Electrospun Nanofibers for the Delivery of Endolysin/Dendronized Ag-NPs Complex Against Pseudomonas aeruginosa.

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY

Nanotechnology, Science and Applications Pub Date : 2025-02-18 eCollection Date: 2025-01-01 DOI:10.2147/NSA.S498942

Magdalena Lasak, Małgorzata Łysek-Gładysińska, Karolina Lach, Viraj P Nirwan, Dorota Kuc-Ciepluch, Javier Sanchez-Nieves, Francisco Javier de la Mata, Amir Fahmi, Karol Ciepluch

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引用次数: 0

Abstract

Purpose: As bacterial resistance to antibiotics increases, there is an urgent need to identify alternative antibacterial agents and improve antibacterial materials. One is the controlled transport of antibacterial agents that prevents infection with drug-resistant bacteria, especially in the treatment of difficult-to-heal wounds.

Methods: This work presents the use of electrospun PLCL/PVP (poly(L-lactide-co-ε-caprolactone/polyvinylpyrrolidone) nanofibers modified with two agents with antibacterial properties but with different mechanisms of action, that is, dendritic silver nanoparticles (Dend-AgNPs) and endolysin.

Results: The nanomat prepared in this manner showed significant antibacterial activity against antibiotic-resistant Pseudomonas aeruginosa strains, inhibiting their growth and production of key pigments and virulence factors. Moreover, the use of nanofibers as carriers of the selected factors significantly reduced their cytotoxicity towards human fibroblasts.

Conclusion: The results confirmed the possibility of using the presented product as an innovative dressing material, opening new perspectives for the treatment of wounds and combating bacterial infections with drug-resistant bacteria.

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电纺丝纳米纤维递送抗铜绿假单胞菌的内溶素/树突化Ag-NPs复合物。

目的：随着细菌对抗生素耐药性的增加，迫切需要寻找替代抗菌剂和改进抗菌材料。一是控制抗菌剂的运输，防止耐药细菌的感染，特别是在治疗难以愈合的伤口时。方法：采用树突银纳米粒子（dendagnps）和内溶素两种具有抗菌性能但作用机制不同的改性剂，对电纺丝PLCL/PVP（聚l -乳酸-co-ε-己内酯/聚乙烯吡罗烷酮）纳米纤维进行改性。结果：该方法制备的纳米菌对耐药铜绿假单胞菌具有明显的抑菌活性，抑制其生长、关键色素和毒力因子的产生。此外，使用纳米纤维作为选定因子的载体显著降低了它们对人成纤维细胞的细胞毒性。结论：证实了该产品作为一种创新敷料的可能性，为伤口治疗和抗耐药菌感染开辟了新的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-

CiteScore

11.70

自引率

0.00%

发文量

审稿时长

16 weeks

期刊介绍： Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.