Molecular Biotechnology最新文献_第7页

Correlations of FCGR2A 131R/H and FCGR3A 158V/F Polymorphisms with the Susceptibility of Peri-implantitis in Chinese Han Population. 中国汉族人群中 FCGR2A 131R/H 和 FCGR3A 158V/F 多态性与种植体周围炎易感性的相关性

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-06-01 Epub Date: 2024-05-21 DOI: 10.1007/s12033-024-01193-8

Peng Li, Bingzhuo Chen, Liang Zhao, Feng Yang, Zhu Zhang, Yuan Cao, Yang Hu

引用次数: 0

Construction and Multi-dimensional Validation of a Lactylation-Related Signature for Glioblastoma Multiforme Prognostic and Therapeutic Purposes. 多形性胶质母细胞瘤预后和治疗目的的乳酸化相关标记的构建和多维验证。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-23 DOI: 10.1007/s12033-025-01446-0

Wenjie Wen, Jiongxue Chen, Fuyin Deng, Daji Guo, You Zuo, Xuewen Chen, Youjia Li, Yi Li, Yamei Tang

{"title":"Construction and Multi-dimensional Validation of a Lactylation-Related Signature for Glioblastoma Multiforme Prognostic and Therapeutic Purposes.","authors":"Wenjie Wen, Jiongxue Chen, Fuyin Deng, Daji Guo, You Zuo, Xuewen Chen, Youjia Li, Yi Li, Yamei Tang","doi":"10.1007/s12033-025-01446-0","DOIUrl":"https://doi.org/10.1007/s12033-025-01446-0","url":null,"abstract":"Glioblastoma multiforme, one of the most malignant types of brain tumor, heavily relies on glycolytic pathways and is significantly influenced by immune infiltration and its surrounding microenvironment. Growing evidence implies that increase in glycolysis can lead to lactate accumulation, which further contributed to histone lactylation, playing a crucial role in tumor development, maintenance, and therapeutic response. This study explores the prognostic and therapeutic potential of lactylation-related genes in glioblastoma multiforme. Using single-cell (GSE162631) and bulk transcriptome datasets (TCGA, CGGA, and GSE16011), we identified lactylation-related genes through ssGSEA and WGCNA. Moreover, a machine learning framework, incorporating 10 algorithms and 101 combinations, was used to establish an eight-gene lactylation-related signature (POLDIP3, MMP14, MDK, KDELR2, GSTK1, DEDD2, CD151, and BRI3) with robust predictive accuracy for patient survival. A nomogram with lactylation-related signature integration was developed as a quantitative prognostic instrument for clinical use. Moreover, patients classified by lactylation-related signature risk scores showed distinct immune status, tumor mutation burden, immunotherapy response, and drug sensitivity. The expression of those lactylation-related genes was further validated by quantitative PCR and functional experiment in normal and GBM cell lines. Overall, this study establishes a lactylation-related signature with significant potential for glioblastoma multiforme prognostic prediction, targeted prevention, and individualized therapy.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Carbonate Mineralization: A Comprehensive Review of Mechanisms, Applications, and Recent Advancements. 微生物碳酸盐矿化：机制、应用和最新进展综述。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-08 DOI: 10.1007/s12033-025-01433-5

Amiya Ojha, Tarun Kanti Bandyopadhyay, Deeplina Das, Palash Dey

{"title":"Microbial Carbonate Mineralization: A Comprehensive Review of Mechanisms, Applications, and Recent Advancements.","authors":"Amiya Ojha, Tarun Kanti Bandyopadhyay, Deeplina Das, Palash Dey","doi":"10.1007/s12033-025-01433-5","DOIUrl":"https://doi.org/10.1007/s12033-025-01433-5","url":null,"abstract":"Microbial carbonate mineralization, the process by which microorganisms (Bacillus sp., Sporosarcina sp., Penicillium sp., Cyanobacteria, etc.) directly mediate or indirectly influence mineral formation and deposition, represents the next frontier in technology with vast potential across scientific disciplines, including construction, environmental remediation, and carbon sequestration. This review explores the fundamental aspects of microbial carbonate mineralization, focusing on key mechanisms such as photosynthesis, methane oxidation, sulfate reduction, ureolysis, denitrification, carbonic anhydrase activity, iron reduction, and EPS mediation, all of which influence carbonate saturation and mineral nucleation. Additionally, it highlights critical regulatory factors that enhance biomineralization for bio-inspired material development in heavy metal remediation, wastewater treatment, self-healing concrete, biomedical applications, nanoscale technologies, and 3D printing. A major focus is microbial-induced calcite precipitation (MICP), an emerging and cost-efficient biomineralization technique, with an in-depth analysis of its molecular mechanisms and expanding applications. Furthermore, this review discusses current challenges, including process scalability, long-term stability, and environmental and safety considerations, while identifying future research directions to improve the efficacy and sustainability of microbial carbonate mineralization in advanced technological applications.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver Nanoparticles as Antibacterials: Synthesis, Characterization, and Molecular Simulations Against β-Lactamase-Producing Enterobacter and Salmonella spp. 银纳米粒子作为抗菌剂：合成、表征和对抗产β-内酰胺酶肠杆菌和沙门氏菌的分子模拟。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-06 DOI: 10.1007/s12033-025-01445-1

