Nanotechnology, Science and Applications最新文献

{"title":"Biogenic Nickel Ferrite Nanoparticles Synthesized From Agro-Waste: Green Synthesis, Physicochemical Characterization, and Potential for Magnetic Hyperthermia Applications.","authors":"Małgorzata Góral-Kowalczyk, Elżbieta Grządka, Jolanta Orzeł, Dariusz Góral, Tomasz Skrzypek, Zbigniew Kobus, Iryna Koval, Andrzej Marczuk","doi":"10.2147/NSA.S628375","DOIUrl":"10.2147/NSA.S628375","url":null,"abstract":"<p><strong>Introduction: </strong>Nickel ferrite (NiFe₂O₄) nanoparticles are promising magnetic hyperthermia agents, but conventional synthesis relies on toxic reagents. Plant-extract-mediated biogenic synthesis offers an eco-friendly alternative; however, the influence of specific agro-waste phytochemical matrices and precursor chemistry on nanoparticle structure and heating efficiency remains poorly characterized.</p><p><strong>Methods: </strong>Aqueous extracts from pomegranate peel (Punica granatum L) and raspberry leaves/shoots (Rubus idaeus L) were characterized for total phenolic content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity. Cherry stalk extract showed insufficient reactivity and was excluded. Two precursor systems - nitrate (Fe(NO₃)₃/Ni(NO₃)₂) and acetate-sulfate (Ni(CH₃COO)₂/FeSO₄) - were combined with each extract, yielding four nanoparticle batches (M1, M2, G1, G2) after calcination at 500°C for 4 h. Nanoparticles were characterized by XRD, XRF, FTIR, TEM, DLS, and zeta potential. Magnetic heating performance was assessed under AMF (532.4 kHz, 3.1 kA·m^-1); SAR and ILP were calculated with ferrite-mass correction based on XRF data.</p><p><strong>Results: </strong>Pomegranate extract showed the highest phytochemical activity (DPPH: 1283.9 μM TE·g^-1). XRD confirmed the spinel NiFe₂O₄ structure in all batches, with crystallite sizes of 15.0-21.8 nm and lattice parameters of 8.35-8.38 Å. The acetate-sulfate batches (M2, G2) contained substantially higher Fe/Ni fractions (M2: Fe 49.0%, Ni 11.5%) than the nitrate batches (M1, G1: Fe ≤2.9%, Ni ≤1.7%). DLS indicated hydrodynamic diameters of 200-400 nm; zeta potential values (-14.0 to +7.8 mV) indicated limited colloidal stability. Under AMF, M2 and G2 reached maximum temperatures of 46.0°C and 43.4°C (baseline 31°C), with SAR of 15.7 and 15.0 W·g^-1 and ILP of 3.08 and 2.93 nH·m²·kg^-1, respectively. Sample M1 showed negligible heating (SAR 0.93 W·g^-1; ΔT =1.1°C).</p><p><strong>Discussion: </strong>Precursor chemistry critically determines ferrite yield and heating efficiency, with the acetate-sulfate system consistently outperforming the nitrate system. The obtained ILP values are comparable to literature reports for NiFe₂O₄ under similar AMF conditions. However, the observed aggregation and low zeta potential limit biomedical applicability. As no cytotoxicity or in vitro/in vivo data were obtained, these findings represent proof-of-concept for the thermal conversion potential of biogenically synthesized NiFe₂O₄, pending biological validation.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"628375"},"PeriodicalIF":7.6,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13450626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanofiber Matrix Dressing for Split-Thickness Skin Graft Donor Sites: A Randomized Comparative Clinical Study.","authors":"Maciej Biskupski, Sergey Antonov, Zofia Małgorzata Górecka, Rafał Wójcik, Katarzyna Żuchowska, Marek Majewski, Kamil Torres","doi":"10.2147/NSA.S616791","DOIUrl":"10.2147/NSA.S616791","url":null,"abstract":"<p><strong>Background: </strong>Donor-site morbidity after split-thickness skin graft (STSG) harvest remains a significant clinical concern, with pain representing one of the most burdensome early postoperative symptoms. Electrospun nanofiber matrices have emerged as a promising wound management strategy, yet clinical evidence supporting their use in STSG donor sites remains limited. We evaluated the clinical performance of a nanofiber matrix for donor-site management after STSG harvest.</p><p><strong>Methods: </strong>This single-center randomized comparative study enrolled 40 adult patients undergoing STSG harvest into either the nanofiber matrix group (n=20) or the conventional dressing group with chlorhexidine paraffin gauze and povidone-iodine gauze (n=20). Pain intensity was measured on a 0-10 scale on postoperative days 1, 2, 5, and 14. Secondary outcomes included donor-site healing time, pruritus during healing, late scar hyperpigmentation, scar elevation, late pruritus, and local or systemic signs of infection.