DcMADS3, a MADS-Box Transcription Factor, Highly Expressed at the Middle Flower Bud Stage and Involved in Petalization Development in Carrot.

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-07-31 DOI:10.1007/s12033-025-01489-3

Shan-Shan Tan, Shan-Shan Liu, Jing Ma, Yi Liang, Ao-Qi Duan, Yuan-Jie Deng, Ya-Hui Wang, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong

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引用次数: 0

Abstract

Carrot (Daucus carota), an Apiaceae family crop, possesses the typical compound umbellate inflorescence with small flowers. The mechanism of carrot floral development is still unknown. APETALA3 (AP3) is a MADS-box transcription factor that primarily participates in stamen and petal development. In this study, DcMADS3 belonging to the APETALA3 group was cloned, which can form a heterodimer with DcMADS2 belonging PI (PISTILLATA) group. Expression analysis revealed DcMADS3 was higher in 'Kurodagosun' (normal flower, KRD) than 'Deep Purple' ('petaloid' CMS phenotype, DPP) at the middle flower bud stage. Its expression was greater in DPP petals (normal petal and petaloid petal) than in KRD petals, suggesting its potential role in regulating petalization development in carrot. Transgenic Arabidopsis overexpressing DcMADS3 resulted in altered petal size, increased petal number, and altered flower symmetry. Furthermore, yeast one-hybrid (Y1H) library screening identified DcXTH-like (DCAR_009699) as a potential factor binding to the DcMADS3 promoter. These results provided a theoretical foundation for further investigation of carrot floral organ development mechanism.

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胡萝卜MADS-Box转录因子DcMADS3在花芽中期高表达并参与花瓣化发育。

胡萝卜（Daucus carota）是一种蜂科作物，具有典型的复伞形花序和小花。胡萝卜花发育的机制尚不清楚。aptala3 （AP3）是一种主要参与雄蕊和花瓣发育的MADS-box转录因子。本研究克隆了属于APETALA3群的DcMADS3，与属于PI （PISTILLATA）群的DcMADS2形成异源二聚体。表达分析显示，在花蕾中期，DcMADS3在‘Kurodagosun’（正常花，KRD）中的表达量高于‘Deep Purple’（‘petaloid’CMS表型，DPP）。其在DPP花瓣（正常花瓣和类花瓣）中的表达量高于KRD花瓣，提示其在胡萝卜花瓣发育中的潜在调控作用。过表达DcMADS3的转基因拟南芥会改变花瓣大小，增加花瓣数量，改变花的对称性。此外，酵母单杂交（Y1H）文库筛选发现dcxth样（DCAR_009699）是与DcMADS3启动子结合的潜在因子。这些结果为进一步研究胡萝卜花器官发育机制提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.