Molecular Biotechnology最新文献

{"title":"VHI-Pred: A Multi-Feature-Based Tool for Predicting Human-Virus Protein-Protein Interactions.","authors":"Rasool Sahragard, Masoud Arabfard, Ali Ahmadi, Ali Najafi","doi":"10.1007/s12033-025-01417-5","DOIUrl":"https://doi.org/10.1007/s12033-025-01417-5","url":null,"abstract":"<p><p>Viral diseases pose a significant threat to public health, highlighting the importance of understanding protein-protein interactions between hosts and viruses for therapeutic development. However, this process is often expensive and time-consuming, especially given the rapid evolution of viruses. Machine learning algorithms and artificial intelligence have emerged as powerful tools for efficiently identifying these interactions. This study aims to develop a machine learning-based model to predict protein interactions between viral pathogens and human hosts while analyzing the factors influencing these interactions. The prediction model was constructed using three machine learning algorithms: Random Forest (RF), XGBoost (XGB), and Artificial Neural Networks (ANN). Each algorithm underwent rigorous testing. The modeling features included physicochemical properties, motifs, and amino acid sequences. Model performance was evaluated using fitness, accuracy, precision, sensitivity, and specificity metrics, with validation conducted via the K-fold method. The accuracy of the RF, XGB, and ANN models was 87%, 86%, and 86%, respectively. By integrating dimensionality reduction and clustering techniques, the accuracy of the RF model improved to 90%. Traditionally, studying host-pathogen interactions is labor intensive and costly. The integration of machine learning algorithms into this field significantly enhances the efficiency of analyzing viral pathogen-human host interactions. This study demonstrates the effectiveness of such an approach and provides valuable insights for future research. The results are accessible to researchers through a web application at http://vhi.sysbiomed.ir .</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editing gliA, gliP and gliZ of Aspergillus fumigatus Using CRISPR/Cas System Renders Fungus Incapable to Produce Gliotoxin.","authors":"Rida Zainab, Arsh Mukhtar, Zakia Saleem, Haiba Kaul, Ali Ahmad, Muhammad Majeed","doi":"10.1007/s12033-025-01429-1","DOIUrl":"https://doi.org/10.1007/s12033-025-01429-1","url":null,"abstract":"<p><p>Aspergillus fumigatus is a saprophytic fungus that causes respiratory infections in human, animals, and birds. This fungus produces gliotoxin which is a secondary metabolite that triggers pathogenicity. Gliotoxin is encoded by a 13-gene cluster including gliA, gliP and gliZ. The purpose of this study was to determine whether the fungus produces gliotoxin after these genes are edited using CRISPR/Cas system. For this, crRNAs for gliA, gliP and gliZ were designed using EuPaGDT, while tracrRNA and Cas9 protein were purchased ready-made. These crRNAs were individually annealed with the tracrRNA to make three gRNAs which were then individually combined with the Cas9 to make three ribonucleoprotein (RNP) complexes. A. fumigatus protoplasts were enzymatically generated and transfected with each of the RNP complexes (group 1) in PEGylated conditions. Non-treated protoplasts were simultaneously run as control (group 2). Transfected protoplasts showed reduced growth on SDA plates as compared to their control. Gliotoxin extraction through thin-layer chromatography was carried out for both the groups which showed the absence of gliotoxin in group 1. Sequencing results confirmed the indels in target genes which shows that the CRISPR/Cas9 system effectively targeted A. fumigatus' gliotoxin-related genes that rendered fungus incapable to produce gliotoxin. This work may pave the way to develop effective strategies to control the infections caused by A. fumigatus.