Molecular Biotechnology最新文献

{"title":"Subtractive Proteomics-Based Prioritization of Drug Targets in Enterococcus faecalis V583 and Structure-Based Identification of Plant-Derived Inhibitors Targeting Alanine Racemase: A High Priority Target.","authors":"Muthusamy Sureshan, Nithyashree Sriram, Arunachalam Jothi","doi":"10.1007/s12033-026-01558-1","DOIUrl":"10.1007/s12033-026-01558-1","url":null,"abstract":"<p><p>Enterococcus faecalis, a Gram-positive facultative anaerobe, is a typical inhabitant of the human gut microbiota but becomes an opportunistic pathogen when translocated, leading to serious infections such as meningitis and bloodstream infections (BSIs). According to the World Health Organization (WHO) and recent studies, E. faecalis is responsible for a significant number of BSIs and is listed as a high-priority pathogen for antimicrobial research due to its high antibiotic resistance rates. Despite the availability of antibiotics like ampicillin, vancomycin, and amoxicillin, the emergence of resistant strains necessitates the discovery of novel drug targets. This study employed a subtractive proteomics approach to identify potential therapeutic targets in the genome of E. faecalis V583. A total of 161 essential, non-homologous proteins unique to the pathogen were identified as candidate drug targets. Among them, nine were key enzymes involved in vital metabolic pathways such as flagellar assembly, peptidoglycan biosynthesis, and amino acid metabolism. Notably, Alanine racemase (EfAlaR), essential for cell wall synthesis, was highlighted as a unique, non-homologous target. Structure-based virtual screening identified five phytochemicals, Woodfordin A (- 17.838 kcal/mol), Oenothein B (- 14.826 kcal/mol), Euphorbin H (- 14.767 kcal/mol), Pentagalloylglucose (- 14.557 kcal/mol), and Euphorbin G (- 14.526 kcal/mol) with strong binding affinities toward EfAlaR, highlighting them as promising lead compounds for the development of anti-E. faecalis therapeutics.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3229-3258"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of SRCA for Rapid Detection of Pseudomonas aeruginosa in Bottled Drinking Water.","authors":"Lianbao Zheng, Jiahong Zhu, Xiaohong Zhang, Yunbin Yang, Hongxia Yang, Tong Hu, Weihong Zhong","doi":"10.1007/s12033-026-01557-2","DOIUrl":"10.1007/s12033-026-01557-2","url":null,"abstract":"<p><p>Pseudomonas aeruginosa is a significant waterborne pathogen that poses substantial public health risks, particularly in bottled drinking water. Traditional methods for detecting P. aeruginosa are often time-consuming, expensive, and require complex equipment. This study developed a novel Saltatory Rolling Circle Amplification (SRCA) method for the rapid, sensitive, and specific detection of P. aeruginosa in bottled drinking water. The technique utilizes a simple water bath for isothermal DNA amplification and incorporates SYBR Green I for visual colorimetric detection, eliminating the need for complex post-amplification processes such as gel electrophoresis. The SRCA assay demonstrated high sensitivity with a detection limit of 9.1 × 10¹ CFU/mL and exhibited excellent specificity, as amplification products were only detected in samples containing P. aeruginosa DNA. A clear colorimetric response allowed straightforward interpretation, with positive samples exhibiting distinct green fluorescence. Evaluation using 200 artificially contaminated samples revealed that the SRCA method achieved 100.00% sensitivity, 98.90% specificity, and 99.00% accuracy compared to conventional microbiological culture. This SRCA-based assay therefore represents a promising tool for rapid, cost-effective, and on-site detection of P. aeruginosa in drinking water, with potential applications for enhancing public health safety in both industrial and field environments.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3219-3228"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Review on CRISPR-Based Screening and Its Applications.","authors":"Ali Saber Sichani, Mahmoud Hassani, Fatemeh Gila, Negin Shafieipour, Reza Dabbaghipour, Zahra Heidari, Mohsen Sisakht, Mohammad Hassani, Jafar Fallahi","doi":"10.1007/s12033-026-01562-5","DOIUrl":"10.1007/s12033-026-01562-5","url":null,"abstract":"<p><p>CRISPR-based tools have quickly moved from specialist techniques to routine instruments in biology and medicine, and they are now central to large-scale loss-of-function and perturbation screens. In this review, we focus on how pooled CRISPR screens are used to interrogate gene function in living cells, most often through cell fitness or simple selectable markers, and contrast this with arrayed formats that trade throughput for richer molecular readouts, such as transcriptome-wide changes. We bring together current strategies for library design, delivery, and selection and show how different Cas nucleases, including Cas9, Cas12, and Cas13, broaden the range of genome and transcriptome perturbations that can be assayed. We then discuss recent applications in drug response, viral infection, and cancer biology and consider how improvements in high-content technologies, data analysis, and emerging diagnostic uses are likely to shape the next generation of CRISPR-based screening studies.