Molecular plant pathology最新文献_第4页

The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants. csn-miR393a 对辅助素受体基因 CsAFB2 的调控赋予了茶树对球孢子菌的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.13499

Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li

{"title":"The regulation of auxin receptor gene CsAFB2 by csn-miR393a confers resistance against Colletotrichum gloeosporioides in tea plants.","authors":"Anburaj Jeyaraj, Shujing Liu, Rui Han, Yuxin Zhao, Tamilselvi Elango, Yuhua Wang, Xuan Chen, Jing Zhuang, Xinghui Li","doi":"10.1111/mpp.13499","DOIUrl":"10.1111/mpp.13499","url":null,"abstract":"Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression in response to disease resistance, defence responses and plant immunity. However, the regulatory mechanisms of miRNAs in responses to Colletotrichum gloeosporioides remain unknown in tea plants. Our study revealed that csn-miR393a targets auxin receptor gene CsAFB2 during resistance to C. gloeosporioides in tea plants by comparing the resistant cultivar Zhongcha108 to the susceptible cultivar Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated that csn-miR393a suppresses the expression of CsAFB2, and csn-miR393a target mimic blocks the function of csn-miR393a, leading to increase in the expression of CsAFB2. Repression of transcripts in tea leaves by antisense oligonucleotides demonstrated that csn-miR393a negatively affects the tea plant defence by regulating reactive oxygen species homoeostasis, PR gene expression and catechin accumulation. To further validate the regulatory mechanisms of csn-miR393a, we developed transgenic tea plants overexpressing CsAFB2, resulting in enhanced resistance responses against C. gloeosporioides. Additionally, transgenic N. benthamiana lines overexpressing a csn-miR393a target mimic provided further evidence that csn-miR393a negatively regulates the tea plant defence response against C. gloeosporioides by suppressing CsAFB2. Therefore, manipulating csn-miR393a or its target gene, CsAFB2, has the potential to strengthen the tea plant's resistance against tea anthracnose.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e13499"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950636/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Necrotrophic Phytopathogen-Derived GPI-Anchored Protein Functions as an Elicitor to Activate Plant Immunity and Enhance Resistance. 一种坏死性植物病原体衍生的gpi锚定蛋白作为启动子激活植物免疫并增强抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70072

Guangxing Miao, Jiatao Xie, Yanping Fu, Bo Li, Tao Chen, Yang Lin, Xiao Yu, Tom Hsiang, Daohong Jiang, Jiasen Cheng

引用次数: 0

Transcription Factor SsNdt80b Maintains Optimal Expression of SsSNF1 to Modulate Growth and Pathogenicity in Sclerotinia sclerotiorum. 转录因子SsNdt80b维持SsSNF1的最佳表达以调节菌核菌的生长和致病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70088

Wenli Jiao, Tianyi Lei, Qingyu Duan, Jingyuan Wang, Yushan Yang, Guang Li, Rongbao Zhang, Hongyu Pan, Yanhua Zhang

{"title":"Transcription Factor SsNdt80b Maintains Optimal Expression of SsSNF1 to Modulate Growth and Pathogenicity in Sclerotinia sclerotiorum.","authors":"Wenli Jiao, Tianyi Lei, Qingyu Duan, Jingyuan Wang, Yushan Yang, Guang Li, Rongbao Zhang, Hongyu Pan, Yanhua Zhang","doi":"10.1111/mpp.70088","DOIUrl":"https://doi.org/10.1111/mpp.70088","url":null,"abstract":"Microorganisms use versatile strategies to facilitate the colonisation of hosts, through remodelling transcription and metabolism to accommodate growth under harsh and hostile environments. Sclerotinia sclerotiorum is a typical necrotrophic pathogen that causes Sclerotinia stem rot in more than 700 species, resulting in serious economic losses. How S. sclerotiorum integrates mechanisms for nutrient acquisition and utilisation to maintain optimal growth and pathogenicity is still indistinct. Here, we demonstrate that Ndt80 family transcription factors (SsNdt80a,b,c) are involved in carbon source utilisation and have different roles in the growth, sclerotia formation, infection cushion development, and the virulence of S. sclerotiorum. SsNdt80b could bind the promoter of SsSNF1 and modulate the transcriptional activity of SsSNF1. Silencing SsSNF1 resulted in defects in hyphal growth and infection cushion formation, reduced cell wall-degrading enzymes, and reduced pathogenicity of S. sclerotiorum. A model is proposed in which SsNdt80b responds to carbon sources and modulates SsSnf1 to regulate the development and pathogenicity of S. sclerotiorum.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70088"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verticillium dahliae Secretory Aspartyl Protease VdSAP Targets Cotton GhARP to Modulate Plant Defence and Defoliation. 大丽黄萎病菌分泌的天冬氨酸蛋白酶VdSAP靶向棉花GhARP调控植物防御和落叶。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70085

