Molecular Biotechnology最新文献_第3页

Correction: Large Clostridial Toxins: A Brief Review and Insights into Antigen Design for Veterinary Vaccine Development. 更正：大型梭状芽孢杆菌毒素：对兽医疫苗开发抗原设计的简要回顾和见解。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-10-01 DOI: 10.1007/s12033-024-01352-x

Rafael Rodrigues Rodrigues, Mariliana Luiza Ferreira Alves, Miguel Andrade Bilhalva, Frederico Schmitt Kremer, Clóvis Moreira Junior, Marcos Roberto Alves Ferreira, Cleideanny Cancela Galvão, Pedro Henrique Dala Nora Quatrin, Fabricio Rochedo Conceição

引用次数: 0

Unlocking Hope: Paving the Way for a Cutting-Edge Multi-Epitope Dengue Virus Vaccine. 开启希望：为尖端的多表位登革热病毒疫苗铺平道路。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-10-01 Epub Date: 2024-10-10 DOI: 10.1007/s12033-024-01294-4

Amtul Wadood Wajeeha, Mamuna Mukhtar, Najam Us Sahar Sadaf Zaidi

{"title":"Unlocking Hope: Paving the Way for a Cutting-Edge Multi-Epitope Dengue Virus Vaccine.","authors":"Amtul Wadood Wajeeha, Mamuna Mukhtar, Najam Us Sahar Sadaf Zaidi","doi":"10.1007/s12033-024-01294-4","DOIUrl":"10.1007/s12033-024-01294-4","url":null,"abstract":"Dengue fever is a significant health issue in Pakistan, demanding a vaccine effective against all the viral strains. This study employs reverse vaccinology to develop potential dengue vaccine candidates (DVAX I-III). The study thoroughly examined conserved areas of dengue virus serotypes 1-4's structural and non-structural proteins. Key viral proteins were analyzed to find antigenic peptides, which were incorporated into vaccine candidates and potentiated with adjuvants. Computational methods predicted peptide structures and evaluated their binding to immune receptors TLR 2, TLR 4, HLA *A1101, and DRB*401. A molecular dynamics simulation lasting 100 ns of the DVAX II-TLR4 complex at different time intervals clearly indicated that the ligand is attached to the receptor. Normal mode analysis assessed the stability and flexibility of these interactions. Encouragingly, all three vaccine candidates demonstrated favorable interactions with these immune receptors and the potential to induce a robust immune response. These findings suggest their safety and warrant further in vivo studies to evaluate their efficacy for clinical development.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3874-3897"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioencapsulation of Transgenic Nannochloropsis sp. Using Artemia as a Vaccine Delivery System to Zebrafish as a Fish Model Against Vibriosis. 利用青蒿作为疫苗递送系统对斑马鱼弧菌病模型进行转基因纳米绿藻的生物包封。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-18 DOI: 10.1007/s12033-025-01496-4

Nur Farhah Nabihan Ismail, Aisamuddin Ardi Zainal Abidin, Fatimah Md Yusoff, Murni Marlina Abd Karim, Ina Salwany Md Yasin, Zetty Norhana Balia Yusof

{"title":"Bioencapsulation of Transgenic Nannochloropsis sp. Using Artemia as a Vaccine Delivery System to Zebrafish as a Fish Model Against Vibriosis.","authors":"Nur Farhah Nabihan Ismail, Aisamuddin Ardi Zainal Abidin, Fatimah Md Yusoff, Murni Marlina Abd Karim, Ina Salwany Md Yasin, Zetty Norhana Balia Yusof","doi":"10.1007/s12033-025-01496-4","DOIUrl":"https://doi.org/10.1007/s12033-025-01496-4","url":null,"abstract":"Aquaculture faces decline due to diseases like vibriosis from Vibrio spp. This study aimed to examined the effect of transgenic microalgae Nannochloropsis sp. expressing the antigen OmpK gene originated from Vibrio harveyi and bioencapsulated with Artemia offers any potential as an oral vaccine in zebrafish. Nannochloropsis sp. was transformed using VCP_OMPK_ZA1 vector and successful transformant was incubated with Artemia prior fed to zebrafish. Zebrafish RNA was extracted to investigate immune response activation via gene expression analysis specifically the IgZ, TNF-ɑ, and IL-1β genes. Vaacinated and Unvaccinated fish were then challenged with pathogenic strain to test the efficacy of the oral vaccine. Results showed IgZ and IL-1β expressions increased on Days 7 and 14, while TNF-ɑ upregulated significantly in transgenic-fed zebrafish compared to wild-type. Upon bacterial challenge, transgenic-fed fish exhibited 100% relative percentage survival (RPS) compared to wild-type-fed (RPS = 40%). The histopathological analysis revealed that the fish that were not vaccinated had severe changes such as hemorrhages and necrosis, whereas those that were vaccinated showed only mild changes such as tubular degeneration and muscle regeneration following the bacterial challenge trial. Results indicate transgenic microalgae enhance larval fish immunity against vibriosis, potentially serving as an effective vaccination method.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

