Molecular Biotechnology最新文献_第10页

Bioinformatics Analysis Reveals HIST1H2BH as a Novel Diagnostic Biomarker for Atrial Fibrillation-Related Cardiogenic Thromboembolic Stroke. 生物信息学分析揭示 HIST1H2BH 是心房颤动相关心源性血栓栓塞性中风的新型诊断生物标记物

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-06-02 DOI: 10.1007/s12033-024-01187-6

Wenbing Jiang, Lelin Jiang, Xiaoli Zhao, Yiying Liu, Huanghui Sun, Xinlang Zhou, Yin Liu, Shu'se Huang

{"title":"Bioinformatics Analysis Reveals HIST1H2BH as a Novel Diagnostic Biomarker for Atrial Fibrillation-Related Cardiogenic Thromboembolic Stroke.","authors":"Wenbing Jiang, Lelin Jiang, Xiaoli Zhao, Yiying Liu, Huanghui Sun, Xinlang Zhou, Yin Liu, Shu'se Huang","doi":"10.1007/s12033-024-01187-6","DOIUrl":"10.1007/s12033-024-01187-6","url":null,"abstract":"Atrial fibrillation (AF) is a significant precursor to cerebral embolism. Our study sought to unearth new diagnostic biomarkers for atrial fibrillation-related cerebral embolism (AF-CE) by meticulously examining multiple GEO datasets and meta-analysis. The gene expression omnibus (GEO) database provided RNA sequencing data associated with AF and stroke. We began by pinpointing genes with varied expressions in AF-CE patient blood samples. A meta-analysis was subsequently undertaken using several RNA sequencing datasets to verify these genes. LASSO regression discerned key genes for AF-CE, with their diagnostic prowess verified through ROC curve examination. Active signaling pathways within stroke patients were discerned via GO and KEGG enrichment, with PPI interactions detailing gene interplay. Differential gene analysis revealed an upregulation of sixteen genes and a downregulation of four in stroke patient blood samples. Eight genes showcased varied expression in the meta-analysis. LASSO regression zeroed in on five of these, culminating in HIST1H2BH's identification as a characteristic gene. HIST1H2BH's prowess in predicting AF-CE was confirmed through ROC. Integrin signaling, platelet activation, ECM interactions, and the PI3K-Akt pathway were found active in stroke victims. HIST1H2BH's interaction with the notably upregulated ITGA2B was spotlighted by PPI. Additionally, HIST1H2BH exhibited links with NK cells and eosinophils. HIST1H2BH emerges as an insightful diagnostic beacon for AF-CE. Its presence, post AF, potentially modulates pathways, accentuating platelet activation and consequent thrombus generation, leading to cerebral embolism.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2111-2126"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCDHGA10 as a Potential Biomarker of Lung Squamous Cell Carcinoma Based on Bioinformatics and Experimental Verification. 基于生物信息学和实验验证的肺鳞癌潜在生物标记物 PCDHGA10

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-10 DOI: 10.1007/s12033-024-01178-7

Shuming Song, Ruijiao Lu, Yuanya Chen, Yangchun Feng

{"title":"PCDHGA10 as a Potential Biomarker of Lung Squamous Cell Carcinoma Based on Bioinformatics and Experimental Verification.","authors":"Shuming Song, Ruijiao Lu, Yuanya Chen, Yangchun Feng","doi":"10.1007/s12033-024-01178-7","DOIUrl":"10.1007/s12033-024-01178-7","url":null,"abstract":"Procalcitonin gamma subfamily A, 10 (PCDHGA10) is a member of the procalcitonin gamma gene cluster, which is associated with neuronal synapse development. However, there are lack of studies on the role and potential prognostic value of PCDHGA10 in lung squamous cell carcinoma (LUSC). We analyzed the RNAseq data of PCDHGA10 to compare their expression differences. Then survival analysis, tumor microenvironment (TME) analysis, and mutation analysis were carried out. Additionally, we performed gene ontology (GO) and kyoto gene encyclopedia (KEGG) enrichment analyses to explore potential signal pathways. PCDHGA10 protein expression was evaluated using immunohistochemistry (IHC) on tissue microarrays (HLugS180Su02). By microarray analysis and database analysis, we found that PCDHGA10 was significantly highly expressed in LUSC. Sufferers with elevated PCDHGA10 levels exhibited a worse prognosis, according to the survival analysis. The PCDHGA10 mutation was also linked to LUSC patient prognosis. Besides, PCDHGA10 was closely related to tumor immune cell infiltration and immune checkpoints. In conclusion, PCDHGA10 is expected to become a new molecular marker for LUSC.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2002-2022"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of OsCCR1, OsCOMT5, OsCAD2 and OsCCoAOMT1 Genes Enhances Lignin Accumulation and Confers Tolerance Against Rhizoctonia solani in Rice (Oryza sativa). OsCCR1、osccomt5、OsCAD2和OsCCoAOMT1基因的过表达增强了水稻木质素积累并增强了对茄枯核菌的抗性。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 DOI: 10.1007/s12033-025-01436-2