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Soil Microbiomes on Mung Bean Cultivation: Insights from 16S rRNA Metagenomics.","authors":"Karuppasamy Saveetha, Chaitanya Sree Somala, Thirunavukarasou Anand, Dhanushkodi Balamurugan, Venkatachalam Vasudevan, Konda Mani Saravanan, Renganathan Senthil","doi":"10.1007/s12033-025-01425-5","DOIUrl":"https://doi.org/10.1007/s12033-025-01425-5","url":null,"abstract":"<p><p>Cyclic nutrient processes, soil health maintenance, and plant development are contingent upon soil microbiomes. The microbial makeup of the soil of Maruthupandiyar College, Thanjavur, is assessed using 16S rRNA gene sequencing. QIIME2, in conjunction with the SILVA database, analyzed the sequencing data to examine microbial diversity and composition. The experimental results revealed a diverse array of bacteria in soil physicochemical properties. The alpha and beta diversity assessment revealed significant microbial community complexity and distribution patterns disparities. The research revealed bacterial groups associated with biological nitrogen fixing, suggesting their potential to enhance mung bean growth. The current study illustrates the significance of microbial interactions in soil for sustaining soil fertility and enhancing crop output. Research findings provide essential insights into improving the sustainability of tropical agriculture through intentional microbial management to create sustainable soil health systems.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly Biocontrol of Ralstonia solanacearum and Plant Growth Promotion in Tobacco Using Garbage Enzyme and Bacillus velezensis A1.","authors":"Syed Sib Tul Hassan Shah, Wangjie Shan, Ying Wang, Zhisheng Zheng, Shuo Feng, Lingxiao Wang, Xiufang Hu, Lin Li","doi":"10.1007/s12033-025-01412-w","DOIUrl":"https://doi.org/10.1007/s12033-025-01412-w","url":null,"abstract":"<p><p>Ralstonia solanacearum, the causative agent of bacterial wilt disease, poses a significant threat to agriculture. The demand for safe and high-quality food has increased interest in biological control agents (BCAs), despite challenges related to stability and cost. Garbage enzymes (GEs) are a promising alternative, rich in beneficial microbes and organic compounds. In this study, four types of GEs (onion, garlic, ginger, and mixed fruit) were individually tested against R. solanacearum in vitro, with only onion GE showing significant inhibition. Filtered onion GE, which lacked any microbes, also showed inhibition; however, its heat-treated form exhibited a reduced inhibitory effect, indicating the role of heat-sensitive compounds in inhibiting R. solanacearum. An antagonistic bacterial strain A1, isolated from onion GE, inhibited pathogen growth by up to 75% through volatile compounds. Cell-free culture filtrate of the strain A1 also inhibited R. solanacearum in vitro. Strain A1 exhibited nitrogen fixation, siderophore production, indole acetic acid (IAA) synthesis, and extracellular enzyme production, positioning it as a potent biocontrol agent. The genome analysis of the strain A1 revealed the presence of several plant growth-promoting genes. In vivo studies with GE, GE-filtered, and strain A1 demonstrated significant pathogen inhibition and promoted tobacco plant growth. Disease incidence was reduced to 26.6% with GE, 46.67% with microbe-free GE, and 40% with strain A1. Overall, these treatments positively impacted plant root and shoot lengths as well as both fresh and dry weights. Our findings highlight onion GE as a potential, environmentally friendly method for controlling bacterial wilt and enhancing plant development, offering an alternative approach to traditional chemical controls in agriculture.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAX as a Biomarker for Predicting Immunotherapeutic Efficacy in Uveal Melanoma Patients: A Comprehensive Analysis.","authors":"Yao Shi, Changjiang Lei, Hong Jiang, Yan Hong, Wei Su, Shanxia Wu, Xiaobo Yang","doi":"10.1007/s12033-025-01395-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01395-8","url":null,"abstract":"<p><p>Uveal melanoma (UVM) is the second most common type of malignant melanoma occurring in the eye, which arises from the interstitial melanocytes in the uveal tract. This study aims to identify a highly efficient biomarker for the immunotherapy against UVM. Initially, a comprehensive analysis was conducted using the transcriptional and clinical data from The Cancer Genome Atlas (TCGA) database through the immune and stromal scores to assess the composition of infiltrating