WGCNA-Based Identification of Hub Genes and Key Pathways Involved in Obesity. 基于wgna的肥胖中枢基因和关键通路的鉴定。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-16 DOI: 10.1007/s12033-025-01503-8

Yin Yuan, Shujiao Yue, Zixuan Wu, Xuan Sun, Hongwu Wang

{"title":"WGCNA-Based Identification of Hub Genes and Key Pathways Involved in Obesity.","authors":"Yin Yuan, Shujiao Yue, Zixuan Wu, Xuan Sun, Hongwu Wang","doi":"10.1007/s12033-025-01503-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01503-8","url":null,"abstract":"The prevalence of obesity is increasing year by year, but its characteristic molecular targets are still unclear, and the available therapeutic approaches are relatively limited. Therefore, it is crucial to elucidate the molecular mechanisms underlying the pathogenesis of obesity and to explore potential molecular targets for obesity drug therapy. The expression dataset (GSE73304) was downloaded from the gene expression omnibus database for between-group differential expression gene analyses (DEGs), genome enrichment analysis (GSEA), and weighted gene co-expression network analysis (WGCNA) in healthy and obese populations. Intersecting genes obtained from DEGs and WGCNA difference modules were analyzed with three machine learning methods (LASSO, RandomForest, SVM-REF) to obtain obesity characteristic Genes. Analysis of ROC curves, intergroup differences, and intergene correlations for Genes characterizing obesity. The results of the study showed that 10 specimens and their Gene expression matrices were collected from each of the normal and obese patient groups, yielding 1937 DEGs. GSEA results showed that DEGs were enriched for 32 significant KEGG pathways. Forty gene co-expression modules of the gene expression matrix were constructed by WGCNA. Forty-five intersecting genes were obtained from DEGs and WGCNA significant difference module, which were associated with cellular differentiation, mitochondria, and a variety of endocrine factors and hormones. Eleven genes, including XLOC_004699, RIMBP2, COX6B2, OR5T1, RXFP2, XLOC_003676, XLOC_013038, VAX1, Q07610, XLOC_011515, and PTPN3, were obtained as the obesity characterization Genes through machine learning analysis of intersecting Genes. Based on WGCNA and machine learning, this study found that 11 genes, including RIMBP2, COX6B2, and OR5T1, differed significantly between healthy and obese populations and were closely associated with multiple molecular mechanisms, and these genes may be potential targets for drug therapy and diagnostic biomarkers.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TFAP2A-mediated Transcriptional Activation of ELMO1 Inhibits Ferroptosis and Promotes Esophageal Squamous Cell Carcinoma Progression. tfap2a介导的ELMO1转录激活抑制铁下垂并促进食管鳞状细胞癌进展。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-13 DOI: 10.1007/s12033-025-01511-8

