Molecular Biotechnology最新文献_第6页

Development and Validation of a Highly Sensitive RT-qLAMP Assay for Rapid Detection of SARS-CoV-2: Methodological Aspects. 用于快速检测 SARS-CoV-2 的高灵敏度 RT-qLAMP 分析法的开发与验证：方法学方面。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-09-01 Epub Date: 2024-09-24 DOI: 10.1007/s12033-024-01275-7

Faezeh Mahmoudi, Davod Jafari, Seyedeh Mona Mousavi Esfahani, Arshad Hoseini, Mahmood Barati, Neda Saraygord-Afshari

{"title":"Development and Validation of a Highly Sensitive RT-qLAMP Assay for Rapid Detection of SARS-CoV-2: Methodological Aspects.","authors":"Faezeh Mahmoudi, Davod Jafari, Seyedeh Mona Mousavi Esfahani, Arshad Hoseini, Mahmood Barati, Neda Saraygord-Afshari","doi":"10.1007/s12033-024-01275-7","DOIUrl":"10.1007/s12033-024-01275-7","url":null,"abstract":"Specific and reliable diagnostic methods are becoming increasingly essential to identify patients in light of the high transmission rate and the recent appearance of the new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For the specific detection of SARS-CoV-2, our quantitative reverse transcription loop-mediated isothermal amplification (RT-qLAMP) assay implementation demonstrates how flexible it can be with two readouts: visualized colorimetric and real-time fluorescence. Different factors were optimized to improve the reaction conditions, including temperature (60 °C), assay runtime (60 min), primers, MgSO4 (6 mM), dNTPs (1 mM), LAMP Buffer (1.2 mM Tris-HCl), KCl (50 mM), pH (8), and phenol red (10 mM) concentrations. Regarding analytical sensitivity, the colorimetric RT-LAMP method detected samples with Ct values up to 29, while the RT-qLAMP assay identified up to Ct = 31. RT-qLAMP was evaluated on 40 clinical samples (25 positives and 15 negatives) for viral RNA detection. All negative samples were found negative through fluorescent reading in RT-qLAMP and quantitative reverse transcription PCR (RT-qPCR) assays. Twenty-three clinically positive samples demonstrated a positive RT-qLAMP reaction (up to Ct ≤ 31) with 92% clinical sensitivity, 100% clinical specificity, 100% positive predictive value (PPV), 88.24% negative predictive values (NPV), and 95% accuracy.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3696-3707"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Bibliometric and Visual Analysis of EGR1 in Cardiovascular Disease. 心血管疾病中EGR1的综合文献计量学和视觉分析。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-29 DOI: 10.1007/s12033-025-01493-7

Na Dong, Hongmei Yue

{"title":"A Comprehensive Bibliometric and Visual Analysis of EGR1 in Cardiovascular Disease.","authors":"Na Dong, Hongmei Yue","doi":"10.1007/s12033-025-01493-7","DOIUrl":"https://doi.org/10.1007/s12033-025-01493-7","url":null,"abstract":"Early Growth Response 1 (EGR1) is a crucial transcription factor that regulates diverse cellular processes, including growth, differentiation, proliferation, inflammation, apoptosis, and autophagy. It plays a vital role in maintaining cardiac homeostasis and is deeply implicated in the pathological mechanisms leading to cardiomyocyte death, making it a promising therapeutic target for cardiovascular diseases. To better understand the research landscape of EGR1 in this context, we conducted a bibliometric analysis using data from the Web of Science Core Collection (WoSCC) covering the period from 2014 to 2024, retrieved on 31 December 2024. A total of 2,112 publications related to EGR1 were identified, of which 173 specifically addressed cardiovascular disease and were published across 126 journals. Global research output on this topic has shown fluctuating growth over the past decade, with China contributing the most publications and citations. The Institut national de la santé et de la recherche médicale (Inserm) emerged as the most productive institution. Zhang Y authored the highest number of papers, while Wang J received the most citations. Scientific Reports was the most prolific journal, whereas the Journal of Biological Chemistry was the most influential in terms of citation metrics. Keyword co-occurrence and cluster analysis revealed major research themes such as apoptosis, inflammation, oxidative stress, angiogenesis, and autophagy. These findings provide a comprehensive overview of EGR1-related cardiovascular research and offer a valuable reference for future investigations into its mechanisms and therapeutic potential.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Human STARD3 Cholesterol Transporter Expression for Enhancing Bovine P450scc-Mediated Steroid Biotransformation in E. coli. 优化人STARD3胆固醇转运蛋白表达促进牛p450scc介导的类固醇在大肠杆菌中的生物转化。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-22 DOI: 10.1007/s12033-025-01490-w

Sofia V Zamalutdinova, Ludmila V Isaeva, Sergei A Golyshev, Mikhail A Rubtsov, Ludmila A Novikova

