Molecular Biotechnology最新文献

{"title":"Oncogenic Gene CNOT7 Promotes Progression and Induces Poor Prognosis of Glioma.","authors":"Feng Lu, Xiulong Jiang, Kun Lin, Pengfeng Zheng, Shizhong Wu, Guangming Zeng, De Wei","doi":"10.1007/s12033-024-01223-5","DOIUrl":"10.1007/s12033-024-01223-5","url":null,"abstract":"<p><p>Glioma is the most common malignant brain tumor in the central nervous system with the poor prognosis of patients. The CNOT7 (CCR4-NOT Transcription Complex Subunit 7) is an important functional subunit of CCR4-NOT protein complex that has not been reported in glioma. In this study, we aimed to explore the function of CNOT7 in glioma. The TCGA (The Cancer Genome Atlas) and CGGA (Chinese Glioma Genome Atlas) databases were used for investigating the expression and survival condition of CNOT7 in glioma. The cellular function experiments of qRT-PCR, CCK-8 assays, wound healing assays, and Transwell assays were conducted to verify the function of knockdown CNOT7 in the glioma cell lines DBTRG and U251. Enrichment analysis was used to explore the molecular mechanism of CONT7 in glioma. What is more, the upstream regulation transcription factors of CNOT7 were analyzed based on the ChIP-Atlas and cBioportal (provisional) databases, and verified by the qRT-PCR and luciferase reporter assay. The CNOT7 was highly expressed in glioma and presented the poorer prognosis. The knockdown of CNOT7 inhibited the proliferation, migration, and invasion of glioma cell line, compared to control group. The enrichment analysis revealed that the CNOT7 participated in the development of glioma via G2M checkpoint, E2F targets, IL6-JAK-STAT3, and TNF-α signaling pathways via NF-κB. Besides, it was found that the HDAC2 (Human histone deacetylase-2) contributes to increased CNOT7 expression in glioma. The high-expressed CNOT7 is an oncogene with poor prognosis and participate the progression of glioma.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2607-2616"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression, Sarkosyl Solubilization, DNase Activity, Purification, and SPR Binding Affinity of Recombinant Diphtheria Toxoid (rCRM197EK) Expressed in Escherichia coli BL21(DE3).","authors":"Mia Tria Novianti, Toto Subroto, Yusuf Sofyan Efendi, Umi Baroroh, Shinta Kusumawardani, Gilang Gumilar, Muhammad Yusuf, Shabarni Gaffar","doi":"10.1007/s12033-024-01238-y","DOIUrl":"10.1007/s12033-024-01238-y","url":null,"abstract":"<p><p>CRM197EK is a derivative of diphtheria toxoid cross-reactive material-197 (CRM197) with two-point mutations (K51E and E148K) to improve its properties for a vaccine conjugate and drug delivery. A previous study has shown that intracellularly expressing CRM197EK in Escherichia coli (E. coli) host formed inclusion bodies that need a complicated purification and refolding step. Protein purification from inclusion bodies can be overcome by solubilization of inclusion bodies by using N-lauroyl sarcosine (sarkosyl). In this work, recombinant CRM197EK (rCRM197EK) was expressed in E. coli BL21 (DE3) as inclusion bodies, then solubilized using sarkosyl to form a soluble rCRM197EK without the need for a renaturation process. Furthermore, rCRM197EK was purified using the Ni-NTA column, characterized by SDS-PAGE and Western Blot, and its biological activity was assayed through its DNase activity. Moreover, its binding affinity with anti-diphtheria toxin (DT) antibody was measured using the surface plasmon resonance (SPR). The result showed that solubilization with sarkosyl form soluble rCRM197EK (61.61 kDa) was confirmed by SDS-PAGE and Western Blot with a yield of 2.8 mg/mL. rCRM197EK shows DNase activity, and the SPR assay shows that it can interact with an anti-DT antibody with a binding energy of - 9.2 kcal/mol.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2774-2784"},"PeriodicalIF":2.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming Hemophilia Management: Lessons from Gene Therapy Clinical Trials.","authors":"Md Sadique Hussain, Mudasir Maqbool, Mohammed M Arab, Amita Joshi Rana, Sumel Ashique, Yumna Khan, Vikas Jakhmola, Gaurav Gupta","doi":"10.1007/s12033-025-01464-y","DOIUrl":"https://doi.org/10.1007/s12033-025-01464-y","url":null,"abstract":"<p><p>Gene therapy signifies a transformative revolution in hemophilia care, providing the possibility for sustained endogenous synthesis of coagulation factors and limiting the need for external factor supplementation. Preliminary experiments in hemophilia B via adeno-associated viral (AAV) vectors encountered constraints owing to immunological reactions and temporary translation. Progress in vector technology, particularly via self-complementary AAV innovation and codon-optimized mini-factor IX (FIX) concepts, has markedly improved transduction performance and prolonged FIX activity. Initial investigations have shown encouraging outcomes, with certain individuals sustaining consistent