Zhensheng Deng, Jinghong Xu, Zhenguo Liu

{"title":"TFAP2A-mediated Transcriptional Activation of ELMO1 Inhibits Ferroptosis and Promotes Esophageal Squamous Cell Carcinoma Progression.","authors":"Zhensheng Deng, Jinghong Xu, Zhenguo Liu","doi":"10.1007/s12033-025-01511-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01511-8","url":null,"abstract":"The role of engulfment and cell motility protein 1 (ELMO1) in esophageal squamous cell carcinoma (ESCC) is still unknown, even though it is critical for cellular behaviors. Our bioinformatics analyses have predicted transcription factor AP-2 alpha (TFAP2A) as a potential upstream regulator of ELMO1, suggesting its involvement in ESCC progression. ELMO1 expression in ESCC cells was analyzed. Lentivirus-mediated gene silencing was conducted, while cell counting kit-8, wound healing, and transwell assays evaluated the effects of ELMO1 on ESCC cell activities. The transcriptional regulatory effect of TFAP2A on ELMO1 was verified using dual-luciferase reporter assays and ChIP-qPCR. Additionally, ferroptosis-related indicators were detected to explore the potential role of TFAP2A/ELMO1 in ESCC. A nude mouse xenograft model was established to analyze tumor growth in vivo. ELMO1 was upregulated in KYSE150 cells. Silencing of ELMO1 suppressed ESCC cell migration and invasion, while sensitizing cells to ferroptosis. TFAP2A transcriptionally activated ELMO1 by binding to its promoter, thereby enhancing ESCC cell invasive potential. In vivo, TFAP2A knockdown activated ferroptosis and inhibited tumor growth, whereas ELMO1 overexpression promoted tumor progression. TFAP2A facilitates ESCC cell proliferation, migration, and invasion by promoting ELMO1 transcription and inhibiting ferroptosis. Both TFAP2A and ELMO1 act as oncogenic drivers in ESCC and may represent potential therapeutic targets.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145054666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Data-Driven Discovery of a Key Regulator Cyclin A2 as a Promising Therapeutic Target in Hormone-Sensitive Cancers. 数据驱动的关键调节因子细胞周期蛋白A2作为激素敏感性癌症的有希望的治疗靶点的发现。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-11 DOI: 10.1007/s12033-025-01504-7

Suvitha Anbarasu, Anand Anbarasu

{"title":"Data-Driven Discovery of a Key Regulator Cyclin A2 as a Promising Therapeutic Target in Hormone-Sensitive Cancers.","authors":"Suvitha Anbarasu, Anand Anbarasu","doi":"10.1007/s12033-025-01504-7","DOIUrl":"https://doi.org/10.1007/s12033-025-01504-7","url":null,"abstract":"Hormone-sensitive cancers (HSCs) are one of the predominant types of cancer leading to death globally. The current study has attempted to discover a potential therapeutic target that could be used against HSCs in women, namely, breast, ovarian, and endometrial cancer. The differentially expressed genes in each cancer type were compared with previously reported tamoxifen resistance-causing genes. The hub genes CCNA2, CDCA8, ISG15, and E2F1 were found in breast cancer, CCNA2, CDCA8, CXCR4, and LYN were found in ovarian cancer, and CCNA2 and POLE2 were found in endometrial cancer. The clusters with the hub genes were screened for functional importance and were found to be significant in cell cycle regulation pathways. The expression significance, correlation, mutational profile, survival potency, treatment response status, and clinical profile revealed that CCNA2 was significantly associated with all three cancers. This study revealed that CCNA2 was positively correlated with other resistance-causing genes such as CENPE, MK167, CDCA8, NEK2, PRC1, ZWINT, CDKN3, MYBL2, and E2F1, in various cancers, validating its potential to cause resistance. Compared with other hub genes, CCNA2 had a Lower mutation percentage and critical hazard ratios of 2.16, 1.75, and 1.68 in breast, ovarian, and endometrial cancers, respectively, indicating its pivotal role in survival. The median CCNA2 expression level was 21.301 in stage IV BC patients, 38.481 in stage II ovarian cancer patients, and 23.206 in stage IV endometrial cancer patients. Thus, CCNA2 could be a potential therapeutic target for treating HSCs with endocrine therapy resistance.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase. 巴贝斯虫乳酸脱氢酶的表达、纯化及生化特性研究。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-10 DOI: 10.1007/s12033-025-01500-x

