量和防御，打破了生长与免疫之间的平衡

IF 15.8 1区生物学

Nature Plants Pub Date : 2024-09-19 DOI: 10.1038/s41477-024-01793-x

Yetong Qi, Jiahui Wu, Zhu Yang, Hongjun Li, Lang Liu, Haixia Wang, Xinyuan Sun, Xinya Wu, Jiahui Nie, Jing Zhou, Meng Xu, Xintong Wu, Susan Breen, Ruimin Yu, Dong Cheng, Qingguo Sun, Huishan Qiu, Yingtao Zuo, Petra C. Boevink, Paul R. J. Birch, Zhendong Tian

{"title":"Chloroplast elongation factors break the growth–immunity trade-off by simultaneously promoting yield and defence","authors":"Yetong Qi, Jiahui Wu, Zhu Yang, Hongjun Li, Lang Liu, Haixia Wang, Xinyuan Sun, Xinya Wu, Jiahui Nie, Jing Zhou, Meng Xu, Xintong Wu, Susan Breen, Ruimin Yu, Dong Cheng, Qingguo Sun, Huishan Qiu, Yingtao Zuo, Petra C. Boevink, Paul R. J. Birch, Zhendong Tian","doi":"10.1038/s41477-024-01793-x","DOIUrl":"10.1038/s41477-024-01793-x","url":null,"abstract":"Chloroplasts regulate plant development and immunity. Here we report that potato chloroplast elongation factors StTuA and StTuB, targeted by Phytophthora infestans RXLR effector Pi22926, positively regulate immunity and growth. Plants expressing Pi22926, or silenced for TuA/B, show increased P. infestans susceptibility and decreased photosynthesis, plant growth and tuber yield. By contrast, StTuA/B overexpression reduces susceptibility, elevates chloroplast-derived reactive oxygen species production and increases photosynthesis and potato tuber yield by enhancing chloroplast protein translation. Another plant target of Pi22926, StMAP3Kβ2, interacts with StTuB, phosphorylating it to promote its translocation into chloroplasts. However, Pi22926 attenuates StTuB association with StMAP3Kβ2 and phosphorylation. This reduces StTuB translocation into chloroplasts, leading to its proteasome-mediated turnover in the cytoplasm. We uncover new mechanisms by which a pathogen effector inhibits immunity by disrupting key chloroplast functions. This work shows that StTuA/B break the growth–immunity trade-off, promoting both disease resistance and yield, revealing the enormous potential of chloroplast biology in crop breeding. Enhanced expression of chloroplast elongation factors StTuA/B increases potato disease resistance and photosynthesis, while oomycete effector Pi22926 inhibits their entry into the chloroplasts by disrupting their phosphorylation by StMAP3Kβ2.","PeriodicalId":18904,"journal":{"name":"Nature Plants","volume":null,"pages":null},"PeriodicalIF":15.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142245257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0