Haiyuan Li, Xiaotong Gai, Xiangming Xu, Jun Qin, Steve J Klosterman, Krishna V Subbarao, Wenjing Shang, Xiaoping Hu

{"title":"Verticillium dahliae Secretory Aspartyl Protease VdSAP Targets Cotton GhARP to Modulate Plant Defence and Defoliation.","authors":"Haiyuan Li, Xiaotong Gai, Xiangming Xu, Jun Qin, Steve J Klosterman, Krishna V Subbarao, Wenjing Shang, Xiaoping Hu","doi":"10.1111/mpp.70085","DOIUrl":"https://doi.org/10.1111/mpp.70085","url":null,"abstract":"Verticillium dahliae isolates causing devastating vascular wilt in cotton plants can be divided into defoliating and nondefoliating pathotypes. The mechanisms underlying how V. dahliae uses secretory proteins to manipulate plant physiological processes and suppress immunity in cotton plants have received renewed research focus over the past several years. Here, we describe the role of a secretory protein named VdSAP (secretory aspartyl protease) from the defoliating V. dahliae strain XJ592 in virulence and defoliation. Deletion of VdSAP in strain XJ592 led to significantly reduced virulence and cotton plant defoliation. VdSAP functioned as a protease that targeted cotton plant GhARP (auxin-repressed protein), and VdSAP negatively regulated GhARP content in plants. Transient expression of GhARP in Nicotiana benthamiana enhanced the expression of plant defence signals and inhibited plant abscission signals. Furthermore, GhARP negatively regulated the ethylene (ET) signal and positively regulated the salicylic acid (SA) signal. In addition, GhARP interacted with 1-aminocyclopropane carboxylate oxidases (GhACOs), suggesting that it might function through the ET signal during V. dahliae-cotton plant interactions. These results suggest that GhARP is a molecular link between plant defence and abscission signals, and that VdSAP decreases accumulation of GhARP and enhances the virulence and defoliation caused by V. dahliae.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70085"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12008774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

lncRNA-Encoded Small Peptide Promotes Viral Infection. lncrna编码小肽促进病毒感染。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70084

Yaoyao Li, Juan Zhang, Chunyan Qi, Qitao Mo, Kaili Zhong, Jiaqian Liu, Hailin Cai, Jianyong Li, Jianping Chen, Jian Yang, Peng Liu

{"title":"lncRNA-Encoded Small Peptide Promotes Viral Infection.","authors":"Yaoyao Li, Juan Zhang, Chunyan Qi, Qitao Mo, Kaili Zhong, Jiaqian Liu, Hailin Cai, Jianyong Li, Jianping Chen, Jian Yang, Peng Liu","doi":"10.1111/mpp.70084","DOIUrl":"https://doi.org/10.1111/mpp.70084","url":null,"abstract":"The small open reading frames (ORFs) embedded in lncRNA have been identified to encode biologically functional peptides in many species. However, the function of lncRNA-encoded small peptides in the plant antiviral response remains unclear. In this study, an lncRNA Talnc54748 was identified, which encodes the small peptide ORF4. Transgenic Nicotiana benthamiana overexpressing ORF4 could enhance wheat yellow mosaic virus (WYMV) infection. RNA-sequencing analysis revealed that many genes in plant hormone signalling and MAPK signalling pathways were reduced in ORF4-overexpressing lines. The function of SAUR21 and MKK2, involved in plant hormone signalling and MAPK signalling pathways, respectively, was investigated by virus-induced gene silencing assay. Silencing SAUR21 or MKK2 in wheat and N. benthamiana enhanced WYMV infection. Transgenic overexpression of ORF4 not only enhanced the accumulation of tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV), but also promoted Phytophthora capsici or Pseudomonas syringae pv. tomato DC3000 infection. Our results demonstrated that an lncRNA-encoded small peptide plays an important role in plant immunity and provides new insights into the roles of this lncRNA-encoded small peptide in the plant antiviral response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70084"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12004088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi. 谷物锈菌生物学及毒力研究之基因组学研究。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-04-01 DOI: 10.1111/mpp.70082

