Molecular Biotechnology最新文献_第5页

Deciphering Microalgal Diversity of Peculiar Lentic Ecosystem in Chennai, South India: A Way Towards Sustainability. 解读印度南部钦奈特殊生态系统的微藻多样性：一条通往可持续发展的道路。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-07-27 DOI: 10.1007/s12033-023-00825-9

Keerthivarman G Subramanian, Manikandavelu Dhanushkodi, Aruna Satyapriyan, Muralidharan Nagarajan, Govarthanan Muthusamy

{"title":"Deciphering Microalgal Diversity of Peculiar Lentic Ecosystem in Chennai, South India: A Way Towards Sustainability.","authors":"Keerthivarman G Subramanian, Manikandavelu Dhanushkodi, Aruna Satyapriyan, Muralidharan Nagarajan, Govarthanan Muthusamy","doi":"10.1007/s12033-023-00825-9","DOIUrl":"10.1007/s12033-023-00825-9","url":null,"abstract":"Microalgae are quickly gaining attention among the researchers in various aspects such as biofuel applications, biogas, biomass production, waste water treatment, carbon fixation, animal feed ingredients, pigment production, and pharmaceutical products. One of the approaches to choose microalgae for biotechnological applications is to investigate their diversity and abundance in all possible wet environments. Samples were collected from three sampling sites for the period of 1 year (October 2021-September 2022) in Vadapalani temple tank at Chennai. Physicochemical parameters in current investigation were estimated according to APHA, 2017. Qualitative and quantitative analyses of phytoplankton were done throughout the study period. One-way ANOVA (Analyses of Variance) and Karl Pearson's correlation coefficient were estimated using SPSS (V.26.0). A total of 11 diversity indices were estimated using PAST (V 4.0). A total of 52 algal species were identified, prevailed over by Chlorophyceae (15 species), followed by Zygnematophyceae, Bacillariophyceae, Cyanophyceae, and Trebouxiophyceae. Chlorophyceae quantitatively structured the major category. The maximum and minimum values of density were observed during the season of summer (287 Cells/L) and monsoon (80 Cells/L), respectively. Chlorophyceae showed dominance with a density of 168 cells/L. The maximum and minimum densities of Chlorophyceae were recorded in the summer season (55 cells/L) and monsoon season (24 cells/L), respectively. Shannon's index (H') attained its zenith in February and April month of 2022 (3.60). This study further ignites the researchers to phycoprospect various temple water to address the nature of microalgae occurrence and for biotechnological purposes.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3409-3420"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9886121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED ARTICLE: Preparation and Characterization of Magnetite-Polyvinyl Alcohol Hybrid Nanoparticles (As-PVA-MNPs) Using Acanthophora spicifera Marine Algae Extract for Enhanced Antimicrobial Activity Against Pathogenic Microorganisms. 利用刺五加海藻提取物制备和表征磁性-聚乙烯醇杂化纳米颗粒（作为PVA-MNPs），以增强对病原微生物的抗菌活性。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-10-31 DOI: 10.1007/s12033-023-00903-y

D S Ranjith Santhosh Kumar, S Hari Puthiran, Gayathri Devi Selvaraju, Paul A Matthew, P Senthilkumar, Sowmya Kuppusamy, Ravishankar Ram Mani, Ashraf Atef Hatamleh, Munirah Abdullah Ai-Dosary, Soon Woong Chang, Balasubramani Ravindran

引用次数: 0

Advances in Molecular Tools for Fungal Pathogen Detection and Identification in Common Bean: A Systematic Review. 大豆真菌病原检测与鉴定分子工具研究进展

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2026-03-31 DOI: 10.1007/s12033-026-01572-3