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3051-3067"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147723370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection Strategies for Begomoviruses: A Focus Review of Methods.","authors":"Nguyen Hoai Nguyen, Quynh Xuan Thi Luong, Thuy Thi Bich Vo","doi":"10.1007/s12033-026-01555-4","DOIUrl":"10.1007/s12033-026-01555-4","url":null,"abstract":"<p><p>Begomovirus, the largest genus within the family Geminiviridae, poses a significant threat to global agriculture, resulting in substantial economic losses across diverse cropping systems. The increasing incidence and geographic spread of begomoviruses are driven by both the emergence of novel viral strains and the adaptation of existing ones, complicating efforts to manage the disease effectively. Traditional techniques, such as PCR and ELISA, have long been the primary methods used for detecting begomoviruses. However, recent advancements have introduced modern detection techniques designed to improve speed and accuracy. Additionally, some techniques can also be applied in the field with commercial products, enhancing the practical efficiency of begomoviruses detection. This review summarizes the most used methods for begomoviruses detection and provides a comparative assessment of the strengths and limitations of each technique. Based on this, it will assist researchers in gaining a comprehensive overview of detection methods and their applications in studying this plant virus genus. Consequently, researchers can select the optimal detection method for their studies without expending excessive time and resources.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2972-2988"},"PeriodicalIF":3.2,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.","authors":"Dilsat Nigar Colak, Ali Osman Belduz, Halil Ibrahim Guler, Esma Ceylan Yerebakan, Ayse Cebeci","doi":"10.1007/s12033-026-01591-0","DOIUrl":"https://doi.org/10.1007/s12033-026-01591-0","url":null,"abstract":"<p><p>DNA ligases are essential enzymes in all living organisms and viruses. They play critical roles in genome maintenance and recombination by sealing single-strand nicks and repairing damaged DNA. Due to these features, DNA ligases are widely used in gene cloning applications and in vitro processing of DNA. In this study, the DNA ligase-encoding gene from Trabzonvirus AP-T65 was cloned and sequenced. AP-T65 DNA ligase (AhyAP-T65Lig) shared 51.81% sequence similarity with the DNA ligase of Aeromonas phage BUCT695, and 53.35% with that of Salmonella phage PKM.Hi.22.6. AhyAP-T65Lig was cloned into pET-28a(+) expression vector, overexpressed and purified. The ligation efficiency of AhyAP-T65Lig (41.1 kDa) was evaluated using ligation-mediated PCR and RT-PCR. AhyAP-T65Lig was active between 4 and 37 °C, with an optimum temperature of 4 °C and an optimum pH of 8.0. Maximum activity was observed in the presence of 1 mM ATP, 10 mM DTT, 10 mM MgCl_2, and 1 mM NaCl. Sticky-ended, linearized pGEM-T Easy and pET-28a(+) vectors were circularized by AhyAP-T65Lig. The enzyme retained its activity for an extended period at both 4 and - 20 °C. The low-temperature optimum, high in vitro yield, and long shelf-life of AhyAP-T65Lig make it a promising enzyme for biotechnological applications.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148345874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.","authors":"Zi-Xu Wang, Qiu-Jun Mo, Jun-Wei Xu","doi":"10.1007/s12033-026-01589-8","DOIUrl":"https://doi.org/10.1007/s12033-026-01589-8","url":null,"abstract":"<p><p>The ATP-citrate lyase gene (acl) was cloned and overexpressed in Ganoderma lingzhi. Overexpression of acl increased the accumulation of ganoderic acids (GAs) in G. lingzhi in both liquid shake culture and solid-state fermentation (SSF) conditions, with higher production observed in the SSF. In the SSF, the maximum contents of individual GAs (GA-Mk, GA-T, GA-S, and GA-Me) and total GAs in the acl-overexpressing G. lingzhi (ACL strain) reached 107.65 ± 3.19, 301.82 ± 27.38, 152.65 ± 15.28, and 259.77 ± 23.42 μg/100 mg dry weight (DW) and 9.47 ± 0.61 mg/100 mg DW, respectively, representing 1.42-, 1.74-, 2.01-, 1.85-, and 