Nanotechnology, Science and Applications最新文献

{"title":"Tailoring CdO-CuO-ZnO Mixed Metal Oxide Nanocomposites for Anticancer Activity via Co-Precipitation Method.","authors":"Shadha Nasser Aziz, Abduh Mohammad Abdulwahab, Thana Shuga Aldeen, Abdullah Ahmed Ali Ahmed","doi":"10.2147/NSA.S519229","DOIUrl":"https://doi.org/10.2147/NSA.S519229","url":null,"abstract":"<p><strong>Introduction: </strong>The use of metal oxide nanoparticles as anticancer agents is of great interest due to their unique properties that allow targeted delivery at low concentrations with minimal toxicity to healthy cells.</p><p><strong>Methods: </strong>In this work, CdO-CuO-ZnO mixed metal oxide nanocomposites were synthesized by the co-precipitation method, and their structural and optical properties, along with their anticancer activity, were investigated. The samples were characterized by X-ray diffraction (XRD), total reflection X-ray fluorescence (TXRF), transmission electron microscopy (TEM), selected area electron diffraction (SAED), UV-Vis spectroscopy, electrometer/high resistance material, and vibrating sample magnetometers (VSM).</p><p><strong>Results: </strong>X-ray diffraction (XRD) measurements showed that CdO exhibits a cubic structure, CuO possesses a monoclinic structure, ZnO displays a hexagonal structure, and the mixture showed peaks corresponding to all three oxides. TEM images revealed that the prepared nanoparticles have quasi-spherical shapes. Anticancer studies confirmed that the CdO-CuO-ZnO nanocomposite demonstrates excellent cytotoxicity, with moderate activity against human colon (Caco-2) and lung (A549) cancer cell lines, exhibiting IC50 values of 10.57 μg/mL and 6.61 μg/mL, respectively.</p><p><strong>Conclusion: </strong>Our study shows that the prepared CdO-CuO-ZnO nanocomposite has massive potential in cancer therapy.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"225-244"},"PeriodicalIF":4.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-Synthesized Nanomaterials for Aflatoxin Mitigation: A Review.","authors":"Yohannes Gelaye, Huaiyong Luo","doi":"10.2147/NSA.S520121","DOIUrl":"https://doi.org/10.2147/NSA.S520121","url":null,"abstract":"<p><p>Aflatoxin contamination poses a significant challenge to global food safety, public health, and agricultural sustainability. Traditional methods for mitigating aflatoxins, such as chemical and physical detoxification techniques, often raise concerns about environmental harm, nutrient loss, and potential toxicity. In contrast, green-synthesized nanomaterials have emerged as an environmentally friendly and effective solution for controlling aflatoxins. This study explores the potential of green-synthesized nanomaterials for aflatoxin mitigation, focusing on their mechanisms of action, effectiveness, and long-term applicability in agricultural and food safety contexts. A comprehensive review of 116 articles on the latest developments in green nanotechnology was used, focusing on the creation, characterization, and application of nanoparticles, including silver, zinc oxide, titanium dioxide, and iron-based nanomaterials. Green nanoparticles reduce aflatoxin load primarily through their antioxidant properties, which neutralize oxidative stress, and their high adsorption capacity, which binds aflatoxins and reduces their bioavailability. Photocatalytic degradation, adsorption, and enzymatic detoxification were also evaluated. The results indicate that green-synthesized nanoparticles exhibit high efficacy, biocompatibility, and minimal environmental impact, especially when compared to traditional detoxification methods. However, challenges such as nanoparticle stability, large-scale production, regulatory issues, and potential long-term toxicity still require further investigation. To advance this field, future studies should focus on refining green synthesis processes, enhancing nanoparticle stability, and exploring the integration of nanotechnology with biosensors and smart packaging for real-time aflatoxin monitoring. By advancing these sustainable technologies, this research aims to contribute to the development of effective and safe methods for aflatoxin mitigation, thereby supporting global food security, public health, and environmental sustainability.