{"title":"Optimization of Human STARD3 Cholesterol Transporter Expression for Enhancing Bovine P450scc-Mediated Steroid Biotransformation in E. coli.","authors":"Sofia V Zamalutdinova, Ludmila V Isaeva, Sergei A Golyshev, Mikhail A Rubtsov, Ludmila A Novikova","doi":"10.1007/s12033-025-01490-w","DOIUrl":"https://doi.org/10.1007/s12033-025-01490-w","url":null,"abstract":"This study investigates the optimization of human STARD3 cholesterol transporter expression to enhance bovine P450scc-mediated steroid biotransformation in Escherichia coli, co-expressing STARD3 and P450scc system proteins (cytochrome P450scc (CYP11A1), adrenodoxin and adrenodoxin reductase). We compared different expression strategies for STARD3, including the use of pelB and TorA signal peptides for potential periplasmic localization, and evaluated different E. coli strains to maximize cholesterol biotransformation. Although both signal peptides failed to efficiently transport STARD3 to the periplasm, with most of the protein remaining in the cytoplasm and membrane fractions, the STARD3 protein retained functionality in cholesterol transport. Auto-induction with lactose proved superior to IPTG-based induction, resulting in significantly higher expression levels of both STARD3 and P450scc. Among the E. coli strains tested (BL21(DE3), BL21(DE3)pLysS, HMS174(DE3), C41(DE3), and Rosetta(DE3)pLysS), Rosetta(DE3)pLysS demonstrated the highest STARD3 expression and the most significant impact of STARD3 on cholesterol biotransformation. It increased P450scc activity by approximately three times in Rosetta(DE3)pLysS compared to strains expressing only the P450scc system proteins. This strain showed a cholesterol biotransformation efficiency that was 6.97 ± 3.69 times higher compared to the previously used BL21(DE3). These results provide valuable insights for the optimization of recombinant steroidogenic systems in bacterial hosts for potential biotechnological applications.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in CRISPR-Cas Systems for Genome Editing towards Eradication of Human Microbial Pathogens. CRISPR-Cas基因编辑系统在根除人类微生物病原体方面的进展

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-20 DOI: 10.1007/s12033-025-01482-w

Gargi Bhattacharjee, Nisarg Gohil, Khushal Khambhati, Karan Murjani, Dinh Toi Chu, Nhat Le Bui, Hue Vu Thi, Indra Mani, Abhisheka Bansal, Sasanala Shamili, Lakkakula Satish, Suresh Ramakrishna, Khalid J Alzahrani, Vijai Singh

引用次数: 0

GALNT18-Mediated O-Glycosylation Promotes Development of Nonalcoholic Steatohepatitis-Associated Hepatocellular Carcinoma Through Activation of EGR1/TGF-β1/Smad Signaling. galnt18介导的o糖基化通过激活EGR1/TGF-β1/Smad信号促进非酒精性脂肪性肝炎相关肝细胞癌的发展

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-15 DOI: 10.1007/s12033-025-01494-6

Dan Chen, Ying Nie, Li Shi, Dingfu Zhong, Jiajia Wu, Bei Jin, Dong Liu

{"title":"GALNT18-Mediated O-Glycosylation Promotes Development of Nonalcoholic Steatohepatitis-Associated Hepatocellular Carcinoma Through Activation of EGR1/TGF-β1/Smad Signaling.","authors":"Dan Chen, Ying Nie, Li Shi, Dingfu Zhong, Jiajia Wu, Bei Jin, Dong Liu","doi":"10.1007/s12033-025-01494-6","DOIUrl":"https://doi.org/10.1007/s12033-025-01494-6","url":null,"abstract":"Nonalcoholic steatohepatitis-associated hepatocellular carcinoma (NASH-HCC) is an increasing global health issue. Research indicates that amyloid precursor protein (APP) might be a diagnostic marker and therapeutic target for HCC. However, the specific role of O-glycosylation of APP in NASH-HCC remains unclear. A NASH-HCC mice model was established by feeding BALB/c inbred mice with a high-fat/high-cholesterol (HFHC) diet and injection of CCl4. Hematoxylin-Eosin (HE) and Oil Red O (ORO) staining of liver tissue were then carried out to evaluate the progression of NASH-HCC. Immunohistochemistry (IHC) and Western blot were utilized to analyze APP levels. Immunoprecipitation (IP) was used to analyze the O-GalNAc modification of APP, and co-immunoprecipitation (Co-IP) and immunofluorescence were used to assess the Polypeptide N-Acetylgalactosaminyltransferase 18 (GALNT18) binding level of APP. Moreover, cell experiments confirmed the functional role of APP in HCC. APP was studied in vivo using an animal model. An up-regulation of APP and its O-glycosylation modification in patient-derived NASH-HCC tissues, animal models, and HCC cell lines was observed. The typical malignant phenotype of HCC, such as proliferation, migration, and invasion, was significantly suppressed by the knockdown of APP and was then partially rescued by the addition of UDP-GalNAc, a glycosylation activator. We demonstrated that GALNT18 interacted with APP in NASH-HCC models and upregulated its O-glycosylation. APP was finally shown to activate the EGR1/TGF-β1/Smad signaling, thereby driving the oncogenic progression of HCC. In vitro, APP knockdown inhibited NASH-HCC progression. This study identifies GALNT18-mediated O-glycosylation of APP as crucial in NASH-HCC development via the EGR1/TGF-β1/Smad pathway, suggesting that targeting GALNT18-APP signaling could be a therapeutic approach against NASH-HCC.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144855820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Description of Multicopy Recombinant Hepatitis B Surface Antigen: From Expression to Immune Analysis. 多拷贝重组乙型肝炎表面抗原的描述：从表达到免疫分析。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-08 DOI: 10.1007/s12033-025-01492-8