Haixia Guan, Peng Zhang, Robert F Park, Yi Ding

{"title":"Genomics Research on the Road of Studying Biology and Virulence of Cereal Rust Fungi.","authors":"Haixia Guan, Peng Zhang, Robert F Park, Yi Ding","doi":"10.1111/mpp.70082","DOIUrl":"10.1111/mpp.70082","url":null,"abstract":"Rust fungi are highly destructive pathogens that pose a significant threat to crop production worldwide, especially cereals. Obligate biotrophy and, in many cases, complex life cycles make rust fungi particularly challenging to study. However, recent rapid advances in sequencing technologies and genomic analysis tools have revolutionised rust fungal research. It is anticipated that the increasing availability and ongoing substantial improvements in genome assemblies will propel the field of rust biology into the post-genomic era, instigating a cascade of research endeavours encompassing multi-omics and gene discoveries. This is especially the case for many cereal rust pathogens, for which continental-scale studies of virulence have been conducted over many years and historical collections of viable isolates have been sequenced and assembled. Genomic analysis plays a crucial role in uncovering the underlying causes of the high variability of virulence and the complexity of population dynamics in rust fungi. Here, we provide an overview of progress in rust genomics, discuss the strategies employed in genomic analysis, and elucidate the strides that will drive cereal rust biology into the post-genomic era.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 4","pages":"e70082"},"PeriodicalIF":4.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins. 来自不同王国的松树叶片病原体共享防御诱导效应蛋白。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70065

Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw

{"title":"Foliar Pine Pathogens From Different Kingdoms Share Defence-Eliciting Effector Proteins.","authors":"Mariana Tarallo, Carl H Mesarich, Rebecca L McDougal, Rosie E Bradshaw","doi":"10.1111/mpp.70065","DOIUrl":"10.1111/mpp.70065","url":null,"abstract":"Dothistroma needle blight, Cyclaneusma needle blight and red needle cast are devastating foliar pine diseases caused by the fungi Dothistroma septosporum and Cyclaneusma minus and the oomycete Phytophthora pluvialis, respectively. These pathogens colonise the host apoplast, secreting effector proteins to promote infection and disease. If these effectors are recognised by corresponding host resistance proteins, they activate the plant immune system to stop pathogen growth. We aimed to identify and characterise effectors that are common to all three pathogens. Using D. septosporum as a starting point, three candidate effectors (CEs) were investigated: Ds69335 (a CAP protein) and Ds131885, both of which have sequence and structural similarity to CEs of C. minus and P. pluvialis, and Ds74283, which adopts a β-trefoil fold and has structural rather than sequence similarity to CEs from all three pathogens. Notably, of the CEs investigated, Ds74283 and Ds131885, as well as their homologues from C. minus and P. pluvialis, elicited chlorosis or cell death in Nicotiana species, with Ds131885 and its homologues also triggering cell death in Pinus radiata. In line with these observed responses being related to activation of the plant immune system, the chlorosis triggered by Ds131885 and its homologues was compromised in a Nicotiana benthamiana mutant lacking the extracellular immune system co-receptor, SOBIR1. Such cross-kingdom, plant immune system-activating effectors, whether similar in sequence or structure, might ultimately enable the selection or engineering of durable, broad-spectrum resistance against foliar pine pathogens.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70065"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective Degradation of Cucumber Mosaic Virus RNA3 by Nonsense-Mediated Decay Benefits Viral Early Infection. 黄瓜花叶病毒RNA3无义衰变选择性降解有利于病毒早期感染。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70070

Danqing Zhao, Md Robel Ahmed, Mengjie Tian, Mengjiao Li, Zhouhang Gu, Qiansheng Liao, Zhiyou Du

{"title":"Selective Degradation of Cucumber Mosaic Virus RNA3 by Nonsense-Mediated Decay Benefits Viral Early Infection.","authors":"Danqing Zhao, Md Robel Ahmed, Mengjie Tian, Mengjiao Li, Zhouhang Gu, Qiansheng Liao, Zhiyou Du","doi":"10.1111/mpp.70070","DOIUrl":"10.1111/mpp.70070","url":null,"abstract":"Nonsense-mediated mRNA decay (NMD) is a critical RNA quality control system in eukaryotes, also playing a role in defending against viral infections. However, research has primarily focused on nonsegmented viruses. To investigate the interaction between NMD and segmented RNA viruses, we used cucumber mosaic virus (CMV), which possesses a tripartite, single-stranded, positive-sense RNA genome. Agroinfiltration assays were performed to assess how CMV RNA segments, or their variants, respond to NMD. We found that CMV genomic segments (RNAs 1-3) exhibit distinct responses to NMD. Specifically, RNA3, which serves as the translation template of the movement protein (MP), is selectively degraded by NMD, unlike RNA1 and RNA2, which encode viral replicase components. This degradation is triggered by the coat protein (CP) sequence and can be mitigated by the trans-expression of the 1a replicase or CP. The 1a protein requires its specific interaction with the Box-B motif of RNA3 to avoid NMD. Importantly, compromising NMD reduces CMV infection during the early stages, suggesting that NMD-mediated RNA3 degradation facilitates initial viral replication. This is supported by observations that MP expression in trans negatively regulates viral RNA replication. We propose a model to illustrate the molecular interplay between NMD and CMV, emphasising the implications of genomic segmentation in NMD-virus interactions.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70070"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication. TbCSV C3 蛋白中 C 端 7 个氨基酸基团的作用及其与 NbPOLA2 在增强病毒复制中的相互作用