</p><p><strong>Results: </strong>Compared with conventional management, the nanofiber matrix significantly reduced donor-site pain on postoperative day 1 (p<0.001), day 2 (p<0.001), and day 5 (p=0.015), but not on day 14 (p=0.36). The nanofiber group also healed faster than the control group (15.05±2.34 vs 18.15±4.38 days; p=0.045). Pruritus during healing did not differ significantly between groups (p=0.97). At late follow-up, we found no significant between-group differences in hyperpigmentation (p=0.11), scar elevation (p=0.051), or late pruritus (p=1.0). Mild exudate occurred in 3 patients in the nanofiber group, without a significant between-group difference (p=0.12), and we identified no other infection-related complications.</p><p><strong>Conclusion: </strong>Nanofiber matrix dressing reduced early postoperative pain and shortened healing time at STSG donor sites compared with conventional dressing, while late scar outcomes and short-term safety remained comparable between groups. These findings support the potential of nanofiber-based donor-site management as a clinically relevant translational application of electrospinning technology.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"616791"},"PeriodicalIF":7.6,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silver Nanoparticles Attenuate Histamine-Induced Pruritus via Modulation of the Nitric Oxide/iNOS Signaling Pathway in Mice.","authors":"Abdul Mobin Bayan, Muhammad Imran Khan","doi":"10.2147/NSA.S591039","DOIUrl":"10.2147/NSA.S591039","url":null,"abstract":"<p><strong>Background: </strong>Pruritus is an unpleasant cutaneous sensation that provokes scratching behavior and is associated with numerous inflammatory and dermatological disorders. Histamine is a major mediator of pruritus and contributes to the activation of inflammatory and oxidative stress pathways. Silver nanoparticles (Ag-NPs) possess well-documented anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. The present study investigated the antipruritic effects of Ag-NPs and the involvement of the nitric oxide (NO) signaling pathway in histamine-induced pruritus in mice.</p><p><strong>Methods: </strong>Silver nanoparticles were synthesized using a green synthesis approach and characterized by UV-Vis spectroscopy, FTIR, and SEM analyses. Histamine-induced pruritus was established in male NMRI mice by intradermal injection of histamine (400 µg) into the shaved nape of the neck. Ag-NPs were administered intraperitoneally at doses of 1, 1.5, and 2 mg/kg. To investigate the role of nitric oxide signaling, mice received the nitric oxide synthase inhibitors Nω-Nitro-L-arginine methyl ester (L-NAME, 1 mg/kg) or aminoguanidine (AG, 200 mg/kg) prior to histamine administration. Scratching behavior was recorded, while oxidative stress markers, nitric oxide levels, nerve growth factor (NGF), inducible nitric oxide synthase (iNOS) expression, and histopathological changes were evaluated.</p><p><strong>Results: </strong>Histamine administration increased scratching behavior, nitric oxide production, nerve growth factor (NGF) levels, oxidative stress, and iNOS gene expression in mouse skin tissue. Treatment with Ag-NPs significantly reduced histamine-induced scratching and attenuated nitric oxide production. Ag-NPs also improved antioxidant status by increasing glutathione, glutathione S-transferase, and catalase levels while reducing lipid peroxidation. Furthermore, Ag-NPs treatment significantly decreased NGF levels and suppressed iNOS mRNA expression. Histopathological examination revealed marked improvement in skin architecture and reduced tissue damage in Ag-NP-treated groups. The antipruritic effects of Ag-NPs were further enhanced in combination with nitric oxide synthase inhibitors, suggesting the involvement of the NO/iNOS signaling pathway.</p><p><strong>Conclusion: </strong>Silver nanoparticles exhibit antipruritic activity against histamine-induced pruritus in mice. Their protective effects are associated with suppression of the NO/iNOS pathway, reduction of oxidative stress, modulation of NGF expression, and attenuation of inflammatory responses. These findings suggest that Ag-NPs may represent a promising therapeutic strategy for the management of pruritic disorders.