Nanotechnology, Science and Applications最新文献

{"title":"Nanoparticles in Plant Cryopreservation: Effects on Genetic Stability, Metabolic Profiles, and Structural Integrity in Bleeding Heart (Papaveraceae) Cultivars.","authors":"Dariusz Kulus, Alicja Tymoszuk, Alicja Kulpińska, Bożena Dębska, Agata Michalska, Julita Nowakowska, Dorota Wichrowska, Jacek Wojnarowicz, Urszula Szałaj","doi":"10.2147/NSA.S485428","DOIUrl":"10.2147/NSA.S485428","url":null,"abstract":"<p><strong>Purpose: </strong>Studying the role of nanoparticles in plant cryopreservation is essential for developing innovative methods to conserve plant genetic resources amid environmental challenges. This research investigated the effects of gold (AuNPs), silver (AgNPs), and zinc oxide (ZnONPs) nanoparticles on the structural integrity, genetic stability, and metabolic activity of cryopreserved plant materials with medicinal properties.</p><p><strong>Methods: </strong>Shoot tips from two bleeding heart (<i>Lamprocapnos spectabilis</i> (L). Fukuhara) cultivars, 'Gold Heart' and 'Valentine', were cryopreserved using the encapsulation-vitrification technique, with nanoparticles added at concentrations of 5 or 15 ppm during either the preculture phase or the alginate bead matrix formation. Post-recovery, the plants underwent histological, molecular, and biochemical analyses.</p><p><strong>Results: </strong>Electron microscopy observations of LN-derived plant material confirmed the production of micro-morpho-structurally stable cells. It was found that nanoparticles could penetrate the cell and accumulate in its various compartments, including the nucleus. As for the genetic analysis, SCoT markers identified polymorphisms in 11.5% of 'Gold Heart' plants, while RAPDs detected mutations in 1.9% of 'Valentine' specimens. Analysis of Molecular Variance (AMOVA) indicated that in the 'Valentine' cultivar, all genetic variation detected was within populations and not significantly affected by nanoparticle treatments. In 'Gold Heart', the majority (94%) of genetic variation detected was within populations, while 6% was attributed to nanoparticle treatments (mostly the application of 15 ppm ZnONPs). The application of nanoparticles significantly influenced the metabolic profile of bleeding heart plants, particularly affecting the synthesis of phenolic acids and aldehydes, as well as the antioxidant mechanisms in both 'Gold Heart' and 'Valentine' cultivars. The content of proteins was altered in 'Gold Heart' plants but not in 'Valentine'.</p><p><strong>Conclusion: </strong>The results suggest that different types and concentrations of NPs have varying effects on the production of specific metabolites, which could be harnessed to modulate plant secondary metabolism for desired pharmacological outcomes.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"35-56"},"PeriodicalIF":4.9,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11844321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consequences of Dietary Manganese-Based Nanoparticles Supplementation or Deficiency on Systemic Health and Gut Metabolic Dynamics in Rats.","authors":"Przemysław Sołek, Karolina Różaniecka, Jerzy Juśkiewicz, Bartosz Fotschki, Anna Stępniowska, Katarzyna Ognik","doi":"10.2147/NSA.S494533","DOIUrl":"10.2147/NSA.S494533","url":null,"abstract":"<p><strong>Introduction: </strong>Trace elements such as manganese (Mn) are essential for various biological processes, including enzyme activation, metabolic pathways, and antioxidant defences. Given its involvement in these critical processes, maintaining adequate Mn levels is crucial for overall health.</p><p><strong>Methods: </strong>The experimental design involved 24 male Wistar rats divided into three groups (n=8 per group): a control group receiving standard Mn supplementation (65 mg/kg), an Mn-deficient group, and a group supplemented with Mn₂O₃ nanoparticles (65 mg/kg). The 12-week feeding trial assessed selected physiological parameters, tissue composition, caecal health, and biochemical markers.</p><p><strong>Results: </strong>Body and major organ weights were not significantly affected across groups (p=0.083 to p=0.579). However, significant differences were observed in fat tissue percentage (p=0.016) and lean tissue percentage (p<0.001). Caecal parameters showed higher ammonia levels (p=0.030) and increased pH (p=0.031) in the nano-Mn group. In turn, total SCFA concentrations were highest in the control group, followed by the Mn-deficient and nano-Mn groups (p<0.001). Enzymatic activities of caecal bacteria differed significantly between the groups, with reduced activity in the nano-Mn group (p<0.001). Blood plasma analysis revealed significantly lower insulin (p<0.001) and neurotransmitter levels, including dopamine and serotonin, in the Mn-deficient and nano-Mn groups compared to controls.</p><p><strong>Discussion: </strong>Our findings suggest that both Mn supplementation and deficiency can lead to physiological and biochemical alterations, affecting fat metabolism, gut health and microbial enzymatic activity or neurotransmitter levels highlighting the critical role of Mn in maintaining metabolic homeostasis or its potential implications for nutritional and pharmaceutical interventions.