immune cells in the tumor microenvironment. Further, the expression of BCL2-Associated X, Apoptosis Regulator (BAX), and its co-expression gene networks were analyzed using the weighted gene co-expression network analysis (WGCNA) to identify relevant gene modules and hub genes. The immunohistochemistry (IHC) analysis was carried out to confirm the influence of BAX on immune infiltration. In addition, the survival analysis on the hub genes, including BAX, was performed using an external dataset from the Gene Expression Omnibus (GEO) to corroborate the prognostic significance of these genes in an independent patient cohort. A nomogram integrating patients' clinical features was developed to predict the survival outcomes. Our investigations revealed that high BAX expression was associated with severe clinical characteristics and poor prognosis in UVM. Our analyses identified 12 hub genes at the intersection of differentially expressed genes categorized by BAX expression levels and a co-expression gene model. Further, the GEO database validated the prognostic significance of these hub genes. The IHC analysis established a significant correlation between BAX expression and immune infiltration. This nomogram model demonstrated robust predictive efficiency with a concordance index (C-index) of 0.909 (95% CI: 0.846-0.971), indicating excellent discriminative ability. The calibration curves for 1-year, 3-year, and 5-year overall survival (OS) rates confirmed the nomogram's accuracy, closely reflecting the actual patient outcomes. Finally, the Decision Curve Analysis (DCA) revealed that this nomogram could accurately predict OS for a majority of patients, covering a probability range of 25-95%. Our research may provide a new therapeutic regimen to benefit the UVM patients.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Multi-omics and Mendelian Randomization Reveal the Key Role of Monocytes in Aging and Osteoarthritis.","authors":"Xiangwen Shi, Linmeng Tang, Mingjun Li, Mingjie Wei, Yipeng Wu, Yongqing Xu","doi":"10.1007/s12033-025-01416-6","DOIUrl":"https://doi.org/10.1007/s12033-025-01416-6","url":null,"abstract":"<p><p>There is a close connection between aging and osteoarthritis (OA), but the specific mechanisms are still unclear. This study aims to explore the potential connections and molecular mechanisms between OA and aging through multi-omics and genetics methods. Integrating single-cell RNA sequencing (scRNA-seq), bulk RNA-seq data, Mendelian randomization (MR), colocalization analysis, and cell function analysis, this study explores the correlation between OA and aging. Furthermore, it investigates the potential causal relationship between key marker genes and OA. Integrating and analyzing scRNA-seq data from OA, aging, and control groups revealed a significant increase in the proportion of the classical monocyte core subgroup. Differential expression analysis yielded 77 marker genes, and further MR analysis identified four key marker genes (DUSP6, CSTA, CD300E, and GPX1) as causally related to OA, which was confirmed in an independent validation cohort. Reverse MR and Steiger filtering indicated no evidence of reverse causality. DUSP6- and CSTA-classical monocytes may interact with other cell subgroups through the MIF-(CD74 + CD44) signaling pathway. This study revealed the heterogeneity of monocyte subgroups in OA and aging patients, identifying key marker genes with a causal relationship to OA through an integrated multi-omics approach, providing potential molecular targets for the diagnosis and treatment of OA from an aging perspective.