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"591039"},"PeriodicalIF":7.6,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13340303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three Noble Metal Nanoparticles Demonstrated Distinct Biological Interactions and Cell Death Pathways in Human Hepatocarcinoma Cell Lines.","authors":"Kacper Wiktorowski, Kamila Podsiadło, Nasrin Abbasi Gharibkandi, Sylwia Michlewska, Katarzyna D Kania, Aleksander Bilewicz, Agnieszka Majkowska-Pilip, Marzena Szwed","doi":"10.2147/NSA.S589907","DOIUrl":"10.2147/NSA.S589907","url":null,"abstract":"<p><strong>Purpose: </strong>New, more effective therapies are needed for patients with liver tumors who are ineligible for surgical resection, transplantation, or local ablation. Noble metal nanoparticles (NPs) exhibit substantial biological reactivity toward liver cancer cells, yet their unpredictable nanotoxicity highlights the need for a deeper understanding of NP-cell interactions. The present study examined the in vitro toxicological properties of gold (Au), palladium (Pd), and Pd-Au NPs in three hepatocellular carcinoma cell lines (HepG2, Hep3B, and Huh7D-12).</p><p><strong>Methods: </strong>The cytotoxicity of noble-metal-based NPs was assessed using Alamar Blue and MTT colorimetric assays. Reactive oxygen species generation and oxidative stress markers were quantified by fluorometric, luminometric, and flow cytometry methods. Confocal microscopy combined with fluorescence readouts were used to evaluate mitochondrial homeostasis and programmed cell death pathways. Molecular markers of NP-induced cellular stress were further confirmed using qRT-PCR.</p><p><strong>Results: </strong>Distinct cellular differences were observed among the three NPs, particularly in terms of cytotoxicity and death modalities. Au NPs caused mild mitochondrial perturbations without significant cytotoxicity. Pd NPs induced necroptosis, primarily at high concentrations. In contrast, Pd-Au NPs induced oxidative stress, lipid peroxidation, glutathione depletion, and transcriptional changes in the genes regulating glutathione synthesis and metabolism. Pd-Au NPs also activated ferroptosis, which is a regulated cell death pathway increasingly recognized as a promising anticancer strategy.</p><p><strong>Conclusion: </strong>Among the tested formulations, Pd-Au NPs exhibited the strongest cytotoxic and antiproliferative effects that were driven by mitochondrial disruption and ferroptosis induction. These in vitro findings provided a mechanistic reason for advancing Pd-Au NP research efforts into in vivo studies and patient-derived preclinical models for hepatocellular carcinoma.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"589907"},"PeriodicalIF":7.6,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13242778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parenteral Berberine vs Cisplatin- Loaded Lipid Nanoparticles - Development, Characterisation, Comparative Safety Profiling and Cytotoxicity in Cholangiocarcinoma Cell Models.","authors":"Martin Manov, Denitsa Stefanova, Virginia Tzankova, Borislav Tzankov, Elisa Lozano, Kevin Delgado-Calvo, Rocio I R Macias, Jose J G Marin, Ivanka Spassova, Daniela Kovacheva, Marta Slavkova","doi":"10.2147/NSA.S596038","DOIUrl":"10.2147/NSA.S596038","url":null,"abstract":"<p><strong>Introduction: </strong>Cholangiocarcinoma (CCA) is an aggressive and heterogeneous malignancy of the biliary tract with a poor prognosis. Berberine can be a therapeutic option with low toxicity, but its delivery remains challenging. Encapsulation in lipid nanoparticles offers a promising biocompatible strategy to improve the delivery and safety of berberine.</p><p><strong>Purpose: </strong>This study aimed to develop, characterize berberine-loaded nanostructured lipid carriers (N-Ber) and evaluate their cytotoxicity in CCA cell models in comparison to a standard chemotherapeutic, cisplatin, in both free and nanoparticle-loaded form (N-Cis). The in vitro biocompatibility for potential parenteral delivery of the nanocarriers was a secondary goal of the work.</p><p><strong>Methods: </strong>Nanoparticles were prepared by solvent evaporation with Precirol 5 ATO and oleic acid as lipids and Tween 20 as a stabilizer and were characterized by DLS, morphology, encapsulation efficiency, and in vitro drug release at physiological (pH 7.4) and tumor-mimicking (pH 5.5) conditions. Cytotoxicity was evaluated in three CCA cell lines (TFK-1, EGI-1, and HuCCT1) using the MTT assay, while endothelial cells (Ea.hy926) and the hemolysis test in human erythrocytes were employed to evaluate safety.