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"19-34"},"PeriodicalIF":4.9,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11840336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Substantial Role of Cell and Nanoparticle Surface Properties in the Antibacterial Potential of Spherical Silver Nanoparticles [Response to Letter].","authors":"Marta Krychowiak-Maśnicka, Weronika Paulina Wojciechowska, Karolina Bogaj, Aleksandra Bielicka-Giełdoń, Ewa Czechowska, Magdalena Ziąbka, Magdalena Narajczyk, Anna Kawiak, Tomasz Mazur, Beata Szafranek, Aleksandra Królicka","doi":"10.2147/NSA.S518260","DOIUrl":"10.2147/NSA.S518260","url":null,"abstract":"","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"17-18"},"PeriodicalIF":4.9,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Substantial Role of Cell and NanoparticleSurface Properties in the Antibacterial Potential of Spherical Silver Nanoparticles [Letter].","authors":"Danfeng Shen","doi":"10.2147/NSA.S514191","DOIUrl":"10.2147/NSA.S514191","url":null,"abstract":"","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"15-16"},"PeriodicalIF":4.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11759579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Alpha Mangostin Loaded-Mesoporous Silica Nanoparticle and the Impact on Dissolution and Physical Stability.","authors":"Diah Lia Aulifa, Annisa Hafizhah Saepudin, Priskila Margaretha, Miski Aghnia Khairinisa, Arif Budiman","doi":"10.2147/NSA.S499007","DOIUrl":"10.2147/NSA.S499007","url":null,"abstract":"<p><strong>Purpose: </strong>Improving drug solubility is crucial in formulating poorly water-soluble drugs, especially for oral administration. The incorporation of drugs into mesoporous silica nanoparticles (MSN) is widely used in the pharmaceutical industry to improve physical stability and solubility. Therefore, this study aimed to elucidate the mechanism of poorly water-soluble drugs within MSN, as well as evaluate the impact on the dissolution and physical stability.</p><p><strong>Methods: </strong>Alpha mangostin (AM) was adopted as a model of a poorly water-soluble drug, while MSN with the pore size of 45 Å (MSN45) and 120 Å (MSN120) were used as Mesoporous materials. AM-loaded MSN (AM/MSN45 and AM/MSN120) was prepared by solvent evaporation method.</p><p><strong>Results: </strong>The amorphization of AM/MSN45 and AM/MSN120 was confirmed by the halo pattern observed in the powder X-ray diffraction pattern and the absence of the melting peak and the glass transition of AM in the DSC curves. This signified the successful incorporation of AM into MSN. FT-IR measurements suggested the formation of hydrogen bond interaction between the carbonyl group of AM and the silica surface of MSN. In the dissolution test, the presence of the AM within MSN improved the dissolution rate and generated the supersaturation of AM. However, the difference of pores size of MSN could affect the dissolution profile of AM within MSN. Additionally, it retained the X-ray halo patterns after 30 d of storage at 25 ^oC and 0% RH.</p><p><strong>Conclusion: </strong>In conclusion, AM-loaded mesoporous silica significantly improved the dissolution and physical stability.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"1-13"},"PeriodicalIF":4.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727328/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaired Biofilm Development on Graphene Oxide-Metal Nanoparticle Composites.","authors":"Agata Lange, Marta Kutwin, Katarzyna Zawadzka, Agnieszka Ostrowska, Barbara Strojny-Cieślak, Barbara Nasiłowska, Aneta Bombalska, Sławomir Jaworski","doi":"10.2147/NSA.S485841","DOIUrl":"10.2147/NSA.S485841","url":null,"abstract":"<p><strong>Purpose: </strong>Biofilms are one of the main threats related to bacteria. Owing to their complex structure, in which bacteria are embedded in the extracellular matrix, they are extremely challenging to eradicate, especially since they can inhabit both biotic and abiotic surfaces. This study aimed to create an effective antibiofilm nanofilm based on graphene oxide-metal nanoparticles (GOM-NPs).</p><p><strong>Methods: </strong>To create nanofilms, physicochemical analysis was performed, including zeta potential (Zp) (and the nanocomposites stability in time) and size distribution measurements, scanning transmission electron microscopy (STEM), energy dispersive X-ray analysis (EDX), and atomic force microscopy (AFM) of the nanofilm surfaces. During biological analysis, reactive oxygen species (ROS) and antioxidant capacity were measured in planktonic cells treated with the nanocomposites. Thereafter, biofilm formation was checked via crystal violet staining, biofilm thickness was assessed by confocal microscopy using double fluorescent staining, and biofilm structure was analyzed by scanning electron microscopy.