Gurdeep Kaur, Gurbir Kaur Sidhu, Anjulata Singh, Swatismita Dhar-Ray, Sangram Keshari Lenka, Pallavolu Maheshwara Reddy

{"title":"Overexpression of OsCCR1, OsCOMT5, OsCAD2 and OsCCoAOMT1 Genes Enhances Lignin Accumulation and Confers Tolerance Against Rhizoctonia solani in Rice (Oryza sativa).","authors":"Gurdeep Kaur, Gurbir Kaur Sidhu, Anjulata Singh, Swatismita Dhar-Ray, Sangram Keshari Lenka, Pallavolu Maheshwara Reddy","doi":"10.1007/s12033-025-01436-2","DOIUrl":"https://doi.org/10.1007/s12033-025-01436-2","url":null,"abstract":"The necrotrophic fungus, Rhizoctonia solani is the major cause of sheath blight, a disease that leads to a significant reduction in rice yield, posing a serious threat to food security. Traditional breeding approaches have struggled to develop effective resistance, highlighting the importance of transgenic technology as a promising solution. This study explored the relationship between enhanced lignin production and the overexpressing key lignin biosynthesis genes (OsCCR1, OsCOMT5, OsCAD2, and OsCCoAOMT1), demonstrating that increased lignin accumulation strengthens defense mechanisms against R. solani by preventing its penetration of the cell wall. Thioacidolysis analysis revealed higher lignin levels in the leaf sheath tissues of OX-OsCCR1 transgenic rice plants, which effectively blocked fungal hyphae invasion, as confirmed by confocal and scanning electron microscopy. Similarly, the cell walls of OX-OsCOMT5 transgenic lines accumulated significantly higher levels of cell wall-bound phenolics, which inhibited R. solani ingress and infection peg formation. Notably, OX-4C transgenic rice plants, overexpressing all four lignin biosynthesis genes, exhibited elevated levels of lignin in the leaf sheath during early infection, serving as a robust first line of defense. These findings underscore the critical role of cell wall restructuring, particularly through increased lignin deposition, in combating sheath blight infection and enhancing crop resilience. Engineering the lignin biosynthesis pathway provides a promising approach for developing broad-spectrum resistance to fungal pathogens in other economically important crops, paving the way for sustainable agriculture and food security.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EPB41L3 Inhibits the Progression of Cervical Cancer Via the ERK/p38 MAPK Signaling Pathway. EPB41L3 通过 ERK/p38 MAPK 信号通路抑制宫颈癌的进展

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-06-21 DOI: 10.1007/s12033-024-01172-z

Gulixian Tuerxun, Wenyun Li, Guligeina Abudurexiti, Qian Zhuo, Awahan Tuerdi, Guzalinuer Abulizi