</p><p><strong>Results: </strong>N-Ber and N-Cis displayed mean sizes 159.5 nm and 146.7 nm, respectively, negative surface charge (-27.95 mV and -50.75 mV), and high encapsulation efficiencies (berberine: 88.8%; cisplatin: 95.8%). Both nanoformulations showed significantly altered dissolution profiles compared to the free drugs. Berberine showed potent cytotoxicity against all CCA cell lines (IC₅ ₀ 0.4-5.2 µM), comparable or superior to cisplatin (IC₅ ₀ ≈ 10 µM) and these effects were preserved after nanoencapsulation. In contrast, N-Ber exhibited low toxicity towards endothelial cells and no relevant hemolytic activity (<5% hemolysis) at therapeutic concentrations, while N-Cis induced marked hemolysis at all tested doses.</p><p><strong>Discussion: </strong>N-Ber combines strong cytotoxicity in CCA cells with a favorable biocompatibility compared to N-Cis. The promising in vitro data warrant further in vivo evaluation.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"596038"},"PeriodicalIF":7.6,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13200210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148033039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sacha Inchi Oil-Based Nanostructured Lipid Carriers for Curcumin Delivery: Development and Physicochemical Characterization.","authors":"Ikra Nurohman, Anis Yohana Chaerunisaa, Gofarana Wilar, Garnadi Jafar, Cecep Suhandi, Sriwidodo Sriwidodo","doi":"10.2147/NSA.S589429","DOIUrl":"10.2147/NSA.S589429","url":null,"abstract":"<p><strong>Background and objective: </strong>Curcumin exhibits potent antioxidant activity beneficial for the prevention of various degenerative diseases; however, its highly lipophilic nature and susceptibility to degradation limit its solubility and physicochemical stability. To address these limitations, curcumin was formulated into nanostructured lipid carriers (NLCs), a lipid-based colloidal delivery system composed of solid and liquid lipids stabilized by surfactants. In this study, sacha inchi oil, a natural oil rich in omega-3 and other unsaturated fatty acids, was investigated as a novel liquid lipid component to improve lipid matrix structure and drug accommodation.</p><p><strong>Methods: </strong>Curcumin-loaded NLCs were prepared using the hot homogenization method followed by probe sonication. Curcumin served as the active compound, while solid lipids (oleum cacao, glyceryl behenate (Compritol^® 888 ATO), or glyceryl palmitostearate (Precirol^® ATO 5)), sacha inchi oil as the liquid lipid, and surfactants (Tween 80, Poloxamer, or a Tween 80-Span 80 combination) were used. The resulting NLCs were characterized in terms of particle size, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency (EE), physicochemical properties (FTIR, DSC, XRD), morphology, and in vitro release behavior.</p><p><strong>Results: </strong>Physicochemical analyses confirmed successful incorporation of curcumin into the lipid matrix without undesirable interactions. Among the tested formulations, CaTS2 (oleum cacao 4.5%, sacha inchi oil 1%, Tween 80 12.5%, Span 80 1%, and curcumin 0.1%) demonstrated the most favorable characteristics, with a particle size of 95.50 ± 0.87 nm, PDI of 0.119 ± 0.157, and ZP of -22.30 ± 0.98 mV. Entrapment efficiency reached 97.24% and morphological analysis showed predominantly spherical particles. In vitro release exhibited a biphasic pattern, consisting of an initial burst followed by sustained release up to 480 min. Kinetic modeling revealed that CaTS2 followed the Korsmeyer-Peppas model (<i>R²</i> = 0.793; n = 0.301), consistent with Fickian diffusion, whereas pure curcumin followed the Higuchi model (<i>R²</i> = 0.819). The similarity factor (f₂ = 29.04) indicated a distinctly different release profile between the two systems.</p><p><strong>Conclusion: </strong>Sacha inchi oil-based nanostructured lipid carriers were successfully developed and demonstrated favorable physicochemical characteristics, supporting their potential as a stable delivery system for curcumin.