Khulasande Siyabonga Ngcobo, Sandiswa Figlan, Molemi Rauwane

{"title":"Advances in Molecular Tools for Fungal Pathogen Detection and Identification in Common Bean: A Systematic Review.","authors":"Khulasande Siyabonga Ngcobo, Sandiswa Figlan, Molemi Rauwane","doi":"10.1007/s12033-026-01572-3","DOIUrl":"10.1007/s12033-026-01572-3","url":null,"abstract":"Common bean (Phaseolus vulgaris L.) is crucial for global food security but is highly vulnerable to fungal pathogens that impact yield and quality. Molecular tools have transformed pathogen detection, enabling accuracy, sensitivity, and rapid diagnostics. This review, following PRISMA 2020 guidelines, synthesizes recent advancements in molecular methods, including PCR, qPCR, isothermal amplification, next-generation sequencing (NGS), and bioinformatics pipelines for the detection and identification of fungal pathogens in common bean. While PCR-based methods dominate, NGS and metabarcoding offer deeper insights into fungal communities. Challenges remain, such as primer specificity, high costs, and the need for robust databases. Additionally, the growing use of NGS highlights the demand for trained bioinformaticians to manage large datasets. Addressing these challenges through training programs and interdisciplinary collaboration is crucial for improving pathogen detection. This review underscores the potential of emerging molecular technologies to enhance fungal disease management and calls for scalable, affordable solutions suited to diverse agricultural systems.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3305-3313"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Downregulation of AQP9 Ameliorates NLRP3 Inflammasome-Dependent Inflammation and Pyroptosis in Crohn's Disease by Inhibiting the p38 MAPK Signaling Pathway. 纠正：下调AQP9通过抑制p38 MAPK信号通路改善克罗恩病NLRP3炎症小体依赖性炎症和焦亡。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 DOI: 10.1007/s12033-026-01568-z

Qin-Qin Zhu, Yin Zhang, Lu Cui, Liang Ma, Ke-Wen Sun

引用次数: 0

RETRACTED ARTICLE: Unleashing the Potential of Benincasa hispida Peel Extract: Synthesizing Selenium Nanoparticles with Remarkable Antibacterial and Anticancer Properties. 发挥蛇床子皮提取物的潜力：合成具有显著抗菌抗癌性能的硒纳米粒子。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-09-26 DOI: 10.1007/s12033-023-00884-y

Salman Khan, Zeshan Rafi, Pooja Mishra, Lamya Ahmed Al-Keridis, Alvina Farooqui, Shazia Mansoor, Nawaf Alshammari, Fatimah A Al-Saeed, Samra Siddiqui, Mohd Saeed

引用次数: 0

Microbial Diversity and Functional Potential of Keem: A Traditional Starter Culture for Alcoholic Beverage-Application of Next-Generation Amplicon and Shotgun Metagenome Sequences. 传统酒精饮料发酵剂Keem的微生物多样性和功能潜力——新一代扩增子和散弹枪宏基因组序列的应用。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-08-11 DOI: 10.1007/s12033-023-00839-3

Shikha Tomar, Debasis Mitra, Gaurav Kumar, Piyush Kashyap, Minaxi Sharma, Shiv Kumar, Kandi Sridhar, Kumud Pant

{"title":"Microbial Diversity and Functional Potential of Keem: A Traditional Starter Culture for Alcoholic Beverage-Application of Next-Generation Amplicon and Shotgun Metagenome Sequences.","authors":"Shikha Tomar, Debasis Mitra, Gaurav Kumar, Piyush Kashyap, Minaxi Sharma, Shiv Kumar, Kandi Sridhar, Kumud Pant","doi":"10.1007/s12033-023-00839-3","DOIUrl":"10.1007/s12033-023-00839-3","url":null,"abstract":"\"Pakhoi\" is an ethnic drink of the Tons valley, Uttarakhand, India produced by fermenting jaggery and barley with the help of a starter culture called \"keem\". In the present study, we investigated the microbial diversity and associated functional potential of \"keem\" using shotgun metagenome sequencing and amplicon sequencing. We also compared the taxonomic data obtained using these two sequencing techniques. The results showed that shotgun sequencing revealed a higher resolution of taxonomic profiling as compared to the amplicon sequencing. Furthermore, it was found that the genera detected by shotgun sequencing were valuable for facilitating the fermentation process. Additionally, to understand the functional profiling of the genera, different databases were used for annotation, resulting in a total of 13 metabolic pathways. The five most abundant KEGG functions were genetic information processing, metabolism, translation, cofactor and vitamin metabolism and xenobiotic degradation. In contrast, the top five COG were in order of highest frequency sequences belonging to transcription, followed by general function prediction, carbohydrate transport metabolism, amino acid transport and metabolism and translation and biogenesis. Gene ontology revealed many pathways, biochemical processes and molecular functions associated with the organisms forming the starter culture. Overall, the present study can help to understand the microbial diversity and its role in fermentation of traditional alcoholic beverages using \"Keem\".","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3455-3468"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9975213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of AQP9 Ameliorates NLRP3 Inflammasome-Dependent Inflammation and Pyroptosis in Crohn's Disease by Inhibiting the p38 MAPK Signaling Pathway. 下调AQP9通过抑制p38 MAPK信号通路改善克罗恩病NLRP3炎症小体依赖性炎症和焦亡

