Overcoming Multidrug Resistance in E. coli and Salmonella Isolates from Nile Tilapia: Synergistic Effects of Novel Antibiotic Combinations.

IF 2.5 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2025-06-29 DOI:10.1007/s12033-025-01462-0

Laiba Shafique, Muhammad Farhan Khan, Shakeela Parveen, Youhou Xu, Peng Zhu

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引用次数: 0

Abstract

Escherichia coli and Salmonella are significant foodborne zoonotic pathogens, causing serious human illness. The rising global prevalence of antimicrobial resistance (AMR) in these species exacerbates their public health risk, complicating the treatment of bacterial infection. This study investigates its prevalence, resistant genes, and treatment strategy against antibiotic-resistant bacteria, focusing on E. coli and Salmonella isolates from Nile tilapia. Prevalence of E. coli and Salmonella was found to be 32 and 22% respectively. Antibiotic susceptibility testing revealed resistance to five antibiotics in E. coli and four in Salmonella. Physiochemical properties of antibiotic resistance genes (ABRGs) indicated that the TetB gene has the highest aliphatic index in both bacteria, suggesting greater stability. All Bla proteins were hydrophobic as indicated by negative GRAVY values, which may contribute to antibiotic efflux or modification of antibiotic targets. Motif analysis identified functional domains, and cellular localization prediction showed that TetA and TetB genes are primarily expressed in the cell membrane. To combat this resistance, a checkerboard method was used to explore novel antibiotic combinations. For E. coli, one synergistic and two additive combinations were identified, while for Salmonella, two synergistic and one additive combination were effective. These results highlight the importance of regularly evaluating antibiotic combinations to combat resistance and preserve antibiotic efficacy.

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克服尼罗罗非鱼分离的大肠杆菌和沙门氏菌的多重耐药：新型抗生素组合的协同效应。

大肠杆菌和沙门氏菌是重要的食源性人畜共患病原体，可引起严重的人类疾病。这些物种抗菌素耐药性（AMR）的全球流行率不断上升，加剧了它们的公共卫生风险，使细菌感染的治疗复杂化。本研究调查了尼罗罗非鱼的流行情况、耐药基因和抗抗生素耐药细菌的治疗策略，重点研究了从尼罗罗非鱼中分离出的大肠杆菌和沙门氏菌。大肠杆菌和沙门氏菌的感染率分别为32%和22%。抗生素敏感性测试显示大肠杆菌对5种抗生素有耐药性，沙门氏菌对4种抗生素有耐药性。抗生素耐药基因（ABRGs）的理化性质表明，TetB基因在两种细菌中具有最高的脂肪族指数，表明其具有更高的稳定性。所有的Bla蛋白都是疏水的，肉汁值为负，这可能有助于抗生素外排或抗生素靶点的修饰。基序分析确定了功能域，细胞定位预测表明TetA和TetB基因主要在细胞膜上表达。为了对抗这种耐药性，采用棋盘法探索新的抗生素组合。对大肠杆菌有一种增效组合和两种添加剂组合，对沙门氏菌有两种增效组合和一种添加剂组合。这些结果突出了定期评估抗生素组合以对抗耐药性和保持抗生素疗效的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.