Molecular Biotechnology最新文献

{"title":"Retraction Note: Triamcinolone Acetonide Produced by Bacillus velezensis YEBBR6 Exerts Antagonistic Activity Against Fusarium oxysporum f. sp. Cubense: A Computational Analysis.","authors":"R U Krishna Nayana, S Nakkeeran, N Saranya, R Saravanan, K Mahendra, Suhail Ashraf, Kahkashan Perveen, Najla A Alshaikh, R Z Sayyed, Pau Loke Show","doi":"10.1007/s12033-026-01614-w","DOIUrl":"https://doi.org/10.1007/s12033-026-01614-w","url":null,"abstract":"","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149183132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis of Milk Protein Gene Expression Regulation Using CiteSpace and VOSviewer.","authors":"Xiaoliang He, Lili Xing, Linjie Shi, Xiaohui Zhou","doi":"10.1007/s12033-026-01609-7","DOIUrl":"https://doi.org/10.1007/s12033-026-01609-7","url":null,"abstract":"<p><p>Milk proteins represent the principal protein constituents of mammalian milk and play indispensable biological roles in the growth, development, and immune maturation of neonates. Modulating the expression of genes involved in milk protein synthesis holds considerable promise for enhancing both the efficiency of milk protein production and the bioavailability of dairy-derived nutrients, thereby offering significant translational potential in neonatal nutrition and the functional optimization of bioactive milk peptides. This study conducts a comprehensive bibliometric analysis of the scientific literature on milk protein gene expression regulation, drawing upon publications indexed in the Web of Science Core Collection from 2006 to 2025. Utilizing CiteSpace and VOSviewer, we systematically map research hotspots, evolutionary trends, and emerging frontiers in the field. Concurrently, we provide an integrative review of the molecular mechanisms governing milk protein gene expression, including key transcriptional regulators and associated signaling pathways, as well as recent advances in the optimization and innovative applications of milk protein-based products and pharmaceuticals.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pentavalent Recombinant Vaccine Using Escherichia coli Bacterins Against Major Clostridium Toxins in Ruminants.","authors":"Fabricio Rochedo Conceição, Rafael Rodrigues Rodrigues, Jaqueline Freitas Motta, Clóvis Moreira Júnior, Mariliana Luiza Ferreira Alves, Elcidimar Lucas Aleixo de Castro, Fernanda Monik Silva Martins, Welington Mateus Pinto de Moraes, Cristian Henrique Sott, Felipe Masiero Salvarani, Marcos Roberto Alves Ferreira","doi":"10.1007/s12033-026-01610-0","DOIUrl":"https://doi.org/10.1007/s12033-026-01610-0","url":null,"abstract":"<p><p>Clostridium perfringens and Clostridium botulinum are anaerobic spore-forming bacteria that produce potent toxins and are major causative agents of severe toxicoinfections in ruminants. Although vaccination remains the primary preventive strategy, current commercial vaccines are based on crude toxoid preparations, which present several limitations, including labor-intensive manufacturing processes, high biological risk, and batch-to-batch variability. This study aimed to evaluate the immunogenicity of a pentavalent recombinant bacterin vaccine formulation containing antigens derived from C. perfringens (CPA, CPB, and ETX) and C. botulinum (BoNT/C and BoNT/D) in cattle, sheep, goats, and buffaloes. Individual and chimeric recombinant antigens were expressed in Escherichia coli and incorporated into a vaccine formulation containing three inactivated bacterial components (bacterins) adsorbed onto aluminum hydroxide. A total of 92 ruminants (23 cattle, 23 buffaloes, 23 sheep, and 23 goats) were included in the study and allocated to receive the recombinant vaccine, a commercial vaccine, or saline as a negative control. Neutralizing antibody titers were determined by serum neutralization assays. The recombinant vaccine was well tolerated across all species and induced neutralizing antibody titers of 10 IU/mL against CPB, 5 IU/mL against BoNT/C, and 4 IU/mL against BoNT/D. The response to ETX was species-dependent, with cattle and sheep reaching antibody titers of 4 IU/mL, whereas buffaloes and goats reached 2 IU/mL. Neither the recombinant bacterin nor the commercial vaccine elicited detectable neutralizing antibody responses against CPA, highlighting the need for further strategies to improve the immunogenicity of this antigen. Nevertheless, the overall findings support the potential of the pentavalent recombinant vaccine to confer protective immunogenicity against multiple clostridial toxins. Incorporating recombinant nontoxic antigenic domains into a bacterin-based platform represents a safe, scalable, and antigen-sparing alternative to conventional toxoid vaccines, with promising implications for improving the prevention and control of clostridial diseases in ruminant livestock.