{"title":"EPB41L3 Inhibits the Progression of Cervical Cancer Via the ERK/p38 MAPK Signaling Pathway.","authors":"Gulixian Tuerxun, Wenyun Li, Guligeina Abudurexiti, Qian Zhuo, Awahan Tuerdi, Guzalinuer Abulizi","doi":"10.1007/s12033-024-01172-z","DOIUrl":"10.1007/s12033-024-01172-z","url":null,"abstract":"This study was aimed to uncover the character and potential regulatory mechanism of EPB41L3 in cervical cancer (CC). CC cells were injected into BALB/c nude mice (female) to construct a xenograft tumor model. Real-time quantitative polymerase chain reaction (qRT-PCR) and western blot were performed to evaluate the expression of EPB41L3, ERK/p38 MAPK signal markers in CC tissues and cells. Cell counting kit-8 (CCK-8) and Transwell was applied to analyze the viability, invasion, and migration of CC cell lines. EPB41L3 was substantially decreased both in CC tissues and cells. Cell viability, invasion, and migration of CC cells were reduced by overexpressing EPB41L3. Bioinformatics analysis prerdicted that EPB41L3 was strongly related to the ERK/p38 MAPK pathway. Compared with Ad-nc mice, the volume and weight of tumors and ERK/p38 MAPK signal markers were down-regulated in Ad-EPB41L3 mice. After knocking down EPB41L3 with EPB41L3 siRNA (siEPB41L3), the ERK/p38 MAPK pathway was activated. Moreover, SB203580 treatment reversed the effect of EPB41L3 silencing on the improvement in viability, migration, and invasion of CC cells. EPB41L3 suppresses the progression of CC via activating the ERK/p38 MAPK pathway. EPB41L3 may serve as an effective therapeutic target for CC.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1958-1967"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brevilin A Inhibits Prostate Cancer Progression by Decreasing PAX5-Activated SOX4. Brevilin A 通过减少 PAX5 激活的 SOX4 抑制前列腺癌的发展

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-14 DOI: 10.1007/s12033-024-01183-w

Xinxiang Que, Jianqun Fan, Desheng Chen, Zhen Nie, Peng Chen

{"title":"Brevilin A Inhibits Prostate Cancer Progression by Decreasing PAX5-Activated SOX4.","authors":"Xinxiang Que, Jianqun Fan, Desheng Chen, Zhen Nie, Peng Chen","doi":"10.1007/s12033-024-01183-w","DOIUrl":"10.1007/s12033-024-01183-w","url":null,"abstract":"Brevilin A possesses inhibitory effects on the development of prostate cancer (PCa); however, the underlying mechanism remains unclear. The present work aims to analyze how Brevilin A regulates PCa cell malignancy. RNA expression of paired box 5 (PAX5) and SRY-box transcription factor 4 (SOX4) was analyzed by quantitative real-time polymerase chain reaction. Protein expression of PAX5, SOX4, and nuclear proliferation marker (Ki67) was detected by western blotting or immunohistochemistry assay. The viability, proliferation, apoptosis, and migratory and invasive abilities of PCa cells were investigated by cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU), flow cytometry, and transwell assays, respectively. The association between PAX5 and SOX4 was identified by dual-luciferase reporter assay and chromatin immunoprecipitation assay. Xenograft mouse model assay was used to reveal the effect of Brevilin A on tumor tumorigenesis in vivo. PAX5 and SOX4 expression were upregulated in PCa tissues and cells relative to normal prostate tissues and human prostate epithelial cells. Brevilin A treatment inhibited PAX5 protein expression in PCa cells. Additionally, Brevilin A inhibited proliferation, migration and invasion and induced apoptosis of PCa cells, whereas these effects were attenuated after PAX5 overexpression. SOX4 was transcriptionally activated by PAX5, and its introduction partially relieved the inhibitory effects of PAX5 knockdown on PCa cell malignancy. Moreover, Brevilin A delayed tumor formation in vivo. Brevilin A inhibited PCa progression by regulating SOX4 expression in a PAX5-dependent manner, providing a promising anti-tumor drug for PCa.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2060-2071"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical Screening and Computational Insights into VP26 Inhibitors for Mitigating White Spot Syndrome Virus in Shrimp Aquaculture. 对虾养殖中减轻白斑综合征病毒VP26抑制剂的植物化学筛选和计算分析

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-29 DOI: 10.1007/s12033-025-01442-4

S J Md Ashiq, A C Snekha, T Muthu Kumar, U Dhivya Dharshini, Zainulabidin Ashiru Aliyu

