Molecular Biotechnology最新文献

{"title":"DcMADS3, a MADS-Box Transcription Factor, Highly Expressed at the Middle Flower Bud Stage and Involved in Petalization Development in Carrot.","authors":"Shan-Shan Tan, Shan-Shan Liu, Jing Ma, Yi Liang, Ao-Qi Duan, Yuan-Jie Deng, Ya-Hui Wang, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong","doi":"10.1007/s12033-025-01489-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01489-3","url":null,"abstract":"<p><p>Carrot (Daucus carota), an Apiaceae family crop, possesses the typical compound umbellate inflorescence with small flowers. The mechanism of carrot floral development is still unknown. APETALA3 (AP3) is a MADS-box transcription factor that primarily participates in stamen and petal development. In this study, DcMADS3 belonging to the APETALA3 group was cloned, which can form a heterodimer with DcMADS2 belonging PI (PISTILLATA) group. Expression analysis revealed DcMADS3 was higher in 'Kurodagosun' (normal flower, KRD) than 'Deep Purple' ('petaloid' CMS phenotype, DPP) at the middle flower bud stage. Its expression was greater in DPP petals (normal petal and petaloid petal) than in KRD petals, suggesting its potential role in regulating petalization development in carrot. Transgenic Arabidopsis overexpressing DcMADS3 resulted in altered petal size, increased petal number, and altered flower symmetry. Furthermore, yeast one-hybrid (Y1H) library screening identified DcXTH-like (DCAR_009699) as a potential factor binding to the DcMADS3 promoter. These results provided a theoretical foundation for further investigation of carrot floral organ development mechanism.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of Real-Time Allele-Specific PCR-Based Genotyping of the rs1042713 Variant in the ADRB2 Gene and Its Correlation with Salbutamol Response.","authors":"Dilla Jose, N S Vincent","doi":"10.1007/s12033-025-01483-9","DOIUrl":"https://doi.org/10.1007/s12033-025-01483-9","url":null,"abstract":"<p><p>Asthma is a prevalent chronic respiratory disease influenced by genetic and environmental factors. The beta-2 adrenergic receptor (ADRB2) gene, located on chromosome 5q31-q32, plays a crucial role in bronchodilation by regulating airway smooth muscle relaxation through cyclic adenosine monophosphate (cAMP)-mediated signaling. Genetic variations in ADRB2, particularly + 46A > G (Arg16Gly, rs1042713) and + 79C > G (Gln27Glu, rs1042714), have been associated with altered receptor function, potentially impacting bronchodilator response to β2adrenergic receptor agonists such as salbutamol. Studies suggest that individuals with specific ADRB2 polymorphisms may exhibit variable drug efficacy, influencing asthma treatment outcomes. This study aims to investigate the association between ADRB2 polymorphism (Arg16Gly, rs1042713) and bronchodilator response, assessing their role in inter-individual variability in salbutamol efficacy. Allele-specific PCR (AS-PCR) employed to genotype these polymorphisms help in distinguishing homozygous and heterozygous variants. The findings could contribute to personalized asthma management, optimizing pharmacogenetic-based treatment strategies for improved therapeutic outcomes in a cost effective setting. Understanding the genetic basis of β2-AR variability may facilitate tailored asthma interventions, reducing adverse drug reactions and enhancing patient care.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of GPR75 Alleviates Lipid Metabolism by Activating the AMPK-SIRT1 Signaling Pathway In Vitro and In Vivo.","authors":"Junyu Wang, Guishun Sun, Shiwen Li, Xuan He, Rongzhuang Zou, Kunlin Li, Bian Wu","doi":"10.1007/s12033-025-01451-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01451-3","url":null,"abstract":"<p><p>The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is primarily driven by excessive lipid accumulation and metabolic dysregulation, necessitating a comprehensive investigation into the underlying mechanisms. This study employed an in vitro model, wherein Huh7 cells were induced with a palmitic acid/oleic acid mixture, and an in vivo model involving the provision of a high-fat diet to SD rats for six weeks. Employing techniques such as oil red O staining, immunofluorescence, and Western blotting, we examined lipid synthesis, metabolism, and the associated molecular pathways. The findings indicate that GPR75 overexpression markedly enhances lipid synthesis and impairs lipid metabolism. Conversely, GPR75 knockdown significantly diminished the fluorescence intensity of lipid synthesis factors FASN and SREBP1, concurrently elevating the expression of AMPK and SIRT1 proteins, which culminated in reduced lipid synthesis and improved lipid metabolism. Furthermore, inhibiting the AMPK-SIRT1 pathway following GPR75 knockdown led to a significant reversal of these lipid metabolic alterations. Overall, our study elucidates that GPR75 inhibition may diminish lipid accumulation and enhance lipid metabolism both in vitro and in vivo, primarily through the activation of the AMPK-SIRT1 signaling pathway.