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70068

Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing

{"title":"The Role of a C-Terminal Seven-Amino Acid Motif in TbCSV C3 Protein and Its Interaction With NbPOLA2 in Enhancing Viral Replication.","authors":"Mingjun Li, Puxin Huang, Zhou Jia, Xinyuan Lang, Lyuxin Wang, Miao Sun, Hussein Ghanem, Gentu Wu, Ling Qing","doi":"10.1111/mpp.70068","DOIUrl":"10.1111/mpp.70068","url":null,"abstract":"The C3 protein of tobacco curly shoot virus (TbCSV), a possible evolutionary intermediate between truly monopartite begomoviruses and those requiring satellite molecules for infectivity, has been identified as a viral replication enhancer (REn). However, the mechanisms underlying this enhancement are largely unknown. In this study, we generated two mutant infectious clones of TbCSV: one with a deletion of the 3' end region of the C3 gene that does not overlap with C2 (TbCSVdC3) and another in which this region was replaced by a phylogenetically unrelated iLOV gene sequence (TbCSVdC3-iLOV). Our findings highlight the crucial role of the 3' end region of C3 for viral DNA accumulation and further demonstrated that overexpression of TbCSV C3 protein in trans complements the functional deficiency of TbCSVdC3. Further analyses revealed the essential role of the C-terminal seven-amino acid motif from residues 123-129 of C3 in replication enhancement. Previous studies suggested that both intra- and intermolecular interactions of C3/AC3 proteins encoded by some other geminiviruses are vital for their capacity to enhance replication. Interestingly, among the tested potential interactors, NbPOLA2, a subunit of DNA polymerase α, was confirmed to interact with C3 in yeast and in planta. Our findings indicated that NbPOLA2 positively regulates TbCSV replication and infection and that the seven-amino acid motif (residues 123-129) in C3 is required for recruiting NbPOLA2 to facilitate TbCSV replication by mediating the viral double-stranded DNA (dsDNA) replication intermediate synthesis. These findings contribute to our understanding of the mechanisms through which the C3 protein enhances TbCSV replication.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70068"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11872800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum. 茄枯丝核菌AG1 IA糖苷水解酶家族16酶RsEG146诱导烟草细胞死亡并引发防御反应

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-03-01 DOI: 10.1111/mpp.70075

Chen Chen, Dongyang Jiang, Xi Li, Xue Ji, Rui Yang, Yuwen Chen, Ying Chen, Shimin Zuo, Xijun Chen

{"title":"Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.","authors":"Chen Chen, Dongyang Jiang, Xi Li, Xue Ji, Rui Yang, Yuwen Chen, Ying Chen, Shimin Zuo, Xijun Chen","doi":"10.1111/mpp.70075","DOIUrl":"10.1111/mpp.70075","url":null,"abstract":"Rhizoctonia solani AG1 IA is a harmful necrotrophic fungus responsible for various crop diseases, including maize and rice sheath blight, which can lead to significant production losses. However, the pathogenic mechanisms and the roles of effectors in this pathogen remain poorly understood. In this study, we identified a glycoside hydrolase 16 family gene, RsEG146, from R. solani that was upregulated during its infection of Zea mays leaves. When transiently expressed through agroinfiltration, RsEG146 induced cell death in the leaves of tobacco (Nicotiana tabacum 'Samsun'). The predicted signal peptide of RsEG146 was essential for its cell death-inducing activity, while the conserved enzymic active site was not required. The chitin-binding domain was critical for the cell death-inducing activity of RsEG146, with Gly47 identified as the key residue. Substitution of Gly47 with aspartate, glutamate, or proline significantly impaired the cell death-inducing activity of RsEG146. Additionally, transient and heterogeneous expression of RsEG146 enhanced the pathogenicity of Botrytis cinerea on tobacco, and silencing this gene through spray-induced gene silencing (SIGS) reduced the severity of the disease in maize, indicating that RsEG146 functions as an effector. Furthermore, RsEG146 triggered a plant immune response in tobacco. This study demonstrates that RsEG146 is a potential effector and plays a crucial role in the interactions between R. solani AG1 IA and its host.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 3","pages":"e70075"},"PeriodicalIF":4.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11911542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0