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"211-223"},"PeriodicalIF":4.9,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Recent Progress on Nickel@Silver Metals Core@Shell Nanoparticles Application in Printed Conductive Materials - A Mini-Review.","authors":"Anna Pajor-Świerzy, Krzysztof Szczepanowicz","doi":"10.2147/NSA.S509925","DOIUrl":"https://doi.org/10.2147/NSA.S509925","url":null,"abstract":"<p><p>This mini-review paper gives a brief summary of recent works in the development of bimetallic core@shell nanoparticles composed of nickel (as a core) and a silver shell (Ni@Ag NPs). We present the methods of Ni@Ag NPs synthesis, ink preparation, and their coatings formation. We also place emphasis on the selection and optimization of the sintering process of materials based on Ni@Ag NPs. Finally, the challenges in the application of Ni@Ag NPs in printed conductive structures are presented.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"197-210"},"PeriodicalIF":4.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12007610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Computational Modeling of Fe₃O₄@Au Nanoparticles in Hyperthermia Treatment of Skin Cancer.","authors":"Piotr Gas, Muhammad Suleman, Farah Khaliq","doi":"10.2147/NSA.S495377","DOIUrl":"https://doi.org/10.2147/NSA.S495377","url":null,"abstract":"<p><strong>Background: </strong>Nanotechnology can be used to treat a diversity of cancers with different physiological properties. Skin cancers are common among people affected by an excessive solar radiation of the ultraviolet (UV) range.</p><p><strong>Introduction: </strong>This paper describes a mathematical formulation and simulation approach for the magnetic hyperthermia therapy of skin cancer using gold-coated iron oxide (Fe₃O₄@Au) magnetic nanoparticles (MNPs).</p><p><strong>Methods: </strong>The authors created an artificial 3D geometry model of skin cancer with tissue-mimicking materials, constructed a mesh, and solved all the required physics for electro-thermal simulation using FEM-based software. The heat transfer in the skin tissue was modeled using the Pennes bioheat equation, and the Helmholtz-type equation of quasi-static magnetic field produced by a three-turned coil surrounding the tumor.</p><p><strong>Results: </strong>The simulated magnetic field pattern was compared with that of the analytical solution along the symmetry axis of the helical coil with good agreement. The obtained results show that the tumor damage is maximum in the tumor center and decreases towards its outer boundaries. Additionally, the impact of varying values of blood perfusion rate, blood density, blood specific heat capacity, heat dissipation produced by Fe₃O₄@Au MNPs, and metabolic heat generation has been examined for thermal therapy. The performed simulations show that all these parameters influences heating characteristics of tumor tissues by gold-coated magnetic nanoparticles.</p><p><strong>Conclusion: </strong>Gold-iron oxide magnetic nanoparticles succeeded to damage 90-99% skin cancer. Among all the contributing parameters, the blood perfusion is the most sensitive parameter in thermal therapy of skin tumor.</p><p><strong>Recommendations: </strong>On the bases of results obtained, we recommend physicians to use Fe₃O₄@Au MNPs in real time medical skin cancer treatments.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"173-196"},"PeriodicalIF":4.9,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Anti-Aging Potential of <i>Dendrobium</i> Species and Novel Microemulsion Delivery of <i>Dendrobium kentrophyllum</i> Extract for Anti-Aging Effect.","authors":"Suradwadee Thungmungmee, Boonchoo Sritularak, Nakuntwalai Wisidsri, Nattakan Kanana, Nattika Nimmano","doi":"10.2147/NSA.S508582","DOIUrl":"10.2147/NSA.S508582","url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to investigate in vitro anti-aging activities of 29 <i>Dendrobium</i> spp. and develop and characterize microemulsions (MEs) for topical application.</p><p><strong>Methods: </strong>Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), H₂O₂ scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated. Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2',7'-dichlorofluorescin diacetate (DCFDA) assays, respectively. <i>D. kentrophyllum</i> extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.</p><p><strong>Results: </strong><i>D. kentrophyllum</i> appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and anti-elastase activities. The extract was safe for human skin cells at a concentration of 6.25-100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.