{"title":"Phytochemical Screening and Computational Insights into VP26 Inhibitors for Mitigating White Spot Syndrome Virus in Shrimp Aquaculture.","authors":"S J Md Ashiq, A C Snekha, T Muthu Kumar, U Dhivya Dharshini, Zainulabidin Ashiru Aliyu","doi":"10.1007/s12033-025-01442-4","DOIUrl":"https://doi.org/10.1007/s12033-025-01442-4","url":null,"abstract":"White spot syndrome disease (WSSD) is a contagious disease caused by white spot syndrome virus (WSSV) in shrimp aquaculture. Given its high degree of contagiousness, controlling its rapid spread has proven to be challenging, causing significant economic loss to farmers. To prevent these losses, farmers often resort to the use of large doses of general aquaculture antibiotics. However, prolonged exposure to such antibiotics can lead to adverse effects for both the shrimp and the humans consuming them. Additionally, this practice contributes to the global issue of antimicrobial resistance. Currently, there are no vaccines or antibiotics that specifically target this virus. Therefore, exploring potential compounds through virtual screening offers a promising avenue for finding effective solutions. A total of 1683 phytochemical metabolites from 40 medicinal plants were screened against the target VP26, which plays a pivotal role in virus maturation. Initial screening was performed via ADMET and molecular docking analysis. Furthermore, we evaluated the binding affinity via machine learning-based scoring schemes. Importantly, the compounds displayed applicable toxicity properties during testing with ECOSAR. The binding ability of the compounds was validated with 150 ns of MD simulation. Overall, isocolumbin and urolithin A 3-O-glucuronide had significant effects on the outcomes of all the analyses. Therefore, we believe that this compound could be an alternative therapeutic option to the WSS virus in shrimp aquaculture.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pFastBac4x: A Novel Vector for the Simultaneous Expression of Four Proteins-Expression and Purification of a G-Protein Complex as a Case Study. pFastBac4x：一种同时表达四种蛋白的新载体——g蛋白复合物的表达和纯化的案例研究

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-28 DOI: 10.1007/s12033-025-01434-4

Ioanna Ramou, Steven Janvier, Pieter Claes, Christel Menet, Jan Steyaert, Els Pardon

引用次数: 0

LINC00926, Regulated by TCF12, Modulates the Ubiquitination of GPX4 to Regulate Ferroptosis by Interacting with STUB1 in HUVECs. TCF12调控的LINC00926通过与STUB1相互作用调控huves中GPX4的泛素化

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-28 DOI: 10.1007/s12033-025-01441-5

Yong Jiang, Han-Zhu Zhou, Jun-Xuan Zhang, Kai-Qi Li, Jia-He Wang

{"title":"LINC00926, Regulated by TCF12, Modulates the Ubiquitination of GPX4 to Regulate Ferroptosis by Interacting with STUB1 in HUVECs.","authors":"Yong Jiang, Han-Zhu Zhou, Jun-Xuan Zhang, Kai-Qi Li, Jia-He Wang","doi":"10.1007/s12033-025-01441-5","DOIUrl":"https://doi.org/10.1007/s12033-025-01441-5","url":null,"abstract":"LINC00926 has been identified as an upregulated lncRNA in patients with coronary heart disease (CHD) through high-throughput sequencing. This study aimed to explore the biological role of LINC00926 in vascular endothelial cell ferroptosis and its underlying mechanisms. For in vitro experiments, HUVECs were exposed to hypoxic conditions. Our results showed an upregulation of LINC00926 expression, a decrease in GPX4 and GSH levels, and an increase in MDA and ROS levels in hypoxia-treated HUVECs. Furthermore, the ferroptosis inhibitor (ferrostatin-1) reversed the decrease in cell viability induced by hypoxia, suggesting that hypoxia treatment triggered GPX4-mediated ferroptosis in HUVECs. These variations were further exacerbated when LINC00926 was overexpressed, but were partially mitigated when LINC00926 was silenced. Notably, LINC00926 had no effect on GPX4 mRNA levels. Our data proved that LINC00926 modulated the ubiquitination and degradation of GPX4 via STUB1, thereby promoting hypoxia-induced HUVEC ferroptosis. Additionally, ChIP and luciferase reporter gene assays confirmed that TCF12 protein enhanced the transcriptional activity of LINC00926 promoter, hinting TCF12 is an upstream regulator of LINC00926. Besides, LINC00926 also enhanced the stability of TCF12 mRNA to promote TCF12 expression. Moreover, TCF12 acted as a regulator of ferroptosis in hypoxia-induced HUVECs. Finally, rescue experiments determined the role of the TCF12/LINC00926/GPX4 axis in ferroptosis of HUVECs upon hypoxic stimulation. In conclusion, this study demonstrated that the TCF12/LINC00926/GPX4 axis plays a regulatory role in hypoxia-induced ferroptosis of HUVECs, offering a promising target for the treatment of CHD.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Mesenchymal Stem Cells in Niemann-Pick Disease. 间充质干细胞治疗尼曼-匹克病的潜力。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-25 DOI: 10.1007/s12033-025-01435-3