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Dual Cry1Ab and Cry1Ac Genes Under Regulation of MPI Promoter Enhances Resistance to Tomato Fruit Borer (Helicoverpa armigera) in Tomato.","authors":"Khadijeh Abbaszadeh, Reza Shirzadian-Khorramabad, Mohammad-Mehdi Sohani, Zahra Hajiahmadi","doi":"10.1007/s12033-025-01486-6","DOIUrl":"https://doi.org/10.1007/s12033-025-01486-6","url":null,"abstract":"<p><p>Genetic engineering provides the possibility of expressing pest-resistant genes in transgenic plants. The pyramiding gene in plants is suggested to be a sustainable method for addressing pest resistance. To enhance resistance to tomato fruit borer (Helicoverpa armigera), tomato plants were transformed by pPZPY122:MPI:cry1Ac and pPZPY122:MPI:cry1Ab recombinant constructs. Bioassays on larvae consuming the single- and dual-gene transgenic tomatoes revealed that the cry1Ab/cry1Ac transgenic tomatoes could significantly improve resistance to the pest compared to the control and single-gene transgenic tomatoes. Mortality rates for larvae in single-gene plants expressing cry1Ab and cry1Ac were 36% and 47%, respectively. The cry1Ab/cry1Ac transgenic tomatoes demonstrated a 51% and 36% reduction in damage compared to cry1Ab and cry1Ac, as well as a 29% and 19% increase in larval mortality. The catalase and superoxide dismutase enzyme levels in the larvae feeding on the cry1Ab/cry1Ac plants significantly increased compared to those on the single-gene Bt. The rise in antioxidant enzyme activity is likely associated with heightened levels of Bt toxin in the larvae. It is concluded that the simultaneous expression of the cry1Ac and cry1Ab genes in tomatoes produces a synergistic effect of the Cry protein, notably increasing its lethality against the tomato fruit borer.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the YCjN ABC Transporter in Escherichia coli: Role in Maltose and Ethidium Bromide Transport.","authors":"Yanhong Wang, Beibei Xu, Amro Abdelazez, Heba Abdel-Motaal, Qingpeng Liu, Lu Han, Ming Yang, Wenzhao Wang, Mohamed A Abd El-Aziz, Garsa Alshehry, Eman Algarni, Huda Aljumayi","doi":"10.1007/s12033-025-01475-9","DOIUrl":"10.1007/s12033-025-01475-9","url":null,"abstract":"<p><p>This study investigated the functional role of the ycjN gene in Escherichia coli, hypothesizing that its encoded protein, YcjN, functions as a carbohydrate transporter. Bioinformatics analysis confirmed YcjN as a solute-binding protein (SBP). The construction of a ΔycjN deletion strain revealed that ycjN is not essential for bacterial viability. Ethidium bromide (EB) accumulation and efflux assays demonstrated that both the overexpression strain (E. coli/pET28a-ycjN) and wild-type E. coli exhibited significantly higher intracellular EB fluorescence as compared to null-loaded and defective strains, indicating enhanced substrate uptake capacity. No significant differences in EB efflux rates were observed among the strains, confirming that ycjN encodes an inwardly directed uptake pump. Further experiments using ATPase inhibitors ortho-vanadate and CCCP revealed that the YcjNOPV transporter actively transports substrates via ATP hydrolysis, classifying it as an ATP-binding cassette (ABC) family transporter. Substrate specificity analysis using high-performance liquid chromatography (HPLC) and reducing sugar detection confirmed that YcjNOPV primarily transports maltose while also exhibiting glucoside transport activity. These findings establish the functional role of the ycjN gene in carbohydrate transport and provide a foundation for engineering applications involving ABC transporters.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different Distribution of Carotenoid Content in Periderm-Phloem-Xylem of Red Carrot Taproots.","authors":"Xue-Feng Peng, Ya-Hui Wang, Rong-Rong Zhang, Zi-Han Zhao, Ai-Sheng Xiong","doi":"10.1007/s12033-025-01487-5","DOIUrl":"https://doi.org/10.1007/s12033-025-01487-5","url":null,"abstract":"<p><p>The color of carrot taproots is mainly determined by the types and contents of carotenoids and anthocyanins. Consistency in taproot color from the outside to the inside is an important indicator of carrot quality. However, most carrot cultivars exhibit inconsistency in taproot color across these tissues. This difference is primarily due to variations in carotenoid content and types in non-purple carrot, though the underlying mechanism