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2025-02-10 DOI: 10.1007/s12033-025-01382-z

Qin-Qin Zhu, Yin Zhang, Lu Cui, Liang Ma, Ke-Wen Sun

{"title":"Downregulation of AQP9 Ameliorates NLRP3 Inflammasome-Dependent Inflammation and Pyroptosis in Crohn's Disease by Inhibiting the p38 MAPK Signaling Pathway.","authors":"Qin-Qin Zhu, Yin Zhang, Lu Cui, Liang Ma, Ke-Wen Sun","doi":"10.1007/s12033-025-01382-z","DOIUrl":"10.1007/s12033-025-01382-z","url":null,"abstract":"Crohn's disease (CD), a complex gastrointestinal disorder, can be attributed to a combination of genetic factors, immune system dysfunction, and environmental triggers. Aquaporin 9 (AQP9) has been implicated in immunoregulation and inflammation in various conditions, yet its function in CD remains unclear. Herein, we investigated the contribution of AQP9 to CD pathogenesis and its impact on inflammation and pyroptosis. Bioinformatic analysis showed a significant increase in AQP9 expression (above 2.5-fold change) in CD patients compared to controls. In vitro experiments using human colonic epithelial cells (HT-29) demonstrated that AQP9 inhibition attenuated lipopolysaccharide (LPS)-induced cell damage, inflammatory cytokine secretion, and pyroptosis. Mechanistically, AQP9 silencing suppressed NLRP3 inflammasome activation, suggesting a role in regulating pyroptosis. AQP9 silencing inhibited p38 MAPK phosphorylation, indicating a direct involvement in modulating this inflammatory pathway. Furthermore, our findings indicate that AQP9 exacerbates inflammation and pyroptosis via activating the p38 MAPK signaling pathway, known to contribute to CD pathogenesis. In vivo studies using a murine model of CD-like colitis revealed that AQP9 inhibition led to about 45% reduction in colitis severity scores and about 30% decrease in the production of inflammatory cytokine by inactivating NLRP3 inflammasome and the p38 MAPK signaling. To sum up, our study highlights the involvement of AQP9 in CD pathogenesis through modulation of inflammation and pyroptosis via the NLRP3 inflammasome and p38 MAPK signaling pathway. Targeting AQP9 may offer a promising therapeutic approach for CD by suppressing inflammatory responses and preventing tissue damage.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3383-3396"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA Crosstalk in Metabolic Disorders: The Dual Role of miR-122 and miR-34a in NAFLD and Type 2 Diabetes. 代谢紊乱中的MicroRNA串扰：miR-122和miR-34a在NAFLD和2型糖尿病中的双重作用

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2026-04-27 DOI: 10.1007/s12033-026-01576-z