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-Wide Identification and Expression Analysis of Kinase Proteins in Land Plants and Their Expression in Gossypium hirsutum Under Salt Stress.","authors":"Aquib Nazar, Athar Hussain, Muhammad Asim Ali, Adnan Shoukat, Naila Ali, Zaib-Un-Nisa","doi":"10.1007/s12033-026-01613-x","DOIUrl":"https://doi.org/10.1007/s12033-026-01613-x","url":null,"abstract":"<p><p>The kinase proteins are a superfamily in plants and are involved in diverse biological and molecular functions for better adaptation of land plants. Protein kinase diversification is hypothesized to enhance plant adaptation to abiotic stress, particularly salinity. This study tests whether evolutionary expansions of stress-responsive kinase subfamilies correlate with functional variation in salt tolerance. The study identified a total of 49,611 genes in 32 land plants across mosses, bryophytes, lycophytes, gymnosperms, and angiosperms. All identified genes were classified into 26 major classes (I-XXVI) based on domain architectures, e.g., protein kinase tyrosine (PkT), leucine-rich repeat (LRR), LRR-protein kinases (LRR-Pk), LRR-Pk tyrosine kinases (LRR-PkT), and protein kinase (Pk) and the X domain representing other than the listed domains. RNA-seq-based expression profiling under biotic and abiotic stresses and in various plant tissue of Arabidopsis thaliana (Ath), Glycine max (Gma), Gossypium hirsutum (Ghi), Zea mays (Zma), and Oryza sativa (Osa) highlighted potential OGs including OG₁₂, OG₃₅, and OG₄₄ showing response in all plants. Furthermore, upregulation of OG₁₂_LR_GhPk08 in Ghi under both biotic, (e.g., Xanthomonas citri) and abiotic (e.g., salt, heat, and cold) stress, as well as in specific tissues like roots and seedlings, suggest a potential role in stress responses. Molecular docking analysis demonstrated the preferential binding of ATP to Ghi kinase proteins, indicating their conserved phosphorylation mechanisms. Furthermore, SNP and InDel analyses in salt-resistant (Mac7) and salt-susceptible (Coker 312) cotton genotypes revealed genetic variations potentially linked to differential stress responses. Moreover, the qRT-PCR-based expression analysis of two genes (Gohir.D08G10000; OG₁₂_LR_GhPk08, Gohir.D05G155340; OG₃₅_Pk_GhPk02) out of three, showed salt-responsive transcription under the tested condition in Mac7 and Coker 312 cotton genotypes, supporting their prioritization as candidate genes for further functional validation and characterization. Thus, the study provides a deep insight into diversity and functional responses of kinase genes in land plants, particularly, in cotton leading to future research on kinase studies.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCR-LbCas12a Assay for Genotyping of HPV 16 and 18.","authors":"ChonIl Paek, Jin Zhou, HuiYon Sin","doi":"10.1007/s12033-026-01612-y","DOIUrl":"https://doi.org/10.1007/s12033-026-01612-y","url":null,"abstract":"<p><p>CRISPR/Cas12a systems have been widely used in nucleic acid detection, because of their high specificity and trans-cleavage activity. We combined the specificity of CRISPR/Cas12a system with the sensitivity of PCR, to develop a new HPV detection assay with high specificity and sensitivity, called PCR-LbCas12a assay. Here, we pre-amplified almost all high-risk HPV genotypes with consensus primer (MY09/11) and added to new tubes containing HPV 16 or 18 CRISPR/Cas12a system for HPV genotyping. The LOD of PCR-LbCas12a for HPV16 or HPV18 DNA was 100 copies and 10 copies per reaction, respectively. In mixed samples of HPV 16 and HPV 18 DNA, PCR-LbCas12a assay could detect HPV16 DNA from samples containing 10² copies of HPV16 and 10⁴ copies of HPV18. On the other hand, HPV18 DNA was detected from samples containing 10³ copies of HPV16 and HPV18, respectively. Following validation with HPV-positive and HPV-negative cell lines (SiHa, HeLa, and C-33A), the PCR-LbCas12a assay was evaluated using 39 clinical samples previously characterized by type-specific qPCR. The assay demonstrated a sensitivity of 91.3 (95 CI 71.9-98.9) and a specificity of 100. The PCR-LbCas12a assay can discriminate the HPV 16 and HPV 18 genotypes, and holds great potential in simultaneous detection of most high-risk HPV genotypes including HPV 16 and HPV18.