remains unclear. In this research, four red carrot cultivars 'Benhongjinshi' (BHJS), 'Dayumeirenzhi' (DY), 'Betafruit' (BT), and 'Meiguihong' (MGH) were analyzed for carotenoid content using high-performance liquid chromatography. The expression levels of genes involved in carotenoid biosynthesis were also examined. The results showed that the total carotenoid content varied among the tissues: in the periderm, 'BHJS' had 0.4244 mg/g, 'DY' 0.4955 mg/g, 'BT' 0.5229 mg/g, and 'MGH' 0.5925 mg/g; in the phloem, 'BHJS' had 0.5757 mg/g, 'DY' 0.6234 mg/g, 'BT' 0.2744 mg/g, and 'MGH' 0.3948 mg/g; in the xylem, 'BHJS' had 0.2917 mg/g, 'DY' 0.0807 mg/g, BT 0.1748 mg/g, and 'MGH' 0.1073 mg/g. The total carotenoid content was lowest in the xylem across all four red carrot cultivars. The expression levels of genes DcLCYB1, DcLCYB2, and DcCCD4, which regulate carotenoid metabolism, differed in the periderm, phloem, and xylem of carrot taproots. These differences in carotenoid type and content across the periderm, phloem, and xylem contribute to the inconsistent color of red carrot taproots from outside to inside. This phenomenon may result from the differential expression of structural genes regulating carotenoid metabolism in these tissues.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsion-Based Tea Tree Oil: A New Frontier in Enhancing Clotrimazole Antifungal Activity.","authors":"Fatemeh Khorsand, Seyed Jamal Hashemi, Shahram Mahmoudi, Hasti Kamali Sarvestani, Amir Amani","doi":"10.1007/s12033-025-01485-7","DOIUrl":"https://doi.org/10.1007/s12033-025-01485-7","url":null,"abstract":"<p><p>Clotrimazole is widely used to treat fungal diseases, but it should be applied topically for several weeks to be effective. Previous studies have shown that nanoemulsions can enhance the topical activity of various active ingredients. This study aimed to prepare a nanoformulation of clotrimazole to improve its antifungal activity. A nanoemulsion containing clotrimazole was prepared using Tween 80 and Tween 20. The particle size was measured using Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM). Stability studies were conducted using freeze-thaw cycles and storage at 40 °C. The antifungal activity of clotrimazole was then compared with bulk clotrimazole both in vitro and in vivo. The nanoemulsion containing clotrimazole had a particle size of 31.5 nm, measured by DLS and remained physically stable after 1 year of storage. In vitro antifungal studies showed that this nanoemulsion outperformed both bulk clotrimazole and the nanoemulsion without clotrimazole, with MIC values of 1/524288, 1/8192, and 1/1024, respectively, against T. mentagrophytes. In addition, animal studies demonstrated that mean (SD) treatment time for fungal infection was 8.6 (0.5) days with the nanoemulsion containing 1% clotrimazole, significantly smaller than that of bulk clotrimazole (1% w/w) and nanoemulsion without clotrimazole (i.e., 15.0 (1.6) and 20.0 (0.7) days, respectively). The treatment time was more than 21 days for the bulk tea tree oil and the untreated control. After 21 days of treatment, only animals treated with the clotrimazole nanoemulsion showed complete lesion healing and weight changes comparable to healthy animals. The nanoemulsion formulation containing clotrimazole demonstrates highly desirable antifungal properties, warranting further investigation in clinical trials.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic and Immunologic Characteristics of Head and Neck Squamous Cell Carcinoma Based on Disulfidptosis-Related lncRNAs.","authors":"Sai Liang, Xuan Yang, Ji Wang, Ming Yu, Zheng-Peng Gong","doi":"10.1007/s12033-025-01471-z","DOIUrl":"https://doi.org/10.1007/s12033-025-01471-z","url":null,"abstract":"<p><p>Disulfidptosis is a new and unique mode of cell death in malignant tumors that plays an important role in tumorigenesis and development. This study aimed to investigate the prognostic value of disulfideptosis-related lncRNAs (DRLs) in head and neck squamous cell carcinoma (HNSCC). Differential expression and Pearson correlation analyses were used to identify DRLs associated with HNSCC, and a regression model containing six core DRLs was constructed using univariate Cox regression analysis and least absolute shrinkage and selection operator (LASSO) regression analysis. The predictive power of the models was assessed using Kaplan-Meier analysis, receptor operating characteristic curves (ROC), principal component analysis (PCA), nomograms, and consistency indices. In addition, the relationship between the immune microenvironment of HNSCC and risk prognostic modeling was investigated by functional enrichment using Gene Ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), single-sample