</p><p><strong>Results: </strong>The results showed that two of the three nanocomposites were effective in reducing biofilm formation (GOAg and GOZnO), although the nanofilms were characterized by the roughest surface, indicating that high surface roughness is unfavorable for biofilm formation by the tested bacterial species (<i>Staphylococcus aureus</i> (ATCC 25923), <i>Salmonella enterica</i> (ATCC 13076), <i>Pseudomonas aeruginosa</i> (ATCC 27853)).</p><p><strong>Conclusion: </strong>The performed analysis indicated that graphene oxide may be a platform for metal nanoparticles that enhances their properties (eg colloidal stability, which is maintained over time). Nanocomposites based on graphene oxide with silver nanoparticles and other types of nanocomposites with zinc oxide were effective against biofilms, contributing to changes throughout the biofilm structure, causing a significant reduction in the thickness of the structure, and affecting cell distribution. A nanocomposite consisting of graphene oxide with copper nanoparticles inhibited the biofilm, but to a lesser extent.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"17 ","pages":"303-320"},"PeriodicalIF":4.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Surfactant Structures and High-Speed Mixing Dynamics in Achieving Nano-Sized Emulsions with Simple High-Speed Homogenization.","authors":"Sakdinon Na Nan, Jittima Amie Luckanagul, Vipaporn Rakkanka Panapisal","doi":"10.2147/NSA.S492639","DOIUrl":"10.2147/NSA.S492639","url":null,"abstract":"<p><strong>Purpose: </strong>This study investigates the impact of various mixing parameters and surfactant combinations on the physical characteristics of nanoemulsions produced using high-speed homogenization. Nanoemulsions are explored for their capacity to enhance transdermal drug delivery in pharmaceutical and cosmetic contexts.</p><p><strong>Methods: </strong>Employing a standard high-speed homogenizer typical in the cosmetic industry, we tested different combinations of Polysorbate (Tween®) and Sorbitan ester (Span®) surfactants under single and intermittent process configurations. Key parameters assessed included particle size, size distribution, Oswald ripening, and creaming index.</p><p><strong>Results: </strong>Nanoemulsions synthesized had particle sizes below 200 nm and appeared as white liquids with slight creaming. Extended mixing times were associated with smaller droplet sizes and reduced creaming. Both processing methods yielded similar nanoemulsion properties, indicating minimal impact from the type of process used. The nanoemulsions exhibited polydispersity indices ranging from 0.3 to 0.5, suggesting a uniform size distribution, and showed negligible Oswald ripening, which indicates stable droplet sizes over time.</p><p><strong>Conclusion: </strong>The study confirms that precise control of mixing parameters and the selection of appropriate surfactant pairs are more critical than the choice of process in producing stable nanoemulsions. With efficient use of standard high-speed homogenizers, stable nanoemulsions suitable for large-scale production can be achieved, providing a cost-effective method for pharmaceutical and cosmetic industries.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"17 ","pages":"273-288"},"PeriodicalIF":4.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Highly Stretchable Ag-MWCNT Composite for Screen-Printed Textile Electronics with Improved Mechanical and Electrical Properties.","authors":"Daniel Janczak, Katarzyna Wójkowska, Tomasz Raczyński, Marcin Zych, Sandra Lepak-Kuc, Jerzy Szałapak, Mikko Nelo, Aleksandra Kądziela, Grzegorz Wróblewski, Heli Jantunen, Małgorzata Jakubowska","doi":"10.2147/NSA.S493579","DOIUrl":"10.2147/NSA.S493579","url":null,"abstract":"<p><strong>Introduction: </strong>The rapid growth of flexible and wearable electronics has created a need for materials that offer both mechanical durability and high conductivity. Textile electronics, which integrate electronic pathways into fabrics, are pivotal in this field but face challenges in maintaining stable electrical performance under mechanical strain. This study develops highly stretchable silver multi-walled carbon nanotube (Ag-MWCNT) composites, tailored for screen printing and heat-transfer methods, to address these challenges.</p><p><strong>Methods: </strong>Silver flakes dispersed in a thermoplastic polyurethane (TPU) matrix formed the base composite, which was initially evaluated under tensile and cyclic stretching conditions. Resistance drift observed in these tests prompted the incorporation of multi-walled carbon nanotubes (MWCNTs). Leveraging their high aspect ratio and conductivity, MWCNTs were homogenized into the composite at varying concentrations. The resulting Ag-MWCNT composites were assessed through cyclic stretching and thermal shock tests to evaluate electrical and mechanical performance.</p><p><strong>Results: </strong>Incorporating MWCNTs improved composite performance, reducing resistance change amplitude by 40% and stabilizing resistance within 2-8 Ohms under mechanical stress. These materials demonstrated superior electrical stability and durability, maintaining consistent performance over extended use compared to Ag/TPU alone.