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"589429"},"PeriodicalIF":7.6,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13094562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cost-Effective Approach to Triphenylamine-Assisted Fabrication of Stable Cu₂O Nanoparticles: Structural Analysis and Multifunctional Antibacterial, Optical, and Electronic Performance.","authors":"Omid Azizian-Shermeh, Ali Reza Modarresi-Alam, Ebrahim Mollashahi, Sahar Shabzendedar","doi":"10.2147/NSA.S571946","DOIUrl":"10.2147/NSA.S571946","url":null,"abstract":"<p><strong>Introduction: </strong>Research interest in nanomaterials has surged because of their unique physical and chemical characteristics that differentiate them from their bulk counterparts, such as electrical resistivity, strength and hardness, chemical reactivity, optical and electronic properties and a wide range of adaptable biological activity. The primary objective of this study was to develop a facile and cost-effective method for the triphenylamine-assisted synthesis of stable copper (I) oxide (Cu₂O) nanoparticles (NPs) and to comprehensively evaluate their potential optical, electronic, and antibacterial applications. This present study is the first report of a facile and effective method to triphenylamine-assisted synthesis of stable copper (I) oxide (Cu₂O) nanoparticles (NPs).</p><p><strong>Methods: </strong>After triphenylamine-assisted synthesis of Cu₂O NPs, the synthesized NPs were comprehensively characterized through X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), UV-Vis spectroscopy, Fourier-transform infra-red spectroscopy (FTIR), atomic force microscopy (AFM), and thermal gravimetric analysis (TGA). The antimicrobial activity of the Cu₂O NPs was evaluated using the disk diffusion method and determination of minimum inhibitory concentration (MIC) against four clinically significant bacterial strains.</p><p><strong>Results: </strong>XRD analysis confirmed the crystalline cubic structure of Cu₂O NPs, while TEM and SEM revealed spherical morphology with an average particle size of 10-60 nm with the highest frequency of 30 nm in diameter. Optical, electronic and antimicrobial properties of Cu₂O NPs were also studied. UV-Vis spectra exhibited a distinct absorption peak at 275 nm and 280 nm in formic acid and N-methyl pyrrolidone (NMP) solvents, respectively. Electronic properties were investigated using cyclic voltammetry (CV) analyses and electron transitions (direct and indirect) in UV-Vis. Results of antibacterial activities indicated dose-dependent inhibition. The synthesized NPs showed significant efficacy, particularly against Gram-positive bacteria.</p><p><strong>Conclusion: </strong>These findings highlight the potential of stable Cu₂O NPs as durable antimicrobial agents for biomedical and its electrical and optical characteristics making it appropriate for various uses in photovoltaics, sensors, and photocatalysis and industrial applications, offering enhanced longevity and effectiveness compared to conventional counterparts.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"571946"},"PeriodicalIF":7.6,"publicationDate":"2026-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13082260/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Labeling of Amine-Modified Material Surfaces with PFB-Fluorescein.","authors":"Kaja Jaskot, Angelika Maria Mielcarek, Łukasz Popenda, Ahmet Kertmen, Olena Ivashchenko, Bartosz F Grześkowiak, Agnieszka Fedoruk-Wyszomirska, Kamilla Bąkowska-Żywicka, Agata Tyczewska, Jan Barciszewski, Patrick M Perrigue","doi":"10.2147/NSA.S576032","DOIUrl":"10.2147/NSA.S576032","url":null,"abstract":"<p><strong>Introduction: </strong>Fluorescent labeling is a widely used method for visualizing and tracking materials. 5-(Pentafluorobenzoylamino) fluorescein (PFB-F) is a dye in which the pentafluorobenzoylamino group substituent functions as a reactive linker. While PFB-F has been used for labeling biomolecules, its application in labeling surfaces has not been explored yet. Here, we report straightforward method for attaching PFB-F to amine-modified material surfaces requiring only dimethyl sulfoxide (DMSO) solvent and room temperature (RT).</p><p><strong>Methods: </strong>We conducted nuclear magnetic resonance (NMR) and thin layer chromatography (TLC) analysis on a mock reaction involving PFB-F and <i>N</i>-butylamine to evaluate the process of attachment via the PFB linker. PFB-F was also reacted with poly-L-lysine to evaluate conjugation to polymers bearing primary amine groups. Silica nanoparticles (SiNPs) labeled with PFB-F were administered to nematode worms, followed by confocal fluorescence microscopy.