Safiye Merve Bostancioglu, Ozkan Danis, Sinem Kocer, Ozal Mutlu

{"title":"Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase.","authors":"Safiye Merve Bostancioglu, Ozkan Danis, Sinem Kocer, Ozal Mutlu","doi":"10.1007/s12033-025-01500-x","DOIUrl":"https://doi.org/10.1007/s12033-025-01500-x","url":null,"abstract":"Babesia bigemina, a tick-borne protozoan parasite, is one of the main causative agents of bovine babesiosis, a disease with significant economic impact on the cattle industry. One of the key enzymes involved in the parasite's metabolism is lactate dehydrogenase (LDH), which plays an essential role in the anaerobic glycolytic pathway by catalysing the conversion of pyruvate to lactate. In this study, B. bigemina LDH gene was cloned, expressed in Escherichia coli and subsequently purified using affinity chromatography. The purified enzyme was subjected to biochemical assays to determine its stability, optimal pH, thermostability, and kinetic parameters. Kinetic analyses indicated a Kₘ value of 0.2585 mM for pyruvate and a K0.5 value of 0.3094 mM for NADH. B. bigemina LDH exhibits typical Michaelis-Menten kinetics for pyruvate, but its behavior towards NADH is similar to that of Cryptosporidium parvum LDH, suggesting that B. bigemina LDH may function as an allosteric enzyme for NADH. Enzyme activity was found to remain stable for up to 9 days at 4 °C without any preservative agent. Biochemical analysis showed that the optimum enzymatic activity occurred at pH 8.5, and the enzyme retained its activity at both 30 °C and 40 °C. These findings provide valuable insights into the functionality of the enzyme and may contribute to the development of therapeutic interventions targeting the glycolytic pathway of B. bigemina.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimising Guide RNA Production for Multiplexed Cas9-Targeted Nanopore Sequencing to Detect Pathogens. 优化引导RNA生产用于多重cas9靶向纳米孔测序检测病原体。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-07 DOI: 10.1007/s12033-025-01510-9

Gus R McFarlane, Kim Whitaker, Krista L Plett, Brendon O'Rourke, Daniel R Bogema

{"title":"Optimising Guide RNA Production for Multiplexed Cas9-Targeted Nanopore Sequencing to Detect Pathogens.","authors":"Gus R McFarlane, Kim Whitaker, Krista L Plett, Brendon O'Rourke, Daniel R Bogema","doi":"10.1007/s12033-025-01510-9","DOIUrl":"https://doi.org/10.1007/s12033-025-01510-9","url":null,"abstract":"In agriculture, biosecurity, and human health, the rapid and accurate detection of pathogens and pests is crucial. Our study investigates the sensitivity and practicality of six guide RNA (gRNA) production methods for use in Nanopore Cas9-targeted sequencing (nCATS), focusing on their implications for multiplexed pathogen and pest detection. Each production method generated a library of eight gRNAs capable of excising ~ 1.6 kb fragments of the 5.8S_rRNA-ITS2-28S_rRNA regions of five economically significant wheat fungal pathogens. Through comparative analysis, we evaluated the efficacy of commercially synthesised and in-house in vitro-transcribed gRNAs, assessing their impact on sequencing enrichment outcomes. Our findings highlight differences amongst the methods in terms of gRNA yield, integrity, performance, and costs. Our best performing gRNA production method was able to successfully identify all target sequences across the 0.96 to 8.4 pg ranges we tested with coverage ranging from 66 to 2037X. This study highlights the challenges and opportunities in implementing nCATS for multiplexed pathogen and pest detection, offering insights into the development of cost-effective and reliable gRNA production strategies for nCATS.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study of Transfection Techniques for Genetic Modification in Chicken Primordial Germ Cells. 鸡原始生殖细胞基因改造转染技术的比较研究。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-05 DOI: 10.1007/s12033-025-01501-w