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148982780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantifying HER-2 for Precision Oncology: The Role of Optical Biosensors.","authors":"Qamar Abuhassan, Jamal I Al-Nabulsi, Pareshkumar N Patel, K V Jamuna, Vandana Tripathi, Priya Priyadarshini Nayak, Navin Kumar Tailor, Dildora Turaeva","doi":"10.1007/s12033-026-01611-z","DOIUrl":"https://doi.org/10.1007/s12033-026-01611-z","url":null,"abstract":"<p><p>The ability to measure the Human Epidermal Growth Factor Receptor 2 (HER-2), a biomarker associated with aggressive breast cancer and other malignancies, is vital to both providing an accurate diagnosis and tailoring individualized therapeutic management of patients. Although traditional approaches to diagnose HER-2 through laboratory testing using immunohistochemical techniques are established methods, they may be slow, semi-quantitative, and dependent on centralized laboratory facilities. This review first provides a concise overview of the biomarker HER-2 and its role in cancer, then proceeds to review recent advances in optical biosensing platforms designed for the sensitive and specific detection of HER-2. We describe how various optical transducers, including fluorescence, surface plasmon resonance (SPR), and colorimetric assays, are being used for quantification of HER-2 in clinical and experimental settings. We also highlight emerging point-of-care (POC) devices that utilize optical technologies for rapid, decentralized HER-2 measurement. In conclusion, this review discusses current challenges associated with the clinical translation of optical biosensors, including standardization, large-scale validation, and regulatory implementation, while also highlighting future directions involving nanotechnology integration, liquid biopsy strategies, and AI-assisted diagnostic systems for precision oncology.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148956210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological Profile and Antimicrobial Resistance Patterns of Pleural Fluid Isolates in a Tertiary Care Setting in Lahore, Pakistan.","authors":"Hassan Imran, Zaman Khan, Fatima Iftikhar Shah, Fatima Akram, Fiza Saleem, Somia Shehzadi","doi":"10.1007/s12033-026-01574-1","DOIUrl":"10.1007/s12033-026-01574-1","url":null,"abstract":"<p><p>Pleural fluid infections, primarily caused by a range of bacterial pathogens, present a growing challenge for healthcare professionals, particularly due to the increasing prevalence of antibiotic resistance. This study included 598 patients, of which 15.88% had culture-positive samples. A total of 18 distinct bacterial species, both individual and in combination, were identified, with 7 Gram-positive and 11 Gram-negative bacteria. Antibacterial susceptibility testing highlighted colistin as the most effective antibiotic for most Gram-negative pathogens, while vancomycin and linezolid showed increased sensitivity against Gram-positive isolates. The study calculated the Multiple Antibiotic Resistance (MAR) index for all isolates, yielding a mean value of 0.543, reflecting the high level of irrational antibiotic use. Resistance pattern analysis revealed significant variation among bacterial species and identified 61 distinct resistance profiles. The study also examined the relationship between the MAR index and factors such as gender, age, and Gram staining. Gender (p = 0.831) and age (p = 0.905) showed no significant correlation with the MAR index, while a significant negative correlation was found between Gram staining and the MAR index (ρ = - 0.434, p < 0.001). The findings highlight the increasing prevalence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) isolates, presenting significant challenges for treatment, particularly in resource-limited settings. This underscores the urgent need for enhanced antibiotic stewardship, public awareness campaigns, and continuous monitoring to combat antibiotic resistance and improve patient outcomes. Furthermore, the study's findings contribute to global efforts in understanding and addressing the escalating problem of antibiotic resistance, offering insights relevant to international research on antimicrobial resistance.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3727-3745"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147618785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tropical Seaweeds as a Sustainable Resource Towards Circular Bioeconomy: Insights and Way Forward.","authors":"Phei Er Kee, Siew Moi Phang, John Chi-Wei Lan, Joo Shun Tan, Kuan Shiong Khoo, Jo-Shu Chang, Hui-Suan Ng","doi":"10.1007/s12033-023-00940-7","DOIUrl":"10.1007/s12033-023-00940-7","url":null,"abstract":"<p><p>Seaweeds are photosynthetic marine macroalgae known for their rapid biomass growth and their significant contributions to global food and feed production. Seaweeds play a crucial role in mitigating various environmental issues, including greenhouse gases, ocean acidification, hypoxia, and eutrophication. Tropical seaweeds are typically