</p><p><strong>Results: </strong>NMR and TLC analysis confirmed covalent bonding between PFB-F and primary amines via nucleophilic aromatic substitution (S_NAr) involving displacement of at least one fluorine atom. PFB-F was successfully conjugated to poly-L-lysine, demonstrating efficient reaction with polymers containing primary amine groups. SiNPs labeled with PFB-F were ingested by nematode worms and successfully tracked in vivo.</p><p><strong>Discussion: </strong>These results demonstrate that PFB-F is a versatile and effective reagent for stable surface labeling. This study paves the way for broader applications of PFB-F in material design.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"576032"},"PeriodicalIF":7.6,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-Dimensional MXenes as Next-Generation Nanomaterials for Biosensing and Hydrogen Production.","authors":"Anupam Singha Roy, Neelesh Babu, Aabid Hussain","doi":"10.2147/NSA.S580785","DOIUrl":"10.2147/NSA.S580785","url":null,"abstract":"<p><p>The discovery of graphene, which has led to further research on other two-dimensional (2D) materials, has greatly enhanced the development of sustainable novel materials in the age of nanotechnology. The majority of elements in the periodic table are currently converted into 2D forms by researchers. Materials such as graphene and its derivatives, transition-metal dichalcogenides (TMDs), and transition-metal carbides (MXenes) have been extensively used because of their exceptional electronic and optical properties. While addressing synthesis challenges and stability issues, functionalization is one of the strategies used to overcome the difficulties related to the stability and large dimensions of 2D materials. This review provides detailed studies on MXene synthesis methods and their characteristic properties, emphasizing the importance of modifying MXenes for biosensing applications such as the detection of pathogenic viruses and bacteria, mycotoxins, hazardous pollutants, food contaminants, biomolecules, and cancer biomarkers. A review of the function of MXenes in hydrogen production highlights how well they improve charge transfer and lower reaction overpotentials. The future prospects of MXene-based biosensors as advanced diagnostic tools and hydrogen catalysts are also discussed, in addition to surface functionalization engineering and hybridization techniques.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"580785"},"PeriodicalIF":7.6,"publicationDate":"2026-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12991872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles as a Promising Approach for Improving Skin Anti-Aging Activity.","authors":"Yolanda Putri Aloenida, Mayang Kusuma Dewi, Muhaimin Muhaimin, Anis Yohana Chaerunisaa","doi":"10.2147/NSA.S571010","DOIUrl":"10.2147/NSA.S571010","url":null,"abstract":"<p><p>The aging of skin is a multifaceted process influenced by internal factors and external influences. These factors contribute to the breakdown of skin structure, diminished skin resilience, and observable indications of aging. Conventional topical formulations often fail to deliver active chemicals adequately due to the skin's inherent barriers and the solubility and instability of numerous substances. Emerging nanotechnology-based technologies offer a viable solution to these restrictions. This paper analyzes the role of diverse organic and inorganic nanoparticle-based formulations as delivery systems in improving the distribution and efficacy of anti-aging agents. As part of its novelty, this article integrates findings on both synthetic and natural anti-aging compounds, providing a comprehensive comparison of their nano-enabled enhancements findings from in vitro and in vivo models, along with clinical studies. Comparative studies consistently demonstrate that nano-formulations surpass conventional approaches in enhancing antioxidant defense, stimulating collagen synthesis, and suppressing enzymes associated with skin aging.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"19 ","pages":"571010"},"PeriodicalIF":7.6,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12989699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}