FIX expressions for more than 8 years; hence, decreasing yearly bleeding incidents and requiring preventive therapy. The development of gene therapy for hemophilia A has encountered substantial obstacles owing to the enormous size of the factor VIII (FVIII) gene. The recent experiments using AAV serotypes 5 (AAV5) vectors that encode B-domain-deleted FVIII constructs have shown sustained levels along with substantial decreases in hemorrhage incidents. Research has shown prolonged FVIII expression, with some individuals attaining almost normal coagulation efficiency. Phase III studies have validated long-term effectiveness and safety, with transient transaminase elevations being the most common adverse event. Notwithstanding these advancements, difficulties persist, including immunological reactions to vector capsids, hepatotoxicity, and unpredictability in translation levels. Innovative approaches including lentiviral vectors, gene-editing technologies, and novel customized connection strategies demonstrate possibilities for enhancing the effectiveness of gene therapy. Continuous clinical research and improvement in delivery systems will be crucial in substantiating gene therapy as a definitive approach for hemophilia.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Small and Resilient Nucleic Acid-Binding Protein from Nanoarchaeum equitans.","authors":"Klaudia Marlicka, Katarzyna M Serafin, Julia A Sobieska, Julia M Szeptycka, Julia E Szumska, Kornelia Dobrzyńska-Kobylec, Marcin Olszewski, Katarzyna P Szymańska","doi":"10.1007/s12033-025-01458-w","DOIUrl":"https://doi.org/10.1007/s12033-025-01458-w","url":null,"abstract":"<p><p>Nucleic acid-binding proteins (NBPs) are essential biomolecules in all organisms. They play an important role in gene regulation and expression in cells. One type of these proteins is DNA- and RNA-binding proteins, known as DRBPs. In recent years, research has started to focus more on this particular group and new DRBPs have been identified successively. Their special dual-binding abilities are used in biotechnology and medicine. They can be applied for gene editing and drug development in treating such diseases as cancer. One of the NBP is Neq150, which comes from the Archaea strain Nanoarchaeum equitans. In our study, we purified the recombinant Neq150 with IMAC chromatography and assessed its binding ability to various forms of nucleic acids with EMSA. Thus, we concluded that Neq150 can be classified as a DRBP. Moreover, it exhibits thermostability at high temperatures (3 h at 99 °C) and significant long-term stability under various conditions. These results indicate that the Neq150 protein is a promising candidate for many applications in biotechnology and molecular biology, for example, as an auxiliary protein. The fusion of the DNA polymerase with another NBP protein from N. equitans (NeqSSB) has been previously well characterized, showing potential for similar possible applications for the Neq150.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming Multidrug Resistance in E. coli and Salmonella Isolates from Nile Tilapia: Synergistic Effects of Novel Antibiotic Combinations.","authors":"Laiba Shafique, Muhammad Farhan Khan, Shakeela Parveen, Youhou Xu, Peng Zhu","doi":"10.1007/s12033-025-01462-0","DOIUrl":"https://doi.org/10.1007/s12033-025-01462-0","url":null,"abstract":"<p><p>Escherichia coli and Salmonella are significant foodborne zoonotic pathogens, causing serious human illness. The rising global prevalence of antimicrobial resistance (AMR) in these species exacerbates their public health risk, complicating the treatment of bacterial infection. This study investigates its prevalence, resistant genes, and treatment strategy against antibiotic-resistant bacteria, focusing on E. coli and Salmonella isolates from Nile tilapia. Prevalence of E. coli and Salmonella was found to be 32 and 22% respectively. Antibiotic susceptibility testing revealed resistance to five antibiotics in E. coli and four in Salmonella. Physiochemical properties of antibiotic resistance genes (ABRGs) indicated that the TetB gene has the highest aliphatic index in both bacteria, suggesting greater stability. All Bla proteins were hydrophobic as indicated by negative GRAVY values, which may contribute to antibiotic efflux or modification of antibiotic targets. Motif analysis identified functional domains, and cellular localization prediction showed that TetA and TetB genes are primarily expressed in the cell membrane. To combat this resistance, a checkerboard method was used to explore novel antibiotic combinations. For E. coli, one synergistic and two additive combinations were identified, while for Salmonella, two synergistic and one additive combination were effective. These results highlight the importance of regularly evaluating antibiotic combinations to combat resistance and preserve antibiotic efficacy.