Molecular Biotechnology最新文献_第5页

Role of C/EBP Homologous Protein (CHOP) and Nupr1 Interaction in Endoplasmic Reticulum Stress-Induced Apoptosis of Lens Epithelial Cells. C/EBP同源蛋白（CHOP）和Nupr1在内质网应激诱导的晶状体上皮细胞凋亡中的作用

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-05-21 DOI: 10.1007/s12033-024-01148-z

Jinghua Li, Junyi Liu, Yongying Tang, Hong Zhang, Yuanping Zhang, Xu Zha, Xueying Zhao

{"title":"Role of C/EBP Homologous Protein (CHOP) and Nupr1 Interaction in Endoplasmic Reticulum Stress-Induced Apoptosis of Lens Epithelial Cells.","authors":"Jinghua Li, Junyi Liu, Yongying Tang, Hong Zhang, Yuanping Zhang, Xu Zha, Xueying Zhao","doi":"10.1007/s12033-024-01148-z","DOIUrl":"10.1007/s12033-024-01148-z","url":null,"abstract":"Our study mainly analyzed the mechanism of C/EBP homologous protein (CHOP) and its interacting protein Nupr1 on endoplasmic reticulum stress (ERS) induced lens epithelial cells (LEC) apoptosis. Cell proliferation was detected by CCK-8. Apoptosis was detected by flow cytometry and TUNEL. Nupr1 expression was detected by RT-qPCR. The expressions of CHOP, Nupr1, apoptosis-related protein, and ERS-related protein were detected by Western blot. DCFH-DA probe was used to detect cell ROS. The SOD, GSH-PX, and MDA contents were detected by the kit. Co-IP was used to detect the interaction between CHOP and Nupr1. The morphology of the lens was detected by HE staining. The result shows that Tunicamycin (TU) can induce endoplasmic reticulum stress and apoptosis in LEC in a concentration-dependent manner. TU induction leads to the occurrence of CHOP nuclear translocation. Overexpression of CHOP can further enhance the inhibitory effect of TU on LEC proliferation and the promotion of apoptosis, while knockdown of CHOP has the opposite effect. CHOP and Nupr1 are interacting proteins, and knockdown of Nupr1 or addition of Nupr1 inhibitor ZZW-115 can reverse the effects of TU and overexpression of CHOP, respectively. It has been observed in animal experiments that treatment with oe-CHOP can further aggravate the pathological lesions of the rat lens, while ZZW-115 can reverse the effect of oe-CHOP to a certain extent and improve the lesions of the rat lens. Overall, CHOP interacts with Nupr1 to regulate apoptosis caused by ERS and mediate cataract progression in rats, and this study provides a new potential therapeutic target for the treatment of cataract.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1628-1640"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Anatomical Nano Interbody Fusion Device for Elderly Patients Undergoing Anterior Discectomy and Interbody Fusion. 一种用于老年前路椎间盘切除术和椎间融合的新型解剖纳米椎间融合装置。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 DOI: 10.1007/s12033-025-01427-3

Shuang Tian, Pengliang Xu

{"title":"A New Anatomical Nano Interbody Fusion Device for Elderly Patients Undergoing Anterior Discectomy and Interbody Fusion.","authors":"Shuang Tian, Pengliang Xu","doi":"10.1007/s12033-025-01427-3","DOIUrl":"https://doi.org/10.1007/s12033-025-01427-3","url":null,"abstract":"To evaluate the perioperative outcomes of a novel anatomical nano-cage in elderly patients undergoing anterior discectomy and interbody fusion, with special attention to its outcomes on clinical symptoms, interbody fusion stability, and surgery-related complications.Methods: twenty-four cases of elderly surgical patients were divided into control group 1 (Cntrl 1), control group 2 (Cntrl 2), control group 3 (Cntrl 3), and the Experimental group (Exptl), 6 cases in each group. The polyetheretherketone interbody fusion cage was used in Cntrl 1 group, the titanium alloy interbody fusion cage was used in Cntrl 2 group, the spine minimally invasive interbody fusion cage was used in Cntrl 3 group, and the nano-hydroxyapatite/polyamide 66 (nHA/PA66) interbody fusion cage was used in Exptl group. All patients received anterior discectomy and interbody fusion, and received perioperative nursing intervention. The management of postoperative complications and patient observation indexes were recorded in detail.Results: The fusion of the fusion cage was better in the Exptl group than in the Cntrl group after surgery. At 2 weeks, 3 months, and 6 months after operation, the intervertebral space height of Exptl group was higher than that of Cntrl group (P < 0.05). Visual analogue scale (VAS) was lower in Exptl group than in Cntrl group (P < 0.05). At 2 weeks and 3 months after surgery, Japanese Orthopaedic Association (JOA) scores were higher in Exptl group than in Cntrl group (P < 0.05). Complications occurred more frequently in the Exptl group than in the Cntrl group.Conclusion: the novel anatomical nano-interbody fusion markedly improved the stability, the effective implementation of perioperative care reduced the incidence of complications, and the postoperative follow-up suggested that the successful application of interbody fusion cage had a positive impact on the overall rehabilitation of patients.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Trends of Gold Nanostructures for Point-of-Care Biosensor-Based Detection of COVID-19. 基于床旁生物传感器检测 COVID-19 的金纳米结构新动向