1.72-fold higher than those of the wild-type (WT) strain. The ACL strain also showed elevated ACL enzyme activity (145.23 ± 8.81 U/g) and acetyl-CoA content (74.85 ± 3.23 μg/g), which were 2.08 and 2.82 times greater than those of the WT strain, respectively. Additionally, the maximum levels of the key intermediates squalene and lanosterol were improved by 1.77- and 1.54-fold, respectively, and transcript levels of the genes encoding 3-hydroxy-3-methylglutaryl coenzyme A enzyme (hmgr), squalene synthase (sqs), and lanosterol synthase (ls) were upregulated by 3.46-, 7.21-, and 5.85-fold, respectively, compared with those in the WT strain. Collectively, these results demonstrate that overexpression of the acl is an effective strategy for enhancing GA biosynthesis in G. lingzhi.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148331326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.","authors":"Mahavir Joshi, Pari Panwar, Smile Sharma, Bharat Sagar, Sukhminderjit Kaur, Manikant Tripathi","doi":"10.1007/s12033-026-01585-y","DOIUrl":"https://doi.org/10.1007/s12033-026-01585-y","url":null,"abstract":"<p><p>The cultivation of rice (Oryza sativa L.) has become increasingly challenging due to various abiotic stresses such as elevated salinity and drought conditions, and these challenges will only worsen with climate change. This review highlights recent advancements in CRISPR/Cas9 genome editing technology as applied to developing rice for greater tolerance of abiotic stresses. Functional genomics analysis (including transcriptomic, proteomic, and metabolomic data) has identified and validated key regulatory genes and networks that affect plant response to abiotic stress. Researchers using CRISPR/Cas9 technology have made changes to both negative regulators (e.g., OsRR22 for salinity; OsDST for drought/salt) and positive (e.g., OsSAPK2 for drought) regulatory genes, producing transgene-free mutants displaying enhanced ion homeostasis and ROS scavenging as well as stomatal regulation and stability of yield under stress. Results from functional genomics indicate that polygenic regulatory networks, including the transcriptional regulators (NAC, WRKY, bHLH) and abscisic acid (ABA) signalling systems, are suitable for multiplex applications to produce a plant with broad-based stress resilience. Although functional genomic methods have been successful in the lab, there are still major issues with performance under field conditions when multiple stresses are present (e.g., genotype dependence, japonica bias, pleiotropic effects, and regulatory issues). The future integration of prime-editing, network mapping, and multi-site trial work will assist in the development of sustainable, superior quality indica varieties to help fulfill the UN's Sustainable Development Goals (SDGs) for food security.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148302028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalization of Chitosan with Silver Oxide and Berberis aristata for Bioactive Composites with In Vivo Wound Healing Evaluation.","authors":"Zainab Khan, Saira Bibi, Wajid Rehman, Obaid-Ur-Rahman Abid, Mohsan Nawaz, Ashwag S Alanazi, Mona A Alamri, Masab Saeed, Liaqat Rasheed, Naqeeb Ullah Khan","doi":"10.1007/s12033-026-01587-w","DOIUrl":"https://doi.org/10.1007/s12033-026-01587-w","url":null,"abstract":"<p><p>This study focuses on the modification of chitosan (Cs) with silver oxide (Ag₂O) nanoparticles and Berberis aristata (B. aristata) (denoted as BA) extract to develop advanced wound dressing films. Cs/Ag₂O and Cs/Ag₂O/BA films were fabricated via solution casting with TEOS cross-linking, incorporating ultrasonically dispersed Ag₂O and BA extract under stirring, followed by structural, antimicrobial, and wound healing evaluation. Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Diffraction (XRD) analyses confirmed the successful incorporation of Ag₂O nanoparticles into the chitosan matrix, with characteristic shifts in the spectra and crystalline diffraction patterns. The antibacterial properties were assessed using the agar disk diffusion method, revealing that Cs/Ag₂O/BA-3% films exhibited the highest inhibition zones of 17 mm against Escherichia coli (E. coli) and 16 mm against Streptococcus aureus (S. aureus), indicating strong antimicrobial efficacy. The films also demonstrated excellent swelling behavior, with a maximum water absorption of over 60% and showed remarkable hydrolytic and oxidative stability, with Cs/Ag₂O/BA-3% exhibiting 19 and 82 days of stability, respectively. The enhanced swelling and stability indicate aptness for maintaining a moist wound environment, which is critical for rapid healing. In vivo wound healing studies conducted on a rabbit model