Khattab Al-Khafaji, Alhamza D Hameed, Marwah Shuwaili

{"title":"Silver Nanoparticles as Antibacterials: Synthesis, Characterization, and Molecular Simulations Against β-Lactamase-Producing Enterobacter and Salmonella spp.","authors":"Khattab Al-Khafaji, Alhamza D Hameed, Marwah Shuwaili","doi":"10.1007/s12033-025-01445-1","DOIUrl":"https://doi.org/10.1007/s12033-025-01445-1","url":null,"abstract":"Multidrug resistance poses a threat to public health all over the world. Evidences suggest that third-generation antibiotic resistance mediated by extended-spectrum beta-lactamases (ESBLs) in all Enterobacteriaceae species, especially in Enterobacter and Salmonella. In this study, silver nanoparticles (AgNPs) were synthesized and characterized using sol-gel synthesis and powder X-ray diffraction. It was shown that clinical isolates were sensitive to AgNPs. The MIC and MBC, time-dependent growth inhibition test as well as well diffusion agar techniques. ESBL generation was examined using these approaches. There was a low MIC value of 500 μg/ml for Enterobacter and Salmonella. 1000 μg/ml of AgNPs inhibited the development of microorganisms. The antibacterial effect of AgNPs was slow but dependent on concentration and duration. At a concentration of 100 μg/ml, the inhibition zone for Enterobacter was 22 mm, whereas that for Salmonella was 20 mm. Further, molecular docking employed to explore the binding affinity between AgNPs and the active site of beta-lactamase and compare it with reference. Results revealed a very strong score (- 26.79 kcal/mol). Next, MD simulation was performed. The MD simulation results showed a stable interaction between beta-lactamase-nanocluster. Experimental and computational results elucidate the molecular mechanism of anti-bacterial activity of AgNPs to fight against bacterial infections.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Insights into the Roles of PEST-Containing Nuclear Protein. 对含 PEST 核蛋白作用的最新认识。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-19 DOI: 10.1007/s12033-024-01188-5

Shiyun Guo, Ruidong Ding, Qian Zhao, Xu Wang, Shuangyu Lv, Xin-Ying Ji

引用次数: 0

Nogo-B Silencing Expedites the Senescence of Platelet-Derived Growth Factor-BB-Induced Human Hepatic Stellate Cells Via Autophagy. Nogo-B沉默可通过自噬加速血小板衍生生长因子-BB诱导的人肝星状细胞的衰老

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-10 DOI: 10.1007/s12033-024-01179-6

Lili Gao, Yingjie Zhuang, Zhengyi Liu

{"title":"Nogo-B Silencing Expedites the Senescence of Platelet-Derived Growth Factor-BB-Induced Human Hepatic Stellate Cells Via Autophagy.","authors":"Lili Gao, Yingjie Zhuang, Zhengyi Liu","doi":"10.1007/s12033-024-01179-6","DOIUrl":"10.1007/s12033-024-01179-6","url":null,"abstract":"Liver fibrosis is a severe liver pathology in response to chronic or iterative liver injury. Senescence has emerged as a protective mechanism against liver fibrosis. Nogo-B has been well established as a significant contributor to liver fibrosis. Nonetheless, researches regarding the role of Nogo-B in cell senescence during liver fibrosis are few. In platelet-derived growth factor-BB (PDGF-BB)-treated human hepatic stellate cell line LX-2, cell proliferation was assayed by CCK-8 method. Western blotting estimated the expression of Nogo-B and fibrosis markers. After Nogo-B was silenced in LX-2 cells pretreated by an autophagy activator Rapamycin and PDGF-BB, CCK-8 method was used to assess cell proliferation. Fibrosis was measured by western blotting and immunofluorescence. Cell cycle was subjected to flow cytometry analysis and cell senescence was evaluated by SA-β-gal staining. Immunofluorescence staining assessed autophagy. Nogo-B was elevated in PDGF-BB-exposed LX-2 cells. Nogo-B silencing suppressed the proliferation, fibrosis, and autophagy while induced cell cycle arrest and senescence of LX-2 cells. Additionally, pretreatment with Rapamycin partially restored the effects of Nogo-B knockdown on the autophagy, proliferation, fibrosis, cell cycle, and senescence of LX-2 cells upon exposure to PDGF-BB. Collectively, inactivation of autophagy mediated by Nogo-B deficiency might elicit protective activities against the development of liver fibrosis.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2023-2034"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of PLK1 Molecule Following Incomplete Thermal Ablation Promotes the Proliferation and Invasion of Residual Hepatocellular Carcinoma. 不完全热消融后 PLK1 分子的过表达促进残余肝细胞癌的增殖和侵袭