</p><p><strong>Conclusion: </strong>DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"157-171"},"PeriodicalIF":4.9,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11951923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Germination and Growth of Chrysanthemum Synthetic Seeds Through Iron Oxide Nanoparticles and Indole-3-Acetic Acid: Impact of Treatment Duration on Metabolic Activity and Genetic Stability.","authors":"Dariusz Kulus, Alicja Tymoszuk, Katarzyna Gościnna, Magdalena Osial","doi":"10.2147/NSA.S503868","DOIUrl":"10.2147/NSA.S503868","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the effects of pure iron oxide nanoparticles (Fe₃O₄ NPs), citrate-stabilized iron oxide nanoparticles (Fe₃O₄CA NPs), and indole-3-acetic acid (IAA), applied at various time regimes, on the germination, growth, and ex vitro development of chrysanthemum synthetic seeds. The genetic and metabolic stability of the plants was also assessed.</p><p><strong>Methods: </strong>Nodal segments of <i>Chrysanthemum × morifolium</i> /Ramat./ Hemsl. 'Richmond', with a single axillary bud, were encapsulated in 3% calcium alginate with the addition of IAA (1 mg·L^-1) and/or NPs (7.7 mg·L^-1). The synthetic seeds were cultured in vitro for 30 or 60 days on a water-agar medium and then transplanted to the greenhouse for further analyses.</p><p><strong>Results: </strong>Results indicated that IAA and Fe₃O₄CA NPs applied singularly significantly enhanced germination rates (83.33-92.18%) compared with the IAA- and NP-free control (56.67-64.18%), regardless of treatment time. The simultaneous use of IAA and Fe₃O₄CA NPs promoted longer shoot development after 30 days of treatment but showed negative effects after extended exposure. The same combination improved rooting efficiency compared to IAA alone. Supplementation with NPs improved acclimatization rates for younger plants but had variable effects on older plants. Leaf growth metrics were enhanced with Fe₃O₄CA NPs in plants after 30 days of treatment, yet no significant differences were observed in leaf dimensions after 60 days. The content of flavonoids, anthocyanins, and chlorophyll was affected by the exposure duration. Biochemical analyses revealed increased total polyphenol content and antioxidant capacity (FRAP, ABTS) in treated plants, particularly with IAA and Fe₃O₄CA NPs. Start codon targeted (SCoT) analyses showed no polymorphisms among treated plants, confirming their genetic stability.</p><p><strong>Conclusion: </strong>The study found that the combination of IAA and Fe₃O₄CA NPs improved germination and shoot development in chrysanthemum synthetic seeds, while maintaining genetic stability, although prolonged exposure negatively affected plant growth metrics.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"139-155"},"PeriodicalIF":4.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Approach to Enhance the Solubility of an Atypical Antipsychotic Drug, Aripiprazole: Design, Characterization, and Evaluation of Arabinoxylan-Based Nanoparticles.","authors":"Mehwish Sikander, Ume Ruqia Tulain, Nadia Shamshad Malik, Arshad Mahmood, Mohammed S Alqahtani, Alia Erum, Muhammad Tariq Khan","doi":"10.2147/NSA.S502002","DOIUrl":"10.2147/NSA.S502002","url":null,"abstract":"<p><strong>Introduction: </strong>Natural polymers have emerged as versatile and sustainable alternatives to synthetic polymers in pharmaceutical and biomedical applications. This study focuses on the extraction of arabinoxylan (AX) from maize husk and its potential as a promising excipient to enhance the solubility and oral bioavailability of Aripiprazole (APZ), a poorly water-soluble antipsychotic drug, offering a robust strategy for overcoming challenges associated with hydrophobic drugs.</p><p><strong>Methods: </strong>APZ-loaded AX nanoparticles were synthesized using the ionotropic gelation technique. The formulation with the highest encapsulation efficiency designated as FN4 was selected for detailed characterization. Various analytical techniques, including Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), and Differential Scanning Calorimetry (DSC), were employed to assess the morphological, crystalline, and thermal properties of the nanoparticles. In vitro release studies were conducted on both simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 6.8) to evaluate drug dissolution behaviour. The everted sac method was utilized to assess the permeation and transport of APZ from the AX-based nanoparticles.