Özlem Bakangil, Elif Karaaslan, Sevde Hasanoğlu Sayın, Sercan Keskin, Mehmet Ziya Doymaz

{"title":"Description of Multicopy Recombinant Hepatitis B Surface Antigen: From Expression to Immune Analysis.","authors":"Özlem Bakangil, Elif Karaaslan, Sevde Hasanoğlu Sayın, Sercan Keskin, Mehmet Ziya Doymaz","doi":"10.1007/s12033-025-01492-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01492-8","url":null,"abstract":"Hepatitis B virus (HBV) remains a significant global health challenge. Although antiviral treatments have improved, vaccines containing the Hepatitis B surface antigen (HBsAg) are still the most effective prevention method. Since 1998, HBV vaccines have been part of Turkiye's mandatory childhood vaccination program, with all vaccines currently imported. This study primarily aims to optimize recombinant HBsAg production in Pichia pastoris and evaluate its immunological comparability to the commercial HBV vaccine, thereby assessing its potential as a locally produced vaccine candidate. To achieve this, an eight-copy tandem construct of the HBsAg gene was created on the pPICZαA plasmid through an in vitro multimerization, and the production process of recombinant HBsAg was optimized. The presence of HBsAg in the supernatants following induction was confirmed, and the antigen was purified using various physical and biochemical methods. The highest yield of recombinant HBsAg was achieved with the eight-copy construct, following induction with 1% methanol and harvesting at 120 h, which was identified as the most productive time point. The antigenic properties of the produced HBsAg were compared with those of the commercially available vaccine, Engerix B™. Immunological testing using serum from anti-HBsAg positive individuals and immunized animals demonstrated that the recombinant rHBsAg exhibited similar antigenic characteristics to the commercial vaccine. The findings of this study indicate that the recombinant HBsAg exhibits immunological properties comparable to the imported vaccine and suggest that it may be a potential candidate for future vaccine development.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144804377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Analysis and Biomineralization Efficacy of Bacillus megaterium SS3 for Improving Durability Properties of Building Material. 巨芽孢杆菌SS3提高建筑材料耐久性的基因组分析及生物矿化效果。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-07 DOI: 10.1007/s12033-025-01491-9

Bhavdeep Sharma, Shruti Sharma, Krishna M Medicherla, Sudhakara M Reddy

{"title":"Genomic Analysis and Biomineralization Efficacy of Bacillus megaterium SS3 for Improving Durability Properties of Building Material.","authors":"Bhavdeep Sharma, Shruti Sharma, Krishna M Medicherla, Sudhakara M Reddy","doi":"10.1007/s12033-025-01491-9","DOIUrl":"https://doi.org/10.1007/s12033-025-01491-9","url":null,"abstract":"Urease-producing microorganisms play an important role in biomineralization through microbially induced calcium carbonate precipitation (MICCP), contributing to enhanced durability and extended lifespan of construction materials in civil engineering. This study investigates the MICCP capabilities of a ureolytic strain, Bacillus megaterium SS3, isolated from alkaline calcareous soil, which showed native adaptation to high-pH environments typical of cementitious materials. Bacillus megaterium exhibited maximum urease activity (625 U/mL) and promoted CaCO3 precipitation up to 177.3 mg/100 mL. Its incorporation into cement mortar enhanced compressive strength by 18.9% and 10.58% at 7 and 28 days of curing, respectively, and significantly reduced water absorption compared to control specimens. Whole-genome sequencing and gene annotation revealed three structural urease genes (ureA, ureB, ureC) and four accessory urease genes (ureD, ureE, ureF, ureG), providing molecular insight into its biomineralization potential. To validate structure-function relationships, urease enzyme was modelled and molecular docking was performed with urea. The predicted structure showed strong binding at the catalytic site with key residues and nickel ions, confirming enzymatic suitability for MICCP. Bacillus megaterium SS3 not only exhibits effective MICCP performance but also showed enhanced environmental resilience when incorporated into mortar structures, positioning it as a strong candidate for microbial biocementation in civil engineering applications. This is the first report to integrate genome annotation, protein docking, and real-world application in mortar, positioning B. megaterium SS3 as a novel, genome-validated, biomineralizing strain for sustainable construction.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Cancers with Microrobots and Bacteriobots. 用微型机器人和细菌机器人治疗癌症。

