Investigating the Role of OsHDT701 and Other Blast-Associated Negative Regulatory Genes in Indica Rice Cultivar Ranjit Using Combined Wet Lab and Computational Approaches.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2024-10-30 DOI:10.1007/s12033-024-01310-7

Yogita N Sarki, Hidam Bishworjit Singh, Ajay Kumar Keot, Riwandahun Marwein, Dhanawantari L Singha, Budheswar Dehury, Channakeshavaiah Chikkaputtaiah

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引用次数: 0

Abstract

Rice blast, caused by Magnaporthe oryzae, severely impacts global rice production. Understanding the role of the host's blast negative regulatory genes is crucial for combating this disease. We studied the expression of seven rice blast negative regulatory genes (previously characterized in ssp. japonica) in susceptible (Ranjit and Mahsuri) and tolerant/resistant (Shahsarang 1 and IR64) indica rice cultivars during M. oryzae infection. The selected genes showed differential expression patterns, with OsHDT701 (histone H4 deacetylase) downregulated in tolerant/resistant and upregulated in susceptible cultivars. Promoter analysis of OsHDT701 in cv. Ranjit revealed biotic stress-responsive and M. oryzae effector binding motifs (MoHTR2 and MoSPAB1). Protein-DNA docking showed an interaction for both the effectors and promoter sequences. Furthermore, a strong interaction between the OsERF922 transcription factor (a negative regulator of rice blast) and GCC box at the promoter suggests OsERF922-mediated regulation of OsHDT701. Next, a comparative analysis of ssp. japonica (Nipponbare) OsHDT701 CDS to 11 indica rice cultivars, including Ranjit, detected an SNP (T-A) at position 591. Moreover, the structure modeling and protein-protein interaction reveal that OsHDT701 may form a complex with other histone deacetylases to regulate defense-related gene expression. Taken together, our study unveils new possibilities for OsHDT701 regulation mechanisms during M. oryzae infection.

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采用湿法实验室和计算相结合的方法研究籼稻栽培品种 Ranjit 中 OsHDT701 和其他与爆炸相关的负调控基因的作用。

由 Magnaporthe oryzae 引起的稻瘟病严重影响全球水稻生产。了解宿主稻瘟病负调控基因的作用对于防治这种疾病至关重要。我们研究了易感（Ranjit 和 Mahsuri）和耐/抗（Shahsarang 1 和 IR64）籼稻栽培品种在稻瘟病菌感染期间七个稻瘟病负调控基因的表达情况（以前在粳稻中表征过）。所选基因表现出不同的表达模式，其中 OsHDT701（组蛋白 H4 去乙酰化酶）在耐受/抗性栽培品种中下调，而在易感栽培品种中上调。在 Ranjit 作物中，OsHDT701 的启动子分析表明，生物胁迫对其有影响。Ranjit 中的启动子分析表明，OsHDT701 具有生物胁迫响应性和 M. oryzae效应结合基团（MoHTR2 和 MoSPAB1）。蛋白质与 DNA 的对接显示，效应因子与启动子序列之间存在相互作用。此外，OsERF922 转录因子（稻瘟病的负调控因子）与启动子上的 GCC box 之间的强相互作用表明 OsERF922 介导了对 OsHDT701 的调控。接着，对粳稻（Nipponbare）OsHDT701 CDS 和包括 Ranjit 在内的 11 个籼稻栽培品种进行了比较分析，在 591 位检测到一个 SNP（T-A）。此外，结构建模和蛋白相互作用揭示 OsHDT701 可能与其他组蛋白去乙酰化酶形成复合物，以调控防御相关基因的表达。综上所述，我们的研究为 OsHDT701 在 M. oryzae 感染过程中的调控机制揭示了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.