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of an Adjuvant-Free Single Polypeptide-Based Tuberculosis Subunit Vaccine that Elicits In Vivo Immunogenicity in Rats.","authors":"Supriya Phogat, Jyoti Yadav, Darshna Chaudhary, Ranjana Jaiwal, Pawan K Jaiwal","doi":"10.1007/s12033-025-01431-7","DOIUrl":"https://doi.org/10.1007/s12033-025-01431-7","url":null,"abstract":"<p><p>A novel tuberculosis subunit vaccine specific for Mycobacterium tuberculosis dual antigens, culture filtrate protein-10 (CFP-10) and antigen 85B (Ag85B) conjugated with cholera toxin non-toxic B subunit (CTB), was expressed as a single polypeptide in high amounts and cost-effectively in Escherichia coli. The recovery and purification conditions for the recombinant fusion protein were established. This simple peptide vaccine required no exogenous adjuvant as it contained CTB, a potent immune modulator. The vaccine's physiochemical, structural, and immunological properties were determined using the in-silico tools. It was highly antigenic, non-allergenic, and non-toxic. Its BlastP search with human proteomes excluded the chances of autoimmune reactions. The tertiary structure model (3D) was validated by Ramachandran plot assessment. The 3D structure docking with Toll-like receptors, TLR-1, 2, 4, and 6, showed that the binding affinity between the vaccine peptide and TLRs was high, and their complex was stable, indicating a strong immune response. The in-silico immune simulation revealed the vaccine-induced both innate and adaptive immune responses. In-vivo validation of the immunogenicity of CTB.CFP10.Ag85B in Wistar rats revealed higher activation of IgG immune response compared to either antigen protein. Similar results were also obtained using the C-ImmSim simulation online server. A comparison of immunogenicity of CTB.CFP10.Ag85B with the only available TB vaccine, Bacillus Calmette-Guérin (BCG) or as a booster after vaccination of Wistar rats with BCG, indicated that the IgG levels were the highest in rats vaccinated with BCG, followed by a booster dose of CTB.CFP10.Ag85B fusion protein. The fusion protein would be a safe potential vaccine booster candidate in BCG-primed individuals against TB.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution and Impact of Imine Reductases (IREDs) Research: A Knowledge Mapping Approach.","authors":"Qilong Yao, Yujun Hu, Ziwei Liu, Jun-Zhong Liu, Qing-Cai Jiao","doi":"10.1007/s12033-025-01421-9","DOIUrl":"https://doi.org/10.1007/s12033-025-01421-9","url":null,"abstract":"<p><p>Nitrogen-containing compounds, particularly those with chiral amine structures, play a crucial role in the development of organic active pharmaceutical ingredients. Imine reductases (IREDs), NAD(P)H-dependent enzymes that catalyze the reduction of cyclic imines and the reductive amination of prochiral ketones, offer significant industrial potential for the synthesis of chiral amines. However, despite the growing body of research, a comprehensive and unbiased assessment of IRED research remains lacking. This study aims to explore the research landscape and evolution of IREDs using bibliometric and knowledge mapping methods. A total of 239 research articles and reviews on IREDs, published between 2010 and 2024, were retrieved from the Web of Science Core Collection and analyzed using tools such as CiteSpace, VOSviewer, Pajek, and Scimago Graphica. Results showed a consistent increase in both publications and citations, with a sharp rise since 2014. Collaboration network analysis revealed that the United Kingdom leads the field in terms of publications and influential institutions, while ChemCatChem was identified as the journal with the highest number of articles. Nicholas J. Turner emerged as a key researcher, having published the most papers and achieving the second-highest citation frequency. Research trends and keyword analysis highlighted areas of focus such as IRED crystal structure resolution, protein engineering modifications, and expanded industrial applications, including multi-enzyme cascade reactions. Ongoing advancements in synthetic biology, protein modifications, and enzyme engineering are expected to drive further studies on highly active IREDs for asymmetric synthesis of pharmaceutical compounds, positioning this research at the forefront of the field. By employing bibliometric analysis, this study provides the first visual representation of IRED research, offering valuable insights into current trends and emerging topics that will aid scholars in identifying key research areas and potential collaborators.