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the Unique Epitopes: Validation of In-Silico Synthesized Tetravalent Dengue Vaccine (dvac) Through Cloning, Expression, and Immunogenic Analysis.","authors":"Sitara Nasar, Saima Iftikhar, Muhammad Shahid Nadeem","doi":"10.1007/s12033-025-01469-7","DOIUrl":"https://doi.org/10.1007/s12033-025-01469-7","url":null,"abstract":"<p><p>Dengue is an arthropod borne viral illness which infects millions of people each year. Despite the huge global losses, an effective medication is still unavailable against the disease. For vaccine development, reverse vaccinology approach is being tremendously used as it saves time, energy, cost and resources by selecting the potential epitopes for developing an effective vaccine formulation. In our previous study, we have also used reverse vaccinology approach to propose a multi-epitope vaccine formulation (dvac) against dengue, by targeting the conserved epitopes from all of its serotypes. In this study, we have performed population coverage analysis and molecular dynamic simulation studies which predicted that dvac could offer protection to 98.5% of world population against dengue and forms stable interactions with TLR3/TLR4 receptors, respectively. Moreover, we have performed in-lab gene cloning and expression analysis of dvac in Escherichia coli. The purified dvac is subjected to anti-NS1, anti-dNS1 and anti-EDIII antibodies, raised in rabbits, and the ELISA results confirmed the individuality and intactness of the epitopes incorporated in the refolded dvac molecule. The results from this study endorse the use of dvac as a potential dengue vaccine candidate and also support the reverse vaccinology approach for developing novel therapeutic formulations.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaping the Plant Specialized Metabolites Through Modern Breeding Technique.","authors":"Sayanti Mandal","doi":"10.1007/s12033-025-01455-z","DOIUrl":"https://doi.org/10.1007/s12033-025-01455-z","url":null,"abstract":"<p><p>Plant specialized metabolites (PSMs) have significantly diversified throughout evolutionary history and are regarded as crucial contributors to intricate interactions between plants and the changing environment. The chemical nature of these metabolites has been extensively investigated and used in agriculture, crop improvement, food industry, and pharmaceutical research, among other fields. These PSMs, often synthesized in response to abiotic stressors, function as protective agents against abiotic stresses under climate change. Therefore, this review aimed to elucidate the stress response in plants that leads to the synthesis of PSMs, including glucosinolates, carotenoids, phenolic, alkaloids, and flavonoids, which improve antioxidant efficiency by alleviating oxidative stress, a significant secondary stressor associated with major abiotic challenges such as salinity, drought, cold, and high temperatures. Since natural plants do not produce these metabolites in large quantities, many biotechnology-based strategies were investigated to increase their production. Additionally, we explore the genome editing advancements in engineering secondary metabolite pathways that have created novel possibilities for sustainable metabolite production. Moreover, plant biologist can enhance PSMs biosynthesis and identify novel metabolites by leveraging stress responses using CRISPR technology. These technological advances offer potential solutions for addressing global challenges in agriculture, medicine, and environmental sustainability.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144285524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailoring Corynebacterium glutamicum for Sustainable Biomanufacturing: From Traditional to Cutting-Edge Technologies.","authors":"Eldin Kurpejović, Berna Sariyar Akbulut, Fatma Gizem Avci","doi":"10.1007/s12033-025-01447-z","DOIUrl":"https://doi.org/10.1007/s12033-025-01447-z","url":null,"abstract":"<p><p>As the workhorse of industrial amino acid production, Corynebacterium glutamicum is the focus of this review, which provides a comprehensive overview of available techniques employed to engineer strains with desired traits. The review highlights both traditional and cutting-edge approaches with a brief introduction to the bacterium's physiology, serving as a foundation for understanding its metabolic capabilities and potential applications. Genome modulation techniques by contrasting traditional methods with CRISPR-based approaches, as well as transcription modulation strategies that enhance gene expression and metabolic flux, and high-throughput techniques that streamline strain development processes are summarized. Furthermore, the roles of artificial intelligence and machine learning in genetic engineering are explored, emphasizing their growing impact on strain development.