Lu Meng, Zimo Zhu, Man Xue, Feiyi Wang, Yuxiao Ma, Guiyu Zhu

{"title":"A Comparative Study of Transfection Techniques for Genetic Modification in Chicken Primordial Germ Cells.","authors":"Lu Meng, Zimo Zhu, Man Xue, Feiyi Wang, Yuxiao Ma, Guiyu Zhu","doi":"10.1007/s12033-025-01501-w","DOIUrl":"https://doi.org/10.1007/s12033-025-01501-w","url":null,"abstract":"Avian species possess a distinct reproductive system that differs fundamentally from mammals, resulting in relatively slower progress in transgenic poultry research compared to mammalian models. Primordial germ cells (PGCs), as the precursors to functional gametes, provide a promising avenue for the utilization of genetic resources and the application of transgenesis in chickens. However, the transfection of chicken PGCs remains challenging due to the low efficiency of conventional methods. This study systematically compared the transient transfection efficiency of chicken PGCs using lipofection, electroporation, adenovirus (ADV), and adeno-associated virus (AAV) to identify the optimal genes delivery approach. Lipofection exhibited less than 5% efficiency at 24 h, whereas electroporation significantly increased transfection efficiency but reduced cell viability at 48 h. Adenoviral vectors achieved the highest delivery efficiency 8-10 fold greater than lipofection, surpassing both lipid-mediated transfection and electroporation, while adeno-associated viruses serotypes showed minimal infectivity in PGCs. Furthermore, ADV-transduced PGCs retained normal migratory capability after transplantation into 2.5-day chicken embryos, confirming that adenoviral infection did not impair their biological functionality. Collectively, our findings established adenoviral delivery as a robust and efficient method for gene transfer in chicken PGCs, enabling precise genome engineering for applications in transgenic avians production and the conservation of endangered poultry genetic resources.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hamamelitannin from Hamamelis virginiana Attenuates Ethanol-Induced Oxidative and Inflammatory Responses in Danio rerio Larvae. 维吉尼亚金缕梅中的金缕梅单宁能减弱乙醇诱导的丹尼氏幼虫的氧化和炎症反应。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-04 DOI: 10.1007/s12033-025-01502-9

Vishnu Adith Janarthanam, Panneer Selvam Sundar Rajan, Siva Prasad Panda, Uttam Prasad Panigrahy, Rupesh Gupta, Ajay Guru, Praveen Kumar Issac

{"title":"Hamamelitannin from Hamamelis virginiana Attenuates Ethanol-Induced Oxidative and Inflammatory Responses in Danio rerio Larvae.","authors":"Vishnu Adith Janarthanam, Panneer Selvam Sundar Rajan, Siva Prasad Panda, Uttam Prasad Panigrahy, Rupesh Gupta, Ajay Guru, Praveen Kumar Issac","doi":"10.1007/s12033-025-01502-9","DOIUrl":"https://doi.org/10.1007/s12033-025-01502-9","url":null,"abstract":"Alcoholic liver disorder (ALD) is one of the most prevalent hepatic ailments worldwide, with oxidative stress and inflammation playing a vital role in disease progression. The current study intended to assess the anti-inflammatory nature of Hamamelitannin (HAM), a gallotannin from Hamamelis virginiana barks, which was predicted to possess anti-inflammatory properties based on in-silico docking analysis. To further explore its effects, we examined the therapeutic effect of HAM against ethanol-mediated inflammation using an in-vivo zebrafish larvae model. Ethanol exposure led to liver inflammation, oxidative stress, lipid accumulation, and hepatocyte apoptosis. However, our findings demonstrated that co-treatment with HAM significantly normalized the larvae's antioxidant enzymes such as SOD (35.81 U/mg protein), CAT (33.83 μ mol/mg protein) and GPx (33.35 U/mg Protein), nitric oxide (NO), lipid accumulation, reactive oxygen species (19.9%), cell death (15.43%), LPO (17.4%), and macrophage infiltration. A gene expression analysis was performed to gain deeper insights into ethanol-induced hepatotoxicity and the protective role of HAM. The results revealed that ethanol exposure led to the upregulation of Inflammation-inducing markers, including iNOS, TNF-α, COX-2, and IL-1β. In contrast, HAM co-treatment mitigated hepatocyte damage by effectively downregulating these inflammatory mediators. Collectively, these findings suggest that HAM exhibits promising hepatoprotective and anti-inflammatory properties, indicating its therapeutic potential for ALD and other inflammation-driven ailments.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0