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-23 DOI: 10.1007/s12033-024-01181-y

Tong Kang, Jiamin Chen, Weijun Wan, Jinshu Pang, Rong Wen, Xiumei Bai, Lipeng Li, Yunjing Pan, Yun He, Hong Yang

{"title":"Overexpression of PLK1 Molecule Following Incomplete Thermal Ablation Promotes the Proliferation and Invasion of Residual Hepatocellular Carcinoma.","authors":"Tong Kang, Jiamin Chen, Weijun Wan, Jinshu Pang, Rong Wen, Xiumei Bai, Lipeng Li, Yunjing Pan, Yun He, Hong Yang","doi":"10.1007/s12033-024-01181-y","DOIUrl":"10.1007/s12033-024-01181-y","url":null,"abstract":"TAT, a widely used treatment for HCC, can exacerbate the progression of residual HCC. The present study investigated the mechanism of action of PLK1 following ITA of HCC. The PLK1 levels in HCC were determined using qRT-PCR from clinical patient samples, IHC from tissue microarray, and data from globally high-throughput data and microarrays. The PLK1 levels and their effect on the biological phenotype of heat-stress HCC cells were evaluated through in vitro experiments. We detected PLK1 abnormal expression in HCC models of nude mice subjected to ITA. We detected the effects of different PLK1 expression levels on EMT pathway proteins. PLK1 exhibited an overexpression in HCC tissues with an SMD of 1.19 (3414 HCC and 3036 non-HCC tissues were included), distinguishing HCC from non-HCC effectively (AUC = 0.9). The qRT-PCR data from clinical HCC patient samples and IHC from HCC tissue microarray results also indicated an overexpressed level. In the incomplete ablation models, an increased PLK1 expression was found in both heat-stress cells and subcutaneous tumors. The upregulation of PLK1 following ITA was found to enhance the malignancy of HCC and exacerbate the proliferation, migration, and invasion of residual HCC cells, whereas PLK1 knockdown suppressed the biological malignancy of HCC cells. Meanwhile, PLK1 has different regulatory effects on various EMT pathway proteins. PLK1 promotes the progression of residual HCC by activating EMT pathway after ITA, which might provide a novel idea for the treatment and prognosis of residual HCC.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"2046-2059"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Molecular Landscape of Neutrophil Extracellular Traps in Severe Asthma: Identification of Biomarkers and Molecular Clusters. 揭示严重哮喘中中性粒细胞胞外陷阱的分子图谱：生物标记物和分子集群的鉴定。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-05-27 DOI: 10.1007/s12033-024-01164-z

Kunlu Shen, Jiangtao Lin

{"title":"Unraveling the Molecular Landscape of Neutrophil Extracellular Traps in Severe Asthma: Identification of Biomarkers and Molecular Clusters.","authors":"Kunlu Shen, Jiangtao Lin","doi":"10.1007/s12033-024-01164-z","DOIUrl":"10.1007/s12033-024-01164-z","url":null,"abstract":"Neutrophil extracellular traps (NETs) play a central role in chronic airway diseases. However, the precise genetic basis linking NETs to the development of severe asthma remains elusive. This study aims to unravel the molecular characterization of NET-related genes (NRGs) in severe asthma and to reliably identify relevant molecular clusters and biomarkers. We analyzed RNA-seq data from the Gene Expression Omnibus database. Interaction analysis revealed fifty differentially expressed NRGs (DE-NRGs). Subsequently, the non-negative matrix factorization algorithm categorized samples from severe asthma patients. A machine learning algorithm then identified core NRGs that were highly associated with severe asthma. DE-NRGs were correlated and subjected to protein-protein interaction analysis. Unsupervised consensus clustering of the core gene expression profiles delineated two distinct clusters (C1 and C2) characterizing severe asthma. Functional enrichment highlighted immune-related pathways in the C2 cluster. Core gene selection included the Boruta algorithm, support vector machine, and least absolute contraction and selection operator algorithms. Diagnostic performance was assessed by receiver operating characteristic curves. This study addresses the molecular characterization of NRGs in adult severe asthma, revealing distinct clusters based on DE-NRGs. Potential biomarkers (TIMP1 and NFIL3) were identified that may be important for early diagnosis and treatment of severe asthma.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1852-1866"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-361-5p Alleviates Leydig Cell Apoptosis and Promotes Cell Growth by Targeting PIAS1 in Late-Onset Hypogonadism. MicroRNA-361-5p通过靶向PIAS1缓解晚发性性腺功能减退症患者的精原细胞凋亡并促进细胞生长