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Anatomical Nano Interbody Fusion Device for Elderly Patients Undergoing Anterior Discectomy and Interbody Fusion.","authors":"Shuang Tian, Pengliang Xu","doi":"10.1007/s12033-025-01427-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01427-3","url":null,"abstract":"<p><p>To evaluate the perioperative outcomes of a novel anatomical nano-cage in elderly patients undergoing anterior discectomy and interbody fusion, with special attention to its outcomes on clinical symptoms, interbody fusion stability, and surgery-related complications.</p><p><strong>Methods: </strong>twenty-four cases of elderly surgical patients were divided into control group 1 (Cntrl 1), control group 2 (Cntrl 2), control group 3 (Cntrl 3), and the Experimental group (Exptl), 6 cases in each group. The polyetheretherketone interbody fusion cage was used in Cntrl 1 group, the titanium alloy interbody fusion cage was used in Cntrl 2 group, the spine minimally invasive interbody fusion cage was used in Cntrl 3 group, and the nano-hydroxyapatite/polyamide 66 (nHA/PA66) interbody fusion cage was used in Exptl group. All patients received anterior discectomy and interbody fusion, and received perioperative nursing intervention. The management of postoperative complications and patient observation indexes were recorded in detail.</p><p><strong>Results: </strong>The fusion of the fusion cage was better in the Exptl group than in the Cntrl group after surgery. At 2 weeks, 3 months, and 6 months after operation, the intervertebral space height of Exptl group was higher than that of Cntrl group (P < 0.05). Visual analogue scale (VAS) was lower in Exptl group than in Cntrl group (P < 0.05). At 2 weeks and 3 months after surgery, Japanese Orthopaedic Association (JOA) scores were higher in Exptl group than in Cntrl group (P < 0.05). Complications occurred more frequently in the Exptl group than in the Cntrl group.</p><p><strong>Conclusion: </strong>the novel anatomical nano-interbody fusion markedly improved the stability, the effective implementation of perioperative care reduced the incidence of complications, and the postoperative follow-up suggested that the successful application of interbody fusion cage had a positive impact on the overall rehabilitation of patients.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of C/EBP Homologous Protein (CHOP) and Nupr1 Interaction in Endoplasmic Reticulum Stress-Induced Apoptosis of Lens Epithelial Cells.","authors":"Jinghua Li, Junyi Liu, Yongying Tang, Hong Zhang, Yuanping Zhang, Xu Zha, Xueying Zhao","doi":"10.1007/s12033-024-01148-z","DOIUrl":"10.1007/s12033-024-01148-z","url":null,"abstract":"<p><p>Our study mainly analyzed the mechanism of C/EBP homologous protein (CHOP) and its interacting protein Nupr1 on endoplasmic reticulum stress (ERS) induced lens epithelial cells (LEC) apoptosis. Cell proliferation was detected by CCK-8. Apoptosis was detected by flow cytometry and TUNEL. Nupr1 expression was detected by RT-qPCR. The expressions of CHOP, Nupr1, apoptosis-related protein, and ERS-related protein were detected by Western blot. DCFH-DA probe was used to detect cell ROS. The SOD, GSH-PX, and MDA contents were detected by the kit. Co-IP was used to detect the interaction between CHOP and Nupr1. The morphology of the lens was detected by HE staining. The result shows that Tunicamycin (TU) can induce endoplasmic reticulum stress and apoptosis in LEC in a concentration-dependent manner. TU induction leads to the occurrence of CHOP nuclear translocation. Overexpression of CHOP can further enhance the inhibitory effect of TU on LEC proliferation and the promotion of apoptosis, while knockdown of CHOP has the opposite effect. CHOP and Nupr1 are interacting proteins, and knockdown of Nupr1 or addition of Nupr1 inhibitor ZZW-115 can reverse the effects of TU and overexpression of CHOP, respectively. It has been observed in animal experiments that treatment with oe-CHOP can further aggravate the pathological lesions of the rat lens, while ZZW-115 can reverse the effect of oe-CHOP to a certain extent and improve the lesions of the rat lens. Overall, CHOP interacts with Nupr1 to regulate apoptosis caused by ERS and mediate cataract progression in rats, and this study provides a new potential therapeutic target for the treatment of cataract.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1628-1640"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}