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-05-04 DOI: 10.1007/s12033-024-01157-y

Akhilesh Kumar Yadav, Nagaraj Basavegowda, Saba Shirin, Shiji Raju, Rajkumar Sekar, Prathap Somu, U T Uthappa, Gholamreza Abdi

{"title":"Emerging Trends of Gold Nanostructures for Point-of-Care Biosensor-Based Detection of COVID-19.","authors":"Akhilesh Kumar Yadav, Nagaraj Basavegowda, Saba Shirin, Shiji Raju, Rajkumar Sekar, Prathap Somu, U T Uthappa, Gholamreza Abdi","doi":"10.1007/s12033-024-01157-y","DOIUrl":"10.1007/s12033-024-01157-y","url":null,"abstract":"In 2019, a worldwide pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged. SARS-CoV-2 is the deadly microorganism responsible for coronavirus disease 2019 (COVID-19), which has caused millions of deaths and irreversible health problems worldwide. To restrict the spread of SARS-CoV-2, accurate detection of COVID-19 is essential for the identification and control of infected cases. Although recent detection technologies such as the real-time polymerase chain reaction delivers an accurate diagnosis of SARS-CoV-2, they require a long processing duration, expensive equipment, and highly skilled personnel. Therefore, a rapid diagnosis with accurate results is indispensable to offer effective disease suppression. Nanotechnology is the backbone of current science and technology developments including nanoparticles (NPs) that can biomimic the corona and develop deep interaction with its proteins because of their identical structures on the nanoscale. Various NPs have been extensively applied in numerous medical applications, including implants, biosensors, drug delivery, and bioimaging. Among them, point-of-care biosensors mediated with gold nanoparticles (GNPSs) have received great attention due to their accurate sensing characteristics, which are widely used in the detection of amino acids, enzymes, DNA, and RNA in samples. GNPS have reconstructed the biomedical application of biosensors because of its outstanding physicochemical characteristics. This review provides an overview of emerging trends in GNP-mediated point-of-care biosensor strategies for diagnosing various mutated forms of human coronaviruses that incorporate different transducers and biomarkers. The review also specifically highlights trends in gold nanobiosensors for coronavirus detection, ranging from the initial COVID-19 outbreak to its subsequent evolution into a pandemic.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1398-1422"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SIRT5 Inhibits Mitophagy and Inflammation of Hypoxia-Induced Pulmonary Hypertension by Regulating the Desuccinylation of PDK1. SIRT5通过调节PDK1去琥珀酰化抑制缺氧诱导的肺动脉高压的线粒体自噬和炎症。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 DOI: 10.1007/s12033-025-01430-8