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-06-11 DOI: 10.1007/s12033-024-01174-x

Xunrong Zhou, Chunsheng Ben, Dong Wu, Anle Xia, Ping'an Chang, Bin He, Ninghan Feng, Cheng Wu

{"title":"MicroRNA-361-5p Alleviates Leydig Cell Apoptosis and Promotes Cell Growth by Targeting PIAS1 in Late-Onset Hypogonadism.","authors":"Xunrong Zhou, Chunsheng Ben, Dong Wu, Anle Xia, Ping'an Chang, Bin He, Ninghan Feng, Cheng Wu","doi":"10.1007/s12033-024-01174-x","DOIUrl":"10.1007/s12033-024-01174-x","url":null,"abstract":"Late-onset hypogonadism (LOH) is an age-related syndrome characterized by deficiency of serum testosterone produced by Leydig cells. Previous evidence suggested that microRNA (miR)-361-3p can serve as a promising biomarker for LOH. Nonetheless, its detailed function and molecular mechanism in LOH remain unclarified. The 24-month-old male mice were selected as an animal LOH model, and mouse Leydig cell line TM3 was stimulated with H2O2. ELISA was employed for testosterone level evaluation. Hematoxylin-eosin staining was implemented for histologic analysis of mouse testicular tissues. Western blotting and RT-qPCR were utilized for evaluating molecular protein and RNA expression, respectively. Functional experiments were conducted to test miR-361-5p roles. Luciferase reporter assay was for verifying the interaction between miR-361-5p and protein inhibitor of activated STAT 1 (PIAS1). miR-361-5p displayed a decreased level in the testes of LOH mice. Overexpressing miR-361-5p attenuated Leydig cell loss in the testis and elevated serum and intratesticular testosterone levels in LOH mice. H2O2 stimulation impaired TM3 cell viability, proliferation and intracellular testosterone production and enhanced cell apoptosis. miR-361-5p targeted PIAS1 in TM3 cell. PIAS1 upregulation counteracted miR-361-5p overexpression-mediated alleviation of cell apoptosis and elevation of testosterone synthesis in H2O2-stimualetd TM3 cells. miR-361-5p ameliorates LOH progression by increasing testosterone production and alleviate Leydig cell apoptosis via downregulation of PIAS1.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1968-1977"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of ROS and Regulatory Cell Death in Myocardial Ischemia-Reperfusion Injury. 处理心肌缺血再灌注损伤中的 ROS 和调节性细胞死亡。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-05-01 Epub Date: 2024-06-09 DOI: 10.1007/s12033-024-01173-y

Ge Gong, Wenhui Wan, Xinghu Zhang, Xiangxuan Chen, Jian Yin

{"title":"Management of ROS and Regulatory Cell Death in Myocardial Ischemia-Reperfusion Injury.","authors":"Ge Gong, Wenhui Wan, Xinghu Zhang, Xiangxuan Chen, Jian Yin","doi":"10.1007/s12033-024-01173-y","DOIUrl":"10.1007/s12033-024-01173-y","url":null,"abstract":"Myocardial ischemia-reperfusion injury (MIRI) is fatal to patients, leading to cardiomyocyte death and myocardial remodeling. Reactive oxygen species (ROS) and oxidative stress play important roles in MIRI. There is a complex crosstalk between ROS and regulatory cell deaths (RCD) in cardiomyocytes, such as apoptosis, pyroptosis, autophagy, and ferroptosis. ROS is a double-edged sword. A reasonable level of ROS maintains the normal physiological activity of myocardial cells. However, during myocardial ischemia-reperfusion, excessive ROS generation accelerates myocardial damage through a variety of biological pathways. ROS regulates cardiomyocyte RCD through various molecular mechanisms. Targeting the removal of excess ROS has been considered an effective way to reverse myocardial damage. Many studies have applied antioxidant drugs or new advanced materials to reduce ROS levels to alleviate MIRI. Although the road from laboratory to clinic has been difficult, many scholars still persevere. This article reviews the molecular mechanisms of ROS inhibition to regulate cardiomyocyte RCD, with a view to providing new insights into prevention and treatment strategies for MIRI.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1765-1783"},"PeriodicalIF":2.4,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141293576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0