found in tropical and subtropical coastal zones with warmer water temperatures and abundant sunlight. These tropical seaweeds are rich sources of proteins, vitamins, minerals, fibers, polysaccharides, and bioactive compounds, contributing to their health-promoting properties and their diverse applications across a range of industries. The productivity, cultivability, nutritional quality, and edibility of tropical seaweeds have been well-documented. This review article begins with an introduction to the growth conditions of selected tropical seaweeds. Subsequently, the multifunctional properties of tropical seaweeds including antioxidant and anti-inflammatory, anti-coagulant, anti-carcinogenic and anti-proliferative, anti-viral, therapeutic and preventive properties were comprehensively evaluated. The potential application of tropical seaweeds as functional foods and feeds, as well as their contributions to sustainable cosmetics, bioenergy, and biofertilizer production were also highlighted. This review serves as a valuable resource for researchers involved in seaweed farming as it provides current knowledge and insights into the cultivation and utilization of seaweeds.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3554-3571"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-181a-5p promotes papillary thyroid carcinoma progress via the PTEN/AKT pathway.","authors":"Wenyu Zhao, Yiting Guo, Liang He, Junhong Li, Zhenglun Qiu, Haojie Chen, Leilei Li, Hua Wang, Yunlong Wang, Yanfang Zhang","doi":"10.1007/s12033-026-01581-2","DOIUrl":"10.1007/s12033-026-01581-2","url":null,"abstract":"<p><p>The objective of this investigation was to determine the expression profile and latent mechanism of microRNA-181a-5p (miR-181a-5p) in the genesis and progression of papillary thyroid cancer (PTC). MiR-181a-5p was discovered to be upregulated in PTC tissues and cells in this study, as confirmed by RT‒qPCR and The Cancer Genome Atlas database. Notably, in PTC patients, the miR-181a-5p level was linked to tumor size and thyroid capsule invasion. A series of experiments demonstrated that miR-181a-5p upregulation in PTC cells notably enhanced proliferation, motility, and invasion, whereas suppressing miR-181a-5p hindered these functions. Western blotting revealed that miR-181a-5p suppressed PTEN expression, boosting the activation of phosphorylated AKT (P-AKT). According to predictive bioinformatics research and luciferase reporter gene tests, miR-181a-5p may target a specific binding site on the PTEN 3'UTR. To sum up, this study indicated that miR-181a-5p promoted PTC progression through the PTEN/Akt pathway. This investigation reveals a potential mechanism for PTC progression and provides a foundation for clinical therapies.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3715-3726"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13558409/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal Effect on Algae, Biofilm and Their Composition Towards Membrane Distillation Unit: A Mini-review.","authors":"Yin Sim Ng, Derek Juinn Chieh Chan","doi":"10.1007/s12033-023-00853-5","DOIUrl":"10.1007/s12033-023-00853-5","url":null,"abstract":"<p><p>Membrane distillation (MD) has lower operating temperature and potential to recycle waste heat for desalination which catches much attention of the researchers in the recent years. However, the biofouling is still a challenging hurdle to be overcome for such applications. The microbial growth rate, secretion and biofilm formation are sensitive to heat. Membrane distillation is a thermally driven separation, so the increase of temperature in the seawater feed could influence the extent of biofouling on the unit parts. In this review, we present the effect of temperature on algal growth, the range of temperature the microbes, marine algae and planktons able to survive and the changes to those planktons once exceed the critical temperature. Thermal effect on the biofilm, its composition and properties are discussed as well, with association of the biofilm secreting microbes, but the study related to membrane distillation unit seems to be lacking and MD biofouling factors are not fully understood. Characterization of the algae, biofilm and EPS that govern biofouling are discussed. This information not only will help in designing future studies to fill up the knowledge gaps in biofouling of membrane distillation, but also to some extent, assist in pointing out possible fouling factors and predicting the degree of biofouling in the membrane distillation unit.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3512-3522"},"PeriodicalIF":3.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10495708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}