</p><p><strong>Results: </strong>The FN4 formulation exhibited an encapsulation efficiency of 88.9% ± 1.77%, with a particle size of 284.4 nm, a polydispersity index (PDI) of 0.346, and a zeta potential of 20.7 mV. SEM analysis revealed a uniform distribution of polyhedral-shaped nanoparticles. XRD and DSC analyses indicated that APZ was in an amorphous state within the nanoparticles. Drug release was more pronounced at pH 6.8, with the AX nanoparticles showing sustained release. The everted sac method demonstrated enhanced permeation of APZ across intestinal membranes, supporting the potential of AX nanoparticles in improving drug absorption.</p><p><strong>Discussion: </strong>The AX-based nanoparticle formulation significantly improved the solubility, pH-dependent release profile, and sustained release of APZ, offering a promising strategy to enhance the oral bioavailability of poorly soluble drugs. These findings suggest that AX nanoparticles could serve as an effective delivery system for enhancing the therapeutic potential of hydrophobic drugs like APZ.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"115-137"},"PeriodicalIF":4.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Metal Nanoparticles Using <i>Cinnamomum</i>-Based Extracts and Their Applications.","authors":"Omar Samir Mohamed Megahed Saleh Elmitwalli, Deyari Azad Kareem Kassim, Ahmed Taymour Algahiny, Fryad Zeki Henari","doi":"10.2147/NSA.S489274","DOIUrl":"https://doi.org/10.2147/NSA.S489274","url":null,"abstract":"<p><strong>Introduction: </strong>Nanotechnology is the science that deals with matter on the nanoscale, with sizes ranging from 1 to 100 nm. It involves designing, synthesising, characterising and applying these nanoscale materials. Nanoparticles (NPs) are known for their high surface-area to volume-ratio, surface charge density, low melting point, and distinguishably good optical/electrical properties. NPs exhibit an excellent drug delivery system, an effective contrast agent for vascular imaging, and effective antimicrobial activity. The biological synthesis of NPs is a simple, cost-effective, and environmentally friendly technique. This bottom-up technique utilises organisms' enzymes/bio-compounds and a plant extract as capping and reducing agents. <i>Cinnamomum</i> species are known for their intrinsic antimicrobial, antidiabetic, antioxidant, anti-inflammatory, anticancer, and neuroprotective properties. This review summarises articles that greenly synthesised NPs using <i>Cinnamomum</i> species' extracts, describing their methodologies, characterisation of the nanoparticles and their medical applications.</p><p><strong>Methods: </strong>A literature search has been conducted on databases PubMed, ScienceDirect, and Frontier on the green synthesis of metal nanoparticles (MNPs) using <i>Cinnamomum</i>-based extracts. Various articles reported the methodology of utilising <i>Cinnamomum</i> species' extracts as reducing and capping agents. Only original lab articles were considered.</p><p><strong>Results: </strong>Various types of MNPs have been successfully synthesised. The most common <i>Cinnamomum</i> species utilised as extracts is <i>Cinnamomum tamala</i>. The most common applications tested were the MNPs' antibacterial, antiviral, antifungal, antidiabetic and anticancerous activity. MNPs also had a role in treating mice-induced polycystic ovarian syndrome and Parkinson-like neurodegenerative diseases.</p><p><strong>Conclusion: </strong><i>Cinnamomum</i> species have been successfully utilised in the green synthesis of various MNPs. Silver and Gold NPs were the most reported. These MNPs proved their efficacy in multiple fields of medicine and biology, especially their antibacterial, antiviral and antifungal activity. Notably, the newly synthesised NPs showed promising results in treating polycystic ovarian syndrome in rats.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"93-114"},"PeriodicalIF":4.