</p><p><strong>Discussion: </strong>This study highlights the critical role of MWCNTs in enhancing the reliability of conductive composites for textile electronics. By addressing resistance drift and stabilizing electrical properties, these advancements enable more robust and long-lasting wearable technologies. The demonstrated feasibility of combining screen-printing and heat-transfer techniques provides a scalable approach for manufacturing flexible electronics, paving the way for further innovation in industrial applications.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"17 ","pages":"289-302"},"PeriodicalIF":4.9,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan-Based Nanocapsules as a Delivery System of Hydrophobic Carnosic Acid, A Model Neuroprotective Drug.","authors":"Joanna Odrobińska-Baliś, Magdalena Procner, Kinga Krużel, Magdalena Regulska, Monika Leśkiewicz, Dorota Duraczyńska, Szczepan Zapotoczny, Władysław Lasoń, Krzysztof Szczepanowicz","doi":"10.2147/NSA.S490372","DOIUrl":"10.2147/NSA.S490372","url":null,"abstract":"<p><strong>Introduction: </strong>Since the population of Europe is rapidly aging, the number of cases of neurodegenerative diseases sharply increases. One of the most significant limitations of current neurodegenerative disease treatment is the inefficient delivery of neuroprotective drugs to the affected part of the brain. One of the promising methods to improve the pharmacokinetic and pharmacodynamic properties of antioxidants is their encapsulation in nanocarriers.</p><p><strong>Materials and methods: </strong>Encapsulation of carnosic acid into a chitosan-based nanoparticle system with ultrasound-assisted emulsification process was developed. The physicochemical properties (size, stability, concentration of nanoparticles) of obtained nanocapsules were analyzed. Also, the cytotoxicity and neuroprotective effect in SH-SY5Y cells exposed to toxic concentration of H₂O₂ of the obtained nanoparticles were evaluated in vitro.</p><p><strong>Results and discussion: </strong>The capsules with diameters between 90 and 150 nm and long-term stability were obtained. Cytotoxicity tests of empty capsules indicate that observed toxic effects were concentration dependent and lower concentrations (dilution above 500×) can be considered as safe for tested cells. Our study also indicates that encapsulation of carnosic acid decreased the cytotoxicity of empty nanocapsules and can efficiently protect SH-SY5Y cells from factors causing cell destruction. In addition, the neuroprotective efficacy of carnosic acid loaded nanocapsules was also demonstrated in SH-SY5Y cells exposed to toxic concentration of H₂O₂. The designed nanoparticles appear to possess sufficient biocompatibility to deserve their further evaluation in in vivo models.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"17 ","pages":"259-271"},"PeriodicalIF":4.9,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Binding Buffer Composition (Polyethylene Glycol, Sodium Chloride and pH) for Extraction of DNA from Biological Fluids Using Polyethyleneimine Functionalized Iron Oxide Nanoparticle-Based Method.","authors":"Imran Khan, Gaurav Kaushik, Chaitenya Verma, Richa Vashishtha, Vinay Kumar","doi":"10.2147/NSA.S494613","DOIUrl":"10.2147/NSA.S494613","url":null,"abstract":"<p><strong>Introduction: </strong>Efficient extraction of DNA from biological fluids is crucial for applications in molecular biology, forensic science, and clinical diagnostics. However, traditional DNA extraction methods often require costly reagents and lengthy procedures. This study aims to optimize the binding buffer composition for DNA extraction using polyethyleneimine-coated iron oxide nanoparticles (PEI-IONPs), which offer the dual benefits of magnetic separation and high DNA-binding efficiency.</p><p><strong>Methods: </strong>The effects of three key binding buffer components-polyethylene glycol (PEG-6000), sodium chloride (NaCl), and pH-on DNA adsorption efficiency were systematically evaluated. Blood samples were treated with PEI-IONPs under various conditions, and DNA concentration, yield, and purity were quantified. Nanoparticle functionalization was confirmed through characterization, and DNA quality was validated via agarose gel electrophoresis.</p><p><strong>Results: </strong>The optimized binding buffer composition consisted of a PEG-6000 concentration of 30%, NaCl concentration of 0M, and pH of 4, which yielded the highest DNA concentration (34 ± 1.2 ng/μL), yield (6.8 ± 0.2 μg), and purity (A260/A280 ratio of 1.81). These conditions significantly improved DNA recovery compared to suboptimal buffer compositions.</p><p><strong>Conclusion: </strong>The findings highlighted the critical role of binding buffer composition in maximizing DNA recovery. The use of optimized PEI-IONPs provided a rapid and efficient method for DNA extraction, supporting its potential for applications in scientific and clinical research. Future studies should explore the robustness of these optimized conditions across diverse biological fluids and extraction settings.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"17 ","pages":"247-258"},"PeriodicalIF":4.9,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}