Lin Guo, Kangkang Ji, Yi Yin

{"title":"SIRT5 Inhibits Mitophagy and Inflammation of Hypoxia-Induced Pulmonary Hypertension by Regulating the Desuccinylation of PDK1.","authors":"Lin Guo, Kangkang Ji, Yi Yin","doi":"10.1007/s12033-025-01430-8","DOIUrl":"https://doi.org/10.1007/s12033-025-01430-8","url":null,"abstract":"Hypoxia-induced pulmonary hypertension (HPH), a consequence of lung pathologies, is linked to changes in immune responses and inflammation. SIRT5 is recognized as the only enzyme capable of removing succinyl groups. The focus of this research was to explore the involvement of SIRT5 in HPH and to elucidate the associated mechanisms. Models simulating HPH were created in both living organisms and controlled laboratory settings under conditions of low oxygen. To investigate autophagy, transmission electron microscopy (TEM) was employed for ultrastructural analysis, while reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to measure the expression of autophagy-related genes. Cell viability was determined using the cell counting kit-8 (CCK-8) assay. The concentrations of inflammatory cytokines were quantified using ELISA, and flow cytometry was applied to evaluate reactive oxygen species (ROS) levels. To explore the interaction between PDK1 and SIRT5, co-immunoprecipitation (Co-IP) followed by Western blot analysis was conducted. Findings revealed that low oxygen conditions prompted mitophagy and elevated levels of both mRNA and proteins associated with this process in experiments conducted in organisms as well as in cellular models. Under conditions of low oxygen, the expression of SIRT5 was found to be reduced. Hypoxia enhanced cell viability, ROS level, angiogenesis-related protein levels, and inflammatory cytokine levels in pulmonary microvascular endothelial cells (PMVECs), effects that were reversed upon SIRT5 overexpression. Mechanistically, SIRT5 interacted with PDK1, desuccinylating PDK1 and thereby inhibiting mitophagy and inflammation associated with HPH. In conclusion, SIRT5 inhibited mitophagy and inflammation in HPH by regulating the desuccinylation of PDK1, potentially offering effective therapeutic strategies for treating HPH.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of an Antioxidant Aryl Polyene Pigment from Antarctic Bacterium Lysobacter sp. A03. 从南极细菌 Lysobacter sp.

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-03-28 DOI: 10.1007/s12033-024-01132-7

Maria Elisa Pailliè-Jiménez, Paolo Stincone, Jamile Queiroz Pereira, Patricio Román Santagapita, Eliseu Rodrigues, Adriano Brandelli

引用次数: 0

Identification of Prognostic Genes in Acute Myeloid Leukemia Microenvironment: A Bioinformatic and Experimental Analysis. 鉴定急性髓性白血病微环境中的预后基因：生物信息学和实验分析

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-05-07 DOI: 10.1007/s12033-024-01128-3

Ali Keshavarz, Amir Abbas Navidinia, Bentol Hoda Kuhestani Dehaghi, Vahid Amiri, Mohammad Hossein Mohammadi, Mehdi Allahbakhshian Farsani

{"title":"Identification of Prognostic Genes in Acute Myeloid Leukemia Microenvironment: A Bioinformatic and Experimental Analysis.","authors":"Ali Keshavarz, Amir Abbas Navidinia, Bentol Hoda Kuhestani Dehaghi, Vahid Amiri, Mohammad Hossein Mohammadi, Mehdi Allahbakhshian Farsani","doi":"10.1007/s12033-024-01128-3","DOIUrl":"10.1007/s12033-024-01128-3","url":null,"abstract":"Acute myeloid leukemia (AML) is a lethal hematologic malignancy with a variable prognosis that is highly dependent on the bone marrow microenvironment. Consequently, a better understanding of the AML microenvironment is crucial for early diagnosis, risk stratification, and personalized therapy. In recent years, the role of bioinformatics as a powerful tool in clarifying the complexities of cancer has become more prominent. Gene expression profile and clinical data of 173 AML patients were downloaded from the TCGA database, and the xCell algorithm was applied to calculate the microenvironment score (MS). Then, the correlation of MS with FAB classification, and CALGB cytogenetic risk category was investigated. Differentially expressed genes (DEGs) were identified, and the correlation analysis of DEGs with patient survival was done using univariate cox. The prognostic value of candidate prognostic DEGs was confirmed based on the GEO database. In the last step, real-time PCR was used to compare the expression of the top three prognostic genes between patients and the control group. During TCGA data analysis, 716 DEGs were identified, and survival analysis results showed that 152 DEGs had survival-related changes. In addition, the prognostic value of 31 candidate prognostic genes was confirmed by GEO data analysis. Finally, the expression analysis of FLVCR2, SMO, and CREB5 genes, the most related genes to patients' survival, was significantly different between patients and control groups. In summary, we identified key microenvironment-related genes that influence the survival of AML patients and may serve as prognostic and therapeutic targets.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1423-1432"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entrapment of Papain in Chitosan-Polyethylene Glycol Hybrid Nanohydrogels: Presenting a Model for Protein Delivery Systems. 壳聚糖-聚乙二醇杂化纳米水凝胶中木瓜蛋白酶的包封：为蛋白质输送系统树立典范