Keli Xu, Minlin Yang, Lihong Guan, Ciqing Yang, Liang Qiao, Yonghai Li, Juntang Lin, Xiaoying Li

{"title":"Therapeutic Potential of Mesenchymal Stem Cells in Niemann-Pick Disease.","authors":"Keli Xu, Minlin Yang, Lihong Guan, Ciqing Yang, Liang Qiao, Yonghai Li, Juntang Lin, Xiaoying Li","doi":"10.1007/s12033-025-01435-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01435-3","url":null,"abstract":"Niemann-Pick disease (NPD) is a rare autosomal recessive neurodegenerative disease characterized by hepatosplenomegaly, neuropathy, and a significantly shortened lifespan. Lipid metabolism disorder is the main pathological feature of NPD. Currently, the exact pathogenesis of NPD remains unclear, and drug therapy is largely palliative, focusing on symptom management, but it has side effects. Mesenchymal stem cells (MSCs) possess several advantageous properties, including their differentiation potential, wide availability, low immunogenicity, and the ability to secrete regulatory factors, which have led to their extensive application in basic research targeting neurodegenerative diseases. Studies have demonstrated that transplantation of MSCs from different sources into animal models of NPD can delay the loss of Purkinje cells in the cerebellum, reduce lipid deposition, improve motor coordination, slow the rate of weight loss, and extend lifespan. This review explores the therapeutic potential of MSCs in the treatment of NPD, highlighting their emerging role in addressing this challenging condition.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dingkun Pill-Mediated TGF-β1/Smad2 Pathway Effects on Granulosa Cell Proliferation and Apoptosis. 定坤丸介导的TGF-β1/Smad2通路对颗粒细胞增殖和凋亡的影响

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-25 DOI: 10.1007/s12033-025-01439-z

Lan Chen, Xiaojing Yan, Yuanpei Lian, Dijun Wang, Jiali Cai, Chunyan Yin, Ji Cao

{"title":"Dingkun Pill-Mediated TGF-β1/Smad2 Pathway Effects on Granulosa Cell Proliferation and Apoptosis.","authors":"Lan Chen, Xiaojing Yan, Yuanpei Lian, Dijun Wang, Jiali Cai, Chunyan Yin, Ji Cao","doi":"10.1007/s12033-025-01439-z","DOIUrl":"https://doi.org/10.1007/s12033-025-01439-z","url":null,"abstract":"To investigate influences of Dingkun Pill on granulosa cell proliferation and apoptosis (P&A) in polycystic ovary syndrome (PCOS) mice and its association with the TGF-β1/Smad2 signaling pathway (SPW) activity. Forty-eight female SD mice were rolled into experimental group (EG, Dingkun Pill), control group (CG, metformin), model group (MG, distilled water), and normal group (NG, distilled water), with 12 mice per group. The Beloosesky method was employed to establish the PCOS mouse model. Serum hormone (follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T)), expression of vascular endothelial growth factor (VEGF) in rat ovarian tissue and expression of HOXA10 in endometrial tissue, cell P&A, and expression level (EL) of TGF-β1 and Smad2 were compared among groups. Compared to the MG, the EG and CG exhibited significantly reduced levels of FSH, LH, and testosterone, while estradiol levels were significantly elevated. Furthermore, granulosa cell proliferation was notably enhanced, and the apoptosis rate was significantly decreased (P < 0.05). In addition, the expression of VEGF in ovarian tissue, as well as the expression of TGF-β1 and Smad2, was significantly lower in EG and CG compared to MG, while the expression of the HOXA10 gene in the endometrium was significantly increased (P < 0.05). Compared to CG, EG showed higher ovarian VEGF expression and lower HOXA10 gene expression (P < 0.05).Dingkun Pill notably improves serum hormone in a PCOS mouse model, inhibits granulosa cell apoptosis, and enhances granulosa cell proliferation activity. This effect is likely mediated through the inhibition of the TGF-β1/Smad2 SPW, suggesting a protective role of Dingkun Pill in PCOS.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0