Sumita Thakur, Anjana Munshi, Prabhsimran Kaur

{"title":"MicroRNA Crosstalk in Metabolic Disorders: The Dual Role of miR-122 and miR-34a in NAFLD and Type 2 Diabetes.","authors":"Sumita Thakur, Anjana Munshi, Prabhsimran Kaur","doi":"10.1007/s12033-026-01576-z","DOIUrl":"10.1007/s12033-026-01576-z","url":null,"abstract":"NAFLD and T2DM are metabolic diseases with overlapping pathogenic mechanisms, including insulin resistance, lipid imbalance, and chronic inflammation. miRNAs have emerged as reliable biomarkers for NAFLD and T2DM. The expression levels of miRNAs hold the potential to contribute to bridging the gap between metabolic diseases. miR-122 and miR-34a are significant regulators of metabolic pathways among various biomarkers. miR-122, a liver-enriched microRNA involved in hepatic lipid metabolism, inflammation, and insulin signaling, whereas miR-34a is closely linked to β-cell dysfunction and hepatic steatosis through SIRT1 suppression. Growing echelons of these miRNAs in the bloodstream of patients with T2DM and NAFLD show their potential use as prognostic and diagnostic indicators. These enable a non-invasive early disease monitoring and detection alternative. miR-122 and miR-34a are involved in disease progression by influencing key metabolic processes such as lipid metabolism, pro-inflammatory cytokine regulation, the SIRT1-SREBP1c pathway, and AMPK phosphorylation. Their changed expression levels in various metabolic conditions contribute to advancing pathophysiological processes, explaining their clinical value as therapeutic targets. This review investigates the molecular functions of miR-122 and miR-34a in NAFLD and T2DM, emphasizing their effect on various genes and interrelated pathways, showing their diagnostic potential and therapeutic implications. Knowing their regulatory roles might lead the way for miRNA-based precision medicine techniques that improve early diagnosis, risk assessment, and treatment strategies for metabolic illnesses. Future research should focus on their therapeutic use and make targeted interventions to change their expression levels for disease management.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3323-3343"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Diabetic Neuropathy on Reparative Ability and Immune Response System. 糖尿病性神经病变对神经修复能力和免疫反应系统的影响。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-07-31 DOI: 10.1007/s12033-023-00813-z

Emina Karahmet Sher, Besim Prnjavorac, Esma Karahmet Farhat, Benjamin Palić, Sabah Ansar, Farooq Sher

{"title":"Effect of Diabetic Neuropathy on Reparative Ability and Immune Response System.","authors":"Emina Karahmet Sher, Besim Prnjavorac, Esma Karahmet Farhat, Benjamin Palić, Sabah Ansar, Farooq Sher","doi":"10.1007/s12033-023-00813-z","DOIUrl":"10.1007/s12033-023-00813-z","url":null,"abstract":"The effects of diabetes can be divided into short, medium and long term and various human organ systems can be effected. The present study aimed to determine how much the duration of diabetes mellitus (DM) affect the reparative ability of the body, immune response and the development of DM complications. Interleukin 1-β (IL-1β) and Interleukin 6 (IL-6) were monitored as specific indicators of inflammatory reaction and C-reactive protein (CRP), leukocyte count (WBC) and sedimentation rate (ESR) as general markers of inflammatory reaction. Tumour necrosis factor α (TNF-α) and transforming growth factor β1 (TGF-β1) were observed as indicators of reparative ability and polyneuropathy. All interleukins were determined by ELISA and evaluated spectrophotometrically. Michigan Neuropathy Screening Instrument (MNSI) is performed for neuropathy examination. Patients with diabetes mellitus were divided into 3 groups, according to duration of diabetes mellitus. IL-6 levels correlated with clinical stage of diabetic polyneuropathy at p = 0.025 R = 0.402; with CRP at p = 0.0001, R = 0.784 as well as correlation of CRP and MNSI score (R = 0.500, p = 0.034) in a group of patients with DM lasting up to 10 years. The reparative ability of the body is reduced by physiological age and ages of DM duration. The immune response is weakened in DM additionally. The dual activity of cytokines IL-6 and TGF-β1 is present in long-duration Diabetes Mellitus.","PeriodicalId":18865,"journal":{"name":"Molecular Biotechnology","volume":" ","pages":"3421-3434"},"PeriodicalIF":3.2,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13518443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9889314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED ARTICLE: Spirulina Cultivation Under Different light-emitting Diodes for Boosting Biomass and Protein Production. 在不同发光二极管下培养螺旋藻以提高生物量和蛋白质产量。

IF 3.2 4区生物学

Molecular Biotechnology Pub Date : 2026-08-01 Epub Date: 2023-08-03 DOI: 10.1007/s12033-023-00842-8

Obaid Bhat, Yuwalee Unpaprom, Rameshprabu Ramaraj

引用次数: 0