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-06 DOI: 10.1007/s12033-025-01488-4

Aishani Pal, Ruchi Sonowane, Wusirika Ramakrishna

引用次数: 0

Genetic Transformation of Catharanthus roseus with Simplified Nanocarrier-Based Gene Delivery Method Using Green Synthesized Superparamagnetic Iron Oxide Nanoparticles. 绿色合成超顺磁性氧化铁纳米颗粒简化纳米载体基因传递方法对长春花的遗传转化

IF 2.5 4区生物学

Molecular Biotechnology Pub Date : 2025-08-04 DOI: 10.1007/s12033-025-01484-8

Sara Abedini, Shahram Pourseyedi, Jafar Zolala, Masoud Torkzadeh-Mahani, Roohollah Abdolshahi

{"title":"Genetic Transformation of Catharanthus roseus with Simplified Nanocarrier-Based Gene Delivery Method Using Green Synthesized Superparamagnetic Iron Oxide Nanoparticles.","authors":"Sara Abedini, Shahram Pourseyedi, Jafar Zolala, Masoud Torkzadeh-Mahani, Roohollah Abdolshahi","doi":"10.1007/s12033-025-01484-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01484-8","url":null,"abstract":"Conventional methods of plant genetic engineering have achieved significant successes but are often limited by species specificity and the need for specialized equipment. Recent advancements in nanotechnology-based gene delivery, particularly the use of nanocarriers, offer promising alternatives with enhanced efficiency, improved biocompatibility, and protection of exogenous nucleic acids. We developed a novel gene delivery approach, Simplified Nanocarrier-based Genetic Transformation (SNGT), using green-synthesized superparamagnetic iron oxide nanoparticles (gSION) for efficient DNA transfer into Catharanthus roseus leaf cells. Unlike previous green-synthesized nanoparticles primarily applied in biomedical contexts, this study explores their application in plant genetic engineering for the first time. We successfully synthesized gSION using aqueous extracts of C. roseus leaves. Following synthesis and physicochemical characterization, gSION-based nanocarriers loaded with the mGFP5-ER-encoding plasmid were prepared. Using this nanocarrier system, C. roseus leaves were successfully transformed. This method demonstrated effective gene delivery and transient gene expression in plant tissue.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture Optimization to Produce High Yields of Mycosporine-Like Amino Acids by Fischerella sp. F5. Fischerella sp. F5高产霉菌素样氨基酸的培养优化

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-08-01 Epub Date: 2023-08-19 DOI: 10.1007/s12033-023-00854-4

Shayan Salehian, Melika Saadatbakht, Maryam Tabarzad, Tahereh Hosseinabadi

{"title":"Culture Optimization to Produce High Yields of Mycosporine-Like Amino Acids by Fischerella sp. F5.","authors":"Shayan Salehian, Melika Saadatbakht, Maryam Tabarzad, Tahereh Hosseinabadi","doi":"10.1007/s12033-023-00854-4","DOIUrl":"10.1007/s12033-023-00854-4","url":null,"abstract":"Fischerella sp. is a valuable source of active metabolites, including UV-protecting compounds, among which mycosporin-like amino acids (MAAs) can be mentioned. Mycosporine-like amino acids are attractive secondary metabolites of a wide range of microorganisms, including microalgae and cyanobacteria. Enhanced production of MAAs has been studied in different sources. This study aimed to optimize the phosphate and nitrate concentrations of the culture medium on BG11 to maximize MAAs production from Fischerella sp. F5, using response surface methodology. The extraction process from the cultures, grown in adjusted conditions, was also optimized. The results confirmed that increasing both, nitrate and phosphate concentration, in the culture medium had a positive effect on the MAAs production by Fischerella sp. F5. While, optimization of the extraction process was not led to a highly accurate predictive model; temperature, sonication time, methanol ratio, and solvent/biomass ratio exhibited significant effects on the final MAAs' concentration in partially purified extracts. In general, more optimization cultures studies need to complete these findings in reference to MAAs production and extraction from Fischerella sp. F5, for commercial-scale applications.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3109-3119"},"PeriodicalIF":2.4,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10084267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0