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phyto-Assisted Synthesis of Silver Nanoparticles (Ag-NPs) Using Delonix elata Extract: Characterization, Antimicrobial, Antioxidant, Anti-Inflammatory, and Photocatalytic Activities.","authors":"Ragavendran Chinnasamy, Arumugam Priyadharsan, Chinnaperumal Kamaraj, Salim Manoharadas, Venkatesan Manigandan, Mukhtar Ahmed, Naushad Ahmad","doi":"10.1007/s12033-025-01449-x","DOIUrl":"https://doi.org/10.1007/s12033-025-01449-x","url":null,"abstract":"<p><p>The aim of present study mainly focuses on the green synthesis of silver nanoparticles (Ag-NPs) utilizing an aqueous extract of Delonix elata, which is an ecologically friendly and sustainable approach. The synthesized Ag-NPs were characterized by UV-visible spectroscopy, FTIR, XRD, SEM with EDAX, TEM and X-ray photoelectron spectroscopy (XPS). A prominent absorption peak at 404 nm in the UV-visible spectrum proved successful nanoparticle synthesis, while FTIR confirmed various functional groups, including methyl, carboxylic acids, and phenolic compounds, involved in the reduction and capping of Ag-NPs. The XRD spectrum shows distinct peaks at 2θ values of 38.02º, 46.15º, 64.41º, and 77.31º, which correspond to the (111), (200), (220), and (311) crystalline planes, respectively. The outcomes validate the existence of Ag-NPs by confirming the face-centered cubic (FCC) shape. SEM and TEM showed spherically arranged particles ranging from 7 to 25 nm. EDAX spectra indicated significant silver content (65.5%) with protein capping. The D. elata produced Ag-NPs had an average zeta potential of - 19.8 mV and a standard variation of 0.8 mV, indicating considerable electrostatic stability. The X-ray photoelectron spectroscopy (XPS) assessment confirmed the production of metallic silver and revealed the surface state makeup of Ag-NPs. Ag-NPs exhibited excellent antibacterial activity, with inhibition zones against E. coli (15.2 mm), P. aeruginosa (17.5 mm), S. mutans (19.2 mm), and S. aureus (12.5 mm). Additionally, the nanoparticles demonstrated strong antioxidant activity, with inhibition rates of 72.55% (ABTS) and 78.23% (DPPH) at 100 μg/mL. Anti-inflammatory tests showed 87% membrane stability in human red blood cells at 100 μg/mL. Additionally, cytotoxicity test against MCF-7 cancer cells revealed an IC₅₀ of 23.43 μg/mL. Treated cells showed significant morphological changes, but no changes were noticed in the control cells. Furthermore, Ag-NPs showed promising photocatalytic activity, achieving 85.3% degradation of methylene blue, highlighting their potential for biomedical and environmental applications.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative Single-Cell and Spatial Transcriptomics Reveal Functional and Spatial Heterogeneity of Atrial and Ventricular Cardiomyocytes in the Heart.","authors":"Lizhi Cao, Rui Chang, Xiaoying Wang, Junwei Shen, Zhifang Yang, Linlin Ma, Yanfei Li","doi":"10.1007/s12033-025-01443-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01443-3","url":null,"abstract":"<p><p>Cardiomyocytes, pivotal for heart contractility, are categorized into atrial (aCM) and ventricular (vCM) subtypes, each playing distinct roles in modulating blood flow, electrical signal conduction, pump function, and energy metabolism. Recent advancements in single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics have enhanced our understanding of cellular heterogeneity and intercellular communication within cardiac tissues. This study integrates scRNA-seq with spatial mapping to elucidate the spatial distribution and intercellular communication of aCM and vCM, focusing on their roles in energy metabolism, pump function, and regulatory functions. We performed scRNA-seq on isolated cardiac cells, followed by data normalization, PCA, and t-SNE clustering, identifying distinct cardiomyocyte subclusters. Ligand-receptor interaction analyses were conducted to explore cellular communication networks, and annotated single-cell data were projected onto heart tissue sections using spatial transcriptomics. Our results revealed distinct spatial distributions: vCM subclusters (vCM-1, vCM-2, vCM-3) predominantly occupied ventricular regions, while aCM subclusters (aCM-1, aCM-2) were primarily located in atrial regions with an increased presence of fibroblasts near atria. Igf2-Igf2r and Vegfb-Vegfr1 mediated communications were prominent in both regions, with extensive interactions between aCM-2 and vCM subclusters. This integration of scRNA-seq and spatial transcriptomics provides a comprehensive overview of cardiac tissue organization and intercellular communication, elucidating critical roles of vCM in energy metabolism and pump function, and aCM in regulating blood flow and electrical conduction. Understanding these interactions in anatomical context enhances our grasp of cardiac function complexity and identifies new therapeutic targets for cardiac diseases.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}