showed that Cs/Ag₂O/BA-3% promoted the most significant wound closure, with 98% closure by Day 7, compared to Cs-blank (84%) and commercial gauze (77%). These results highlight the potential of Cs/Ag₂O/BA composites as effective, bioactive wound dressings with enhanced healing, antimicrobial, and stability properties, making them promising candidates for advanced wound care applications. In this context, and considering the worldwide increase in antibiotic-resistant infections and the growing need for bioactive wound dressings, these outcomes present a hopeful, affordable, and expandable approach to enhanced wound treatment. Hence, they show promising potential for application as bioactive wound dressings for infected and chronic wounds.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resilience in Tribolium Castaneum Through Antioxidant Priming Under Phosphine-Induced Oxidative Stress.","authors":"Mudagadde G Deeksha, Suresh M Nebapure, Doddachowdappa Sagar, Ramcharan Bhattacharya, Anil Dahuja, Sabtharishi Subramanian","doi":"10.1007/s12033-026-01586-x","DOIUrl":"https://doi.org/10.1007/s12033-026-01586-x","url":null,"abstract":"<p><p>This study investigates the effects of sublethal and high-dose phosphine exposure on biocatalytic activity and gene expression of key antioxidants (catalase, peroxidase, and superoxide dismutase) in T. castaneum populations: susceptible (LS), weakly resistant (WR), and strongly resistant (SR). Multigenerational bioassays demonstrated a marked increase in resistance, with WR and SR populations exhibiting resistance ratios exceeding 30-fold following repeated phosphine exposure. Biochemical analyses revealed a significant decline in catalase activity (1.28-2.25-fold) in WR and SR populations, whereas peroxidase activity increased substantially, particularly in SR populations exposed to sublethal (3.25-fold) and high-dose (3.45-fold) treatments. Gene expression analysis normalized to RPS18, showed downregulation of catalase transcripts in WR and SR populations, alongside pronounced upregulation of peroxidase and superoxide dismutase (SOD) genes with increasing resistance. Multiple regression analysis revealed a strong association (R² > 0.85) between resistance levels, antioxidant enzyme activities, and corresponding gene expression profiles. This study explores phosphine exposure, antioxidant defences, and resistance in T. castaneum, underscoring the need for continuous monitoring and adaptive management for sustainable resistance.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CMKLR1 as a Therapeutic Target: Vitamin D-Mediated Suppression of Inflammation and Oxidative Stress in SA-AKI.","authors":"Jingjing Wang, Rennan Guo, Lu Wang, Junchuan Guo, Boranyi Bayinchahan, Zhigao Wang, Dong Xiao","doi":"10.1007/s12033-025-01534-1","DOIUrl":"10.1007/s12033-025-01534-1","url":null,"abstract":"<p><p>Sepsis-associated acute kidney injury (SA-AKI) is a severe complication of sepsis, primarily driven by immune-inflammatory dysregulation and oxidative stress, and 1,25-Dihydroxyvitamin D₃ (1,25(OH)₂D₃) shows renoprotective potential, yet its molecular targets and mechanisms remain unclear. Transcriptomic data of SA-AKI from GEO were analyzed by ssGSEA for immune infiltration, differential expression analysis, WGCNA, and machine learning (LASSO, SVM-RFE, Random Forest) to identify the biomarkers. In vivo, SA-AKI was induced by cecal ligation and puncture (CLP) in mice treated with 1,25(OH)₂D_3, and renal function, histology, inflammatory cytokines, oxidative stress markers, and CMKLR1 expression were evaluated. In vitro, LPS-stimulated HK-2 cells were treated with 1,25(OH)₂D₃ with or without CMKLR1 overexpression to assess cytokines, cell viability, apoptosis, and oxidative stress. Four key biomarkers-CMKLR1, COL1A1, RPS19, and THBS1-were identified by machine learning, with CMKLR1 as the key target. In SA-AKI mice, 1,25(OH)₂D₃ downregulated CMKLR1, improved renal function, and reduced inflammation and oxidative stress. In LPS-stimulated HK-2 cells, it dose-dependently suppressed cytokine release, restored cell viability, and alleviated oxidative stress, while CMKLR1 overexpression partially reversed these effects. CMKLR1 was identified as a key immune inflammation target in SA-AKI. 1,25(OH)₂D₃ was protected against renal injury by suppressing CMKLR1, and mitigating inflammation, oxidative stress, and apoptosis, while CMKLR1 overexpression partially reversed these effects. These findings highlight CMKLR1 as a potential therapeutic target for SA-AKI.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2800-2819"},"PeriodicalIF":3.2,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13279403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}