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-03-30 DOI: 10.1007/s12033-024-01129-2

Nasim Jafari, Saeed Najavand, Mohammad Pazhang, Amir Abbas Matin

{"title":"Entrapment of Papain in Chitosan-Polyethylene Glycol Hybrid Nanohydrogels: Presenting a Model for Protein Delivery Systems.","authors":"Nasim Jafari, Saeed Najavand, Mohammad Pazhang, Amir Abbas Matin","doi":"10.1007/s12033-024-01129-2","DOIUrl":"10.1007/s12033-024-01129-2","url":null,"abstract":"In this study, the process of manufacturing nanohydrogels containing papain and how to release it was investigated. Chitosan nanohydrogels and chitosan-polyethylene glycol hybrid nanohydrogels were used to entrapment of papain as a protein model. In order to evaluate and confirm different properties of nanohydrogels such as size, shape, the rate of swelling and flexibility, different methods was used. The maximum amount of papain entrapment was observed in 0.75% concentration of chitosan and 1% concentration of sodium Tripolyphosphate (TPP) as linker. The results of scanning electron microscope (SEM) and X-ray diffraction (XRD) patterns showed that nanohydrogels containing papain on a nano scale are very porous and swollen. Differential scanning calorimetry (DSC) thermograms analysis showed that nanohydrogels have relatively good water absorption capacity. Also, by adding polyethylene glycol to chitosan, the melting temperature of hybrid nanohydrogels decreased and this can be a reason for the formation of flexible structures in these nanohydrogels. In chitosan nanohydrogels, the highest release rate of papain was observed at pH lower than 7 and high temperatures, but by adding polyethylene glycol to the chitosan, in addition to increasing papain release, a proper and continuous release of papain was observed at temperature and pH close to physiological conditions, especially at low ratios of polyethylene glycol. According to the present results, hybrid nanohydrogels can have a good potential in protein delivery systems in terms of structure and release.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1433-1445"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140330021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Ferroptosis-Inflammation Related Hub Genes and the Disease Subtypes in Idiopathic Pulmonary Fibrosis via System Biology Approaches. 通过系统生物学方法鉴定特发性肺纤维化的铁蛋白沉积-炎症相关枢纽基因和疾病亚型。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-04-01 Epub Date: 2024-05-11 DOI: 10.1007/s12033-024-01158-x

Chongyang Niu, Xiaoyu Meng, Tan Wang

{"title":"Identification of Ferroptosis-Inflammation Related Hub Genes and the Disease Subtypes in Idiopathic Pulmonary Fibrosis via System Biology Approaches.","authors":"Chongyang Niu, Xiaoyu Meng, Tan Wang","doi":"10.1007/s12033-024-01158-x","DOIUrl":"10.1007/s12033-024-01158-x","url":null,"abstract":"We aim to screen and analyze the ferroptosis inflammation-related hub genes associated with idiopathic pulmonary fibrosis (IPF). The GSE52463 and GSE110147 datasets were obtained from the GEO database and merged. The DEGs were selected by differential analysis and intersected with inflammation-related genes and ferroptosis-related genes to acquire the ferroptosis-related differentially expressed genes (FRDEGs). GO, KEGG, GSEA, and GSVA were performed to investigate the features of FRDEGs. The key module genes were selected by WGCNA and employed to generate the PPI network using Cytoscape. Subsequently, the hub genes were identified using cytoHubba and validated by ROC curves generated by survivalROC. Finally, the correlations of hub genes were analyzed through Spearman and the subtypes of IPF were constructed using ConsensusClusterPlus. A total of 1814 DEGs were screened out and 18 FRDEGs were acquired from the intersection of DEGs, ferroptosis-related genes, and inflammation-related genes. GO and KEGG analysis revealed that FRDEGs were primarily involved in bacterial-origin molecular, response infectious disease, and iron ion transport. GSEA results suggested a predominant association with autoimmune diseases and GSVA identified ten different pathways between PF and control. Through WGCNA, three highly correlated modules were identified and ten key module genes were obtained by intersecting genes in the three modules with FRDEGs. Finally, employing three algorithms within the cytoHubba led to the identification of eight hub genes: CCND1, TP53, STAT3, CTNNB1 CDH1, ESR1, HSP90AA1, and EP300. Eventually, two distinct subtypes of IPF were identified. The present research successfully identified the hub genes associated with ferroptosis and inflammation and their biological effects on IPF. Furthermore, two disease subtypes of IPF were constructed.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"1720-1733"},"PeriodicalIF":2.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140907647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Motor Proteins to Oncogenic Factors: The Evolving Role of Kinesin Superfamily Proteins in Breast Cancer Development. 从运动蛋白到致癌因子：运动蛋白超家族蛋白在乳腺癌发展中的进化作用。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-03-27 DOI: 10.1007/s12033-025-01428-2