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Stability and Reusability of Subtilisin Carlsberg Through Immobilization on Magnetic Nanoparticles.","authors":"Hassan Khan, Ihtisham Ul Haq, Zahid Khan, Muhammad Nughman, Zia Ur Rehman, Taj Ali Khan, Saadullah Khan, Mamdouh Allahyani, Naif Alsiwiehri, Mohammed A Alshamrani, Aamir Shehzad, Noor Muhammad","doi":"10.2147/NSA.S499101","DOIUrl":"10.2147/NSA.S499101","url":null,"abstract":"<p><strong>Introduction: </strong>Immobilizing enzymes on solid supports such as magnetic nanoparticles offers multi-dimensional advantages, including enhanced conformational, structural, and thermal stability for long-term storage and reusability.</p><p><strong>Methodology: </strong>The gene encoding subtilisin Carlsberg was isolated from proteolytic <i>Bacillus haynesii</i>, a bacterium derived from salt mines. The nucleotide sequence encoding pro-peptide and mature protein were cloned into pET22(a)+ vector and expressed in <i>E. coli</i>. The extracted enzyme was subsequently immobilized on glutaraldehyde-linked-chitosan-coated magnetic nanoparticles.</p><p><strong>Results: </strong>Fourier-transform infrared analysis revealed higher intensity peaks for the enzyme-immobilized nanoparticles indicating an increase in bonding numbers. X-ray diffraction analysis revealed a mild amorphous state for immobilized nanoparticles in contrast to a more crystalline state for free nanoparticles. An increased mass content and atomic percentage for carbon and nitrogen were recorded in EDX analysis for enzyme immobilized magnetic nanoparticles. Dynamic light scattering analysis showed an increase in average particle size from ~85 nm to ~250 nm. Upon enzyme immobilization, the Michaelis-Menten value increased from 11.5 mm to 15.02 mM, while the maximum velocity increased from 13 mm/min to 22.7 mm/min. Immobilization significantly improved the thermostability with 75% activity retained by immobilized enzyme at 70 °C compared to 50% activity by free enzyme at the same temperature. Immobilization yield, efficiency and activity recovery were 61%, 84% and 51%, respectively. The immobilized enzyme retained 70% of its activity after 10 cycles of reuse, and it maintained 55% of its activity compared to 50% activity by free enzyme after 30 days of storage.</p><p><strong>Conclusion: </strong>The present study highlights the efficacy of magnetic nanoparticle-based immobilization in enhancing enzyme functioning and facilitates its incorporation into commercial applications necessitating high stability and reusability, including detergents, medicines, and bioremediation.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"71-91"},"PeriodicalIF":4.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Nanofibers for the Delivery of Endolysin/Dendronized Ag-NPs Complex Against Pseudomonas aeruginosa.","authors":"Magdalena Lasak, Małgorzata Łysek-Gładysińska, Karolina Lach, Viraj P Nirwan, Dorota Kuc-Ciepluch, Javier Sanchez-Nieves, Francisco Javier de la Mata, Amir Fahmi, Karol Ciepluch","doi":"10.2147/NSA.S498942","DOIUrl":"10.2147/NSA.S498942","url":null,"abstract":"<p><strong>Purpose: </strong>As bacterial resistance to antibiotics increases, there is an urgent need to identify alternative antibacterial agents and improve antibacterial materials. One is the controlled transport of antibacterial agents that prevents infection with drug-resistant bacteria, especially in the treatment of difficult-to-heal wounds.</p><p><strong>Methods: </strong>This work presents the use of electrospun PLCL/PVP (poly(L-lactide-co-ε-caprolactone/polyvinylpyrrolidone) nanofibers modified with two agents with antibacterial properties but with different mechanisms of action, that is, dendritic silver nanoparticles (Dend-AgNPs) and endolysin.</p><p><strong>Results: </strong>The nanomat prepared in this manner showed significant antibacterial activity against antibiotic-resistant Pseudomonas aeruginosa strains, inhibiting their growth and production of key pigments and virulence factors. Moreover, the use of nanofibers as carriers of the selected factors significantly reduced their cytotoxicity towards human fibroblasts.</p><p><strong>Conclusion: </strong>The results confirmed the possibility of using the presented product as an innovative dressing material, opening new perspectives for the treatment of wounds and combating bacterial infections with drug-resistant bacteria.</p>","PeriodicalId":18881,"journal":{"name":"Nanotechnology, Science and Applications","volume":"18 ","pages":"57-70"},"PeriodicalIF":4.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}