genome enrichment analysis (ssGSEA), and GSEA. Finally, potentially effective drugs for the treatment of HNSCC were identified by immune checkpoint and drug sensitivity screening. In addition, the expression of DRGs and DRLs was assessed using RT-qPCR and protein blotting analysis. A risk score model consisting of six DRLs (MIR34AHG, RAB11B-AS1, SNHG16, AC108693.2, AC090587.1, and AL590617.2) was constructed, which can be used to guide the prognosis and immune microenvironment of HNSCC. According to the survival analysis, high-risk patients had a poorer prognosis. GO and KEGG analyses showed enrichment of DRLs and immune-related pathways. The differential expression of disulfide-related genes (DRGs) and DRLs in HNSCC and adjacent normal tissues was further confirmed by qPCR and protein blotting analysis. Our study elucidated the relationship between DRLs and the prognosis, immune microenvironment, tumor mutation burden (TMB), and drug sensitivity of HNSCC and provided new ideas for individualized treatment of HNSCC.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a Phage Display-Selected scFv Against Neuropilin 1 (NRP1) Isolated from a Naïve Human Library.","authors":"Khadijeh Farrokhi, Fatemeh Nasiri, Pouya Safarzadeh Kozani, Pooria Safarzadeh Kozani","doi":"10.1007/s12033-025-01480-y","DOIUrl":"https://doi.org/10.1007/s12033-025-01480-y","url":null,"abstract":"<p><p>Immune checkpoint inhibitors are established therapeutics for solid tumors. This study isolated a single-chain fragment variable (scFv) targeting neuropilin 1 (NRP1), a promising therapeutic target. A naïve human scFv phage display library underwent five biopanning rounds against NRP1. The lead clone (KZC28) was expressed and characterized. Binding affinity was quantified via ELISA. Specificity was assessed by cross-reactivity testing against structurally similar/unrelated antigens. Sensitivity determined the lower detection limit. Cell-based ELISA and flow cytometry evaluated binding to endogenous NRP1 on U87-MG glioblastoma and PC3 prostate cancer cells. Computational modeling predicted structural stability, solubility, flexibility, and binding energetics, focusing on complementarity-determining region (CDR) interactions. KZC28 exhibited high affinity (K_d = 0.88 nM) and specificity, with no cross-reactivity despite high sequence identity. Sensitivity reached 20 ng/mL for NRP1 detection. Cell-based assays and flow cytometry confirmed robust binding to U87-MG and PC3 cells, with specific binding comparable to a commercial anti-NRP1 mAb and minimal binding to NRP1⁻ cells. In silico analyses predicted favorable solubility, structural flexibility, and energetically stable CDR-mediated binding. KZC28 demonstrates high specificity and affinity for NRP1 with promising biophysical and functional properties. These support its potential as a targeting moiety for solid tumor therapy, particularly in NRP1-overexpressing cancers. Further preclinical evaluation of in vivo efficacy is warranted.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significance of a Novel Angiogenesis-Related Biomarker Neuropilin-1 in Keloids.","authors":"Ming-Yuan Jin, Xiao-Ying Lin, Yong Wang, Ying Li, Tao Zhang, Ze-Ming Zhuang, Xin-Cao Zhong, Wei-Qiang Tan","doi":"10.1007/s12033-025-01481-x","DOIUrl":"https://doi.org/10.1007/s12033-025-01481-x","url":null,"abstract":"<p><p>Keloids are a severe type of pathological scars caused by excessive connective tissue hyperplasia after skin injury. Uncontrolled angiogenesis during wound healing can contribute to keloid formation, highlighting the importance of angiogenesis regulation in keloid prevention and treatment. In this study, angiogenesis-related biomarkers in keloids were identified using a combination of machine learning methods and weighted gene co-expression network analysis (WGCNA), based on gene expression data from the Gene Expression Omnibus (GEO) database. Neuropilin-1 (NRP1) was selected for further investigation and validated in clinical tissue samples using immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (RT-qPCR). Immune microenvironment analysis indicated a significant positive correlation between the NRP1 expression level and the abundance of immune and stromal cells, particularly endothelial cells. The biological role of NRP1 at the single-cell level revealed that keloid endothelial cells potentially promote collagen deposition in the extracellular matrix (ECM), which is closely associated with the keloid NRP1⁺ endothelial cells. In summary, NRP1 plays an essential role in the occurrence and development of keloids and represents a promising angiogenesis-related therapeutic target.</p>","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}