Mohamed J Saadh, Hanan Hassan Ahmed, Radhwan Abdul Kareem, Muktesh Chandra, Mekha Monsi, Chakshu Walia, G V Siva Prasad, Waam Mohammed Taher, Mariem Alwan, Mahmood Jasem Jawad, Atheer Khdyair Hamad

{"title":"From Motor Proteins to Oncogenic Factors: The Evolving Role of Kinesin Superfamily Proteins in Breast Cancer Development.","authors":"Mohamed J Saadh, Hanan Hassan Ahmed, Radhwan Abdul Kareem, Muktesh Chandra, Mekha Monsi, Chakshu Walia, G V Siva Prasad, Waam Mohammed Taher, Mariem Alwan, Mahmood Jasem Jawad, Atheer Khdyair Hamad","doi":"10.1007/s12033-025-01428-2","DOIUrl":"https://doi.org/10.1007/s12033-025-01428-2","url":null,"abstract":"The kinesin family of proteins (KIFs), known for their role as motor proteins, is integral to transporting cargo within cells along microtubule tracks, which is crucial for processes, such as cell division, differentiation, and intracellular communication. Increasing evidence shows that specific KIFs are overexpressed in breast cancer, a change linked to higher tumor aggression and poorer outcomes in patients. KIFs contribute to the cancerous characteristics of breast tumor cells through several mechanisms, including disruptions in spindle assembly during cell division, altered cell motility, and accelerated proliferation. This review summarizes current insights into KIFs' functions in breast cancer pathology and assesses their viability as therapeutic targets. By unraveling the complex involvement of KIFs, the article aims to open pathways for new therapeutic approaches in breast cancer and to promote further study into the cellular pathways that these proteins regulate.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenesis in Drug Discovery: Enhancing Target Identification and Validation. 药物发现中的转基因：增强靶标识别和验证。

IF 2.4 4区生物学

Molecular Biotechnology Pub Date : 2025-03-27 DOI: 10.1007/s12033-025-01426-4

Rumela Mete, Sourav Das, Arindam Saha, Sukanta Roy, Smritilekha Mondal, Anirbandeep Bose, Biswajit Basu, Gehan M Elossaily, Bhupendra Prajapati

{"title":"Transgenesis in Drug Discovery: Enhancing Target Identification and Validation.","authors":"Rumela Mete, Sourav Das, Arindam Saha, Sukanta Roy, Smritilekha Mondal, Anirbandeep Bose, Biswajit Basu, Gehan M Elossaily, Bhupendra Prajapati","doi":"10.1007/s12033-025-01426-4","DOIUrl":"https://doi.org/10.1007/s12033-025-01426-4","url":null,"abstract":"Transgenesis, the introduction of foreign genetic material into the genome of an organism, has become a crucial and transformative technique in the realm of drug discovery. This review article provides a comprehensive overview of the integral role that transgenesis plays in the drug discovery process, with a specific focus on target identification and target validation. By examining the recent advancements and innovative approaches, this article aims to shed light on the importance of transgenesis in accelerating drug development. In the context of target identification, transgenesis has allowed for the creation of relevant disease models, enabling researchers to study the genetic and molecular basis of various disorders. The use of transgenic animals, such as mice and zebrafish, has facilitated the identification of potential drug targets by mimicking specific human disease conditions. This review also discusses emerging technologies, such as CRISPR-Cas9 and other genome editing tools, which have revolutionized the field of transgenesis. These technologies have enhanced the precision and efficiency of genetic manipulations in transgenic animals, making the creation of disease-relevant models more accessible and cost-effective. Moreover, integration of omics technologies, such as genomics, transcriptomics, proteomics, and metabolomics, has provided a holistic view of the molecular changes in transgenic models, further aiding in target identification and validation. This review emphasizes the importance of transgenesis in target identification and validation and underscores its vital role in shaping the future of drug discovery.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0